BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	506,176	A→G	V44A (GTC→GCC)	ybaK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	506,176	0	A	G	100.0%	461.4 / NA	116	V44A (GTC→GCC)	ybaK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (48/68); total (48/68)

Only 100 of 116 total aligned reads displayed.

TTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGATTTGA  >  AC_000091/506089‑506262

ttAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGcc                                                                                      >  1:241604/1‑90 (MQ=255)

ttAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGcc                                                                                      >  1:1674830/1‑90 (MQ=255)

 tAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCt                                                                                     <  1:109386/90‑1 (MQ=255)

 tAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCt                                                                                     <  2:2903569/90‑1 (MQ=255)

 tAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCt                                                                                     <  1:2041532/90‑1 (MQ=255)

  aaGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTg                                                                                    >  1:2555476/1‑90 (MQ=255)

   aGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGa                                                                                   >  1:396033/1‑90 (MQ=255)

    gATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGAt                                                                                  >  1:2241855/1‑90 (MQ=255)

     aTCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATc                                                                                 <  1:2387295/90‑1 (MQ=255)

     aTCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATc                                                                                 <  1:1003172/90‑1 (MQ=255)

     aTCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATc                                                                                 <  1:2137179/90‑1 (MQ=255)

      tCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATcc                                                                                <  1:2238417/90‑1 (MQ=255)

      tCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGATTTGTAGGCCTGATcc                                                                                >  2:266726/1‑90 (MQ=255)

       ccAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCg                                                                               <  1:1693583/90‑1 (MQ=255)

       ccAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCg                                                                               >  1:1861445/1‑90 (MQ=255)

        cAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCgg                                                                              <  2:1998371/90‑1 (MQ=255)

          gTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGAt                                                                            <  1:1271579/90‑1 (MQ=255)

          gTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGAt                                                                            <  2:3342196/90‑1 (MQ=255)

           ttGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGAtt                                                                           <  1:2586307/90‑1 (MQ=255)

             gACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCa                                                                         >  1:1569654/1‑90 (MQ=255)

              aCCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCaa                                                                        >  2:805916/1‑90 (MQ=255)

              aCCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCaa                                                                        <  2:3275577/90‑1 (MQ=255)

              aCCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCaa                                                                        <  1:787684/90‑1 (MQ=255)

                cTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAAc                                                                      >  2:1104622/1‑90 (MQ=255)

                cTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAAc                                                                      >  1:1422545/1‑90 (MQ=255)

                cTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAAc                                                                      <  2:70690/90‑1 (MQ=255)

                  gCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCt                                                                    <  1:920277/90‑1 (MQ=255)

                   cGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTa                                                                   <  1:3229917/90‑1 (MQ=255)

                    gACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCCCCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAGCCTaa                                                                  <  2:3042837/90‑1 (MQ=255)

                    gACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTaa                                                                  <  1:2424302/90‑1 (MQ=255)

                    gACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTaa                                                                  >  1:1299057/1‑90 (MQ=255)

                    gACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTaa                                                                  >  2:2253520/1‑90 (MQ=255)

                     aCCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAAt                                                                 <  1:934580/90‑1 (MQ=255)

                     aCCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAAt                                                                 >  1:1537230/1‑90 (MQ=255)

                      ccGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAAtt                                                                <  2:810205/90‑1 (MQ=255)

                       cGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAAttt                                                               <  2:1627724/90‑1 (MQ=255)

                        ggCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAAtttt                                                              >  1:690798/1‑90 (MQ=255)

                        ggCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAAtttt                                                              <  2:2371202/90‑1 (MQ=255)

                        ggCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAAtttt                                                              <  1:2246493/90‑1 (MQ=255)

                          cGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAAtttttt                                                            <  1:2818880/90‑1 (MQ=255)

                           gTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTg                                                           <  2:2008675/90‑1 (MQ=255)

                            tAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTga                                                          <  2:126393/90‑1 (MQ=255)

                            tAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTga                                                          <  2:521567/90‑1 (MQ=255)

                             aaCGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTgac                                                         >  2:90347/1‑90 (MQ=255)

                               cGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTgacga                                                       <  1:482490/90‑1 (MQ=255)

                                ggCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTgacgac                                                      <  2:1160392/90‑1 (MQ=255)

                                ggCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTgacgac                                                      <  2:2277417/90‑1 (MQ=255)

                                 gcaaCGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACt                                                     >  2:2326070/4‑90 (MQ=255)

                                  ccACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACtt                                                    <  1:1941161/90‑1 (MQ=255)

                                   cACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTc                                                   <  1:558980/90‑1 (MQ=255)

