BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	737,655	A→G	intergenic (+534/‑859)	ybfQ → / → ybfD	predicted transposase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	737,655	0	A	G	100.0%	364.3 / NA	116	intergenic (+534/‑859)	ybfQ/ybfD	predicted transposase/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (50/66); total (50/66)

Only 100 of 117 total aligned reads displayed.

ACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAA  >  AC_000091/737580‑737741

aCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCggg                                                                          <  1:1112345/90‑1 (MQ=14)

aCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCggg                                                                          >  2:1729245/1‑90 (MQ=14)

aCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGCGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCggg                                                                          <  2:401928/90‑1 (MQ=9)

 cGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGc                                                                         >  2:679896/1‑90 (MQ=17)

 cGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGc                                                                         >  1:3086112/1‑90 (MQ=17)

  gCTCCGGCACTCTTATGACAAGAGTCGCCGCATGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCa                                                                        >  1:3485613/1‑90 (MQ=255)

  gCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCa                                                                        <  2:754975/90‑1 (MQ=18)

  gCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCa                                                                        <  2:646268/90‑1 (MQ=18)

  gCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCa                                                                        >  2:488520/1‑90 (MQ=18)

   cTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAg                                                                       <  1:1271060/90‑1 (MQ=18)

   cTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAg                                                                       >  1:25723/1‑90 (MQ=18)

     ccGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGAt                                                                     >  1:875105/1‑90 (MQ=18)

     ccGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGAt                                                                     >  1:3478025/1‑90 (MQ=18)

      cGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATc                                                                    <  2:3166319/90‑1 (MQ=18)

       ggCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCa                                                                   <  2:1078137/90‑1 (MQ=18)

        gCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCaa                                                                  >  2:105677/1‑90 (MQ=18)

        gCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCaa                                                                  >  2:2547138/1‑90 (MQ=18)

         cACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAg                                                                 >  1:255394/1‑90 (MQ=18)

            tctTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACg                                                              >  1:3356753/1‑90 (MQ=18)

            tctTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACg                                                              <  1:1411867/90‑1 (MQ=18)

              ttATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGa                                                            <  1:88595/90‑1 (MQ=25)

              ttATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGa                                                            >  2:1543927/1‑90 (MQ=18)

               tATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGAt                                                           <  2:1954188/90‑1 (MQ=25)

                 tGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATaa                                                         >  1:3107124/1‑90 (MQ=37)

                 tGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATaa                                                         >  2:3292814/1‑90 (MQ=37)

                 tGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATaa                                                         <  2:3127046/90‑1 (MQ=255)

                 tGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATaa                                                         <  1:1471553/90‑1 (MQ=255)

                  gACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAg                                                        >  2:2859353/1‑90 (MQ=255)

                  gACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAg                                                        >  1:2322806/1‑90 (MQ=255)

                  gACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAg                                                        <  1:1917509/90‑1 (MQ=255)

                    cAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGaa                                                      >  2:2069097/1‑90 (MQ=255)

                    cAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGaa                                                      >  1:2119680/1‑90 (MQ=255)

                    cAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCCGGCAGATCAAGACGGATAAGaa                                                      <  2:2943172/90‑1 (MQ=255)

                    cAAGAGGCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGaa                                                      <  2:1010157/90‑1 (MQ=255)

                      agagTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAAt                                                    >  2:2235084/1‑90 (MQ=255)

                       gagTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATc                                                   <  1:1358685/90‑1 (MQ=255)

                        agTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATcc                                                  <  2:2081127/90‑1 (MQ=255)

                        agTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATcc                                                  <  1:1838022/90‑1 (MQ=255)

                        agTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATcc                                                  >  2:1311323/1‑90 (MQ=255)

                         gTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCa                                                 >  2:2211660/1‑90 (MQ=255)

                          tCGCCGCAGGGGAGCGATTGATGTCATTAGTGCGTTCTCAGCAATGCACGGTCTGGTCATGGGGCAGATCAAGACGGATAAGAAATCCaa                                                >  2:2540524/1‑90 (MQ=14)

                          tCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCaa                                                <  1:311327/90‑1 (MQ=255)

                          tCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCaa                                                <  1:3172583/90‑1 (MQ=255)

                            gccgcAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATg                                              >  1:3076760/1‑90 (MQ=255)

                             ccgcAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATga                                             <  2:954916/90‑1 (MQ=255)

                             ccgcAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATga                                             <  2:971443/90‑1 (MQ=255)

                              cgcAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATgag                                            <  2:886431/90‑1 (MQ=255)

                               gcAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATgaga                                           >  1:2081159/1‑90 (MQ=255)

