BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	4,444,533	C→T	intergenic (+60/+19)	ytfK → / ← ytfL	hypothetical protein/predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	4,444,533	0	C	T	100.0%	578.7 / NA	146	intergenic (+60/+19)	ytfK/ytfL	hypothetical protein/predicted inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (60/86); total (60/86)

Only 100 of 147 total aligned reads displayed.

ATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGT  >  AC_000091/4444447‑4444619

aTGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGt                                                                                     >  1:443008/1‑90 (MQ=255)

aTGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGt                                                                                     >  1:2526066/1‑90 (MQ=255)

  gCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTg                                                                                   <  1:40796/90‑1 (MQ=255)

  gCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTg                                                                                   <  1:3442024/90‑1 (MQ=255)

    gTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGAt                                                                                 <  1:517788/90‑1 (MQ=255)

        gCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGttt                                                                             <  1:1051988/90‑1 (MQ=255)

         cAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTc                                                                            >  1:3012297/1‑90 (MQ=255)

         cAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTc                                                                            <  1:1111698/90‑1 (MQ=255)

         cAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTc                                                                            <  1:2870161/90‑1 (MQ=255)

          aaaCAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCt                                                                           <  1:2969137/90‑1 (MQ=255)

          aaaCAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCt                                                                           <  1:3036115/90‑1 (MQ=255)

          aaaCAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCt                                                                           >  2:2923730/1‑90 (MQ=255)

          aaaCAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCt                                                                           <  2:1281101/90‑1 (MQ=255)

          aaaCAGAGATGGCTTAACCAAAGCGCTATGCAGTAAAAAGTGCTATGCAATAATAAGACGGCTACTGATTCAGGAGTCGTTGATGTTTCt                                                                           <  2:502291/90‑1 (MQ=255)

           aaCAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTg                                                                          <  2:3042963/90‑1 (MQ=255)

           aaCAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTg                                                                          <  1:475466/90‑1 (MQ=255)

            aCAGAGATGGGTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTgg                                                                         <  1:693931/90‑1 (MQ=255)

              agagATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTgggg                                                                       >  1:1541139/1‑90 (MQ=255)

              agagATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTgggg                                                                       >  2:1786099/1‑90 (MQ=255)

                  aTGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTa                                                                   >  1:3344454/1‑90 (MQ=255)

                  aTGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGCAGTCGTTGATGTTTCTGGGGGTTa                                                                   <  2:3235422/90‑1 (MQ=255)

                   tGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTAc                                                                  <  2:1947543/90‑1 (MQ=255)

                    ggCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTAcg                                                                 >  2:614840/1‑90 (MQ=255)

                      cTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTAcgcg                                                               >  1:2422756/1‑90 (MQ=255)

                      cTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTAcgcg                                                               >  2:2703247/1‑90 (MQ=255)

                        tAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGAc                                                             <  2:564521/90‑1 (MQ=255)

                        tAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGAc                                                             <  2:1128334/90‑1 (MQ=255)

                          aCCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGc                                                           <  2:83003/90‑1 (MQ=255)

                             aaagtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCttt                                                        >  1:989318/1‑90 (MQ=255)

                              aagtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTttc                                                       >  1:3120245/1‑90 (MQ=255)

                              aagagctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTttc                                                       >  2:3031293/1‑90 (MQ=255)

                               agtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTttct                                                      <  2:1899317/90‑1 (MQ=255)

                               agtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTttct                                                      <  2:1644982/90‑1 (MQ=255)

                                 tgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTttcttc                                                    <  1:2552221/90‑1 (MQ=255)

                                  gctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTtcttct                                                   <  1:2333178/90‑1 (MQ=255)

                                  gctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTtcttct                                                   <  2:2334228/90‑1 (MQ=255)

                                   ctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTcttctt                                                  <  1:11890/90‑1 (MQ=255)

                                     atgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtcttta                                                <  1:1029722/90‑1 (MQ=255)

                                      tgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttat                                               >  2:3050486/1‑90 (MQ=255)

                                      tgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttat                                               >  2:1915285/1‑90 (MQ=255)

                                      tgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttat                                               <  2:906641/90‑1 (MQ=255)

                                       gcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttatc                                              >  1:1934034/1‑90 (MQ=255)

                                       gcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttatc                                              <  2:1985438/90‑1 (MQ=255)

                                        cagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttatct                                             <  1:2842600/90‑1 (MQ=255)

                                         agtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttatctt                                            >  2:1743939/1‑90 (MQ=255)

                                           taaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttatcttta                                          <  1:1886403/90‑1 (MQ=255)

                                            aaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAg                                         >  2:2090903/1‑90 (MQ=255)

