BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	3,656,842	G→A	I413I (ATC→ATT)	wzyE ←	predicted Wzy protein involved in ECA polysaccharide chain elongation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	3,656,842	0	G	A	100.0%	564.7 / NA	137	I413I (ATC→ATT)	wzyE	predicted Wzy protein involved in ECA polysaccharide chain elongation
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (68/69); total (68/69)

Only 100 of 140 total aligned reads displayed.

TGATTTTGTACGTTTATGAATGAGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACGATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACT  >  AC_000091/3656755‑3656928

   ttttGTACGTTTATGAATGAGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATaaa                                                                                   >  1:3038451/1‑90 (MQ=255)

    tttGTACGTTTATGAATGAGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATaaaa                                                                                  >  2:1012921/1‑90 (MQ=255)

     ttGTACGTTTATGAATGAGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATaaaaa                                                                                 <  2:1188025/90‑1 (MQ=255)

      tGTACGTTTATGAATGAGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATaaaaaa                                                                                <  2:2194578/90‑1 (MQ=255)

            tttATGAATGAGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCAc                                                                          <  1:106313/90‑1 (MQ=255)

            tttATGAATGAGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCAc                                                                          <  2:2530105/90‑1 (MQ=255)

              tATGAATGAGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCAcgc                                                                        >  1:2518368/1‑90 (MQ=255)

               atgaatgaGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCAcgcg                                                                       <  1:1024897/90‑1 (MQ=255)

                tgaatgaGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGt                                                                      >  2:3273409/1‑90 (MQ=255)

                tgaatgaGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGt                                                                      <  2:1647298/90‑1 (MQ=255)

                  aatgaGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTga                                                                    >  1:1257352/1‑90 (MQ=255)

                   atgaGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTgag                                                                   <  2:3082651/90‑1 (MQ=255)

                    tgaGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTgaga                                                                  >  2:2314061/1‑90 (MQ=255)

                      aGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACa                                                                <  2:1278044/90‑1 (MQ=255)

                         ccGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAAc                                                             <  1:1875032/90‑1 (MQ=255)

                         ccGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAAc                                                             <  2:517981/90‑1 (MQ=255)

                         ccGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAAc                                                             <  1:1252895/90‑1 (MQ=255)

                         ccGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAAc                                                             <  1:167041/90‑1 (MQ=255)

                           ggCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGa                                                           <  2:2616216/90‑1 (MQ=255)

                            gcgcTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGaa                                                          >  1:1815296/1‑90 (MQ=255)

                             cgcTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAAt                                                         >  1:2920364/1‑90 (MQ=255)

                              gcTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATGAAAAAGACAACGCGTGAGACAAAGGAATc                                                        >  2:3318683/1‑90 (MQ=255)

                              gcTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATc                                                        <  1:2872734/90‑1 (MQ=255)

                              gcTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATc                                                        <  2:2228780/90‑1 (MQ=255)

                               cTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATcc                                                       >  2:622060/1‑90 (MQ=255)

                                tttCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCa                                                      <  1:655066/90‑1 (MQ=255)

                                  tCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGc                                                    >  1:2042056/1‑90 (MQ=255)

                                  tCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGc                                                    >  1:1850808/1‑90 (MQ=255)

                                  tCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGc                                                    >  1:2874208/1‑90 (MQ=255)

                                   cAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGcc                                                   <  1:2280945/90‑1 (MQ=255)

                                   cAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGcc                                                   <  1:1777203/90‑1 (MQ=255)

                                     aaaaGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCt                                                 <  1:3426452/90‑1 (MQ=255)

                                      aaaGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCtt                                                >  1:320037/1‑90 (MQ=255)

                                       aaGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTc                                               <  1:427256/90‑1 (MQ=255)

                                       aaGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTc                                               >  2:765808/1‑90 (MQ=255)

                                         gCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCAc                                             <  2:2853680/90‑1 (MQ=255)

                                          ccAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCAcg                                            >  1:2498249/1‑90 (MQ=255)

                                           cAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCAcgc                                           >  2:2170258/1‑90 (MQ=255)

                                           cAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCAcgc                                           >  2:2025875/1‑90 (MQ=255)

                                            aGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCAcgcg                                          >  1:1945253/1‑90 (MQ=255)

                                            aGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCAcgcg                                          >  1:2524869/1‑90 (MQ=255)

                                             gttcaacaGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACgcgc                                         <  1:1208922/87‑1 (MQ=255)

                                             gTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACgcgc                                         >  1:3089623/1‑90 (MQ=255)

                                             gTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGACACAAACGAATCCAGCCCTTCACgcgc                                         >  1:431072/1‑90 (MQ=255)

                                               acaacaGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCa                                       <  2:2851354/90‑1 (MQ=255)

                                               acaacaGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCa                                       >  2:2012879/1‑90 (MQ=255)

                                                caacaGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAg                                      <  2:2548606/90‑1 (MQ=255)

