Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000913,259,7110AC29.0% 199.5 / 16.6 93V455G (GTG→GGG) tdcEpyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (57/9);  new base C (0/27);  total (57/36)
Fisher's exact test for biased strand distribution p-value = 4.82e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 119 total aligned reads displayed.

CGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTG  >  AC_000091/3259622‑3259800
                                                                                         |                                                                                         
cGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCa                                                                                           >  1:2325025/1‑90 (MQ=255)
cGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCa                                                                                           >  1:3063959/1‑90 (MQ=255)
cGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCa                                                                                           >  1:986405/1‑90 (MQ=255)
 gAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAc                                                                                          >  2:3017790/1‑90 (MQ=255)
   ggCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAccc                                                                                        <  2:2569543/90‑1 (MQ=255)
   ggCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAccc                                                                                        <  2:3443216/90‑1 (MQ=255)
    gCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAcccc                                                                                       <  2:2002596/90‑1 (MQ=255)
     cTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccg                                                                                      <  1:1441340/90‑1 (MQ=255)
     cTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccg                                                                                      <  1:2913620/90‑1 (MQ=255)
     cTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccg                                                                                      >  1:2503412/1‑90 (MQ=255)
      tGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAcgccgc                                                                                     >  1:1032671/1‑90 (MQ=255)
           aTCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTa                                                                                >  2:27344/1‑90 (MQ=255)
            tCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGGGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTaa                                                                               >  2:3317649/1‑90 (MQ=255)
              aCTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAAtt                                                                             >  2:817886/1‑90 (MQ=255)
               cTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTg                                                                            >  1:622664/1‑90 (MQ=255)
                 ttGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGTATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCg                                                                          <  2:334126/90‑1 (MQ=255)
                   gTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTa                                                                        >  2:855572/1‑90 (MQ=255)
                   gTCGTAATCCAGCACGTAGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTa                                                                        >  1:2522758/1‑90 (MQ=255)
                   gTCGTAATCCAGCACGACGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCCCCGTTAATTGCgga                                                                        >  2:545840/1‑88 (MQ=255)
                     cGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTaga                                                                      >  1:1374561/1‑90 (MQ=255)
                      gTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTagag                                                                     <  2:1047094/90‑1 (MQ=255)
                      gTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTagag                                                                     <  1:1118847/90‑1 (MQ=255)
                       tAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGCCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGagc                                                                    <  1:634375/90‑1 (MQ=255)
                         aTCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGagcag                                                                  >  2:3155826/1‑90 (MQ=255)
                             aGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGtt                                                              <  1:317557/90‑1 (MQ=255)
                               cACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGtttt                                                            <  2:2860099/90‑1 (MQ=255)
                                aCGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTc                                                           >  2:1214920/1‑90 (MQ=255)
                                 cgtcgtCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCg                                                          <  2:1583179/90‑1 (MQ=255)
                                  gtcgtcCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGc                                                         <  1:358974/90‑1 (MQ=255)
                                  gtcgtcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGc                                                         >  2:752230/1‑90 (MQ=255)
                                   tcgtcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGcc                                                        >  2:2584041/1‑90 (MQ=255)
                                   ncgtcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTATNNNCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGcc                                                        >  2:2809816/2‑90 (MQ=255)
                                    cgtcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCa                                                       >  2:1104552/1‑90 (MQ=255)
                                     gtcCATCAGCGGTGCTTTTTTCGGCCCGACCTGAATCTTCAGCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAg                                                      <  1:3085327/90‑1 (MQ=255)
                                     gtcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAg                                                      <  2:199439/90‑1 (MQ=255)
                                         aTCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTa                                                  >  2:2185574/1‑90 (MQ=255)
                                          tCAGCGGTGCTGTTTTCGCCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAg                                                 <  1:896188/90‑1 (MQ=39)
                                           cAGCGGTGCTTTTTTCGGCCCGCCCTTAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgc                                                <  2:3259528/90‑1 (MQ=39)
                                           cAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgc                                                <  1:1955895/90‑1 (MQ=255)
                                           cAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgc                                                <  2:2606548/90‑1 (MQ=255)
                                           cAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGGTAgc                                                >  2:1736288/1‑90 (MQ=255)
                                            aGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcg                                               >  2:1417803/1‑90 (MQ=255)
                                             gCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcgc                                              <  1:14630/90‑1 (MQ=255)
                                               ggTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCCCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGt                                            <  1:2573371/90‑1 (MQ=255)
                                               ggTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGt                                            <  2:1990112/90‑1 (MQ=255)
                                                 tGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGc                                          >  2:2206664/1‑90 (MQ=255)
                                                 tGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGc                                          >  1:2805293/1‑90 (MQ=255)
                                                  gCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCa                                         >  2:1283248/1‑90 (MQ=255)
