BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	486,032	A→G	L91L (CTA→CTG)	kefA →	fused mechanosensitive channel proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	486,032	0	A	G	100.0%	524.2 / NA	133	L91L (CTA→CTG)	kefA	fused mechanosensitive channel proteins
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (78/55); total (78/55)

Only 100 of 134 total aligned reads displayed.

TCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAACGACGAAG  >  AC_000091/485945‑486117

 cAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCgg                                                                                    >  1:815445/1‑90 (MQ=255)

  aGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGc                                                                                   >  2:1541861/1‑90 (MQ=255)

    gACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCaa                                                                                 <  2:3284309/90‑1 (MQ=255)

    gACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCaa                                                                                 >  2:1504647/1‑90 (MQ=255)

      cAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCaaaa                                                                               >  1:2500548/1‑90 (MQ=255)

      cAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCaaaa                                                                               >  1:3119972/1‑90 (MQ=255)

        aaCTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAg                                                                             <  2:539761/90‑1 (MQ=255)

        aaCTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAg                                                                             >  1:1588457/1‑90 (MQ=255)

         aCTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGt                                                                            >  1:2532541/1‑90 (MQ=255)

          cTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTc                                                                           <  1:3027629/90‑1 (MQ=255)

           tGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCg                                                                          <  1:2877601/90‑1 (MQ=255)

            ggTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGc                                                                         >  1:44829/1‑90 (MQ=255)

             gTGCAGCAGGATCTGACAGATACATTAGCCCCCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCt                                                                        <  1:1487829/90‑1 (MQ=255)

             gTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCt                                                                        <  1:2665360/90‑1 (MQ=255)

             gTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCt                                                                        >  2:2920243/1‑90 (MQ=255)

              tGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTg                                                                       <  2:1256090/90‑1 (MQ=255)

               gcagcaGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGa                                                                      >  2:2412774/1‑90 (MQ=255)

                 agcagGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAg                                                                    >  1:609522/1‑90 (MQ=255)

                 agcagGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAg                                                                    <  1:489410/90‑1 (MQ=255)

                  gcagGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAgc                                                                   <  2:2532541/90‑1 (MQ=255)

                   cagGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAgcg                                                                  >  1:1007700/1‑90 (MQ=255)

                      gATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCg                                                               <  2:1215296/90‑1 (MQ=255)

                      gATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCg                                                               <  2:319379/90‑1 (MQ=255)

                      gATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCg                                                               >  1:1068036/1‑90 (MQ=255)

                       aTCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCgg                                                              <  1:1235496/90‑1 (MQ=255)

                        tCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGa                                                             <  1:2248041/90‑1 (MQ=255)

                         cTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGGAAAAAGTCGCTGAAGCGCCGGaa                                                            >  2:52392/1‑90 (MQ=255)

                         cTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCggga                                                            >  1:1141326/1‑88 (MQ=255)

                         cTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGaa                                                            <  2:425555/90‑1 (MQ=255)

                          tGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGaaa                                                           >  2:1287563/1‑90 (MQ=255)

                             cAGATACATTAGCCACCATCGATAAAATCGATAGCATAAAAGAAGAGACCGTTAAGCTGCGACAAAAAGACGCTGAAGCACCGGaaaaaa                                                        >  2:496992/1‑90 (MQ=255)

                              aGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAAt                                                       >  1:373660/1‑90 (MQ=255)

                              aGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAAt                                                       >  1:870091/1‑90 (MQ=255)

                               gATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATg                                                      <  1:3346057/90‑1 (MQ=255)

                                aTACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATgc                                                     <  2:1008636/90‑1 (MQ=255)

                                 tACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATgcg                                                    <  2:337882/90‑1 (MQ=255)

                                 tACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATgcg                                                    >  1:235665/1‑90 (MQ=255)

                                  aCATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATgcgc                                                   <  1:321839/90‑1 (MQ=255)

                                   cATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGcc                                                  <  2:3431567/90‑1 (MQ=255)

                                    aTTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCa                                                 <  1:2530118/90‑1 (MQ=255)

                                    aTTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCa                                                 <  1:656300/90‑1 (MQ=255)

                                     ttAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAg                                                >  2:772693/1‑90 (MQ=255)

                                     ttAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAg                                                >  2:217356/1‑90 (MQ=255)

                                        gCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCg                                             >  2:1602652/1‑90 (MQ=255)

                                         ccACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGa                                            <  2:1986029/90‑1 (MQ=255)

                                         ccACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGa                                            >  2:2406157/1‑90 (MQ=255)

                                         ccACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGa                                            >  1:550917/1‑90 (MQ=255)

                                               tCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCgcgg                                      >  1:1280317/1‑90 (MQ=255)

                                                cGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCgcggc                                     >  2:1035187/1‑90 (MQ=255)