                                                 aacaGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAga                                     >  2:2399958/1‑89 (MQ=255)

                                                 aacaGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGc                                     >  1:2899357/1‑90 (MQ=255)

                                                 aacaGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGc                                     >  2:2988415/1‑90 (MQ=255)

                                                  acaGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCa                                    >  2:1249272/1‑90 (MQ=255)

                                                   caGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCAc                                   <  1:420818/90‑1 (MQ=255)

                                                   caGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCAc                                   <  1:2308946/90‑1 (MQ=255)

                                                   caGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCAc                                   <  1:948147/90‑1 (MQ=255)

                                                   caGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCAc                                   >  2:1944588/1‑90 (MQ=255)

                                                    aGTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACg                                  >  1:1401947/1‑90 (MQ=255)

                                                     gTTTTGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGa                                 <  1:1975396/90‑1 (MQ=255)

                                                       tttGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATc                               <  1:2470422/90‑1 (MQ=255)

                                                       tttGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGCGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGAta                               >  2:1601782/1‑89 (MQ=255)

                                                         tGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCat                             >  1:2478274/1‑90 (MQ=255)

                                                         tGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCat                             <  1:691873/90‑1 (MQ=255)

                                                         tGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCat                             >  1:1713734/1‑90 (MQ=255)

                                                         tGCGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCat                             >  2:2817154/1‑90 (MQ=255)

                                                           cGATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCatat                           >  1:3132559/1‑90 (MQ=255)

                                                            gATCATCAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATAtt                          >  1:2538879/1‑90 (MQ=255)

                                                             atcatcAGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTg                         >  1:1175214/1‑90 (MQ=255)

                                                              tcatcaGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGa                        <  1:1615055/90‑1 (MQ=255)

                                                               catcaGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGaa                       <  1:2391916/90‑1 (MQ=255)

                                                               catcaGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGaa                       <  2:13428/90‑1 (MQ=255)

                                                                atcaGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAg                      <  1:3037353/90‑1 (MQ=255)

                                                                 tcaGACATGCGCCGAAGGCCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGa                     >  2:1620349/1‑90 (MQ=255)

                                                                 tcaGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGa                     >  1:2701450/1‑90 (MQ=255)

                                                                  caGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGAt                    <  1:2417871/90‑1 (MQ=255)

                                                                  caGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGAt                    >  2:726416/1‑90 (MQ=255)

                                                                  caGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGAt                    <  1:318378/90‑1 (MQ=255)

                                                                   aGACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATc                   >  2:166293/1‑90 (MQ=255)

                                                                    gACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCg                  <  1:2624633/90‑1 (MQ=255)

                                                                    gACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCg                  <  2:1614698/90‑1 (MQ=255)

                                                                    gACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCg                  >  1:904942/1‑90 (MQ=255)

                                                                    gACATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCg                  <  1:347273/90‑1 (MQ=255)

                                                                      cATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGATACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGcc                <  2:65241/90‑1 (MQ=255)

                                                                      cATGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGcc                <  1:2425876/90‑1 (MQ=255)

                                                                        tGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGcccc              >  1:3147474/1‑90 (MQ=255)

                                                                        tGCGCCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGcccc              >  2:1049537/1‑90 (MQ=255)

                                                                        tGCGCCGAAGACCACAATAAAAAAGACCACACGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGccca              >  2:703683/1‑89 (MQ=255)

                                                                         gcgcCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCa             <  1:3233263/90‑1 (MQ=255)

                                                                          cgcCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa            >  1:2585934/1‑90 (MQ=255)

                                                                          cgcCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa            <  1:2978639/90‑1 (MQ=255)

                                                                           gcCGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaaa           <  2:2267954/90‑1 (MQ=255)

                                                                            ccGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAg          >  1:1068326/1‑90 (MQ=255)

                                                                             cGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGc         <  2:2663259/90‑1 (MQ=255)

                                                                             cGAAGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGc         >  2:1439391/1‑90 (MQ=255)

                                                                               aaGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAg       <  1:2553331/90‑1 (MQ=255)

                                                                                aGACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGa      <  1:912372/90‑1 (MQ=255)

                                                                                 gACCACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGaa     >  2:2229288/1‑90 (MQ=255)

                                                                                   ccACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAAc   <  2:3391345/90‑1 (MQ=255)

                                                                                   ccACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAAc   <  1:1739449/90‑1 (MQ=255)

                                                                                   ccACAATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAAc   <  2:2507558/90‑1 (MQ=255)

                                                                                    cacaATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACt  >  2:3476871/1‑90 (MQ=255)

                                                                                    cacaATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACt  <  1:1816655/90‑1 (MQ=255)

TGATTTTGTACGTTTATGAATGAGTCCGGCGCTTTCAAAAAGCCAGTACAACAGTTTTGCGATCATCAGACATGCGCCGAAGACCACGATAAAAAAGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACT  >  AC_000091/3656755‑3656928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`