                                                  gCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCa                                         >  1:1072862/1‑90 (MQ=255)
                                                   ttttttcCGGCCCCACCTGACTTTCCCTCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAc                                        <  2:1488859/87‑1 (MQ=25)
                                                    tGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAcc                                       >  1:2869386/1‑90 (MQ=255)
                                                     gTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCa                                      <  2:389232/90‑1 (MQ=255)
                                                       tttCGGCCCGACCTGAATTTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaa                                    <  2:1682901/90‑1 (MQ=37)
                                                        ttCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAg                                   >  1:102541/1‑90 (MQ=255)
                                                        ttCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAg                                   >  2:418409/1‑90 (MQ=255)
                                                        ttCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAg                                   >  1:1714917/1‑90 (MQ=255)
                                                        ttCGCCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAg                                   <  2:3083342/90‑1 (MQ=255)
                                                            gCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACt                               >  1:140212/1‑90 (MQ=255)
                                                            gCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACt                               <  2:455393/90‑1 (MQ=255)
                                                             cccGACCTGAATCTTAAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTg                              >  2:2215660/1‑90 (MQ=255)
                                                                gACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAGAGAACTGCAt                           >  2:2969756/1‑90 (MQ=255)
                                                                gACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt                           >  1:2473011/1‑90 (MQ=255)
                                                                gACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt                           >  2:2288392/1‑90 (MQ=255)
                                                                 cccTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt                          <  2:1870346/89‑1 (MQ=255)
                                                                  ccTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAttt                         <  2:3062864/90‑1 (MQ=255)
                                                                  ccTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAttt                         >  1:768821/1‑90 (MQ=255)
                                                                   cTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTg                        >  1:2961467/1‑90 (MQ=255)
                                                                    tGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGc                       <  2:2582664/90‑1 (MQ=255)
                                                                     gAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCt                      <  2:1622515/90‑1 (MQ=255)
                                                                      caTCTTCAGCTTCTTGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCACTTGCtt                     <  2:1453304/89‑1 (MQ=37)
                                                                      aaTCTTCAGTTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt                     <  2:329964/90‑1 (MQ=255)
                                                                      aaTCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt                     <  2:484211/90‑1 (MQ=255)
                                                                       aTCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTa                    <  1:204142/90‑1 (MQ=255)
                                                                       aTCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTa                    >  1:1284579/1‑90 (MQ=255)
                                                                         cTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc                  >  2:2049286/1‑90 (MQ=255)
                                                                          ttCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCa                 <  1:2534475/90‑1 (MQ=255)
                                                                          ttCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCa                 >  2:2562560/1‑90 (MQ=255)
                                                                           tCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCaa                >  2:3306143/1‑90 (MQ=255)
                                                                            cAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAAt               <  1:591380/90‑1 (MQ=255)
                                                                             aGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc              <  2:102880/90‑1 (MQ=255)
                                                                             aGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc              >  2:235953/1‑90 (MQ=255)
                                                                             aGCTGCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc              <  1:2368086/90‑1 (MQ=255)
                                                                              gCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCa             <  1:1088691/90‑1 (MQ=255)
                                                                               cTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCAc            >  1:1920958/1‑90 (MQ=255)
                                                                                ttCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCAcc           >  2:2873291/1‑90 (MQ=255)
                                                                                ttCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCAcc           <  2:2567888/90‑1 (MQ=255)
                                                                                ttCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCAcc           <  2:2394442/90‑1 (MQ=255)
                                                                                 tctcGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCa          >  1:2231068/1‑90 (MQ=255)
                                                                                  ctcGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCAt         >  1:2096764/1‑90 (MQ=255)
                                                                                  ctcGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCAt         >  2:1743863/1‑90 (MQ=255)
                                                                                   tcGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCAtt        <  2:407821/90‑1 (MQ=255)
                                                                                   tcGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCAtt        >  1:2156775/1‑90 (MQ=255)
                                                                                   tcGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCAtt        <  2:1305633/90‑1 (MQ=255)
                                                                                    cGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTg       >  2:381498/1‑90 (MQ=255)
                                                                                    cGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTg       >  2:2560069/1‑90 (MQ=255)
                                                                                     gTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTgg      <  2:1910128/90‑1 (MQ=255)
                                                                                     gTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTgg      <  2:3035091/90‑1 (MQ=255)
                                                                                      tCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTggg     <  2:2541137/90‑1 (MQ=255)
                                                                                        cACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCt   >  1:1123677/1‑90 (MQ=255)
                                                                                         aCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTg  >  2:2263917/1‑90 (MQ=255)
                                                                                         |                                                                                         
CGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTG  >  AC_000091/3259622‑3259800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