BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	366,525	A→G	S70S (AGT→AGC)	lacI ←	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	366,525	0	A	G	100.0%	442.2 / NA	113	S70S (AGT→AGC)	lacI	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (52/61); total (52/61)

Only 100 of 115 total aligned reads displayed.

TACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCAT  >  AC_000091/366438‑366611

tACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCt                                                                                      <  2:2205793/90‑1 (MQ=255)

tACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCt                                                                                      <  2:378564/90‑1 (MQ=255)

tACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCt                                                                                      >  1:35353/1‑90 (MQ=255)

tACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCt                                                                                      <  2:1343092/90‑1 (MQ=255)

  ccATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTgg                                                                                    >  2:666360/1‑90 (MQ=255)

  ccATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTgg                                                                                    <  1:3469053/90‑1 (MQ=255)

  ccATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTgg                                                                                    >  2:1481389/1‑90 (MQ=255)

   cATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGa                                                                                   >  1:2765048/1‑90 (MQ=255)

       gACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTg                                                                               <  1:1430051/90‑1 (MQ=255)

            caccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAAc                                                                          <  1:2267529/90‑1 (MQ=255)

            caccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAAc                                                                          <  1:1200552/90‑1 (MQ=255)

            caccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAAc                                                                          <  1:1899413/90‑1 (MQ=255)

             accacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACg                                                                         <  1:1655781/90‑1 (MQ=255)

              ccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGc                                                                        <  2:3440505/90‑1 (MQ=255)

               cacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGcc                                                                       >  2:2529327/1‑90 (MQ=255)

               cacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGcc                                                                       <  1:1639972/90‑1 (MQ=255)

                   cTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATc                                                                   <  2:1876900/90‑1 (MQ=255)

                   cTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATc                                                                   <  1:372317/90‑1 (MQ=255)

                    tGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCa                                                                  >  2:3128153/1‑90 (MQ=255)

                     ggCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAg                                                                 <  1:1012280/90‑1 (MQ=255)

                      gCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGc                                                                <  1:1725737/90‑1 (MQ=255)

                        aCCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTACGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCaa                                                              <  2:1948904/90‑1 (MQ=255)

                          ccAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACg                                                            >  1:27129/1‑90 (MQ=255)

                          ccACTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACg                                                            <  2:1027907/90‑1 (MQ=255)

                           cAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGa                                                           >  1:430677/1‑90 (MQ=255)

                             gTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACt                                                         <  1:1209912/90‑1 (MQ=255)

                               tGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGt                                                       >  1:2490765/1‑90 (MQ=255)

                                gATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGtt                                                      <  2:580261/90‑1 (MQ=255)

                                gATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGtt                                                      <  2:1201530/90‑1 (MQ=255)

                                gATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGtt                                                      <  1:116068/90‑1 (MQ=255)

                                gATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGtt                                                      >  1:337236/1‑90 (MQ=255)

                                 aTCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGttt                                                     <  1:172676/90‑1 (MQ=255)

                                  tCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTg                                                    <  2:2390252/90‑1 (MQ=255)

                                   cGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGc                                                   >  2:2053955/1‑90 (MQ=255)

                                    ggCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGcc                                                  >  1:2163964/1‑90 (MQ=255)

                                    ggCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGcc                                                  >  1:394296/1‑90 (MQ=255)

                                     gcgcGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGccc                                                 >  2:2938533/1‑90 (MQ=255)

                                       gcgAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGc                                               >  1:3394890/1‑90 (MQ=255)

                                       gcgAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGc                                               <  1:3322715/90‑1 (MQ=255)

                                         gagaTTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCa                                             >  2:3073450/1‑90 (MQ=255)

                                         gagaTTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCa                                             <  1:653154/90‑1 (MQ=255)

                                          agaTTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAg                                            <  2:806649/90‑1 (MQ=255)

                                          agaTTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAg                                            >  1:1687622/1‑90 (MQ=255)

                                          agaTTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAg                                            <  1:557840/90‑1 (MQ=255)

                                           gaTTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAgt                                           >  1:1642392/1‑90 (MQ=255)

                                            aTTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAgtt                                          <  2:2056547/90‑1 (MQ=255)

                                            aTTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAgtt                                          >  1:1274835/1‑90 (MQ=255)

                                            aTTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAgtt                                          <  1:3379980/90‑1 (MQ=255)

                                               tAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAgttgtt                                       >  2:3171348/1‑90 (MQ=255)

                                               tAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAgttgtt                                       >  2:803286/1‑90 (MQ=255)

                                                aaTCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCCACGCCAATCAGCAACGACTGTTTGCCCGCCAGttgttg                                      >  2:1666161/1‑90 (MQ=255)