                                    aCGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCa                                                  <  1:955812/90‑1 (MQ=255)

                                     cGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCAt                                                 >  2:2553174/1‑90 (MQ=255)

                                     cGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCAt                                                 <  2:1609937/90‑1 (MQ=255)

                                      ggCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATc                                                >  2:3396981/1‑90 (MQ=255)

                                      ggCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATc                                                >  1:1366265/1‑90 (MQ=255)

                                       gCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCg                                               <  1:449170/90‑1 (MQ=255)

                                       gCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCg                                               <  2:2040268/90‑1 (MQ=255)

                                         aaGGTGTTTCATATCCCCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGcc                                             <  2:3266586/90‑1 (MQ=255)

                                         aaGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGcc                                             <  1:1042953/90‑1 (MQ=255)

                                          aGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCa                                            <  2:601035/90‑1 (MQ=255)

                                          aGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCa                                            <  1:525593/90‑1 (MQ=255)

                                           ggTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCaa                                           >  2:416531/1‑90 (MQ=255)

                                            gtgtTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCaaa                                          >  2:3210775/1‑90 (MQ=255)

                                              gtTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAAt                                        <  1:3227899/90‑1 (MQ=255)

                                               tttCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAAtt                                       <  1:2900513/90‑1 (MQ=255)

                                               tttCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAAtt                                       <  1:2922766/90‑1 (MQ=255)

                                                ttCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTg                                      <  2:2273982/90‑1 (MQ=255)

                                                  cATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGt                                    >  1:479771/1‑90 (MQ=255)

                                                   atatCACAGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTgtgt                                   >  2:2936704/1‑87 (MQ=255)

                                                    tatCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGttt                                  >  1:1998987/1‑90 (MQ=255)

                                                     atCACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTc                                 <  1:3375889/90‑1 (MQ=255)

                                                       cACCGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAg                               <  1:309994/90‑1 (MQ=255)

                                                         ccGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGcc                             >  1:1563761/1‑90 (MQ=255)

                                                          cGTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCg                            >  1:1612422/1‑90 (MQ=255)

                                                           gTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCgg                           >  2:436370/1‑90 (MQ=255)

                                                           gTTCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCgg                           <  2:1649226/90‑1 (MQ=255)

                                                            ttCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGa                          >  1:207585/1‑90 (MQ=255)

                                                            ttCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGa                          >  2:3045915/1‑90 (MQ=255)

                                                             tCACTGCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGAt                         >  2:46459/1‑90 (MQ=255)

                                                                  gCCACCAGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGc                    >  1:2146508/1‑90 (MQ=255)

                                                                    caccaGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTc                  >  2:805704/1‑90 (MQ=255)

                                                                    caccaGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTc                  >  1:858082/1‑90 (MQ=255)

                                                                    caccaGCAGCGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTc                  <  1:2758671/90‑1 (MQ=255)

                                                                         gcagcGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAgg             >  2:499470/1‑90 (MQ=255)

                                                                          cagcGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGt            >  1:2612806/1‑90 (MQ=255)

                                                                          cagcGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGt            <  2:725661/90‑1 (MQ=255)

                                                                          cagcGTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGt            <  1:3260158/90‑1 (MQ=255)

                                                                              gTTTTGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATgg        <  2:3368948/90‑1 (MQ=255)

                                                                               ttttGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGa       >  2:3443851/1‑90 (MQ=255)

                                                                               ttttGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGa       <  2:1746784/90‑1 (MQ=255)

                                                                                tttGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGAt      >  1:3328068/1‑90 (MQ=255)

                                                                                  tGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGAttt    <  2:3153272/90‑1 (MQ=255)

                                                                                  tGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGAttt    >  1:2515999/1‑90 (MQ=255)

                                                                                  tGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGAttt    <  1:3002399/90‑1 (MQ=255)

                                                                                  tGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGAttt    >  1:1859295/1‑90 (MQ=255)

                                                                                  tGTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGAttt    <  1:3268179/90‑1 (MQ=255)

                                                                                   gTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGATTTg   >  2:2740646/1‑90 (MQ=255)

                                                                                   gTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGATTTg   >  2:1364145/1‑90 (MQ=255)

                                                                                   gTAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGATTTg   >  1:486950/1‑90 (MQ=255)

                                                                                    tAGGCCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGCTATGGATTTGa  <  1:1872661/90‑1 (MQ=255)

TTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGATTCAAACCTAATTTTTTGACGACTTCATCGCCAAAATTGGTTTCAGCCGGATCGTGCTCGTAGGTATGGATTTGA  >  AC_000091/506089‑506262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`