BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	457,230	A→G	D194G (GAC→GGC)	clpX →	ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	457,230	0	A	G	100.0%	532.9 / NA	137	D194G (GAC→GGC)	clpX	ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (70/67); total (70/67)

Only 100 of 138 total aligned reads displayed.

TGTTGCAGAAATGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTT  >  AC_000091/457143‑457315

tGTTGCAGAAATGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCa                                                                                     >  1:1946557/1‑90 (MQ=255)

 gTTGCAGAAATGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCaa                                                                                    >  1:72997/1‑90 (MQ=255)

   tGCAGAAATGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAAcc                                                                                  <  1:2869588/90‑1 (MQ=255)

   tGCAGAAATGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAAcc                                                                                  <  2:1747718/90‑1 (MQ=255)

     cAGAAATGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTCAGTCAGGCAACCCg                                                                                <  2:3373335/90‑1 (MQ=255)

     cAGAAATGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCg                                                                                <  1:1883685/90‑1 (MQ=255)

        aaaTGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTcc                                                                             <  2:1429022/90‑1 (MQ=255)

         aaTGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCa                                                                            >  2:2585694/1‑90 (MQ=255)

         aaTGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCa                                                                            >  2:2290670/1‑90 (MQ=255)

         aaTGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCa                                                                            >  2:647980/1‑90 (MQ=255)

          aTGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCAt                                                                           >  1:312108/1‑90 (MQ=255)

           tGCGACTACGATGTCCAGAAAGCAGAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCAtt                                                                          >  2:2264896/1‑90 (MQ=255)

           tGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCAtt                                                                          >  2:491745/1‑90 (MQ=255)

             cGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTAc                                                                        <  2:1854513/90‑1 (MQ=255)

             cGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTAc                                                                        <  1:449507/90‑1 (MQ=255)

              gACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTAcc                                                                       <  1:1460158/90‑1 (MQ=255)

              gACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTAcc                                                                       >  2:1965517/1‑90 (MQ=255)

              gACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTAAGGCAACCCGTCCATTAcc                                                                       >  2:1629702/1‑90 (MQ=255)

               aCTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTAccc                                                                      >  2:2325625/1‑90 (MQ=255)

               aCTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTAccc                                                                      >  1:1147070/1‑90 (MQ=255)

                cTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCg                                                                     <  1:67105/90‑1 (MQ=255)

                cTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCg                                                                     <  1:169717/90‑1 (MQ=255)

                cTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCg                                                                     >  2:3080826/1‑90 (MQ=255)

                cTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCg                                                                     >  1:2656156/1‑90 (MQ=255)

                  aCGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCgag                                                                   <  1:1724823/90‑1 (MQ=255)

                   cGATGTCCAGAAAGCACAGCGTTGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCgaga                                                                  >  1:2089058/1‑90 (MQ=255)

                   cGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCgaga                                                                  >  1:1887600/1‑90 (MQ=255)

                    gATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGAc                                                                 >  1:2762270/1‑90 (MQ=255)

                     aTGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACACGTCCATTACCCGAGACg                                                                <  1:212122/90‑1 (MQ=255)

                       gTCCAGAAAGCACAGCTTGGTATTGTCTAAATCGATGATATCGATAAGATTTCTCGTAAGTCCGGCGACCCGTCCATTACCCGAGACGtt                                                              >  2:1473339/1‑90 (MQ=255)

                       gTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGtt                                                              >  1:155510/1‑90 (MQ=255)

                       gTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGtt                                                              <  1:381398/90‑1 (MQ=255)

                        tCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGttt                                                             >  1:2724421/1‑90 (MQ=255)

                         ccAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTc                                                            >  2:1971612/1‑90 (MQ=255)

                         ccAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTc                                                            <  1:3186001/90‑1 (MQ=255)

                          cAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTcc                                                           >  1:3162635/1‑90 (MQ=255)

                           aGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCg                                                          >  1:2572392/1‑90 (MQ=255)

                            gAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCgg                                                         <  1:118033/90‑1 (MQ=255)

                               aGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGa                                                      <  1:2387673/90‑1 (MQ=255)

                               aGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGa                                                      <  1:3131142/90‑1 (MQ=255)

                                  acaGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAgg                                                   <  2:1506950/90‑1 (MQ=255)

                                   caGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGc                                                  >  2:736579/1‑90 (MQ=255)

                                   caGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGc                                                  >  1:1114877/1‑90 (MQ=255)

                                   caGCGTGGTATTGGCTACATCTATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGc                                                  <  1:3395657/90‑1 (MQ=255)

                                    aGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCg                                                 >  1:3054301/1‑90 (MQ=255)

                                      cGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCTGAGACGTTTCCGGTGAAGGCGTa                                               >  2:3107729/1‑90 (MQ=255)

                                       ggggTATTGGCTACATCGATGAAATCGGCAAGATTTCTCGGAAGTCAGGCAACCCGTCCATTACCCGAGAGGTTTCCGGTGGAGGCGTAc                                              >  2:2486474/3‑90 (MQ=255)