                                                aaTCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGttgttg                                      >  1:553414/1‑90 (MQ=255)

                                                aaTCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGttgttg                                      >  2:786913/1‑90 (MQ=255)

                                                 aTCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTtgttgt                                     <  1:2232643/90‑1 (MQ=255)

                                                  tCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTtgtg                                    >  1:3131950/1‑90 (MQ=255)

                                                   cgccgcGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGc                                   <  2:550724/90‑1 (MQ=255)

                                                   cgccgcGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGc                                   >  2:2449481/1‑90 (MQ=255)

                                                   cgccgcGACAATTTGCGACGGCGCGTGCAGCGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGc                                   <  1:974467/90‑1 (MQ=255)

                                                    gccgcGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGcc                                  <  2:422956/90‑1 (MQ=255)

                                                     ccgcGACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCa                                 >  2:1264692/1‑90 (MQ=255)

                                                       gcgACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAcg                               >  1:3332053/1‑90 (MQ=255)

                                                        cgACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAcgc                              >  2:3241768/1‑90 (MQ=255)

                                                        cgACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAcgc                              >  1:2460525/1‑90 (MQ=255)

                                                         gACAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGCCAACGCCAATCAGCAACGGCTGTTTGCCCGCCAGTTGTTGTGCCAcgcg                             <  2:2794657/90‑1 (MQ=255)

                                                          aCAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCgg                            <  2:1309826/90‑1 (MQ=255)

                                                           cAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGt                           >  1:2094408/1‑90 (MQ=255)

                                                           cAATTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGt                           <  2:2183799/90‑1 (MQ=255)

                                                            aaTTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGtt                          >  2:2224019/1‑90 (MQ=255)

                                                            aaTTTGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTGGTTGTGCCACGCGGtt                          >  2:124097/1‑90 (MQ=255)

                                                              tttGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTgg                        >  2:916416/1‑90 (MQ=255)

                                                               ttGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTggg                       >  2:4665/1‑90 (MQ=255)

                                                                tGCGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGa                      >  1:328029/1‑90 (MQ=255)

                                                                  cGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAAt                    <  1:1958831/90‑1 (MQ=255)

                                                                  cGACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAAt                    >  2:189184/1‑90 (MQ=255)

                                                                  cGACGGCGCGTGCAGGGCCAGGCTGGAGGGGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAAt                    >  2:751140/1‑90 (MQ=255)

                                                                   gACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATg                   <  1:113186/90‑1 (MQ=255)

                                                                   gACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATg                   <  2:1814138/90‑1 (MQ=255)

                                                                   gACGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATg                   <  1:3485271/90‑1 (MQ=255)

                                                                    aCGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGt                  <  1:602881/90‑1 (MQ=255)

                                                                     cGGCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTa                 >  2:1340813/1‑90 (MQ=255)

                                                                      ggCGCGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTaa                <  1:72797/90‑1 (MQ=255)

                                                                       gcgcGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATGAGCAACGCCTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAAt               <  2:1631333/90‑1 (MQ=255)

                                                                       gcgcGTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAAt               <  2:817267/90‑1 (MQ=255)

                                                                        cgcgTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAAtt              <  1:920266/90‑1 (MQ=255)

                                                                         gcgTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTc             <  2:1150932/90‑1 (MQ=255)

                                                                          cgTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCa            >  1:99664/1‑90 (MQ=255)

                                                                          cgTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCa            <  2:902304/90‑1 (MQ=255)

                                                                           gTGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAg           <  2:156409/90‑1 (MQ=255)

                                                                           atGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAg           <  2:3435983/89‑1 (MQ=255)

                                                                            tGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGc          >  1:1969664/1‑90 (MQ=255)

                                                                            tGCAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTATTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGc          >  2:2193635/1‑90 (MQ=255)

                                                                              cAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTc        <  1:924834/90‑1 (MQ=255)

                                                                              cAGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTc        >  1:923197/1‑90 (MQ=255)

                                                                               aGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTcc       <  1:487927/90‑1 (MQ=255)

                                                                               aGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTcc       <  2:560691/90‑1 (MQ=255)

                                                                               aGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTcc       <  1:2581419/90‑1 (MQ=255)

                                                                               aGGGCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTcc       <  1:1858163/90‑1 (MQ=255)

                                                                                gggCCAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCg      >  1:156962/1‑90 (MQ=255)

                                                                                    cAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCAt  >  2:3387681/1‑90 (MQ=255)

                                                                                    cAGGCTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCAt  <  1:748541/90‑1 (MQ=255)

TACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCAT  >  AC_000091/366438‑366611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`