                                                cGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCgcggc                                     <  1:3116853/90‑1 (MQ=255)

                                                 gATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCgcggcg                                    >  1:3443766/1‑90 (MQ=255)

                                                  aTAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGt                                   >  1:529447/1‑90 (MQ=255)

                                                  aTAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGt                                   >  1:1674183/1‑90 (MQ=255)

                                                  aTAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGt                                   >  1:2838045/1‑90 (MQ=255)

                                                   tAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGtt                                  <  2:1519501/90‑1 (MQ=255)

                                                    aaaaTCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTa                                 >  1:3353721/1‑90 (MQ=255)

                                                     aaaTCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGGGTTaa                                >  2:1255372/1‑90 (MQ=255)

                                                     aaaTCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTaa                                >  1:645489/1‑90 (MQ=255)

                                                     aaaTCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTaa                                >  2:1303504/1‑90 (MQ=255)

                                                      aaTCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAAc                               <  1:3448529/90‑1 (MQ=255)

                                                      aaTCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAAc                               >  1:261440/1‑90 (MQ=255)

                                                      aaTCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAAc                               >  1:1621600/1‑90 (MQ=255)

                                                      aaTCGATCGCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAAc                               <  2:1664569/90‑1 (MQ=255)

                                                        tcgatcgCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAg                             <  1:154976/90‑1 (MQ=255)

                                                        tcgatcgCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAg                             >  2:1796785/1‑90 (MQ=255)

                                                         cgatcgCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGc                            >  1:832565/1‑90 (MQ=255)

                                                         cgatcgCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGc                            >  1:1791889/1‑90 (MQ=255)

                                                           atcgCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCAc                          >  1:314381/1‑90 (MQ=255)

                                                           atcgCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGAGACCGCGGCGTTAACAGCAc                          >  1:853474/1‑90 (MQ=255)

                                                            tcgCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACt                         <  1:1857282/90‑1 (MQ=255)

                                                            tcgCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACt                         <  2:383755/90‑1 (MQ=255)

                                                              gCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTa                       >  2:2596106/1‑90 (MQ=255)

                                                              gCATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTa                       <  1:1449085/90‑1 (MQ=255)

                                                               cATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAATTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAg                      >  1:1580917/1‑90 (MQ=255)

                                                               cATAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAg                      <  2:278761/90‑1 (MQ=255)

                                                                aTAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGc                     >  1:193409/1‑90 (MQ=255)

                                                                aTAAAAGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGc                     <  2:2342995/90‑1 (MQ=255)

                                                                aTAAAAGAAGAGAAAGTTCAGCTGCGGCAAAAAGGCGCTGAAGCGCCGGAAAAAATGCGCCGGGCGACCGCGGCGTTAACAGCCCTTAGc                     >  2:2845367/1‑90 (MQ=255)

                                                                   aaaGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGAt                  >  2:834398/1‑90 (MQ=255)

                                                                   aaaGAAGAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGAt                  >  2:2288811/1‑90 (MQ=255)

                                                                    aagaagAGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATg                 >  1:2321752/1‑90 (MQ=255)

                                                                     agaagaGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGt                >  2:2197949/1‑90 (MQ=255)

                                                                     agaagaGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGt                >  1:1302615/1‑90 (MQ=255)

                                                                     agaagaGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGt                >  1:817701/1‑90 (MQ=255)

                                                                      gaagaGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTc               <  1:969182/90‑1 (MQ=255)

                                                                      gaagaGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTc               <  2:256305/90‑1 (MQ=255)

                                                                      gaagaGACAGTTCAGCTGCGGCAAAAAGCCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTc               >  1:749544/1‑90 (MQ=255)

                                                                       aagaGACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCg              >  1:3403485/1‑90 (MQ=255)

                                                                        agagACAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGa             <  2:1897269/90‑1 (MQ=255)

                                                                         gagaCAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGAt            <  2:2832127/90‑1 (MQ=255)

                                                                         gagaCAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGAt            >  1:3157408/1‑90 (MQ=255)

                                                                           gaCAGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATaa          <  1:610256/90‑1 (MQ=255)

                                                                              aGTTCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAacga       >  1:1611212/1‑90 (MQ=255)

                                                                                ttCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAacgacg     <  2:128938/90‑1 (MQ=255)

                                                                                ttCAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAacgacg     <  1:1365633/90‑1 (MQ=255)

                                                                                  cAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAACGACgaa   >  2:3408492/1‑90 (MQ=255)

                                                                                  cAGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAACGACgaa   <  1:2774008/90‑1 (MQ=255)

                                                                                   aGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAACGACgaag  >  2:1671403/1‑90 (MQ=255)

                                                                                   aGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAACGACgaag  <  2:3476627/90‑1 (MQ=255)

                                                                                   aGCTGCGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAACGACgaag  >  1:258943/1‑90 (MQ=255)

TCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAACGACGAAG  >  AC_000091/485945‑486117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`