Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000913,962,9680AC25.0% 327.0 / 12.5 146T307P (ACC→CCC) yhjGpredicted outer membrane biogenesis protein
Reads supporting (aligned to +/- strand):  ref base A (69/39);  new base C (0/36);  total (69/77)
Fisher's exact test for biased strand distribution p-value = 2.95e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 169 total aligned reads displayed.

TGAAGATGGGCGGTGTCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAATGGGCGA  >  AC_000091/3962879‑3963057
                                                                                         |                                                                                         
tGAAGATGGGCGGTGTCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATa                                                                                           >  1:2280840/1‑90 (MQ=255)
tGAAGATGGGCGGTGTCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATa                                                                                           >  2:1213945/1‑90 (MQ=255)
  aaGATGGGCGGTGTCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATAcc                                                                                         >  1:390568/1‑90 (MQ=255)
   aGATGGGCGGTGTCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATAccc                                                                                        >  1:1512576/1‑90 (MQ=255)
     aTGGGCGGTGTCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCccg                                                                                      <  1:2522273/90‑1 (MQ=255)
          cGGTGTCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGtt                                                                                 >  2:94171/1‑90 (MQ=255)
             tgtCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGa                                                                              <  1:1847345/90‑1 (MQ=255)
              gtCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGaa                                                                             >  2:1546308/1‑90 (MQ=255)
               tCGATTTACGGCTTAAATTTTCTGGCGATTCCCTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGaaa                                                                            <  1:2699042/90‑1 (MQ=255)
               tCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGaaa                                                                            <  1:1327946/90‑1 (MQ=255)
               tCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGaaa                                                                            >  2:2996656/1‑90 (MQ=255)
               tCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGaaa                                                                            <  1:2901054/90‑1 (MQ=255)
                cGATTTACGGCTTAAATTTTCTGGCGGTTGACTGGGTGATCTCTATGAACTGACGGGAGTTCTGCTGCCCGATACCCTGCCGTTTGAAAc                                                                           >  2:1703271/1‑90 (MQ=255)
                cGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAAc                                                                           >  1:1096399/1‑90 (MQ=255)
                cGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAAc                                                                           >  1:2430536/1‑90 (MQ=255)
                cGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAAc                                                                           <  1:1942255/90‑1 (MQ=255)
                cGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAAc                                                                           >  2:1103485/1‑90 (MQ=255)
                 gATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACg                                                                          >  2:196266/1‑90 (MQ=255)
                  aTTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATCCCCCGCCGTTTGAAACgg                                                                         <  1:2108253/90‑1 (MQ=255)
                     tACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATg                                                                      <  2:1003082/90‑1 (MQ=255)
                      aCGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCTTTCTGCTGCCCGATCCCCCGCCGTTTGAAACGGATgg                                                                     <  2:1925268/90‑1 (MQ=255)
                       cGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGt                                                                    >  1:2795061/1‑90 (MQ=255)
                       cGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGt                                                                    >  2:985470/1‑90 (MQ=255)
                        ggCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTc                                                                   >  1:2564968/1‑90 (MQ=255)
                          cTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCgg                                                                 >  2:155628/1‑90 (MQ=255)
                             aaaTTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTg                                                              >  2:2921676/1‑90 (MQ=255)
                                ttttCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTa                                                           >  2:28723/1‑90 (MQ=255)
                                ttttCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTa                                                           <  1:2899924/90‑1 (MQ=255)
                                ttttCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTa                                                           >  2:454454/1‑90 (MQ=255)
                                 tttCTGGCGATTCACTGTGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAg                                                          <  2:3145058/90‑1 (MQ=255)
                                 tttCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAg                                                          <  1:862638/90‑1 (MQ=255)
                                  ttCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGc                                                         >  1:453973/1‑90 (MQ=255)
                                  ttCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGc                                                         <  1:1250611/90‑1 (MQ=255)
                                  ttCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGc                                                         <  1:2092055/90‑1 (MQ=255)
                                   tCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCg                                                        <  2:2266694/90‑1 (MQ=255)
                                    cTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGa                                                       >  1:1896316/1‑90 (MQ=255)
                                    cTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGa                                                       >  1:2644821/1‑90 (MQ=255)
                                    cTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGa                                                       >  2:2164109/1‑90 (MQ=255)
                                    cTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGa                                                       <  1:1353251/90‑1 (MQ=255)
                                        cGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAAt                                                   <  2:2592872/90‑1 (MQ=255)
                                        cGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAAt                                                   >  2:2681404/1‑90 (MQ=255)
                                        cGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAAt                                                   <  2:2151889/90‑1 (MQ=255)
                                         ggttCACTGGGTGGTCTCTATGAACTGACGGGCGTTCTGCCGCCCGATCCCCCGCCGCTTGAAACGGATGGCCGGCTGGCAGCGAAAATc                                                  <  2:1063313/88‑1 (MQ=255)
                                         gATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGGCGGCTGGTAGCGAAAATc                                                  >  1:2426036/1‑90 (MQ=255)
                                         aaTTCTCTGGGTGACTTCTATGAACTGACGGGCGTTCTGCTCCCCAATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATc                                                  <  2:3188639/89‑1 (MQ=255)
                                          aTTCCCTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCg                                                 <  2:1660236/90‑1 (MQ=255)
                                             cACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGaca                                              <  1:698876/90‑1 (MQ=255)
                                              aCTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGacac                                             >  2:857112/1‑90 (MQ=255)
                                               cTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACt                                            >  1:199046/1‑90 (MQ=255)
                                                 gggTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGa                                          <  2:2966203/90‑1 (MQ=255)
