BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	4,245,382	C→T	A155T (GCT→ACT)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	4,245,382	0	C	T	100.0%	644.1 / NA	164	A155T (GCT→ACT)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (93/71); total (93/71)

Only 100 of 166 total aligned reads displayed.

AATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGA  >  AC_000091/4245295‑4245468

aaTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCa                                                                                      >  1:3114548/1‑90 (MQ=255)

  tAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGt                                                                                    >  1:1837735/1‑90 (MQ=255)

      ttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCg                                                                                >  1:3140199/1‑90 (MQ=255)

      ttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCg                                                                                <  1:2596711/90‑1 (MQ=255)

         cGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCaa                                                                             >  1:90391/1‑90 (MQ=255)

         cGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCaa                                                                             <  1:2138008/90‑1 (MQ=255)

           cAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAata                                                                           >  1:2711611/1‑90 (MQ=255)

           cAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAata                                                                           >  1:1588808/1‑90 (MQ=255)

             gTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAAtata                                                                         <  1:3170788/90‑1 (MQ=255)

              tAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATAc                                                                        >  1:543410/1‑90 (MQ=255)

               aaaCTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACa                                                                       >  2:2885560/1‑90 (MQ=255)

                 aCTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACAtt                                                                     >  1:1508961/1‑90 (MQ=255)

                 aCTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACAtt                                                                     <  1:1780022/90‑1 (MQ=255)

                  cTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTg                                                                    >  1:1332106/1‑90 (MQ=255)

                  cTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTg                                                                    >  2:1918361/1‑90 (MQ=255)

                  cTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTg                                                                    <  1:2032654/90‑1 (MQ=255)

                   tAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTgg                                                                   >  2:1556473/1‑90 (MQ=255)

                      aaGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCga                                                                >  1:961403/1‑90 (MQ=255)

                      aaGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCga                                                                >  2:1088521/1‑90 (MQ=255)

                      aaGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCga                                                                <  2:1171935/90‑1 (MQ=255)

                      aaGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCga                                                                <  1:1231334/90‑1 (MQ=255)

                       aGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCgag                                                               >  2:3262390/1‑90 (MQ=255)

                        gTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCgaga                                                              >  2:2594913/1‑90 (MQ=255)

                        gTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCgaga                                                              <  2:1580506/90‑1 (MQ=255)

                        gTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCgaga                                                              >  2:2235853/1‑90 (MQ=255)

                         ttGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGagag                                                             >  2:3154141/1‑90 (MQ=255)

                          tGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGc                                                            >  1:1607718/1‑90 (MQ=255)

                           gCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCa                                                           <  1:2279643/90‑1 (MQ=255)

                            cccGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCAt                                                          >  1:2229042/1‑90 (MQ=255)

                             ccGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCAtt                                                         <  2:3223962/90‑1 (MQ=255)

                             ccGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCAtt                                                         >  2:302527/1‑90 (MQ=255)

                             ccGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCAtt                                                         >  2:1085373/1‑90 (MQ=255)

                                aaaaTAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAg                                                      >  2:1026322/1‑90 (MQ=255)

                                 aaaTAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTTAgg                                                     <  2:2138614/90‑1 (MQ=255)

                                 aaaTAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAgg                                                     <  1:3004896/90‑1 (MQ=255)

                                  aataatCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGc                                                    >  1:499279/1‑90 (MQ=255)

                                  aataatCGAAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGc                                                    >  2:2749311/1‑90 (MQ=255)

                                   ataatCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCt                                                   >  2:3295257/1‑90 (MQ=255)

                                   ataatCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCt                                                   >  1:143095/1‑90 (MQ=255)

                                    taatCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGTTa                                                  <  2:1793976/90‑1 (MQ=255)

                                     aatCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGGTaa                                                 >  1:277020/1‑90 (MQ=255)

                                     aatCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTaa                                                 >  1:750484/1‑90 (MQ=255)

                                      atCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAACATGAGCTGGAGTCAGTTCCGCAATAGACATTGGCGAGAGCATTGAGGCTaaa                                                >  1:1575219/1‑90 (MQ=255)

                                      atCGCAAACTGGGTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTaaa                                                <  2:1222296/90‑1 (MQ=255)

                                        cGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAAcc                                              <  2:2232408/90‑1 (MQ=255)

                                          cAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCaa                                            >  2:3389995/1‑90 (MQ=255)

                                          cAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCaa                                            >  1:2015932/1‑90 (MQ=255)

                                          cAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCaa                                            >  1:2957877/1‑90 (MQ=255)

                                           aaaCTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAAc                                           >  1:2926346/1‑90 (MQ=255)