                                            aaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAg                                         <  2:897748/90‑1 (MQ=255)

                                            aaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAg                                         <  1:1035962/90‑1 (MQ=255)

                                             aaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGc                                        >  2:509319/1‑90 (MQ=255)

                                              aaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCg                                       <  1:2164226/90‑1 (MQ=255)

                                               aagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGt                                      >  1:496195/1‑90 (MQ=255)

                                                agtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTc                                     >  2:1745206/1‑90 (MQ=255)

                                                 gtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCa                                    <  1:993905/90‑1 (MQ=255)

                                                  tgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAg                                   >  2:2498214/1‑90 (MQ=255)

                                                  tgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAg                                   <  1:3303080/90‑1 (MQ=255)

                                                   gctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAgg                                  <  2:2881137/90‑1 (MQ=255)

                                                   gctatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAgg                                  <  2:2889709/90‑1 (MQ=255)

                                                     tatgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCa                                <  1:89852/90‑1 (MQ=255)

                                                      atgcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAg                               >  2:2057241/1‑90 (MQ=255)

                                                       agcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGt                              <  2:2569361/89‑1 (MQ=255)

                                                        gcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGtt                             <  2:291211/90‑1 (MQ=255)

                                                        gcagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGtt                             >  2:2686259/1‑90 (MQ=255)

                                                         cagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGttt                            >  1:1674104/1‑90 (MQ=255)

                                                         cagtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGttt                            <  1:2459874/90‑1 (MQ=255)

                                                          agtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGtttt                           <  2:805856/90‑1 (MQ=255)

                                                          agtaaTAAGACGGCTCCTGAGTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGtttt                           >  2:3323651/1‑90 (MQ=255)

                                                           gtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTg                          <  1:2677286/90‑1 (MQ=255)

                                                           gtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTg                          <  1:1405687/90‑1 (MQ=255)

                                                           gtaaTAAGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTg                          <  1:393558/90‑1 (MQ=255)

                                                             aataaGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGc                        >  2:2277828/1‑90 (MQ=255)

                                                             aataaGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGc                        <  1:3227557/90‑1 (MQ=255)

                                                              ataaGACGGCTCCTGATTCAGGAGTCGTTGATGTTTTTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCGGTTTTGGCg                       >  2:1513324/1‑90 (MQ=255)

                                                              ataaGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCg                       >  1:1251076/1‑90 (MQ=255)

                                                               taaGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGa                      >  1:158799/1‑90 (MQ=255)

                                                               taaGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGa                      <  1:165853/90‑1 (MQ=255)

                                                               taaGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGa                      <  1:1897936/90‑1 (MQ=255)

                                                               taaGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGGTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGa                      >  1:1371790/1‑90 (MQ=255)

                                                                aaGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGaa                     >  1:762655/1‑90 (MQ=255)

                                                                aaGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGaa                     >  2:1035124/1‑90 (MQ=255)

                                                                 aGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGaaa                    <  2:3373054/90‑1 (MQ=255)

                                                                 aGACGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGaaa                    <  2:2374679/90‑1 (MQ=255)

                                                                   aCGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAgg                  <  1:1286821/90‑1 (MQ=255)

                                                                   aCGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAgg                  <  1:1477249/90‑1 (MQ=255)

                                                                    cGGCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAggg                 <  2:1335098/90‑1 (MQ=255)

                                                                     ggCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGc                <  2:720138/90‑1 (MQ=255)

                                                                     ggCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGc                >  1:2623782/1‑90 (MQ=255)

                                                                      gCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCg               >  1:2160302/1‑90 (MQ=255)

                                                                      gCTCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCg               <  1:3198408/90‑1 (MQ=255)

                                                                        tCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGt             >  2:1991489/1‑90 (MQ=255)

                                                                        tCCTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGt             <  2:3258954/90‑1 (MQ=255)

                                                                         ccTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTg            >  1:3344792/1‑90 (MQ=255)

                                                                         ccTGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTg            <  2:1776158/90‑1 (MQ=255)

                                                                           tGATTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGc          <  1:3367348/90‑1 (MQ=255)

                                                                             atccAGGAGTCGCTGATGTCTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCt        <  2:2058906/87‑1 (MQ=255)

                                                                             aTTCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCt        >  2:719112/1‑90 (MQ=255)

                                                                              ttCAGGAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCtt       >  1:2239153/1‑90 (MQ=255)

                                                                                  ggAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTg   >  1:2150163/1‑90 (MQ=255)

                                                                                   gAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGt  >  1:1096037/1‑90 (MQ=255)

                                                                                   gAGTCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGt  >  1:1842505/1‑90 (MQ=255)

ATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGT  >  AC_000091/4444447‑4444619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`