Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000912,817,0300GC27.4% 148.8 / 37.9 62intergenic (‑100/+99)argY/argVtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base G (9/36);  new base C (16/1);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 1.04e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTA  >  AC_000091/2816942‑2817119
                                                                                         |                                                                                         
tactttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGc                                                                                          >  2:1235330/1‑90 (MQ=255)
 actttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTcccc                                                                                         >  2:1830836/1‑90 (MQ=255)
 actttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGcc                                                                                         >  2:262118/1‑90 (MQ=255)
  ctttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCcccc                                                                                        >  1:107841/1‑90 (MQ=255)
  ctttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCcccc                                                                                        >  1:2223363/1‑90 (MQ=255)
   tttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCccccc                                                                                       >  2:2600673/1‑89 (MQ=255)
     ttctGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                     <  2:626176/88‑1 (MQ=255)
     tactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCccccccc                                                                                     >  1:692978/1‑87 (MQ=255)
     tactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTc                                                                                     >  2:131700/1‑90 (MQ=255)
      actGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCcccccccc                                                                                    >  1:1945832/1‑86 (MQ=255)
      actGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGccccccg                                                                                    >  2:1286054/1‑86 (MQ=255)
       ctGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCTTCgg                                                                                   >  2:143058/1‑90 (MQ=255)
        tGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCTTcccg                                                                                  >  2:863749/1‑87 (MQ=255)
        tGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCcgg                                                                                  >  1:2548945/1‑90 (MQ=17)
         gCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCCTCgggg                                                                                 >  2:2860577/1‑89 (MQ=255)
         gCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCCCgggg                                                                                 >  2:1324815/1‑89 (MQ=17)
          cAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCCTCggggc                                                                                >  2:1053420/1‑88 (MQ=255)
          cAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCGGGcg                                                                                >  1:2314721/1‑89 (MQ=255)
           aGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCGGGCCg                                                                               >  1:740623/1‑90 (MQ=255)
             atacttttATTCCGCTACTAAAGCAGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGtt                                                                             <  2:2281972/86‑1 (MQ=14)
             aTTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGGCggt                                                                             >  1:1602619/1‑88 (MQ=14)
              ttttttGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTg                                                                            <  1:2358300/90‑1 (MQ=255)
              ttttttGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCGGGCCGGTg                                                                            >  1:1252067/1‑90 (MQ=14)
               tttttGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCCTCGGGGCGGTGc                                                                           >  1:132867/1‑90 (MQ=14)
                ttttGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCt                                                                          <  2:285244/90‑1 (MQ=40)
                 tttGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCGGGGCGTTGGTa                                                                         >  2:2363829/1‑90 (MQ=14)
                  ttGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGCCGGTGCTaa                                                                        >  2:1088336/1‑90 (MQ=14)
                   tGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCTTCCGGCCGTGGCTtaa                                                                       >  2:2200757/1‑90 (MQ=14)
                   tGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGGCGGTGCTcaa                                                                       >  2:175824/1‑90 (MQ=14)
                     aTACCGCTACTAAAGCCGTATCAAGTAAGAGCTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGc                                                                     <  2:3440812/90‑1 (MQ=255)
                     aTACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCGGGGGGGTGCTGAAga                                                                     >  2:2992899/1‑89 (MQ=14)
                      tACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCAtt                                                                                      <  1:732055/72‑1 (MQ=255)
                       aCCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGGCGGTGGTTAAGGCa                                                                    >  2:154020/1‑90 (MQ=14)
                        ccGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                  <  1:2485867/90‑1 (MQ=38)
                         cGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCACCCTCCCGGCCGCTGCTGAAACAACg                                                                 >  2:1259975/1‑90 (MQ=11)
                          gCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCgg                                                                                   <  2:2415772/71‑1 (MQ=39)
                          gCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCCTCCGGCCCGTGCTTAAGaaaagc                                                                >  1:2579741/1‑87 (MQ=14)
                          gCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGGCGTTGCTCAAGCAACgg                                                                >  1:182551/1‑89 (MQ=14)
                           ctactaAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGCCGGTGCTCAAACAACAtt                                                               >  2:591326/1‑90 (MQ=11)
                            tactaAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCg                                                                                    <  2:1960511/68‑1 (MQ=40)
                            tactaAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCCTCGGGGGGGTGCTAAAGCAACgctg                                                              >  1:655393/1‑87 (MQ=14)
                            tactaAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCGGGCCGCTGCTAAAACAACATTa                                                              >  2:804099/1‑90 (MQ=11)