                                           aaaCTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAAc                                           >  2:281200/1‑90 (MQ=255)

                                           aaaCTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAAc                                           >  2:690012/1‑90 (MQ=255)

                                            aaCTGGTTAAAGGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACg                                          >  1:243920/1‑90 (MQ=255)

                                            aaCTGGTTAAAAGAGCCCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACg                                          <  1:1296717/90‑1 (MQ=255)

                                              cTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGcc                                        >  1:368858/1‑90 (MQ=255)

                                              cTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGcc                                        >  2:2846885/1‑90 (MQ=255)

                                               tGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCa                                       <  1:3231083/90‑1 (MQ=255)

                                               tGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCa                                       <  1:3183820/90‑1 (MQ=255)

                                                ggTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCaa                                      <  2:658488/90‑1 (MQ=255)

                                                 gTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAAt                                     <  2:889066/90‑1 (MQ=255)

                                                 gTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAAt                                     >  2:482623/1‑90 (MQ=255)

                                                  ttAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATa                                    >  2:624791/1‑90 (MQ=255)

                                                   tAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATAc                                   >  2:2710870/1‑90 (MQ=255)

                                                    aaaaGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATAcc                                  >  1:2144802/1‑90 (MQ=255)

                                                    aaaaGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATAcc                                  <  2:605697/90‑1 (MQ=255)

                                                    aaaaGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATAcc                                  <  2:2010708/90‑1 (MQ=255)

                                                    aaaaGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGACTAAACCAACGCCAATAcc                                  >  1:1927332/1‑90 (MQ=255)

                                                     aaaGAGACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCg                                 <  2:90215/90‑1 (MQ=255)

                                                        gagaCCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCa                              <  1:3288767/90‑1 (MQ=255)

                                                          gaCCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCaat                            >  1:1072079/1‑90 (MQ=255)

                                                            ccAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCaataa                          <  1:1255267/90‑1 (MQ=255)

                                                             cAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCaataat                         <  2:1381950/90‑1 (MQ=255)

                                                             cAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCaataat                         <  2:72519/90‑1 (MQ=255)

                                                             cAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCAAGAGCATTGAGGCTAAACCAGCGCCAATACCGCCaataat                         <  2:1778943/90‑1 (MQ=255)

                                                              aGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATg                        <  1:2962249/90‑1 (MQ=255)

                                                               gTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGc                       >  1:1164428/1‑90 (MQ=255)

                                                               gTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGc                       >  2:858249/1‑90 (MQ=255)

                                                                tttCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCg                      >  1:811237/1‑90 (MQ=255)

                                                                tttCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCg                      >  2:2936196/1‑90 (MQ=255)

                                                                tttCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCg                      >  2:3295311/1‑90 (MQ=255)

                                                                 ttCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATCCCCCCAATAATGCGa                     >  2:1151332/1‑90 (MQ=255)

                                                                  tCCCGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGat                    <  1:591354/90‑1 (MQ=255)

                                                                    ccGCGAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataa                  <  2:2043116/90‑1 (MQ=255)

                                                                     cgcgAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaa                 >  1:2314843/1‑90 (MQ=255)

                                                                      gcgAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaat                <  1:848745/90‑1 (MQ=255)

                                                                       cgAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaata               <  2:426488/90‑1 (MQ=255)

                                                                       cgAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaata               <  1:348811/90‑1 (MQ=255)

                                                                       cgAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaata               <  2:2967103/90‑1 (MQ=255)

                                                                        gAATATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaataa              <  2:2976213/90‑1 (MQ=255)

                                                                         aaTATGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAAc             <  2:2104536/90‑1 (MQ=255)

                                                                          atatGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACa            >  2:2088652/1‑90 (MQ=255)

                                                                          atatGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACa            <  1:261796/90‑1 (MQ=255)

                                                                          atatGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACa            <  2:1558093/90‑1 (MQ=255)

                                                                           tatGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACaa           >  1:2739017/1‑90 (MQ=255)

                                                                            atGAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACaaa          >  2:2068933/1‑90 (MQ=255)

                                                                              gAGCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAAtt        >  1:1025303/1‑90 (MQ=255)

                                                                              gAGCTGGAGTCAGTTCCGCAATACACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAAtt        >  2:819157/1‑90 (MQ=255)

                                                                                gCTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTcc      <  1:3000769/90‑1 (MQ=255)

                                                                                 cTGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCg     <  1:2554315/90‑1 (MQ=255)

                                                                                  tGGAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCgg    <  2:529573/90‑1 (MQ=255)

                                                                                   ggAGTCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCggg   <  1:3056343/90‑1 (MQ=255)

AATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGA  >  AC_000091/4245295‑4245468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`