Predicted mutation
evidence seq id position mutation annotation gene description
RA AC_000091 4,144,590 A→G F606L (TTT→CTT)  nirB ← nitrite reductase, large subunit, NAD(P)H‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000914,144,5900AG100.0% 493.3 / NA 126F606L (TTT→CTT) nirBnitrite reductase, large subunit, NAD(P)H‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (54/72);  total (54/72)

Only 100 of 130 total aligned reads displayed.

GTGCTTTCGCATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAACATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGCCCTTCCGGGG  >  AC_000091/4144503‑4144676
                                                                                       |                                                                                      
 tGCTTTCGCATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCAt                                                                                     >  2:183513/1‑90 (MQ=255)
 tGCTTTCGCATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCAt                                                                                     <  2:436436/90‑1 (MQ=255)
 tGCTTTCGCATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCAt                                                                                     <  1:385929/90‑1 (MQ=255)
  gCTTTCGCATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATc                                                                                    <  2:1429438/90‑1 (MQ=255)
   cTTTCGCATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCg                                                                                   >  1:92490/1‑90 (MQ=255)
     ttCGCATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGcc                                                                                 >  1:3367146/1‑90 (MQ=255)
       cGCATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAg                                                                               <  1:3209089/90‑1 (MQ=255)
        gCATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGa                                                                              >  1:930030/1‑90 (MQ=255)
         cATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGAc                                                                             <  2:723363/90‑1 (MQ=255)
         cATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGAc                                                                             <  1:2396633/90‑1 (MQ=255)
         cATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGAc                                                                             >  1:235251/1‑90 (MQ=255)
          aTAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACg                                                                            >  1:2156375/1‑90 (MQ=255)
          aTAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACg                                                                            <  1:67807/90‑1 (MQ=255)
          aTAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACg                                                                            >  1:2572408/1‑90 (MQ=255)
          aTAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACg                                                                            >  2:2549802/1‑90 (MQ=255)
             ggCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTg                                                                         <  1:3289154/90‑1 (MQ=255)
              gCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTgg                                                                        <  2:1729507/90‑1 (MQ=255)
              gCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTgg                                                                        <  1:85343/90‑1 (MQ=255)
               cATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTggg                                                                       <  2:1277581/90‑1 (MQ=255)
               cATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCAAAGCATCGCCAGACGCTggg                                                                       >  1:376123/1‑90 (MQ=255)
                aTGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGa                                                                      <  1:2183892/90‑1 (MQ=255)
                aTGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGa                                                                      >  1:3178436/1‑90 (MQ=255)
                aTGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGa                                                                      <  2:2339800/90‑1 (MQ=255)
                 tGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAg                                                                     <  1:2390064/90‑1 (MQ=255)
                  gACCGGATTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGc                                                                    >  2:3451541/1‑90 (MQ=255)
                   aCCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGcc                                                                   <  2:2013994/90‑1 (MQ=255)
                   aCCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGcc                                                                   <  2:774366/90‑1 (MQ=255)
                   aCCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGcc                                                                   <  1:1116198/90‑1 (MQ=255)
                    ccGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCa                                                                  <  1:2127924/90‑1 (MQ=255)
                    ccGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCa                                                                  <  1:2134211/90‑1 (MQ=255)
                     cGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAg                                                                 >  1:2980915/1‑90 (MQ=255)
                      ggTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAgc                                                                >  2:3146587/1‑89 (MQ=255)
                      ggTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGt                                                                <  1:172215/90‑1 (MQ=255)
                      ggTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGt                                                                <  2:382658/90‑1 (MQ=255)
                       gTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTg                                                               <  1:2284575/90‑1 (MQ=255)
                        tttCGAAGCAGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGa                                                              <  1:472286/90‑1 (MQ=255)
                         ttCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGAt                                                             >  2:1372095/1‑90 (MQ=255)
                         ttCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGAt                                                             <  1:803307/90‑1 (MQ=255)
                          tCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATc                                                            <  1:585941/90‑1 (MQ=255)
                           cGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCt                                                           <  2:1200060/90‑1 (MQ=255)
                            gAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCtt                                                          >  1:2305561/1‑90 (MQ=255)
                            gAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCtt                                                          <  2:1008251/90‑1 (MQ=255)
                            gAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCtt                                                          >  2:3336470/1‑90 (MQ=255)
                            gAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGATGGGAGCCAGTGATCtt                                                          >  1:1336276/1‑90 (MQ=255)
                             aaGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTg                                                         >  2:3441554/1‑90 (MQ=255)
                             aaGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTg                                                         <  1:3067835/90‑1 (MQ=255)
                               gCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGgt                                                       <  1:1663075/90‑1 (MQ=255)
                                ccGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGgtg                                                      <  1:3366061/90‑1 (MQ=255)
                                  ggCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTa                                                    <  1:1678683/90‑1 (MQ=255)
                                  ggCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTa                                                    <  1:537532/90‑1 (MQ=255)
