Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000914,147,0120AC24.1% 266.1 / 12.0 113G279G (GGT→GGG) tsgApredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (53/32);  new base C (0/27);  total (53/60)
Fisher's exact test for biased strand distribution p-value = 7.67e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 140 total aligned reads displayed.

GCGCTGGAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATGACATCCAGAA  >  AC_000091/4146923‑4147101
                                                                                         |                                                                                         
gcgcTGGAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGa                                                                                           >  1:422080/1‑90 (MQ=255)
 cgcTGGAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGAc                                                                                          >  2:176532/1‑90 (MQ=255)
 cgcTGGAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGAc                                                                                          <  1:1460243/90‑1 (MQ=255)
 cgcTGGAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGAc                                                                                          >  1:886628/1‑90 (MQ=255)
  gcTGGAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGAcc                                                                                         <  2:849166/90‑1 (MQ=255)
  gcTGGAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGAcc                                                                                         >  2:1409330/1‑90 (MQ=255)
   cTGGAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGAcca                                                                                        >  1:2243483/1‑90 (MQ=255)
   cTGGAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGAcca                                                                                        <  2:2648408/90‑1 (MQ=255)
    tGGAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGAccag                                                                                       <  2:2616531/90‑1 (MQ=255)
     ggAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGAccagc                                                                                      <  2:2780104/90‑1 (MQ=255)
      gagaAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGAccagcc                                                                                     <  1:2023078/90‑1 (MQ=255)
        gaagaaGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGAccagccag                                                                                   <  1:1404831/90‑1 (MQ=255)
         aagaagCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGt                                                                                  <  1:688233/90‑1 (MQ=255)
         aagaacCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACTTCAGAATCGCAGCCAGCCCAGCCAGt                                                                                  <  1:3042203/90‑1 (MQ=255)
          agaagCCCAGAGCGAGAATTGACCCCGCCATATGTGCTGGTGTTCCGGTTTTAAAGACGTACATAAGAACCGCAGCCAGACCAGCCAGTa                                                                                 <  2:1822517/90‑1 (MQ=255)
          agaagCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTa                                                                                 <  2:1231551/90‑1 (MQ=255)
            aagCCCAGAGCGAGAATTGCCCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACg                                                                               <  2:3014999/90‑1 (MQ=255)
            aagCCCAGAGCGAGAATTGCCCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACg                                                                               <  2:2031221/90‑1 (MQ=255)
            aagCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACg                                                                               >  1:908744/1‑90 (MQ=255)
            aagCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACg                                                                               >  1:161382/1‑90 (MQ=255)
             agCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAAAATCGCAGCCAGACCAGCCAGTACgg                                                                              <  1:2924186/90‑1 (MQ=255)
              gCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTTTAAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGt                                                                             <  2:1384233/90‑1 (MQ=255)
              gCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGt                                                                             >  1:2079465/1‑90 (MQ=255)
                ccAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAACCGCAGCCAGCCCAGCCAGTACGGTCa                                                                           <  2:1118828/90‑1 (MQ=255)
                  agagCGAGATTTGCCCGCGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTCCATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGa                                                                         <  2:2318781/90‑1 (MQ=255)
                    agCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAAt                                                                       >  2:739972/1‑90 (MQ=255)
                     gCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATg                                                                      >  2:3478191/1‑90 (MQ=255)
                       gagaATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCg                                                                    >  1:2338743/1‑90 (MQ=255)
                        agaATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGt                                                                   >  1:1786830/1‑90 (MQ=255)
                        agaATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGt                                                                   >  2:2394687/1‑90 (MQ=255)
                         gaATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGtt                                                                  <  1:531586/90‑1 (MQ=255)
                          aaTTGCCCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGCCGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTg                                                                 <  2:1023723/90‑1 (MQ=255)
                          aaTTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTg                                                                 >  1:1294609/1‑90 (MQ=255)
                           aTTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGc                                                                >  1:1002964/1‑90 (MQ=255)
                            ttGCCCACGCCATATGTGCTGGTGTTCCGGTTTTAAAGACGTACATCAGAACCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCa                                                               <  1:1419756/90‑1 (MQ=255)
                            ttGCCCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAACCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCa                                                               <  2:2813193/90‑1 (MQ=255)
                             tGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCaa                                                              >  1:269404/1‑90 (MQ=255)
                             tGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCaa                                                              >  2:529145/1‑90 (MQ=255)
                              gcccACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCaaa                                                             <  1:2316027/88‑1 (MQ=255)
                               aCCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAAt                                                            <  1:3285809/90‑1 (MQ=255)
                                ccACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATc                                                           <  1:1614331/90‑1 (MQ=255)
                                ccACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATc                                                           >  1:151939/1‑90 (MQ=255)
                                 cccGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTCCATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCa                                                          <  1:3337703/88‑1 (MQ=255)
                                 cACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCa                                                          >  2:238312/1‑90 (MQ=255)
                                    gCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAaag                                                       >  1:1040177/1‑90 (MQ=255)
                                       atatGTGCTGGTGTTCCGGTTTTAAAGACGTACATCAGAACCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAaagaag                                                    <  2:1741928/90‑1 (MQ=255)
                                       atatGTGCTGGTGTTCCGGTGTTAAAGCCGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAaagaag                                                    <  1:720143/90‑1 (MQ=255)
                                       atatGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAaagaag                                                    <  2:422434/90‑1 (MQ=255)
                                          tgtgCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCga                                                 >  1:1699171/1‑90 (MQ=255)
