Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	778,483	C→T	P108S (CCG→TCG)	tolB →	periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	778,483	0	C	T	100.0%	392.6 / NA	96	P108S (CCG→TCG)	tolB	periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/49);  total (47/49)

CAGCCGGGTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTG  >  AC_000091/778396‑778569                                                                                        |                                                                                       cagcCGGGTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCCCTCCGAATTCg                                                                                      <  2:1156790/90‑1 (MQ=255) cagcCGGGTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCg                                                                                      >  2:1666795/1‑90 (MQ=255) cagcCGGGTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCg                                                                                      >  2:2971480/1‑90 (MQ=255)  agcCGGGTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCgg                                                                                     >  2:278277/1‑90 (MQ=255)  agcCGGGTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCgg                                                                                     >  2:3168771/1‑90 (MQ=255)    ccGGGTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGAt                                                                                   <  2:1804145/90‑1 (MQ=255)     cGGGTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATNGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATg                                                                                  >  2:413817/1‑90 (MQ=255)      gggTAGTGGGCAGGAAGTACAACGAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATgg                                                                                 >  2:1886232/1‑90 (MQ=255)      gggTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATgg                                                                                 <  2:2256360/90‑1 (MQ=255)       ggTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGt                                                                                <  1:1193125/90‑1 (MQ=255)         tAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTc                                                                              <  2:573578/90‑1 (MQ=255)          aGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATTGTTCt                                                                             <  2:2298498/90‑1 (MQ=255)          aGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCt                                                                             <  2:2421854/90‑1 (MQ=255)          aGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCt                                                                             <  2:1880728/90‑1 (MQ=255)          aGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGGAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCt                                                                             <  2:2752371/90‑1 (MQ=255)           gTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCtt                                                                            <  1:1662087/90‑1 (MQ=255)           gTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCtt                                                                            <  2:278721/90‑1 (MQ=255)              cgcAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACa                                                                         <  1:533351/90‑1 (MQ=255)                cAGGCAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAAt                                                                       <  1:867128/90‑1 (MQ=255)                cAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAAt                                                                       <  1:410164/90‑1 (MQ=255)                cAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAAt                                                                       <  2:1404926/90‑1 (MQ=255)                 aGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATg                                                                      >  2:3113306/1‑90 (MQ=255)                  ggAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGt                                                                     >  2:2889803/1‑90 (MQ=255)                   gAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGtt                                                                    >  1:775785/1‑90 (MQ=255)                   gAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGtt                                                                    >  2:2563798/1‑90 (MQ=255)                   gAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGtt                                                                    >  2:2463358/1‑90 (MQ=255)                      gTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCt                                                                 >  2:2443113/1‑90 (MQ=255)                         cAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTAt                                                              <  1:1378058/90‑1 (MQ=255)                         cAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTAt                                                              <  1:564817/90‑1 (MQ=255)                         cAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTAt                                                              <  2:1508229/90‑1 (MQ=255)                          aaCCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATc                                                             >  1:2973983/1‑90 (MQ=255)                           aCCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCa                                                            >  2:740421/1‑90 (MQ=255)                           aCCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCa                                                            >  2:2792051/1‑90 (MQ=255)                            ccAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCaa                                                           <  1:2644991/90‑1 (MQ=255)                             cAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAAc                                                          >  1:297470/1‑90 (MQ=255)                             cAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAAc                                                          >  2:2721645/1‑90 (MQ=255)                              aacTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGATGCTTATCAACt                                                         >  2:1792848/3‑90 (MQ=255)                              aGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACt                                                         <  1:292069/90‑1 (MQ=255)                               gCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCCCTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACtt                                                        <  1:3427356/90‑1 (MQ=255)                                cTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACttg                                                       <  2:3341253/90‑1 (MQ=255)                                  gCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACttgtt                                                     <  1:187292/90‑1 (MQ=255)                                  gCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACttgtt                                                     <  2:2687527/90‑1 (MQ=255)                                   cATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACttgttg                                                    >  1:3203976/1‑90 (MQ=255)                                   cATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACttgttg                                                    <  1:1043214/90‑1 (MQ=255)                                     tGGTCCGCACTTGGCATTGACGCTGTAGTTGTCGGTCAGGTCTCTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGac                                                  <  2:3005880/90‑1 (MQ=255)                                     tGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGac                                                  >  1:180561/1‑90 (MQ=255)                                       gTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGacac                                                >  1:1258659/1‑90 (MQ=255)                                        tCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACt                                               <  1:413408/90‑1 (MQ=255)                                         ccGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTg                                              <  1:614217/90‑1 (MQ=255)                                             aCTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTggcgg                                          >  1:653645/1‑90 (MQ=255)                                             aCTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTggcgg                                          <  1:2628483/90‑1 (MQ=255)                                              cTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTggcggc                                         <  1:3122248/90‑1 (MQ=255)                                              cTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTggcggc                                         <  1:1653078/90‑1 (MQ=255)                                               tGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGgcggcg                                        >  1:2784006/1‑90 (MQ=255)                                                 ggCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGGGCa                                      >  2:3251136/1‑90 (MQ=255)                                                 ggCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCa                                      <  2:406319/90‑1 (MQ=255)                                                 ggCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCa                                      <  2:3361079/90‑1 (MQ=255)                                                    aTTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCg                                   <  2:3264979/90‑1 (MQ=255)                                                    aTTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCg                                   <  2:2865015/90‑1 (MQ=255)                                                    aTTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCg                                   >  2:3392273/1‑90 (MQ=255)                                                    aTTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACAGGCGGCGCACCg                                   >  2:985522/1‑90 (MQ=255)                                                      tGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCggg                                 <  2:1674327/90‑1 (MQ=255)                                                      tGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCggg                                 >  2:872440/1‑90 (MQ=255)                                                        aCGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGgta                               >  2:1579709/1‑90 (MQ=255)                                                         cGCTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGgtac                              >  2:1195601/1‑90 (MQ=255)                                                           cTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGgtactg                            <  1:195090/90‑1 (MQ=255)                                                           cTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGgtactg                            >  2:1169073/1‑90 (MQ=255)                                                           cTGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGgtactg                            >  2:397183/1‑90 (MQ=255)                                                            tGTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGgtactgt                           <  1:2595871/90‑1 (MQ=255)                                                             gTAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGgtactgta                          >  1:1815759/1‑90 (MQ=255)                                                             gTAGGTGTCGGTCAGGTCTCTCCGAATTCGGAAGGTTCTTACAATGTTGCTTAGCCACTTGTTGACACTGGCGGCGCACCGGgtactgta                          <  2:845552/90‑1 (MQ=255)                                                              tAGTTGTCGGTCAGGTCACTCCGAATTCGGATGGCTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGgtactgtac                         >  2:188063/1‑90 (MQ=255)                                                               aGTTGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGgtactgtact                        <  2:150366/90‑1 (MQ=255)                                                                 ttGTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTg                      >  1:2195083/1‑90 (MQ=255)                                                                   gTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCt                    <  2:1281179/90‑1 (MQ=255)                                                                   gTCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCt                    <  1:2883104/90‑1 (MQ=255)                                                                    tCGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTc                   <  1:647903/90‑1 (MQ=255)                                                                     cGGTCAGGTCACTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCa                  >  2:1669766/1‑90 (MQ=255)                                                                       gtcaggtcaCTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGa                <  1:1412848/90‑1 (MQ=255)                                                                       gtcaggtcaCTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGa                <  1:1760358/90‑1 (MQ=255)                                                                        tcaggtcaCTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGaa               >  1:3456063/1‑90 (MQ=255)                                                                         caggtcaCTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAAc              >  1:2289549/1‑90 (MQ=255)                                                                          aggtcaCTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACt             <  1:82856/90‑1 (MQ=255)                                                                             tcaCTCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTTGTGACACTGGCGGCGCACCGGGGACGGGACTTGCTCAGAACTCGt          >  2:240884/1‑90 (MQ=255)                                                                                 tCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTg                      >  1:1170241/1‑74 (MQ=255)                                                                                 tCCGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTg                      <  2:1170241/74‑1 (MQ=255)                                                                                  ccGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACaaa     >  2:1270857/1‑90 (MQ=255)                                                                                  ccGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACaaa     >  1:2314944/1‑90 (MQ=255)                                                                                   cGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAg    <  2:68304/90‑1 (MQ=255)                                                                                   cGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAg    >  1:2436801/1‑90 (MQ=255)                                                                                   cGAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAg    <  1:1955591/90‑1 (MQ=255)                                                                                    gAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGt   >  1:371711/1‑90 (MQ=255)                                                                                    gAATTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGGACTGTACTTGCTCAGAACTCGTACAAAGt   >  2:1905180/1‑90 (MQ=255)                                                                                     aaTTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTg  <  2:2932010/90‑1 (MQ=255)                                                                                     aaTTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTg  >  1:3396929/1‑90 (MQ=255)                                                                                     aaTTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTg  >  1:2609143/1‑90 (MQ=255)                                                                                     aaTTCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTg  >  1:227693/1‑90 (MQ=255)                                                                                        |                                                                                       CAGCCGGGTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTG  >  AC_000091/778396‑778569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