                                   gCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTag                                                   >  2:66255/1‑90 (MQ=255)
                                    cTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTaga                                                  <  1:823620/90‑1 (MQ=255)
                                    cTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTaga                                                  <  2:2137895/90‑1 (MQ=255)
                                    cTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGCGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTaga                                                  >  2:108111/1‑90 (MQ=255)
                                     ttCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTagag                                                 <  2:2474747/90‑1 (MQ=255)
                                     ttCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTagag                                                 >  1:3117246/1‑90 (MQ=255)
                                       cAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGAt                                               <  2:419471/90‑1 (MQ=255)
                                       cAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGAt                                               <  2:475569/90‑1 (MQ=255)
                                       cAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGAt                                               <  2:1391597/90‑1 (MQ=255)
                                       cAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGAt                                               <  1:3028030/90‑1 (MQ=255)
                                        aaTCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGAtt                                              <  1:371536/90‑1 (MQ=255)
                                          tCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTaa                                            <  2:299698/90‑1 (MQ=255)
                                           cAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTaaa                                           >  2:1946731/1‑90 (MQ=255)
                                                gACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCAc                                      <  2:805360/90‑1 (MQ=255)
                                                gACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCAc                                      <  2:1540305/90‑1 (MQ=255)
                                                gACGCCAGATCTCCGGCAGACCGTCTTTCTGTGCGCCAAGCATCGCCAAACGCTGGGAGCCAGTGACCTAGGTGTAGAGATTAAATTCAc                                      >  2:2939335/1‑90 (MQ=255)
                                                 aCGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCAcg                                     <  1:1162637/90‑1 (MQ=255)
                                                  cGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCAcgc                                    <  2:2613237/90‑1 (MQ=255)
                                                   gCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCAcgcg                                   >  1:2919317/1‑90 (MQ=255)
                                                     cAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCAcgcgcg                                 >  2:1721539/1‑90 (MQ=255)
                                                     cAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCAcgcgcg                                 <  2:3431325/90‑1 (MQ=255)
                                                      aGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGa                                >  2:769675/1‑90 (MQ=255)
                                                      aGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGCGATCTTGGTGTAGAGATTAAATTCACGCGCGa                                >  2:2622069/1‑90 (MQ=255)
                                                        aTCTCCGGCAGATCGTGTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATa                              <  2:2187448/90‑1 (MQ=255)
                                                        aTCTCCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATa                              >  1:1342975/1‑90 (MQ=255)
                                                         tctcCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATAc                             <  2:2135956/90‑1 (MQ=255)
                                                          ctcCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACg                            >  2:1669422/1‑90 (MQ=255)
                                                          ctcCGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACg                            >  1:943545/1‑90 (MQ=255)
                                                             cGGCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGAcc                         >  1:2685827/1‑90 (MQ=255)
                                                              ggCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCt                        >  1:2369021/1‑90 (MQ=255)
                                                              ggCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCt                        <  1:2857326/90‑1 (MQ=255)
                                                               gCAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTa                       <  2:2991335/90‑1 (MQ=255)
                                                                cAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTAc                      <  2:649111/90‑1 (MQ=255)
                                                                cAGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTAc                      >  2:1295428/1‑90 (MQ=255)
                                                                 aGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTAcc                     >  1:1294582/1‑90 (MQ=255)
                                                                 aGATCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTAcc                     >  1:1471439/1‑90 (MQ=255)
                                                                   aTCGTCTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGc                   >  1:1759517/1‑90 (MQ=255)
                                                                        cTTTCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCa              <  1:1005386/90‑1 (MQ=255)
                                                                          ttCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGc            <  1:1020278/90‑1 (MQ=255)
                                                                          ttCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTAGGGGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGc            >  2:63476/1‑90 (MQ=255)
                                                                           tttgtgCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGcc           <  2:10585/88‑1 (MQ=255)
                                                                           tCTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGCTTAAATTCACGCGCGATACGACCTACCGCCATCAGcc           <  1:1901311/90‑1 (MQ=255)
                                                                            cTGTGCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGccc          <  2:1086544/90‑1 (MQ=255)
                                                                              gtgCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGCCCtt        <  1:2023533/90‑1 (MQ=255)
                                                                               tgCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGCCCTTc       >  1:2429835/1‑90 (MQ=255)
                                                                               tgCGCCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGCCCTTc       >  1:3070904/1‑90 (MQ=255)
                                                                                gcgcCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGCCCTTcc      <  2:2810420/90‑1 (MQ=255)
                                                                                 cgcCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGCCCTTCCg     >  2:3201189/1‑90 (MQ=255)
                                                                                  gcCAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGCCCTTCCgg    <  1:2760307/90‑1 (MQ=255)
                                                                                    cAAGCATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGCCCTTCCgggg  <  2:2290090/90‑1 (MQ=255)
                                                                                       |                                                                                      
GTGCTTTCGCATAGGCATGACCGGTTTCGAAGCCGGCTTCAATCAGCTGACGCCAGATCTCCGGCAGATCGTCTTTCTGTGCGCCAAACATCGCCAGACGCTGGGAGCCAGTGATCTTGGTGTAGAGATTAAATTCACGCGCGATACGACCTACCGCCATCAGCCCTTCCGGGG  >  AC_000091/4144503‑4144676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