Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000912,708,9350AC25.8% 221.8 / 17.0 93intergenic (‑267/‑141)rpoE/nadBRNA polymerase, sigma 24 (sigma E) factor/quinolinate synthase, L‑aspartate oxidase (B protein) subunit
Reads supporting (aligned to +/- strand):  ref base A (60/9);  new base C (0/24);  total (60/33)
Fisher's exact test for biased strand distribution p-value = 3.54e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 113 total aligned reads displayed.

CTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTATCC  >  AC_000091/2708847‑2709021
                                                                                        |                                                                                      
cTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTccac                                                                                       <  1:11589/90‑1 (MQ=255)
cTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTccac                                                                                       <  1:74097/90‑1 (MQ=255)
 tGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTTCCTGAAGAGTAACCCAACTGCCTTTTTATTTCACCACCTAATCCTccacc                                                                                      <  1:213241/90‑1 (MQ=255)
 tGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTccacc                                                                                      <  1:330078/90‑1 (MQ=255)
 tGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTccacc                                                                                      <  2:1982241/90‑1 (MQ=255)
     cATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCAccagcc                                                                                  >  1:2302736/1‑90 (MQ=255)
     cATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGAAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCAccagcc                                                                                  <  2:1311806/90‑1 (MQ=255)
         gCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTa                                                                              >  1:574896/1‑90 (MQ=255)
           aTTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCCCCTAACCCTCCACCAGCCAGTAAc                                                                            <  2:381145/90‑1 (MQ=255)
           aTTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAAc                                                                            >  2:3031816/1‑90 (MQ=255)
              aTCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCAACCAGCCAGTAACTtc                                                                         >  2:172646/1‑90 (MQ=255)
               tCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTtct                                                                        >  1:243143/1‑90 (MQ=255)
                cTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTACCCCAACGGCCTTTTTATTTCACCCCCTAATCCTCCCCCAGCCAGTAACTtctc                                                                       <  2:6115/90‑1 (MQ=255)
                 tgtgTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCCCCACCTAATCCCCCCCCAGCCAGTAACTTctct                                                                      <  2:1933819/90‑1 (MQ=255)
                  gtgtAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCtt                                                                     >  1:507683/1‑90 (MQ=255)
                     tAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCttttt                                                                  >  2:2584651/1‑90 (MQ=255)
                       gAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCCCCAGCCAGTAACTTCTCTTTTtct                                                                <  1:2132215/90‑1 (MQ=255)
                       gAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTtct                                                                >  2:1616317/1‑90 (MQ=255)
                       gAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTtct                                                                >  1:3436079/1‑90 (MQ=255)
                        aaaTGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTtctc                                                               >  1:2050017/1‑90 (MQ=255)
                         aaTGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCCCCACCTAATCCCCCCCCAGCCAGTAACTTCTCTTTTTCTcg                                                              <  2:1062599/90‑1 (MQ=255)
                         aaTGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcg                                                              >  2:2409047/1‑90 (MQ=255)
                         aaTGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcg                                                              >  1:1886679/1‑90 (MQ=255)
                          aTGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTAGTTCACCACCTAATCCTCAACCAGCCAGTAACTTCTCTTTTTATcgc                                                             >  2:2002486/1‑90 (MQ=255)
                                aTTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCt                                                       <  1:1022442/90‑1 (MQ=255)
                                aTTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCt                                                       >  1:221132/1‑90 (MQ=255)
                                aTTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCt                                                       >  1:856562/1‑90 (MQ=255)
                                  ttAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGc                                                     >  1:2462028/1‑90 (MQ=255)
                                  ttAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGc                                                     >  1:358511/1‑90 (MQ=255)
                                  ttAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGc                                                     >  2:804062/1‑90 (MQ=255)
                                   tAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCCCCACCTAACCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCg                                                    <  1:492734/90‑1 (MQ=255)
                                    aaCTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGt                                                   >  1:295748/1‑90 (MQ=255)
                                      cTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCa                                                 >  2:793430/1‑90 (MQ=255)
                                        gCCTGAAGAGTACCCCACCGGCCTTTTTATTTCCCCCCCTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGc                                               <  2:748791/90‑1 (MQ=255)
                                        gCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGc                                               <  2:3274525/90‑1 (MQ=255)
                                         ccTGAAGAGTACCCCACCGGCCTTTTTATTTCACCCCCTAATCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCg                                              <  1:1692657/90‑1 (MQ=255)
                                         ccTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCg                                              >  1:3176620/1‑90 (MQ=255)
                                         ccTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCg                                              >  2:620870/1‑90 (MQ=255)
                                         ccTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCg                                              >  1:912954/1‑90 (MQ=255)
                                          cTGAAGAGTAACCCACCGGCCTTTTTATTTCACCACCTAATCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCgt                                             <  1:1745767/90‑1 (MQ=255)
                                          cTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCgt                                             >  2:2593228/1‑90 (MQ=255)
                                          cTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCgt                                             >  1:3322526/1‑90 (MQ=255)
                                           tGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCgtg                                            >  1:583651/1‑90 (MQ=255)
                                           tGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCAACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCgtg                                            >  1:2187248/1‑90 (MQ=255)
                                             aaGAGTACCCCAACGGCCTTTTTATTTCCCCACCTAATCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGtt                                          <  2:3320859/90‑1 (MQ=255)
                                             aaGAGTACCCCAACGGCCTTTTTATTTCACCACCTAATCCCCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGtt                                          <  1:3249976/90‑1 (MQ=255)
                                             aaGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGtt                                          >  2:2762183/1‑90 (MQ=255)
                                              agagTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGttt                                         >  2:1744919/1‑90 (MQ=255)