                               gcAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATgaga                                           >  1:1414092/1‑90 (MQ=255)

                               gcAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATgaga                                           <  2:659006/90‑1 (MQ=255)

                                cAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGTTAAGAAATCCAATGAGAt                                          <  2:2129355/90‑1 (MQ=255)

                                cAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGAt                                          <  1:2216561/90‑1 (MQ=255)

                                cAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGAt                                          <  2:1479697/90‑1 (MQ=255)

                                 aGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATc                                         >  1:1433870/1‑90 (MQ=255)

                                 aGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGGCATCGGGCAGATAAAGACGGATAAGAAACCCAATGAGATc                                         >  2:877061/1‑90 (MQ=11)

                                  ggggAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATca                                        <  2:7121/90‑1 (MQ=25)

                                    ggAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATcaca                                      <  1:2438171/90‑1 (MQ=25)

                                    ggAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATcaca                                      <  1:1212345/90‑1 (MQ=18)

                                     gAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAg                                     <  1:1263368/90‑1 (MQ=25)

                                       gCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCt                                   <  1:979539/90‑1 (MQ=25)

                                       gCGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCt                                   <  1:1568240/90‑1 (MQ=18)

                                        cGATTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTa                                  <  2:1152144/90‑1 (MQ=18)

                                          aTTCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATc                                <  2:3223286/90‑1 (MQ=25)

                                           ttCATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATcc                               >  2:3267708/1‑90 (MQ=25)

                                             cATGTCATTAGTGCGTTCTCAACAATGCACGGTCTTGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCt                             >  1:2794227/1‑90 (MQ=255)

                                             cATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCt                             <  2:3385389/90‑1 (MQ=25)

                                             cATGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCt                             <  1:2605329/90‑1 (MQ=18)

                                              aTGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTg                            <  1:1982551/90‑1 (MQ=25)

                                              aTGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTg                            <  2:3433078/90‑1 (MQ=25)

                                              aTGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTg                            >  2:2991941/1‑90 (MQ=18)

                                               tGTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGa                           <  1:2048637/90‑1 (MQ=25)

                                                gTCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGaa                          >  2:3226672/1‑90 (MQ=25)

                                                 tCATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAAc                         >  1:1069594/1‑90 (MQ=18)

                                                  cATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAAct                        <  2:3430470/90‑1 (MQ=18)

                                                  cATTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAAct                        <  2:2370724/90‑1 (MQ=18)

                                                   aTTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTCTCCCTGAActt                       <  1:2624232/90‑1 (MQ=255)

                                                   aTTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAActt                       <  2:2302592/90‑1 (MQ=25)

                                                   aTTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAActt                       >  1:368816/1‑90 (MQ=25)

                                                   aTTAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAActt                       <  2:1988641/90‑1 (MQ=25)

                                                     tAGTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAActtct                     >  2:1678352/1‑90 (MQ=25)

                                                       gTGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTa                   <  2:1743870/90‑1 (MQ=18)

                                                       gTGCGTTCTCAACAANGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTa                   >  1:1239456/1‑90 (MQ=25)

                                                        tGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTaa                  <  2:905908/90‑1 (MQ=25)

                                                        tGCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTaa                  <  1:818154/90‑1 (MQ=25)

                                                         gCGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAACAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAAc                 <  2:1455649/90‑1 (MQ=255)

                                                          cGTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACa                <  1:2626619/90‑1 (MQ=18)

                                                           gTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACAt               <  1:1202806/90‑1 (MQ=25)

                                                           gTTCTCAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACAt               <  2:719976/90‑1 (MQ=25)

                                                              ctcAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCt            >  2:278132/1‑90 (MQ=25)

                                                              ctcAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCt            <  2:1551804/90‑1 (MQ=25)

                                                               tcAACAATGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTg           <  1:1173374/90‑1 (MQ=25)

                                                                caacaaTGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTgg          <  1:1916656/90‑1 (MQ=25)

                                                                  acaaTGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGat        <  2:416540/90‑1 (MQ=25)

                                                                  acaaTGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGat        <  1:448168/90‑1 (MQ=25)

                                                                   caaTGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGata       >  2:1650194/1‑90 (MQ=25)

                                                                    aaTGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGatat      <  2:2339044/90‑1 (MQ=25)

                                                                     aTGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATAtt     >  2:107449/1‑90 (MQ=25)

                                                                      tGCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTa    <  1:3445277/90‑1 (MQ=25)

                                                                       gCACGGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTaa   >  1:99853/1‑90 (MQ=18)

                                                                        cacgGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTaaa  <  1:2160983/90‑1 (MQ=25)

ACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAA  >  AC_000091/737580‑737741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`