Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000912,987,5350AC26.6% 297.7 / 26.0 129P126P (CCA→CCC) yqeIpredicted transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (84/10);  new base C (0/34);  total (84/45)
Fisher's exact test for biased strand distribution p-value = 2.07e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 140 total aligned reads displayed.

GAGAAGAGGATTCAAGATCCATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATAAAAAGTC  >  AC_000091/2987448‑2987623
                                                                                       |                                                                                         
gagaAGAGGATTCAAGATCCATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑cc                                                                                        >  1:1023413/1‑90 (MQ=255)
 agaagaGGATTCAAGATCCATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCt                                                                                       <  2:487434/90‑1 (MQ=255)
   aagaGGATTCAAGATCCATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGc                                                                                     >  1:1229086/1‑90 (MQ=255)
     gagGATTCAAGATCCATAATGACATTACCATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCa                                                                                   <  2:2841616/90‑1 (MQ=255)
     gagGATTCAAGATCCATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCa                                                                                   >  1:1781166/1‑90 (MQ=255)
      agGATTCAAGATCCATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCAt                                                                                  <  1:343831/90‑1 (MQ=255)
      agGATTCAAGATCCATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCAt                                                                                  >  2:3441748/1‑90 (MQ=255)
        gATTCAAGATCCATAATGACATTAACATACAAAATCATGTAATGAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATaa                                                                                >  2:2164482/1‑90 (MQ=255)
         aTTCAAGATCCATAATGACATTAAGATACAAAATCATGTAATAAACTCGTGAACAGACGCACATACACACAATGCCCCA‑CCTGCCATaaa                                                                               >  1:3418715/1‑90 (MQ=255)
            cAAGATCCATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGt                                                                            <  1:940983/90‑1 (MQ=255)
                aTCCATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATGAAAGTTAAt                                                                        >  1:1230571/1‑90 (MQ=255)
                  ccATAATGACATTAACATACAAAATCATGTAATAACCTCGTCAACAGACGCACATACACACAATGCCCCC‑CCTGCCATAAAAGTTAATGc                                                                      <  1:3224482/90‑1 (MQ=255)
                  ccATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCC‑CCTGCCATAAAAGTTAATGc                                                                      <  2:1297233/90‑1 (MQ=255)
                   cATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATCCACACAATCCCCCC‑CCTGCCATAAAAGTTAATGCg                                                                     <  2:2674415/90‑1 (MQ=255)
                      aaTGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGa                                                                  >  2:2724193/1‑90 (MQ=255)
                       aTGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGAt                                                                 >  2:2262556/1‑90 (MQ=255)
                           cATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACaa                                                             >  2:898388/1‑90 (MQ=255)
                            aTTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACaaa                                                            >  2:3206046/1‑90 (MQ=255)
                            aTTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACaaa                                                            >  1:2904551/1‑90 (MQ=255)
                             ttAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAag                                                           >  1:934877/1‑90 (MQ=255)
                             ttAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAag                                                           >  1:305557/1‑90 (MQ=255)
                                aCATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAagaga                                                        >  2:3344052/1‑90 (MQ=255)
                                   tACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCa                                                     >  1:2243624/1‑90 (MQ=255)
                                   tACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCa                                                     >  1:1601572/1‑90 (MQ=255)
                                       aaaTCATGTAATAAACTCGTCAACAGACGGACATACACCCAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATtgg                                                 >  2:1577451/1‑90 (MQ=255)
                                       aaaTCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATtgg                                                 >  2:1752952/1‑90 (MQ=255)
                                        aaTCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATtggt                                                <  2:1136137/90‑1 (MQ=255)
                                        aaTCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATtggt                                                >  2:312375/1‑90 (MQ=255)
                                        aaTCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATtggt                                                >  1:2750733/1‑90 (MQ=255)
                                        aaTCATGTAAAAAACTCGTCAACAGACGCACATACACACAAGCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATtggt                                                <  1:3204704/90‑1 (MQ=255)
                                         aTCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATtggtg                                               >  1:2233844/1‑90 (MQ=255)
                                          tCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATtggtgg                                              >  1:2722563/1‑90 (MQ=255)