                                               gcgTACCCCAACGGCCTTTTTTTTTCCCCCCCTAATCCTCCGCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTa                                        <  2:2680695/88‑1 (MQ=255)
                                               gaagaaCCCAACGGCCTTTTTATTTCACCACCAAATCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTa                                        <  2:234322/86‑1 (MQ=255)
                                                agTACCCCAACGGCCTTTTTATTTCACCCCCTACTCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAg                                       <  2:3446995/90‑1 (MQ=255)
                                                agTACCCCAACGGCCTTTTTATTTCACCACCTAATCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAg                                       <  1:2253676/90‑1 (MQ=255)
                                                agTAACCCAACGGCTTTTTTATTTCCCCACCTAATCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAg                                       <  2:1088279/90‑1 (MQ=255)
                                                agTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAg                                       >  1:3138018/1‑90 (MQ=255)
                                                 gTACCCCAACGGCCTTTTTATTCCCCCCCCTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGc                                      <  1:544605/90‑1 (MQ=255)
                                                   aaCCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCaa                                    >  2:549895/1‑90 (MQ=255)
                                                    aCCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAAc                                   >  2:1251887/1‑90 (MQ=255)
                                                    aCCCAACGGCCTTTTTATTTCACCACCTAATCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAAc                                   <  2:1351264/90‑1 (MQ=255)
                                                     cccAACGGCCTTTTTTTTTCCCCCCCTAATCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACt                                  <  2:894849/90‑1 (MQ=255)
                                                     cccAACGGCCTTTTTATTTCACCCCCTAATCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACt                                  <  1:2920391/90‑1 (MQ=255)
                                                     cccAACGGCCTTTTTATTTCACCACCTAATCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACt                                  <  1:3034739/90‑1 (MQ=255)
                                                     cccAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGGAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACt                                  >  2:1136781/1‑90 (MQ=255)
                                                      ccACCGGCCTTTTTATTTCACCCCCTAATCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTg                                 <  2:2469443/90‑1 (MQ=255)
                                                       cAACGGCTTTTTTATTTCCCCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGt                                <  1:154958/90‑1 (MQ=255)
                                                        aaCGGCCTTTTTATTTCACCACCTAATCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTa                               <  2:2661248/90‑1 (MQ=255)
                                                        aaCGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAAc                                   <  2:1898350/86‑1 (MQ=255)
                                                        aaCGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAAc                                   >  1:1898350/1‑86 (MQ=255)
                                                        aaCGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTa                               >  1:374997/1‑90 (MQ=255)
                                                          cGGCCTTTTTATTTCACCCCCTAATCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAAc                             <  1:1711087/90‑1 (MQ=255)
                                                           ggCTTTTTTATTCCCCCCCCTAACCCCCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACa                            <  1:3214343/90‑1 (MQ=255)
                                                            gCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACaa                           >  2:1698957/1‑90 (MQ=255)
                                                            gCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACaa                           >  2:3408505/1‑90 (MQ=255)
                                                             ccTTTTTATTTCCCCCCCTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACaaa                          <  2:1007657/90‑1 (MQ=255)
                                                              cTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAat                         >  2:1789701/1‑90 (MQ=255)
                                                                ttttATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAatat                       >  2:3344425/1‑90 (MQ=255)
                                                                 tttATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATAtt                      >  1:254258/1‑90 (MQ=255)
                                                                 tttATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATAtt                      >  1:1476735/1‑90 (MQ=255)
                                                                  ttATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTa                     >  1:125646/1‑90 (MQ=255)
                                                                   tATTTCCCCCCCTAATCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTaa                    <  2:2550320/90‑1 (MQ=255)
                                                                    aTTTCCCCACCTAATCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTaaa                   <  2:1970158/90‑1 (MQ=255)
                                                                      ttCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAAt                 >  1:968445/1‑90 (MQ=255)
                                                                       tCACCCCCTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATa                <  1:96850/90‑1 (MQ=255)
                                                                       tCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATa                >  2:12592/1‑90 (MQ=255)
                                                                         accaccTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGc              >  1:883127/1‑90 (MQ=255)
                                                                          cccccTAACCCCCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCa             <  2:2496216/87‑1 (MQ=255)
                                                                             ccTAATCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGgt          <  1:1282053/90‑1 (MQ=255)
                                                                             ccTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGgt          >  1:2058670/1‑90 (MQ=255)
                                                                             ccTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGgt          <  2:152986/90‑1 (MQ=255)
                                                                              cTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGgtg         <  2:1974968/90‑1 (MQ=255)
                                                                               tAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGgtgt        >  1:1292127/1‑90 (MQ=255)
                                                                                aaTCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGtt       >  1:3235016/1‑90 (MQ=255)
                                                                                aaTCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGtt       >  2:1710061/1‑90 (MQ=255)
                                                                                 aTCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGttt      >  1:105255/1‑90 (MQ=255)
                                                                                   cctccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTAt    >  1:1760823/1‑90 (MQ=255)
                                                                                   cctccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTAt    >  2:1078286/1‑90 (MQ=255)
                                                                                   cccccaccaGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTAt    <  1:898250/87‑1 (MQ=255)
                                                                                    ctccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTATc   >  2:1805992/1‑90 (MQ=255)
                                                                                    ctccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTATc   >  1:1430187/1‑90 (MQ=255)
                                                                                     tccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTATcc  <  1:275844/90‑1 (MQ=255)
                                                                                     tccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTATcc  >  1:133581/1‑90 (MQ=255)
                                                                                        |                                                                                      
CTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTATCC  >  AC_000091/2708847‑2709021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