BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	3,711,417	G→A	A124T (GCG→ACG)	gidA →	glucose‑inhibited cell‑division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	3,711,417	0	G	A	100.0%	511.9 / NA	131	A124T (GCG→ACG)	gidA	glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (55/76); total (55/76)

Only 100 of 133 total aligned reads displayed.

CCGAGCTCAGGCGGATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGGCGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGTGCCAAAGCCGTCGTG  >  AC_000091/3711332‑3711503

ccGAGCTCAGGCGGATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGt                                                                                    <  1:1067872/90‑1 (MQ=255)

ccGAGCTCAGGCGGATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGt                                                                                    <  1:2476439/90‑1 (MQ=255)

 cGAGCTCAGGCGGATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGtt                                                                                   >  1:785302/1‑90 (MQ=255)

  gAGCTCAGGCGGATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTg                                                                                  >  1:315884/1‑90 (MQ=255)

    gCTCAGGCGGATCGTGTGCTCTTCCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGaa                                                                                <  2:49576/90‑1 (MQ=255)

    gCTCAGGCGGATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGaa                                                                                <  2:520241/90‑1 (MQ=255)

     cTCAGGCGGATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAg                                                                               <  1:2470159/90‑1 (MQ=255)

      tCAGGCGGATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGa                                                                              <  2:1449551/90‑1 (MQ=255)

       cAGGCGGATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGAt                                                                             <  1:332764/90‑1 (MQ=255)

           cGGATCGTGTGCTCTCCCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTa                                                                         <  1:2644469/90‑1 (MQ=255)

            ggATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGCTCTTCCAGCAGACGGTTGAAGATCTTAt                                                                        >  2:1424264/1‑90 (MQ=255)

             gATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTAtt                                                                       <  1:2274584/90‑1 (MQ=255)

             gATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTAtt                                                                       <  1:1872835/90‑1 (MQ=255)

             gATCGGCTGCTCCGCCGTCAGGCGGTACGCACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTAtt                                                                       <  1:2571900/90‑1 (MQ=255)

              aTCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTg                                                                      <  1:639768/90‑1 (MQ=255)

              aTCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGATGAAGATCTTATTg                                                                      >  1:484592/1‑90 (MQ=255)

               tCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGt                                                                     <  2:696954/90‑1 (MQ=255)

               tCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGt                                                                     >  1:3047243/1‑90 (MQ=255)

                cGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTc                                                                    <  1:1188536/90‑1 (MQ=255)

                 ttgtgCTCTTCCGCCAGGCGGTAGGTACGGCGCTGGATAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCg                                                                   <  2:1465726/89‑1 (MQ=255)

                 gtgtGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACTAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCg                                                                   <  2:1293728/90‑1 (MQ=255)

                 gtgtGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCg                                                                   >  2:2707828/1‑90 (MQ=255)

                 gtgtGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCg                                                                   <  1:1196409/90‑1 (MQ=255)

                 gtgtGCTATACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCg                                                                   <  1:1207770/90‑1 (MQ=255)

                   gtgCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGaa                                                                 <  2:678066/90‑1 (MQ=255)

                   gtgCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGaa                                                                 <  2:155485/90‑1 (MQ=255)

                   gtgCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGaa                                                                 <  1:1959824/90‑1 (MQ=255)

                     gCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGaaaa                                                               >  1:917057/1‑90 (MQ=255)

                     gCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGaaaa                                                               <  2:2580891/90‑1 (MQ=255)

                      ctctACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAAc                                                              <  1:2322509/90‑1 (MQ=255)

                      ctctACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAAc                                                              <  1:78761/90‑1 (MQ=255)

                      ctctACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAAc                                                              <  1:568213/90‑1 (MQ=255)

                      ctctACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAAc                                                              <  1:2824491/90‑1 (MQ=255)

                       tctACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAATCg                                                             <  2:724321/90‑1 (MQ=255)

                       tctACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACg                                                             >  2:2596225/1‑90 (MQ=255)

                       tctACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACg                                                             >  1:993625/1‑90 (MQ=255)

                         tACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGac                                                           >  1:1104802/1‑89 (MQ=255)

                         tACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGAt                                                           >  2:2476433/1‑90 (MQ=255)

                          aCCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATc                                                          <  2:2338342/90‑1 (MQ=255)

                          aCCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATc                                                          <  2:1273399/90‑1 (MQ=255)

                          aCCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATc                                                          <  1:3423445/90‑1 (MQ=255)

                           ccGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATcg                                                         >  1:90566/1‑90 (MQ=255)

                           ccGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATcg                                                         >  1:121590/1‑90 (MQ=255)

                            cGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATcgc                                                        <  1:2721837/90‑1 (MQ=255)

                            cGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATcgc                                                        <  2:1953501/90‑1 (MQ=255)

                            cGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATcgc                                                        <  1:859371/90‑1 (MQ=255)

                              tCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGt                                                      >  1:1742108/1‑90 (MQ=255)

                               cAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTg                                                     >  2:2085572/1‑90 (MQ=255)

