Predicted mutation
evidence seq id position mutation annotation gene description
RA AC_000091 4,558,099 T→C R173R (CGT→CGC)  uxuB → D‑mannonate oxidoreductase, NAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000914,558,0990TC100.0% 504.3 / NA 129R173R (CGT→CGC) uxuBD‑mannonate oxidoreductase, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (56/73);  total (56/73)

Only 100 of 136 total aligned reads displayed.

TAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCT  >  AC_000091/4558012‑4558185
                                                                                       |                                                                                      
tAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGa                                                                                      <  1:1327028/90‑1 (MQ=255)
  aCCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGaaa                                                                                    >  2:2337335/1‑90 (MQ=255)
  aCCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGaaa                                                                                    <  1:1741427/90‑1 (MQ=255)
  aCCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGaaa                                                                                    >  1:79193/1‑90 (MQ=255)
    ccGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGaaaaa                                                                                  <  2:2898822/90‑1 (MQ=255)
    ccGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGaaaaa                                                                                  <  2:2548408/90‑1 (MQ=255)
     cGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAg                                                                                 >  2:2226239/1‑90 (MQ=255)
     cGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAg                                                                                 <  2:1340363/90‑1 (MQ=255)
      gCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAgg                                                                                >  2:2906372/1‑90 (MQ=255)
      gCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAgg                                                                                <  2:3300199/90‑1 (MQ=255)
      gCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAgg                                                                                <  1:215139/90‑1 (MQ=255)
      gCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAgg                                                                                <  2:3207634/90‑1 (MQ=255)
       cTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAggg                                                                               <  2:2772348/90‑1 (MQ=255)
        tGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGt                                                                              <  2:2962496/90‑1 (MQ=255)
          aTCAAGCACGATCTGGAAACCCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTg                                                                            <  2:2780513/90‑1 (MQ=255)
           tCAAGCACGATCTGGAAACCCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGa                                                                           <  1:3170671/90‑1 (MQ=255)
           tCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGa                                                                           >  1:2475506/1‑90 (MQ=255)
           tCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGa                                                                           >  1:2230087/1‑90 (MQ=255)
           tCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGa                                                                           <  1:1244099/90‑1 (MQ=255)
            cAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGaa                                                                          >  2:3138113/1‑90 (MQ=255)
             aaGCACGATCTGGAAACCCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGaaa                                                                         <  2:272384/90‑1 (MQ=255)
             aaGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGaaa                                                                         <  2:1802759/90‑1 (MQ=255)
             aaGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGaaa                                                                         <  2:541696/90‑1 (MQ=255)
             aaGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGaaa                                                                         <  1:1690363/90‑1 (MQ=255)
             aaGCACGATCAGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGCTGCGTCTGCGTCGCGAAAAAGGGTTGaaa                                                                         <  2:3290966/90‑1 (MQ=255)
              aGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAg                                                                        <  1:3335116/90‑1 (MQ=255)
              aGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAg                                                                        >  2:2354781/1‑90 (MQ=255)
               gCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGc                                                                       >  2:3105021/1‑90 (MQ=255)
                cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCg                                                                      <  2:2983864/90‑1 (MQ=255)
                cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCg                                                                      >  2:2455395/1‑90 (MQ=255)
                cACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCg                                                                      <  1:1846009/90‑1 (MQ=255)
                 aCGATCTGGAAACCCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGt                                                                     <  2:1504245/90‑1 (MQ=255)
                  cGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGtt                                                                    <  2:3299053/90‑1 (MQ=255)
                   gATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGttt                                                                   <  2:1879957/90‑1 (MQ=255)
                   gATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGttt                                                                   >  2:3054959/1‑90 (MQ=255)
                   gATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGttt                                                                   >  2:1317193/1‑90 (MQ=255)
                   gATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGttt                                                                   <  2:3085808/90‑1 (MQ=255)
                   gATCTGGAAAACCCGACTGCGCCGAAGCCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGttt                                                                   <  2:2784467/90‑1 (MQ=255)
                    aTCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTa                                                                  <  1:2453752/90‑1 (MQ=255)
                    aTCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTa                                                                  <  2:238631/90‑1 (MQ=255)
                    aTCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTa                                                                  >  1:807464/1‑90 (MQ=255)
                    aTCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTa                                                                  >  1:2027342/1‑90 (MQ=255)
                      cTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACg                                                                <  2:1952677/90‑1 (MQ=255)
                       tGGAAAACCCGACTGCGCCGGAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACgg                                                               <  1:822747/90‑1 (MQ=255)
                       tGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACgg                                                               >  2:3470620/1‑90 (MQ=255)
                       tGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACgg                                                               >  2:1714138/1‑90 (MQ=255)
                        ggAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGt                                                              <  2:3157170/90‑1 (MQ=255)
                         gAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTg                                                             >  1:1177013/1‑90 (MQ=255)
                         gAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTg                                                             >  1:2532508/1‑90 (MQ=255)
