BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	30,387	C→T	A246V (GCC→GTC)	carA →	carbamoyl phosphate synthetase small subunit, glutamine amidotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	30,387	0	C	T	100.0%	526.4 / NA	134	A246V (GCC→GTC)	carA	carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (81/53); total (81/53)

Only 100 of 136 total aligned reads displayed.

ATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGC  >  AC_000091/30302‑30473

aTCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGtccca                                                                                    <  2:2025726/90‑2 (MQ=255)

aTCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCg                                                                                    <  1:1417634/90‑1 (MQ=255)

  cGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTg                                                                                  >  2:2891062/1‑90 (MQ=255)

  cGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTg                                                                                  <  2:1987753/90‑1 (MQ=255)

  cGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTg                                                                                  >  2:1898669/1‑90 (MQ=255)

   gTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGc                                                                                 <  1:3077770/90‑1 (MQ=255)

   gTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGc                                                                                 <  1:1362719/90‑1 (MQ=255)

   gTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGc                                                                                 >  2:351107/1‑90 (MQ=255)

       cGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGAtt                                                                             >  2:905211/1‑90 (MQ=255)

         gcgcAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTAc                                                                           >  1:2733883/1‑90 (MQ=255)

            cAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGcc                                                                        <  1:1072325/90‑1 (MQ=255)

            cAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGcc                                                                        >  1:3359637/1‑90 (MQ=255)

              aaCTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCAt                                                                      >  1:401268/1‑90 (MQ=255)

               aCTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGGCCCGGTCCCGTGCGATTACGCCAtt                                                                     <  2:3220719/90‑1 (MQ=255)

               aCTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCAtt                                                                     >  2:2865367/1‑90 (MQ=255)

               aCTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCAtt                                                                     >  2:2970853/1‑90 (MQ=255)

                cTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTa                                                                    >  2:1508971/1‑90 (MQ=255)

                 ttCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTAc                                                                   >  1:1244598/1‑90 (MQ=255)

                 ttCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTAc                                                                   >  2:972126/1‑90 (MQ=255)

                  tCTGCGGCAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTAcc                                                                  <  2:36370/90‑1 (MQ=255)

                  tCTGCGGAAGGTGTGCTGGAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTAcc                                                                  >  2:3325519/1‑90 (MQ=255)

                  tCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTAcc                                                                  >  2:1306171/1‑90 (MQ=255)

                    tGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGc                                                                >  2:1744150/1‑90 (MQ=255)

                    tGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGc                                                                >  2:311037/1‑90 (MQ=255)

                    tGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGc                                                                >  2:2364667/1‑90 (MQ=255)

                     gCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGcc                                                               <  1:2183663/90‑1 (MQ=255)

                     gCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGcc                                                               >  1:2303278/1‑90 (MQ=255)

                      cGGAAGATGTGCTGAAAATGAATCCAGTCGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCa                                                              <  2:3357958/90‑1 (MQ=255)

                      cGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCa                                                              >  1:3177066/1‑90 (MQ=255)

                      cGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCa                                                              <  2:2455138/90‑1 (MQ=255)

                      cGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCa                                                              <  2:2973282/90‑1 (MQ=255)

                       ggAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCAt                                                             >  1:338575/1‑90 (MQ=255)

                       ggAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCAt                                                             >  1:3111979/1‑90 (MQ=255)

                         aaGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATcc                                                           >  2:311193/1‑90 (MQ=255)

                           gATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAg                                                         <  2:1321280/90‑1 (MQ=255)

                            aTGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGa                                                        <  1:3464806/90‑1 (MQ=255)

                                gCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAAtt                                                    <  2:1203147/90‑1 (MQ=255)

                                 cTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTc                                                   <  1:1980253/90‑1 (MQ=255)

                                 cTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTc                                                   >  2:2868407/1‑90 (MQ=255)

                                  tGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTcc                                                  >  2:2481113/1‑90 (MQ=255)

                                  tGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTcc                                                  >  2:2865965/1‑90 (MQ=255)

                                    aaaaTGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTc                                                <  2:533774/90‑1 (MQ=255)

                                    aaaaTGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTc                                                <  1:3143828/90‑1 (MQ=255)

                                     aaaTGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCg                                               <  1:2352633/90‑1 (MQ=255)

                                     aaaTGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCg                                               <  2:2713833/90‑1 (MQ=255)

                                      aaTGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGa                                              >  2:2130563/1‑90 (MQ=255)

                                      aaTGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGa                                              <  1:935359/90‑1 (MQ=255)

                                       aTGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGaa                                             <  1:738996/90‑1 (MQ=255)

                                       aTGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGaa                                             >  1:1551727/1‑90 (MQ=255)

                                       aTGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGaa                                             >  2:503458/1‑90 (MQ=255)