                                                   gTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGaaa                                        <  2:2720830/90‑1 (MQ=255)
                                                   gTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGaaa                                        <  2:3479651/90‑1 (MQ=255)
                                                    tGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGaaaa                                       <  1:2044108/90‑1 (MQ=255)
                                                    tGATCTCTATGAACTGACGGCCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGaaaa                                       <  2:2767193/90‑1 (MQ=255)
                                                     gATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGaaaaa                                      >  2:1151408/1‑90 (MQ=255)
                                                      aTCTCTATGAACTGACGGGCGTTCTGCTGCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAAt                                     <  1:1810299/90‑1 (MQ=255)
                                                      aTCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACGCCGCCGTTTGAAACGGATGGTCGGCCGGTCGCGAAAATCGACACTGAAAAAt                                     >  1:3017094/1‑90 (MQ=255)
                                                           tATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAAtcgtcg                                <  2:310719/90‑1 (MQ=255)
                                                            aTGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAACTCGTCgg                               >  2:1773644/1‑90 (MQ=255)
                                                             tGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGt                              >  2:120332/1‑90 (MQ=255)
                                                             tGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGt                              >  2:2196229/1‑90 (MQ=255)
                                                                aCTGACGGGCGTTGTGCTGCCCCATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCtt                           <  2:796942/90‑1 (MQ=255)
                                                                aCTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCtt                           >  2:3378651/1‑90 (MQ=255)
                                                                 cTGACGGGCGTTCTGCTGCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCttt                          <  2:388758/90‑1 (MQ=255)
                                                                 cTGACGGGCGTTCTGCTGCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCttt                          <  1:1115526/90‑1 (MQ=255)
                                                                  tGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTg                         >  2:1068604/1‑90 (MQ=255)
                                                                   gACGGGCGTTCTGCTGCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGa                        <  2:3064888/90‑1 (MQ=255)
                                                                   gACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGa                        >  2:1556369/1‑90 (MQ=255)
                                                                    aCGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGAt                       >  2:161304/1‑90 (MQ=255)
                                                                     cGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGAtt                      >  1:1459594/1‑90 (MQ=255)
                                                                      gggCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTa                     >  1:3077539/1‑90 (MQ=255)
                                                                      gggCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTa                     >  1:2395701/1‑90 (MQ=255)
                                                                         cGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATcg                  <  2:2409827/90‑1 (MQ=255)
                                                                         cGTTCTGCTCCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATcg                  <  1:818118/90‑1 (MQ=255)
                                                                          gTTCTGCTGCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATcgc                 <  1:609839/90‑1 (MQ=255)
                                                                          gTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATcgc                 >  2:2079737/1‑90 (MQ=255)
                                                                          gTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATcgc                 >  1:1577814/1‑90 (MQ=255)
                                                                           ttCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATcgcg                >  1:2747435/1‑90 (MQ=255)
                                                                            tCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCgg               >  1:1547177/1‑90 (MQ=255)
                                                                             ctgctgCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGt              <  1:1372167/90‑1 (MQ=255)
                                                                             ctgctgCCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGt              <  1:1360376/90‑1 (MQ=255)
                                                                              tgctgcCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGtt             <  1:2181850/90‑1 (MQ=255)
                                                                               gctgcCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGttt            <  1:1622683/90‑1 (MQ=255)
                                                                               gctgcCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGttt            <  1:337558/90‑1 (MQ=255)
                                                                               gctgcCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGttt            <  1:1707527/90‑1 (MQ=255)
                                                                               gctgcCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGttt            <  1:2248995/90‑1 (MQ=255)
                                                                                ctgcCCGATGCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGtttt           <  2:2425132/90‑1 (MQ=255)
                                                                                ctgcCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGtttt           >  2:3227782/1‑90 (MQ=255)
                                                                                 tgcCCGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTa          <  1:1335281/90‑1 (MQ=255)
                                                                                   cccGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAGAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAAt        >  2:1326028/1‑90 (MQ=255)
                                                                                   cccGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATAGTCGGTCTTTGATTATCGCGGTTTTAAt        >  1:1827986/1‑90 (MQ=255)
                                                                                    ccGATTCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAATg       <  2:1922968/90‑1 (MQ=255)
                                                                                     cGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAATgg      <  1:2164144/90‑1 (MQ=255)
                                                                                     cGATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAATgg      <  1:323904/90‑1 (MQ=255)
                                                                                     cGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAATgg      >  2:2185979/1‑90 (MQ=255)
                                                                                      gATCCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAATggg     <  2:1397605/90‑1 (MQ=255)
                                                                                      gATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAATggg     >  2:1382202/1‑90 (MQ=255)
                                                                                       aTACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAATGGGc    <  2:2068776/90‑1 (MQ=255)
                                                                                        tACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAATGGGCg   <  1:1614910/90‑1 (MQ=255)
                                                                                         aCCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAATGGGCGa  <  1:2877020/90‑1 (MQ=255)
                                                                                         |                                                                                         
TGAAGATGGGCGGTGTCGATTTACGGCTTAAATTTTCTGGCGATTCACTGGGTGATCTCTATGAACTGACGGGCGTTCTGCTGCCCGATACCCCGCCGTTTGAAACGGATGGTCGGCTGGTAGCGAAAATCGACACTGAAAAATCGTCGGTCTTTGATTATCGCGGTTTTAATGGGCGA  >  AC_000091/3962879‑3963057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