                               cAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTg                                                     <  2:2938377/90‑1 (MQ=255)

                                aGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTgg                                                    >  2:588926/1‑90 (MQ=255)

                                aGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTgg                                                    <  1:787140/90‑1 (MQ=255)

                                aGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTgg                                                    <  1:370823/90‑1 (MQ=255)

                                aGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTgg                                                    >  1:344764/1‑90 (MQ=255)

                                aGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTgg                                                    <  1:1583174/90‑1 (MQ=255)

                                 ggCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGt                                                   >  2:651873/1‑90 (MQ=255)

                                 ggCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGt                                                   <  1:332854/90‑1 (MQ=255)

                                  gCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTc                                                  <  1:533548/90‑1 (MQ=255)

                                  gCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTc                                                  <  1:1878849/90‑1 (MQ=255)

                                   cGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCg                                                 >  1:45414/1‑90 (MQ=255)

                                   cGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCg                                                 >  2:1173513/1‑90 (MQ=255)

                                    ggTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCgg                                                >  2:2242521/1‑90 (MQ=255)

                                    ggTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCgg                                                >  2:450776/1‑90 (MQ=255)

                                       acgtacgGCGCTGGAGAACTAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGc                                             <  2:2738657/90‑1 (MQ=255)

                                        cgtacgGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCt                                            <  1:3118900/90‑1 (MQ=255)

                                         gtacgGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTg                                           <  1:2913480/90‑1 (MQ=255)

                                         gtacgGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTg                                           >  2:649330/1‑90 (MQ=255)

                                         gtacgGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGGGCTg                                           >  2:353719/1‑90 (MQ=255)

                                         gtacgGCGCTGCAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTg                                           >  2:2519024/1‑90 (MQ=255)

                                           acgGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGtt                                         >  2:2181225/1‑90 (MQ=255)

                                            cgGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTa                                        >  2:2762988/1‑90 (MQ=255)

                                             ggCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTAc                                       >  1:1730443/1‑90 (MQ=255)

                                               cgcTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTAccc                                     >  1:1068515/1‑90 (MQ=255)

                                               cgcTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTAccc                                     >  1:207912/1‑90 (MQ=255)

                                                   ggAGAACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAAt                                 <  2:3227911/90‑1 (MQ=255)

                                                    gagaACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATg                                <  1:2144537/90‑1 (MQ=255)

                                                      gaACCAACCGAACCTTATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTTCCCAAATggg                              <  1:1983671/90‑1 (MQ=255)

                                                      gaACCAACCGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATggg                              >  1:2206893/1‑90 (MQ=255)

                                                       aaccaaccGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGa                             <  1:2476353/90‑1 (MQ=255)

                                                       aaccaaccGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATCGGa                             <  2:2210433/90‑1 (MQ=255)

                                                           aaccGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGa                         >  2:2338592/1‑90 (MQ=255)

                                                           aaccGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGa                         <  2:1657974/90‑1 (MQ=255)

                                                            accGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGaa                        >  2:2305658/1‑90 (MQ=255)

                                                             ccGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAg                       <  1:2032527/90‑1 (MQ=255)

                                                              cGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCTGTGCTGTTACCCAAATGGGACTGAAGt                      <  2:1344122/90‑1 (MQ=255)

                                                              cGAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGGCTGAAGt                      >  2:1078891/1‑90 (MQ=255)

                                                               gAACCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGtt                     <  2:3350540/90‑1 (MQ=255)

                                                                aaCCTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTc                    >  1:3381536/1‑90 (MQ=255)

                                                                  ccTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCg                  <  2:2345526/90‑1 (MQ=255)

                                                                  ccTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCg                  <  1:1297121/90‑1 (MQ=255)

                                                                  ccTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCg                  >  1:2813353/1‑90 (MQ=255)

                                                                   cTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGt                 >  1:1421156/1‑90 (MQ=255)

                                                                   cTGATGATCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGCTCCGt                 >  2:3349554/1‑90 (MQ=255)

                                                                     gatgatCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGTGc               >  1:1772991/1‑90 (MQ=255)

                                                                        gatCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGTGCCaa            <  2:1676581/90‑1 (MQ=255)

                                                                          tCTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGTGCCAAAg          <  2:3047243/90‑1 (MQ=255)

                                                                           cTTCCAGCAGACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGTGCCAAAGc         <  1:2259881/90‑1 (MQ=255)

                                                                               cagcagACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGTGCCAAAGCcgtc     <  1:1908810/90‑1 (MQ=255)

                                                                                agcagACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGTGCCAAAGCcgtcg    >  2:2771130/1‑90 (MQ=255)

                                                                                  cagACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGTGCCAAAGCCGTCGTg  >  1:604999/1‑90 (MQ=255)

                                                                                  cagACGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGTGCCAAAGCCGTCGTg  >  2:3099578/1‑90 (MQ=255)

CCGAGCTCAGGCGGATCGTGTGCTCTACCGTCAGGCGGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGGCGGTTGAAGATCTTATTGTCGAAAACGATCGCGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGTGCCAAAGCCGTCGTG  >  AC_000091/3711332‑3711503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`