                                          tCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATtggtgg                                              >  1:3133205/1‑90 (MQ=255)
                                          tCATGTAAAAACCTCGTCAACAGCCCCACATACACACAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATtggtgg                                              <  2:1391834/90‑1 (MQ=255)
                                           cATGTAATAAACTCGTCAACAGACCCACATACACACAATGCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGgc                                             <  2:1939964/90‑1 (MQ=255)
                                            ttGTAAAAAACTCGTCAACAGACGCACAACCCCACATTCCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGgcg                                            <  1:2053785/89‑1 (MQ=255)
                                             tGTAATAACCTCTTCAACAGACCCACATACACACAAGGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGgcgc                                           <  1:577350/90‑1 (MQ=255)
                                             tGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGgcgc                                           >  1:2242568/1‑90 (MQ=255)
                                              gTAATAACCTCGTCAACAGACCCACATCCACACAATGCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCa                                          <  1:1037508/90‑1 (MQ=255)
                                              gTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCa                                          >  1:784394/1‑90 (MQ=255)
                                               taataaCCTCGTCAACAGACGCACATACACACAATGCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCaa                                         <  1:2407649/90‑1 (MQ=255)
                                                 ataaACCCGTCAACAGACCCACATACCCACAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAg                                       <  2:2135797/90‑1 (MQ=255)
                                                  taaACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGa                                      >  1:2167788/1‑90 (MQ=255)
                                                  taaACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGa                                      >  2:2453674/1‑90 (MQ=255)
                                                   aaaCTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGaa                                     >  1:302906/1‑90 (MQ=255)
                                                   aaaCTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGaa                                     >  2:794361/1‑90 (MQ=255)
                                                   aaaCCCGTCAACAGACGCACATACACACAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGaa                                     <  2:1017102/90‑1 (MQ=255)
                                                    aaCTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAAt                                    >  1:2435115/1‑90 (MQ=255)
                                                    aaCTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAAt                                    >  2:1216331/1‑90 (MQ=255)
                                                     aCTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAAtt                                   >  1:3430667/1‑90 (MQ=255)
                                                      cTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAAttt                                  >  2:3095495/1‑90 (MQ=255)
                                                      cTCGTCAACAGACGCACATACACACAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAAttt                                  <  2:1419158/90‑1 (MQ=255)
                                                       tCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTc                                 >  2:1022145/1‑90 (MQ=255)
                                                        cGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCa                                >  1:1005759/1‑90 (MQ=255)
                                                        cGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCa                                >  1:1265329/1‑90 (MQ=255)
                                                        cGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGGGGCGCAAAGAATTTCa                                >  2:2921183/1‑90 (MQ=255)
                                                         gtaaaCAGACGCACATCCACACAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCaa                               <  1:2123271/87‑1 (MQ=255)
                                                         gTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCaa                               >  1:524108/1‑90 (MQ=255)
                                                         gTCAACAGACGCACATACACACAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCaa                               <  1:1341999/90‑1 (MQ=255)
                                                         gTCAACAGACGCACATACACACAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCaa                               <  2:2182582/90‑1 (MQ=255)
                                                          tCAACAGCCCCACATACACACAATGCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAAt                              <  2:897401/90‑1 (MQ=255)
                                                          tCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGAGACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAAt                              >  2:655116/1‑90 (MQ=255)
                                                           cAACAGACCCACATACACACAACGCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATa                             <  1:98270/90‑1 (MQ=255)
                                                            aaCAGACGCACATACACACAATGCCCCA‑CCTCCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATaa                            >  1:1112969/1‑90 (MQ=255)
                                                             aCAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAAc                           >  2:754475/1‑90 (MQ=255)
                                                              cAGACGCACATACACACAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACa                          <  2:1994893/90‑1 (MQ=255)