                          aaaaCCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGa                                                            <  2:1719249/90‑1 (MQ=255)
                          aaaaCCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGa                                                            >  1:2921411/1‑90 (MQ=255)
                           aaaCCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGAt                                                           <  2:2023401/90‑1 (MQ=255)
                            aaCCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATg                                                          >  2:969117/1‑90 (MQ=255)
                            aaCCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGGTGAAAGCGTTTACGGTGATg                                                          >  2:2196373/1‑90 (MQ=255)
                              cccGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTc                                                        <  2:924173/90‑1 (MQ=255)
                              cccGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTc                                                        <  1:685252/90‑1 (MQ=255)
                               ccGACTGCGCCGAAGTCCGCGATTCGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTcc                                                       <  1:2839056/90‑1 (MQ=255)
                                cGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCt                                                      >  2:592235/1‑90 (MQ=255)
                                cGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCt                                                      <  2:438192/90‑1 (MQ=255)
                                 gACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg                                                     >  1:2234180/1‑90 (MQ=255)
                                 gACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg                                                     >  2:2299171/1‑90 (MQ=255)
                                 gACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg                                                     <  2:1451811/90‑1 (MQ=255)
                                 gACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg                                                     <  2:742849/90‑1 (MQ=255)
                                    tGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGa                                                  <  1:1338818/90‑1 (MQ=255)
                                     gcgcCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGAt                                                 <  2:1486307/90‑1 (MQ=255)
                                      cgcCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa                                                >  1:3299072/1‑90 (MQ=255)
                                        ccGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAAc                                              >  2:954069/1‑90 (MQ=255)
                                         cGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAAcg                                             >  1:897857/1‑90 (MQ=255)
                                          gAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAAcgt                                            <  2:1333403/90‑1 (MQ=255)
                                          gAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAAcgt                                            >  2:1165349/1‑90 (MQ=255)
                                           aaGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAAcgtg                                           <  2:2132523/90‑1 (MQ=255)
                                           aaGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAAcgtg                                           <  1:889194/90‑1 (MQ=255)
                                            aGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAAcgtgc                                          <  1:1061944/90‑1 (MQ=255)
                                                cgcgATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGa                                      >  1:3177431/1‑90 (MQ=255)
                                                     ttGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACg                                 <  2:956250/90‑1 (MQ=255)
                                                      tGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACgg                                <  2:1400227/90‑1 (MQ=255)
                                                      tGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTCAAAACgg                                <  2:700055/90‑1 (MQ=255)
                                                         ttACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCa                             <  1:3467403/90‑1 (MQ=255)
                                                         ttACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCa                             >  2:2712353/1‑90 (MQ=255)
                                                          tACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCAt                            <  2:975815/90‑1 (MQ=255)
                                                          tACATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCAt                            <  1:951889/90‑1 (MQ=255)
                                                           aCATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCAtg                           <  1:1508130/90‑1 (MQ=255)
                                                            cATCGTCGAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCAtgt                          >  1:302294/1‑90 (MQ=255)
                                                                gtcgAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCg                      >  2:236685/1‑90 (MQ=255)
                                                                   gAAGTGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGCCGAAg                   <  2:2852685/90‑1 (MQ=255)
                                                                   gAAGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAg                   >  1:513996/1‑90 (MQ=255)
                                                                    aaGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAgg                  >  2:1321231/1‑90 (MQ=255)
                                                                    aaGCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAgg                  >  1:132594/1‑90 (MQ=255)
                                                                      gCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTc                <  2:2373801/90‑1 (MQ=255)
                                                                      gCGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTc                >  1:88/1‑90 (MQ=255)
                                                                       cGTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTcg               <  2:3051675/90‑1 (MQ=255)
                                                                        gTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTcgc              >  2:1918586/1‑90 (MQ=255)
                                                                        gTTGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTcgc              >  2:3474069/1‑90 (MQ=255)
                                                                         ttGCGTCTGCGTCGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTcgcg             >  2:3032110/1‑90 (MQ=255)
                                                                             gtctgcgtcGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTAc         >  2:2737392/1‑90 (MQ=255)
                                                                               ctgcgtcGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTg       >  2:85552/1‑90 (MQ=255)
                                                                                tgcgtcGCGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTgg      <  2:2193645/90‑1 (MQ=255)
                                                                                  cgtcgcGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTgggg    >  2:3248337/1‑90 (MQ=255)
                                                                                  cgtcgcGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTgggg    <  1:3383526/90‑1 (MQ=255)
                                                                                  cgtcgcGAAAAAGGGTTGAAAGCGTTTACGGTCATGTCCTGCGATAACGTGCGTG‑AAACGGTCATGTGGCGAAGGTCGCGGTACTGGggc   <  2:1157221/90‑1 (MQ=255)
                                                                                       |                                                                                      
TAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCT  >  AC_000091/4558012‑4558185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