Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000912,226,7440TG24.5% 194.5 / 16.7 94V409G (GTG→GGG) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base T (28/43);  new base G (23/0);  total (51/43)
Fisher's exact test for biased strand distribution p-value = 5.08e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 125 total aligned reads displayed.

GCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGG  >  AC_000091/2226655‑2226833
                                                                                         |                                                                                         
 cgcATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggtg                                                                                          <  2:648721/90‑1 (MQ=255)
 cgcATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggtg                                                                                          <  1:2650369/90‑1 (MQ=255)
 cgcATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggtg                                                                                          <  1:2124760/90‑1 (MQ=255)
  gcATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggtgg                                                                                         <  2:502833/90‑1 (MQ=255)
  gcATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggggg                                                                                         >  1:2500994/1‑90 (MQ=255)
   cATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtgggggg                                                                                        >  2:849360/1‑89 (MQ=255)
   cATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGa                                                                                        <  1:2602878/90‑1 (MQ=255)
    aTGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggggggt                                                                                       >  1:1048167/1‑88 (MQ=255)
    aTGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGat                                                                                       >  2:882866/1‑90 (MQ=255)
    aTGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGAGGCtggggggt                                                                                       >  2:219652/1‑88 (MQ=255)
     tGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGatt                                                                                      <  1:2771060/90‑1 (MQ=255)
        aaaaCTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGattatt                                                                                   <  2:2044477/90‑1 (MQ=255)
         aaaCTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGtttttt                                                                                  >  2:1155922/1‑90 (MQ=255)
          aaCTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGGGGGCGGGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTc                                                                                 >  2:2354202/1‑90 (MQ=255)
           aCTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCa                                                                                <  2:3194405/90‑1 (MQ=255)
             tGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCaaa                                                                              <  1:2173092/90‑1 (MQ=255)
               gcgcGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTCAAACa                                                                            >  1:1134785/1‑90 (MQ=255)
                cgcgATAAATACGAGCAACATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTTAAAAAg                                                                           >  2:1901199/1‑90 (MQ=255)
                 gcgATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAgg                                                                          <  2:1618065/90‑1 (MQ=255)
                   gATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGcc                                                                        <  2:217055/90‑1 (MQ=255)
                   gATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTTAAAAAAGcc                                                                        >  2:850277/1‑90 (MQ=255)
                    aTAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTCAAACAAGCCg                                                                       >  2:2576168/1‑90 (MQ=255)
                     tAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGGGTGGGCGAAGCCAAATCGTGGCTGGTGTATTATTTCAAACAGGCCGa                                                                      <  2:1421112/90‑1 (MQ=255)
                     tAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGa                                                                      >  1:3548/1‑90 (MQ=255)
                        aTACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAgg                                                                   <  1:537513/90‑1 (MQ=255)
                         tACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGc                                                                  <  1:847367/90‑1 (MQ=255)
                          aCGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCg                                                                 <  2:350710/90‑1 (MQ=255)
                          aCGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCg                                                                 <  2:2129048/90‑1 (MQ=255)
                           cGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGa                                                                <  1:3012152/90‑1 (MQ=255)
                           cGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTTAAACAGGCCGAAGGCGa                                                                >  2:1713027/1‑90 (MQ=255)
                            gAGCATCATCTGCTGTTAAAAATGGCGGGCGTTGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGAt                                                               >  2:2057364/1‑90 (MQ=255)
                            gAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGGTTATTTCAAACAGGCCGAAGGCGAt                                                               >  2:749788/1‑90 (MQ=255)
                            gAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACACGCCGAAGGCGAt                                                               >  1:2879073/1‑90 (MQ=255)
                               natcatcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATNNNGNCTGGGGGGTTTTTTTAAACAAGCCGAAGGGGATTTc                                                            >  2:1834517/2‑90 (MQ=255)
                                atcatcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTTTTTCAAACCAGCCGGAGGCGAatttt                                                           >  2:1774313/1‑88 (MQ=255)
                                atcatcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCt                                                           <  1:3435793/90‑1 (MQ=255)
                                atcatcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCt                                                           <  2:328729/90‑1 (MQ=255)
                                atcatcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGGGGTTTCt                                                           >  2:663816/1‑90 (MQ=255)
                                 tcatcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCtt                                                          <  1:1385920/90‑1 (MQ=255)
                                 tcatcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGGAGGGGGTTTCtt                                                          >  1:200935/1‑90 (MQ=255)
                                     ctgctgTTAAAAATGGCGGGCGATGGCGTGTGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTc                                                      <  2:2854262/90‑1 (MQ=255)
                                     ctgctgTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTc                                                      <  2:2791097/90‑1 (MQ=255)
                                     ctgctgTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTc                                                      <  2:828530/90‑1 (MQ=255)
                                     ctgctgTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTCAAACAGGCCGAAGGCGATTTCTTTGTc                                                      >  1:1232694/1‑90 (MQ=255)
                                      tgctgTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCt                                                     <  2:1684574/90‑1 (MQ=255)
                                      tgctgTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCt                                                     >  1:1734910/1‑90 (MQ=255)
                                       gctgTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGGTTTCTTTGTCTg                                                    >  1:1437161/1‑90 (MQ=255)
                                         tgTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGGGATTTCTTTGTCTGTa                                                  >  1:3161055/1‑90 (MQ=255)
                                          gTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTAc                                                 <  1:2600326/90‑1 (MQ=255)