                                                               aGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACaa                         >  1:1234678/1‑90 (MQ=255)
                                                               aGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACaa                         >  2:3302300/1‑90 (MQ=255)
                                                               aGACGCACATACACACAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACaa                         <  2:252604/90‑1 (MQ=255)
                                                                gACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACaaa                        >  2:747365/1‑90 (MQ=255)
                                                                gACGCACATACACACAAGGCCCCG‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACaaa                        <  1:2494994/90‑1 (MQ=255)
                                                                  cGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAAt                      >  2:3028173/1‑90 (MQ=255)
                                                                   gCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATc                     >  2:2263507/1‑90 (MQ=255)
                                                                   gCACATACACACAACCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATc                     <  1:1954930/90‑1 (MQ=255)
                                                                   ccacaTCCACACAATGCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATc                     <  1:1769610/89‑1 (MQ=255)
                                                                   ccacaTACACACAACCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATc                     <  2:3443701/89‑1 (MQ=255)
                                                                    cacaTACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATcc                    >  2:2804398/1‑90 (MQ=255)
                                                                     acaTACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCt                   >  1:2637330/1‑90 (MQ=255)
                                                                     acaTACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCt                   >  2:1512138/1‑90 (MQ=255)
                                                                      caaacacacAATGCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTc                  <  1:2311087/87‑1 (MQ=255)
                                                                      caTACACACAATGCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTc                  <  2:1604196/90‑1 (MQ=255)
                                                                      caTACACACAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTc                  <  1:101922/90‑1 (MQ=255)
                                                                       aTACACACAAGGCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCa                 <  1:2169706/90‑1 (MQ=255)
                                                                        tACACACAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCaa                <  1:3102172/90‑1 (MQ=255)
                                                                         acacacAATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCaaa               <  1:1574992/90‑1 (MQ=255)
                                                                            cacaATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACat            >  1:2336749/1‑90 (MQ=255)
                                                                            cacaATCCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACat            <  1:1439417/90‑1 (MQ=255)
                                                                             acaATGCCCCC‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACata           <  2:345514/90‑1 (MQ=255)
                                                                             acaATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACata           >  1:975050/1‑90 (MQ=255)
                                                                               aaTGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACAtata         >  2:1806274/1‑90 (MQ=255)
                                                                                aTGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATaa        >  2:1022771/1‑90 (MQ=255)
                                                                                 tGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATaaa       >  1:3023183/1‑90 (MQ=255)
                                                                                  cccccc‑cCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATaaaa      <  2:1908585/89‑1 (MQ=255)
                                                                                   ccccACCCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTTGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATaaaa      <  2:1604239/90‑1 (MQ=255)
                                                                                   ccccA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATaaaaa     >  2:1516287/1‑90 (MQ=255)
                                                                                    cccc‑ccTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATAAAAAg    <  2:2952756/90‑1 (MQ=255)
                                                                                    cccc‑ccTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATAAAAAg    <  2:3376216/90‑1 (MQ=255)
                                                                                    cccA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATAAAAAg    >  1:2602668/1‑90 (MQ=255)
                                                                                    cccA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATAAAAAg    >  2:2471165/1‑90 (MQ=255)
                                                                                      cA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATAAAAAGTc  >  2:291442/1‑90 (MQ=255)
                                                                                       |                                                                                         
GAGAAGAGGATTCAAGATCCATAATGACATTAACATACAAAATCATGTAATAAACTCGTCAACAGACGCACATACACACAATGCCCCA‑CCTGCCATAAAAGTTAATGCGGGATACAAAGAGAGCATTGGTGGCGCAAAGAATTTCAATAACAAAATCCTCAAACATATAAAAAGTC  >  AC_000091/2987448‑2987623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