                                         gAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAAc                                           >  2:1433154/1‑90 (MQ=255)

                                         gAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAAc                                           <  2:85182/90‑1 (MQ=255)

                                          aaTCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAAcc                                          >  1:1094869/1‑90 (MQ=255)

                                          aaTCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAAcc                                          >  1:1109268/1‑90 (MQ=255)

                                          aaTCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAAcc                                          <  1:1418200/90‑1 (MQ=255)

                                          aaTCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAAcc                                          >  1:44809/1‑90 (MQ=255)

                                           aTCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCg                                         >  1:2508339/1‑90 (MQ=255)

                                           aTCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCg                                         >  2:3225781/1‑90 (MQ=255)

                                           aTCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCg                                         >  1:684992/1‑90 (MQ=255)

                                           aTCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCg                                         >  1:1644048/1‑90 (MQ=255)

                                           aTCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCg                                         >  1:3195082/1‑90 (MQ=255)

                                           aTCCAGACGGCATCTTCCTCTCCAACGGTACTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCg                                         <  1:2289605/90‑1 (MQ=255)

                                             ccAGACGGCATCTTCCTGTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGat                                       >  2:2566582/1‑90 (MQ=255)

                                              cAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGata                                      >  2:2196596/1‑90 (MQ=255)

                                               aGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGatat                                     <  1:2746424/90‑1 (MQ=255)

                                               aGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGatat                                     >  1:3070859/1‑90 (MQ=255)

                                               aGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGatat                                     >  2:698339/1‑90 (MQ=255)

                                               aGACGGCATCTTCCTCTCAAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGatat                                     >  2:1288394/1‑90 (MQ=255)

                                                   ggCATCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCg                                 <  1:212743/90‑1 (MQ=255)

                                                      aTCTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTa                              <  1:966802/90‑1 (MQ=255)

                                                        cTTCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTAtt                            >  1:1912161/1‑90 (MQ=255)

                                                         ttCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTc                           >  1:906257/1‑90 (MQ=255)

                                                          tcctccCCAACGGTCCTGGCGACCCGGTCCCGCGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCg                          <  1:3151283/90‑1 (MQ=255)

                                                          tCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCg                          >  2:2210805/1‑90 (MQ=255)

                                                          tCCTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCg                          >  1:1860060/1‑90 (MQ=255)

                                                           ccTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCgg                         >  1:1432365/1‑90 (MQ=255)

                                                           ccTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCgg                         >  2:903971/1‑90 (MQ=255)

                                                           ccTCTCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCgg                         >  1:2937559/1‑90 (MQ=255)

                                                            ctctCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCNNNATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGc                        >  2:1961251/1‑90 (MQ=255)

                                                            ctctCCAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGc                        >  2:1186657/1‑90 (MQ=255)

                                                                ccAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCt                    <  2:2152762/90‑1 (MQ=255)

                                                                 cAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTg                   <  1:125487/90‑1 (MQ=255)

                                                                 cAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTg                   <  2:1612317/90‑1 (MQ=255)

                                                                 cAACGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTg                   <  2:219944/90‑1 (MQ=255)

                                                                  aaCGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGt                  <  1:3133174/90‑1 (MQ=255)

                                                                   aCGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGtc                 >  2:2586875/1‑90 (MQ=255)

                                                                    cGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGtct                <  1:3000605/90‑1 (MQ=255)

                                                                    cGGTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGtct                >  1:1762619/1‑90 (MQ=255)

                                                                     ggTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGtctc               <  2:49178/90‑1 (MQ=255)

                                                                      gTCCTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCg              <  1:1813379/90‑1 (MQ=255)

                                                                        ccTGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGt            >  1:751050/1‑90 (MQ=255)

                                                                          tGGCGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGtca          >  1:2099551/1‑90 (MQ=255)

                                                                           ggCGACCCGGTCCCGTGCGATTTCGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGtcat         <  1:2670253/90‑1 (MQ=255)

                                                                             cGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGtcatca       <  2:2667144/90‑1 (MQ=255)

                                                                             cGACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGtcatca       >  2:1474049/1‑90 (MQ=255)

                                                                              gACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAg      >  2:369502/1‑90 (MQ=255)

                                                                              gACCCGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAg      >  1:1531977/1‑90 (MQ=255)

                                                                                cccGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAgct    <  2:2530485/90‑1 (MQ=255)

                                                                                 ccGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAgctg   <  2:3265421/90‑1 (MQ=255)

                                                                                  cGGTCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACNNATNTTCCGGTATTCGGCATCTGTCTCGGTCATCAgctgc  >  2:31685/1‑90 (MQ=255)

ATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGC  >  AC_000091/30302‑30473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`