                                         ggTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGCGACGTTTCCGGTGAAGGCGTAcag                                            <  2:1670512/90‑1 (MQ=255)

                                         ggTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTAcag                                            <  2:1344561/90‑1 (MQ=255)

                                           tATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTAcagca                                          <  2:1761520/90‑1 (MQ=255)

                                           tATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTAcagca                                          <  1:1932432/90‑1 (MQ=255)

                                             ttGTCTACATCTATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAgg                                        <  1:1263119/90‑1 (MQ=255)

                                              tGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGc                                       >  2:1178459/1‑90 (MQ=255)

                                                tCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGCTTCCGGTGAAGGCGTACAGCAGGCAc                                     >  2:1214597/1‑90 (MQ=255)

                                                 ctccATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCACCCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACt                                    <  2:2844050/87‑1 (MQ=255)

                                                 cTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACt                                    >  1:2792178/1‑90 (MQ=255)

                                                    cATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCACCCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGtt                                 <  2:1669030/90‑1 (MQ=255)

                                                    cATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGtt                                 <  2:2744135/90‑1 (MQ=255)

                                                    cATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGtt                                 <  1:667987/90‑1 (MQ=255)

                                                    cATCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGtt                                 >  1:3255625/1‑90 (MQ=255)

                                                     aTCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTg                                >  2:2740637/1‑90 (MQ=255)

                                                      tCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAGGGCGTACAGCAGGCACTGTTGa                               <  2:2897251/90‑1 (MQ=255)

                                                      tCGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGa                               <  2:39078/90‑1 (MQ=255)

                                                       cGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGaa                              >  2:1172395/1‑90 (MQ=255)

                                                       cGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGaa                              <  2:588199/90‑1 (MQ=255)

                                                       cGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGaa                              >  1:2017130/1‑90 (MQ=255)

                                                       cGATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGaa                              >  1:2356856/1‑90 (MQ=255)

                                                        gATGAAATCGACAAGATTTCTCGTAAGTCAGGCATCCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGaaa                             <  1:2513601/90‑1 (MQ=255)

                                                        gATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGaaa                             <  2:272978/90‑1 (MQ=255)

                                                        gATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGaaa                             >  2:709386/1‑90 (MQ=255)

                                                        gATGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGaaa                             >  2:1666613/1‑90 (MQ=255)

                                                         aTGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAAc                            >  1:2033047/1‑90 (MQ=255)

                                                          tGAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACt                           <  2:480874/90‑1 (MQ=255)

                                                           gAAATCGTCAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTg                          >  2:1340023/1‑90 (MQ=255)

                                                           gAAATCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTg                          >  1:1082130/1‑90 (MQ=255)

                                                            aaaTCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGa                         >  1:1678642/1‑90 (MQ=255)

                                                             aaTCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAt                        <  1:1861057/90‑1 (MQ=255)

                                                              aTCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATc                       >  2:104225/1‑90 (MQ=255)

                                                              aTCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATc                       <  2:1728119/90‑1 (MQ=255)

                                                               tCGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCg                      <  2:449877/90‑1 (MQ=255)

                                                                cGACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGa                     <  1:234407/90‑1 (MQ=255)

                                                                 gACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGaa                    >  1:3162638/1‑90 (MQ=255)

                                                                 gACAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGaa                    <  2:674393/90‑1 (MQ=255)

                                                                   cAAGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGCTGAAACTGATCGAAgg                  >  1:1564946/1‑90 (MQ=255)

                                                                    aaGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGt                 <  1:1113348/90‑1 (MQ=255)

                                                                    aaGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGt                 >  1:2943099/1‑90 (MQ=255)

                                                                     aGATTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTa                <  1:370857/90‑1 (MQ=255)

                                                                       aTTTCTCGTAAGTCAGGCATCCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACg              >  2:455008/1‑90 (MQ=255)

                                                                       aTTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACg              >  1:1757651/1‑90 (MQ=255)

                                                                       aTTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACg              <  2:3471159/90‑1 (MQ=255)

                                                                       aTTTCTCGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACg              >  1:3352080/1‑90 (MQ=255)

                                                                          tctcGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGAACGGTa           >  1:1358387/1‑90 (MQ=255)

                                                                           ctcGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAg          <  1:887671/90‑1 (MQ=255)

                                                                            tcGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGTCACTGTTGAAACTGATCGAAGGTACGGTAgc         >  1:3110847/1‑90 (MQ=255)

                                                                            tcGTAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAgc         <  1:522430/90‑1 (MQ=255)

                                                                               tAAGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAgctgc      >  2:2769160/1‑90 (MQ=255)

                                                                                aaGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAgctgct     <  2:326242/90‑1 (MQ=255)

                                                                                aaGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAgctgct     >  1:2523754/1‑90 (MQ=255)

                                                                                 aGTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGctgctg    <  1:1398341/90‑1 (MQ=255)

                                                                                  gTCAGGCAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGt   >  1:987812/1‑90 (MQ=255)

TGTTGCAGAAATGCGACTACGATGTCCAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTT  >  AC_000091/457143‑457315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`