                                           ttAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAAGCCGAAGGCGATTTTTTTGTTTGTACg                                                >  2:1502451/1‑90 (MQ=255)
                                            tAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGc                                               >  2:2270930/1‑90 (MQ=255)
                                                aaTGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCgga                                           <  1:891975/90‑1 (MQ=255)
                                                 aTGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCggag                                          <  2:2079458/90‑1 (MQ=255)
                                                  tGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCggagg                                         >  1:291086/1‑90 (MQ=255)
                                                   ggcgggcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCggagga                                        <  1:1939645/90‑1 (MQ=255)
                                                    gcgggcgATGGCGTGGTCGACGCCAAATCGTGGCTTGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTTTCTGTACGCCGGAGGaa                                       <  2:439348/90‑1 (MQ=255)
                                                    gcgggcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGaa                                       <  2:2228476/90‑1 (MQ=255)
                                                    gcgggcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGaa                                       <  1:1094027/90‑1 (MQ=255)
                                                    gcgggcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGaa                                       >  1:2516809/1‑90 (MQ=255)
                                                     cgggcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAg                                      <  2:546405/90‑1 (MQ=255)
                                                      gggcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAgg                                     >  1:1002585/1‑90 (MQ=255)
                                                      gggcgATGGCGGGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAgg                                     >  1:759627/1‑90 (MQ=255)
                                                        gcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGgca                                   <  2:3323585/90‑1 (MQ=255)
                                                         ggATGGCGTGGGAGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGgcag                                  <  2:2896760/89‑1 (MQ=255)
                                                         cgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGgcag                                  >  1:2980348/1‑90 (MQ=255)
                                                         cgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAAGCCGAAGGCGATTTCTTTTTCTTGACGCCGGAGgaaggaag                                  >  2:2773574/1‑90 (MQ=255)
                                                          gATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGgcagc                                 >  1:378633/1‑90 (MQ=255)
                                                          gATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGgcagc                                 >  1:3228452/1‑90 (MQ=255)
                                                            tGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCaa                               <  1:2520745/90‑1 (MQ=255)
                                                               cGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTTTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGc                            >  2:2991211/1‑90 (MQ=255)
                                                                gTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCg                           >  1:2865120/1‑90 (MQ=255)
                                                                gTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCg                           >  1:3406863/1‑90 (MQ=255)
                                                                   ggCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGttt                        >  2:1064755/1‑90 (MQ=255)
                                                                   ggCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGttt                        <  2:293690/90‑1 (MQ=255)
                                                                   ggCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTTTACGCCGGAGGGAGGCAGCAAAGCGttt                        >  2:2850178/1‑90 (MQ=255)
                                                                   ggCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGttt                        >  1:1933394/1‑90 (MQ=255)
                                                                    gCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGtttt                       <  1:900241/90‑1 (MQ=255)
                                                                     cGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGttttt                      >  2:506509/1‑90 (MQ=255)
                                                                      gAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTa                     <  2:1431043/90‑1 (MQ=255)
                                                                       aaGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTac                    >  1:2216712/1‑90 (MQ=255)
                                                                       aaGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTac                    <  1:476575/90‑1 (MQ=255)
                                                                       aaGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTac                    >  1:736611/1‑90 (MQ=255)
                                                                        aGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTaca                   <  1:3202468/90‑1 (MQ=255)
                                                                        aGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTaca                   <  1:2991769/90‑1 (MQ=255)
                                                                          ccAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACAcc                 >  2:2547896/1‑90 (MQ=255)
                                                                           cAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCg                <  1:897259/90‑1 (MQ=255)
                                                                           cAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCg                >  2:3433751/1‑90 (MQ=255)
                                                                            aaaTCGTGGCTGGTGGATTATTTCGAACAGGCCGAAGGCGATTTCTTTGTGTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGt               >  1:79449/1‑90 (MQ=255)
                                                                            aaaTCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGt               <  1:290381/90‑1 (MQ=255)
                                                                               tCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTc            <  2:2966728/90‑1 (MQ=255)
                                                                                cGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTcg           <  1:1352845/90‑1 (MQ=255)
                                                                                 gTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTcgc          >  1:3131920/1‑90 (MQ=255)
                                                                                  tGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTcgcc         <  2:1067008/90‑1 (MQ=255)
                                                                                  tGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTcgcc         >  2:1261918/1‑90 (MQ=255)
                                                                                      tggtggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTg     >  1:1648271/1‑90 (MQ=255)
                                                                                       ggtggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGc    >  1:3059387/1‑90 (MQ=255)
                                                                                       ggtggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGc    <  1:3045592/90‑1 (MQ=255)
                                                                                       ggtggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGc    <  1:2111502/90‑1 (MQ=255)
                                                                                        gtggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCg   <  1:2604011/90‑1 (MQ=255)
                                                                                         tggATTATTTCAAACAGGCGGAAGGCGTTTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCgg  >  2:1285069/1‑90 (MQ=255)
                                                                                         |                                                                                         
GCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGG  >  AC_000091/2226655‑2226833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