                                          tgtgCGGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCga                                                 <  1:2347669/90‑1 (MQ=255)
                                           gtgCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCgaa                                                <  2:1039570/90‑1 (MQ=255)
                                           gggCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCgaa                                                <  1:1259259/88‑1 (MQ=255)
                                            tgCTGGGGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCgaag                                               <  2:1336088/90‑1 (MQ=255)
                                             gCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCgaaga                                              >  2:2648496/1‑90 (MQ=255)
                                             gCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCgaaga                                              <  1:2244639/90‑1 (MQ=255)
                                              cTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCgaagaa                                             >  2:3282994/1‑90 (MQ=255)
                                               tGGTGTACCGGTGTTAAAGCCGTCCACCAAATCCGCAGCCAGTCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAAt                                            <  2:2963328/90‑1 (MQ=255)
                                                 ggtttCCGGTGTAAAAGCCGTACACCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATaa                                          <  2:511697/87‑1 (MQ=255)
                                                  tgtTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATaaa                                         >  1:3066908/1‑90 (MQ=255)
                                                   gtTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAg                                        >  2:1693862/1‑90 (MQ=255)
                                                    ttCGGGTGTTAAAGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGc                                       <  1:2642858/90‑1 (MQ=255)
                                                    ttCCGGTGTTAAAGCCGTACATCAGAATGGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGc                                       <  1:2960281/90‑1 (MQ=255)
                                                     tCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCt                                      >  1:2517046/1‑90 (MQ=255)
                                                      ctggTGTAAAAGTCGTAAATCAAAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTg                                     <  2:3002764/88‑1 (MQ=255)
                                                      cgggTGTTAAAGACGTACATCAGAAGGGCACCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATTAAGCTg                                     <  2:405164/88‑1 (MQ=255)
                                                      ccGGTGTTAAAGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTg                                     <  1:997013/90‑1 (MQ=255)
                                                      ccGGTGTTAAAGACGAACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTg                                     <  1:1875300/90‑1 (MQ=255)
                                                       cGGTGTTAAAGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGa                                    <  1:144777/90‑1 (MQ=255)
                                                        ggTGTAAAAGCCGTACATCAAAACCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGaa                                   <  2:1824829/90‑1 (MQ=255)
                                                         gtgtTAAAGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAAc                                  <  2:3121939/90‑1 (MQ=255)
                                                         gtgtTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAAc                                  >  1:1046264/1‑90 (MQ=255)
                                                         gtgtTAAAGACGTACACCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAAc                                  <  2:2148092/90‑1 (MQ=255)
                                                         gtgtAAAAGACGTACATCAAAACCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAAc                                  <  1:1377165/90‑1 (MQ=255)
                                                           gtTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGc                                >  1:1433549/1‑90 (MQ=255)
                                                            ttAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGcc                               >  2:86611/1‑90 (MQ=255)
                                                             tAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGccc                              >  1:3022360/1‑90 (MQ=255)
                                                             tAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGccc                              >  2:296433/1‑90 (MQ=255)
                                                               aaGACGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCcac                            <  1:1458099/90‑1 (MQ=255)
                                                                aGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCcaca                           >  2:2037025/1‑90 (MQ=255)
                                                                aGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCcaca                           >  1:2463171/1‑90 (MQ=255)
                                                                 gccGTACATCAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACAt                          <  1:3292214/88‑1 (MQ=255)
                                                                 gACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCCGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACAt                          >  2:2472410/1‑90 (MQ=255)
                                                                 gACGTACATCAGAACCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACAt                          <  2:2374170/90‑1 (MQ=255)
                                                                      aCATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGa                     <  1:2197540/90‑1 (MQ=255)
                                                                       caccAGAATCGCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGAc                    <  2:3151808/87‑1 (MQ=255)
                                                                       cATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGAc                    <  1:3130746/90‑1 (MQ=255)
                                                                        aTCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGAcc                   >  1:2782477/1‑90 (MQ=255)
                                                                         tCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCa                  >  1:690231/1‑90 (MQ=255)
                                                                         tCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCa                  >  2:1679705/1‑90 (MQ=255)
                                                                               tCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATg            <  1:140564/90‑1 (MQ=255)
                                                                               ccGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATg            <  2:2952997/89‑1 (MQ=255)
                                                                                cGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATGa           <  1:1511022/90‑1 (MQ=255)
                                                                                cGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATGa           >  2:558422/1‑90 (MQ=255)
                                                                                cGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATGa           >  2:2568012/1‑90 (MQ=255)
                                                                                 gCAGCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATGAc          <  2:1757578/90‑1 (MQ=255)
                                                                                   aGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATGACAt        <  2:2274669/90‑1 (MQ=255)
                                                                                    gCCAGCCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATGACATc       <  1:3205161/90‑1 (MQ=255)
                                                                                     ccagcccagcCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATGACATcc      <  2:451928/90‑1 (MQ=255)
                                                                                        gACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATGACATCCAGa   >  2:3309867/1‑90 (MQ=255)
                                                                                         aCCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATGACATCCAGaa  <  2:496963/90‑1 (MQ=255)
                                                                                         |                                                                                         
GCGCTGGAGAAGAAGCCCAGAGCGAGAATTGACCACGCCATATGTGCTGGTGTTCCGGTGTTAAAGACGTACATCAGAATCGCAGCCAGACCAGCCAGTACGGTCAGAATGCGTTGCAAATCAAAGAAGCGAAGAATAAAGCTGAACGCCCACATGCCGACCATGTATGACATCCAGAA  >  AC_000091/4146923‑4147101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