Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	3,725,678	G→A	A175T (GCG→ACG)	pstS →	phosphate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	3,725,678	0	G	A	100.0%	366.7 / NA	90	A175T (GCG→ACG)	pstS	phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/47);  total (43/47)

CCGGGTCTGAAACTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGGCGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCGGTAAAGGTAACGAC  >  AC_000091/3725591‑3725764                                                                                        |                                                                                       ccGGGTCTGAAACTGCCTTCACAAAATATTGCTGTAGTACGCCGCGCAGATGCCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACg                                                                                      <  1:2436420/90‑1 (MQ=255)   gggTCTGAAACTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGaa                                                                                    >  1:935684/1‑90 (MQ=255)   gggTCTGAAACTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGaa                                                                                    <  1:2494464/90‑1 (MQ=255)      tCTGAAACTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGt                                                                                 <  1:1333704/90‑1 (MQ=255)      tCTGAAACTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGt                                                                                 >  1:2444911/1‑90 (MQ=255)      tCTGAAACTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGt                                                                                 >  1:2905889/1‑90 (MQ=255)        tGAAACTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGa                                                                               >  1:2237161/1‑90 (MQ=255)        tGAAACTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGa                                                                               >  2:1006203/1‑90 (MQ=255)         gAAACTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGaa                                                                              <  2:2591564/90‑1 (MQ=255)         gAAACTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGaa                                                                              >  2:2979992/1‑90 (MQ=255)          aaaCTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAAc                                                                             >  1:488359/1‑90 (MQ=255)          aaaCTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAAc                                                                             >  1:2932720/1‑90 (MQ=255)          aaaCTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAAc                                                                             >  1:1409152/1‑90 (MQ=255)          aaaCTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAAc                                                                             <  2:1779422/90‑1 (MQ=255)           aaCTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACg                                                                            >  2:2528652/1‑90 (MQ=255)             cTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGaa                                                                          <  1:2759476/90‑1 (MQ=255)             cTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGaa                                                                          >  2:2188068/1‑90 (MQ=255)             cTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGaa                                                                          >  1:2237396/1‑90 (MQ=255)              tGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAag                                                                         <  1:3409833/90‑1 (MQ=255)              tGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAag                                                                         >  2:2042546/1‑90 (MQ=255)                ccTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAagag                                                                       >  1:1003236/1‑90 (MQ=255)                 cTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGt                                                                      <  2:2968110/90‑1 (MQ=255)                  ttCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCCCCAGCTACCTGACGAAAGTGAACGAAGAGTg                                                                     <  2:3188774/90‑1 (MQ=255)                  ttCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTg                                                                     <  2:1348712/90‑1 (MQ=255)                  ttCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTg                                                                     >  1:2565088/1‑90 (MQ=255)                  ttCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTg                                                                     <  1:2020026/90‑1 (MQ=255)                  ttCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTg                                                                     >  1:444688/1‑90 (MQ=255)                  ttCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTg                                                                     >  2:2194574/1‑90 (MQ=255)                   tCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTgg                                                                    <  2:1630083/90‑1 (MQ=255)                   tCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTgg                                                                    >  2:3094348/1‑90 (MQ=255)                   tCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTAAACGAAGAGTgg                                                                    >  2:2809580/1‑90 (MQ=255)                     acaAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGaa                                                                  <  2:696603/90‑1 (MQ=255)                      caAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGaaa                                                                 >  1:1028071/1‑90 (MQ=255)                       aaaaCATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGaaaa                                                                >  1:2797902/1‑90 (MQ=255)                        aaaCATTGCTGTAGTACGCCACGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGaaaaa                                                               <  1:411467/90‑1 (MQ=255)                         aaCATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAaac                                                              <  2:231719/90‑1 (MQ=255)                         aaCATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAaac                                                              >  2:806069/1‑90 (MQ=255)                           cATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAaacaa                                                            >  2:1478345/1‑90 (MQ=255)                            aTTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAaacaac                                                           <  2:3129948/90‑1 (MQ=255)                            aTTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAaacaac                                                           >  2:2745563/1‑90 (MQ=255)                            aTTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAaacaac                                                           <  1:1199367/90‑1 (MQ=255)                             ttGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACg                                                          <  2:3433706/90‑1 (MQ=255)                              tGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGt                                                         <  2:1856779/90‑1 (MQ=255)                               gCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTTGAAAAACAACGtt                                                        <  2:2600969/90‑1 (MQ=255)                                cTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTg                                                       <  2:1438319/90‑1 (MQ=255)                                   tagtaCGCGGCGCAGATGGCTCCGGGACTTCCTTCGTCTTTACCAGCTACCTGACGAAAGGGAACGAAGAGTGGAAAAACAACGTTggaa                                                    >  2:1785727/1‑88 (MQ=255)                                   tagtaCGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTa                                                    <  2:380173/90‑1 (MQ=255)                                   tagtaCGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTa                                                    <  1:2149755/90‑1 (MQ=255)                                    agtaCGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTAc                                                   <  2:2249677/90‑1 (MQ=255)                                     gtaCGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACt                                                  <  1:113612/90‑1 (MQ=255)                                     gtaCGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACt                                                  >  2:3083610/1‑90 (MQ=255)                                      taCGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTg                                                 <  1:2469341/90‑1 (MQ=255)                                      taCGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTg                                                 <  2:1017707/90‑1 (MQ=255)                                         gccgcGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGct                                              <  2:928535/90‑1 (MQ=255)                                         gccgcGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGct                                              <  2:3148491/90‑1 (MQ=255)                                          ccgcGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTCCCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGctc                                             <  2:3298314/90‑1 (MQ=255)                                           cgcgCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGctct                                            >  1:3140525/1‑90 (MQ=255)                                              gcAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTAcc                                         >  2:1792710/1‑90 (MQ=255)                                              gcAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTAcc                                         <  2:3327147/90‑1 (MQ=255)                                               cAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCg                                        <  2:3306313/90‑1 (MQ=255)                                               cAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCg                                        >  2:2121989/1‑90 (MQ=255)                                                 gATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTa                                      >  2:1798313/1‑90 (MQ=255)                                                  aTGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTaa                                     >  2:1330911/1‑90 (MQ=255)                                                   tGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTaaa                                    <  2:949753/90‑1 (MQ=255)                                                     gCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAAt                                  >  1:2241426/1‑90 (MQ=255)                                                           ggACTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGa                            <  1:2141725/90‑1 (MQ=255)                                                             aCTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATc                          <  1:2342360/90‑1 (MQ=255)                                                             aCTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATc                          <  1:1764222/90‑1 (MQ=255)                                                             aCTTCCTTCGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATc                          <  1:1916630/90‑1 (MQ=255)                                                              cttccttcGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCg                         >  1:262850/1‑90 (MQ=255)                                                               ttccttcGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCgg                        <  2:1396391/90‑1 (MQ=255)                                                               ttccttcGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCgg                        <  1:699804/90‑1 (MQ=255)                                                               ttccttcGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGGGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCgg                        >  2:1197960/1‑90 (MQ=255)                                                                tccttcGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCggc                       >  1:3311157/1‑89 (MQ=255)                                                                  cttcGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCt                     <  2:1887558/90‑1 (MQ=255)                                                                  cttcGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCt                     >  2:2108628/1‑90 (MQ=255)                                                                  cttcGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCt                     <  1:25246/90‑1 (MQ=255)                                                                   ttcGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTg                    <  1:1658783/90‑1 (MQ=255)                                                                    tcGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTgg                   >  1:2295888/1‑90 (MQ=255)                                                                     cGTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTggg                  <  2:901517/90‑1 (MQ=255)                                                                      gTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGc                 >  1:2133970/1‑90 (MQ=255)                                                                      gTCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCCCTACCGTAAAATGGCCGATCGGTCTGGGc                 <  1:2104679/90‑1 (MQ=255)                                                                       tCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCg                <  1:1959772/90‑1 (MQ=255)                                                                       tCTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCg                >  1:1789394/1‑90 (MQ=255)                                                                        cTTCACCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCgg               >  2:3431484/1‑90 (MQ=255)                                                                            aCCAGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCGGTaaa           <  2:1877404/90‑1 (MQ=255)                                                                              cAGCTAACTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTAGTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCGGTAAAgg         <  2:1188034/90‑1 (MQ=255)                                                                               aGCTACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCGGTAAAGGt        >  2:2448202/1‑90 (MQ=255)                                                                                  tACCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCGGTAAAGGTAac     >  1:991150/1‑90 (MQ=255)                                                                                   aCCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCGGTAAAGGTAacg    >  1:3020588/1‑90 (MQ=255)                                                                                   aCCTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCGGTAAAGGTAacg    <  2:643725/90‑1 (MQ=255)                                                                                     cTGACGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCGGTAAAGGTAacgac  >  2:2730914/1‑90 (MQ=255)                                                                                        |                                                                                       CCGGGTCTGAAACTGCCTTCACAAAACATTGCTGTAGTACGCCGCGCAGATGGCTCCGGGACTTCCTTCGTCTTCACCAGCTACCTGGCGAAAGTGAACGAAGAGTGGAAAAACAACGTTGGTACTGGCTCTACCGTAAAATGGCCGATCGGTCTGGGCGGTAAAGGTAACGAC  >  AC_000091/3725591‑3725764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