                             actaAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGGCGGGGCTTAAACAAAGTTAt                                                             >  2:1728266/1‑90 (MQ=14)
                               taAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAtcc                                                           <  1:366476/90‑1 (MQ=39)
                                aatgCCGTATCAGGTAAGAGATGGTGCATCCGGGAGGTTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAtcct                                                          <  2:3149345/87‑1 (MQ=14)
                                    ccGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCt                                                      <  1:2974844/90‑1 (MQ=255)
                                     cGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCCCCGGCCGCTGGTTAAGCAACGTTTTTCTccccc                                                     >  1:2608854/1‑88 (MQ=14)
                                      gTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGCCGGTGCTATACCAACGTTATCCCCCCtcg                                                    >  1:3311196/1‑88 (MQ=14)
                                       tATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                                           <  2:1283063/50‑1 (MQ=38)
                                       tATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGCCGCTGCCAAAGAAACGGTATTCTCCCCggc                                                   >  1:869580/1‑89 (MQ=11)
                                            aGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGGCGGTGGTTAAACAACGCTATTCTCCCCCGTGcccg                                              >  2:94561/1‑88 (MQ=2)
                                            aGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGCCGGCGCTAAAACAACGTTATCCTCCCCCGTGcccc                                              >  1:2239428/1‑88 (MQ=11)
                                             ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCCCCGGCCGGTGCTTAAACAACGTTTTTCTCCCCCGTGCCCGc                                             >  2:2835502/2‑90 (MQ=2)
                                              tAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCCTCCGGGCGGTGCTAAAACAACAGTATTCTCCCCCGTGCCTGCg                                            >  1:1423701/1‑90 (MQ=11)
                                               aaGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCgg                                           <  1:2835502/90‑1 (MQ=39)
                                               aaGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCgg                                           <  1:153386/90‑1 (MQ=38)
                                                      ggTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTAc                                    <  1:1853240/90‑1 (MQ=255)
                                                            tCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCAc                              <  1:589430/90‑1 (MQ=255)
                                                             ccGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCAcc                             <  1:554096/90‑1 (MQ=255)
                                                              cGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACct                            <  1:1318323/90‑1 (MQ=255)
                                                              cGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACct                            <  1:3155645/90‑1 (MQ=255)
                                                               gggAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACctc                           <  1:2727928/90‑1 (MQ=255)
                                                                  aggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTc                        <  2:2163739/90‑1 (MQ=255)
                                                                   ggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTcc                       <  2:730558/90‑1 (MQ=255)
                                                                    gaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCa                      <  1:1598610/90‑1 (MQ=255)
                                                                     aTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCAt                     <  1:997140/90‑1 (MQ=255)
                                                                     aTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCAt                     <  1:678823/90‑1 (MQ=255)
                                                                     aTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCAt                     <  1:352162/90‑1 (MQ=255)
                                                                      ttACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATa                    <  1:1353803/90‑1 (MQ=255)
                                                                       tACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATAc                   <  2:260275/90‑1 (MQ=255)
                                                                           cGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTc               <  2:3344694/90‑1 (MQ=255)
                                                                            gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCa              <  2:2514706/90‑1 (MQ=255)
                                                                            gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCa              <  2:573293/90‑1 (MQ=255)
                                                                                 gCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCt         <  1:1726653/90‑1 (MQ=255)
                                                                                  cGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTg        <  1:2665480/90‑1 (MQ=255)
                                                                                   ggCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCAGCTCTTCCATACCTTCATCGCTGa       >  2:3252650/1‑90 (MQ=255)
                                                                                   ggCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGa       <  2:1319092/90‑1 (MQ=255)
                                                                                    gCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGaa      <  2:3035464/90‑1 (MQ=255)
                                                                                     cTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGaaa     >  2:595066/1‑90 (MQ=255)
                                                                                     cTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGaaa     >  1:2688768/1‑90 (MQ=255)
                                                                                      tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAg    <  2:1077159/90‑1 (MQ=255)
                                                                                      tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAg    <  1:1901299/90‑1 (MQ=255)
                                                                                      tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAg    >  2:1324158/1‑90 (MQ=255)
                                                                                        cGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGt   >  1:1334646/1‑90 (MQ=255)
                                                                                         gCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTa  >  1:403804/1‑90 (MQ=255)
                                                                                         |                                                                                         
TACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTA  >  AC_000091/2816942‑2817119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: