BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	962,866	A→G	K150K (AAA→AAG)	rpsA →	30S ribosomal subunit protein S1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	962,866	0	A	G	100.0%	440.0 / NA	109	K150K (AAA→AAG)	rpsA	30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (44/65); total (44/65)

Only 100 of 119 total aligned reads displayed.

TGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAATCCGAAAACAG  >  AC_000091/962779‑962952

tGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAgga                                                                                      >  1:1232766/1‑90 (MQ=255)

tGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAgga                                                                                      <  1:2369783/90‑1 (MQ=255)

tGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAgga                                                                                      >  1:2647778/1‑90 (MQ=255)

 gTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAggag                                                                                     >  1:1700889/1‑90 (MQ=255)

 gTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAggag                                                                                     >  2:2825439/1‑90 (MQ=255)

 gTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAggag                                                                                     >  2:2810734/1‑90 (MQ=255)

 gTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAggag                                                                                     <  2:1939050/90‑1 (MQ=255)

 gTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAggag                                                                                     >  2:1249136/1‑90 (MQ=255)

  ttGAGCTTAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGc                                                                                    <  2:727270/90‑1 (MQ=255)

   tGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCt                                                                                   <  1:257135/90‑1 (MQ=255)

   tGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCt                                                                                   <  1:829251/90‑1 (MQ=255)

    gAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCtt                                                                                  <  1:1472156/90‑1 (MQ=255)

     aGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTg                                                                                 <  2:1346812/90‑1 (MQ=255)

     aGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTg                                                                                 <  1:741827/90‑1 (MQ=255)

       cTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGaa                                                                               >  2:906363/1‑90 (MQ=255)

       cTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGaa                                                                               <  2:2477905/90‑1 (MQ=255)

       cTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGaa                                                                               >  1:2635973/1‑90 (MQ=255)

       cTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGaa                                                                               <  2:766765/90‑1 (MQ=255)

         gAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAAtt                                                                             <  2:391740/90‑1 (MQ=255)

          aaCGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAAttt                                                                            >  2:2652482/1‑90 (MQ=255)

          aaCGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAAttt                                                                            <  2:3334786/90‑1 (MQ=255)

          aaCGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAAttt                                                                            >  1:3007166/1‑90 (MQ=255)

          aaCGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAAttt                                                                            >  2:2370267/1‑90 (MQ=255)

            cGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTaa                                                                          <  1:208994/90‑1 (MQ=255)

              gTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGCATTTAAAg                                                                        >  2:1288955/1‑90 (MQ=255)

              gTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAg                                                                        >  2:2574526/1‑90 (MQ=255)

               tATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGt                                                                       >  1:419355/1‑90 (MQ=255)

               tATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGt                                                                       <  2:775661/90‑1 (MQ=255)

                 ttCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTaa                                                                     <  2:1965663/90‑1 (MQ=255)

                     tGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCaa                                                                 <  2:2289702/90‑1 (MQ=255)

                     tGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCaa                                                                 <  2:331210/90‑1 (MQ=255)

                     tGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCaa                                                                 >  1:2472530/1‑90 (MQ=255)

                      gCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAg                                                                <  1:428752/90‑1 (MQ=255)

                       cGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGc                                                               <  1:144533/90‑1 (MQ=255)

                       cGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGc                                                               <  1:174913/90‑1 (MQ=255)

                       cGTTCCTGCCAGGTTCTCTCGTAGACGTTCGTCCGGTGCGTGACACCCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGc                                                               <  1:794696/90‑1 (MQ=255)

                         ttCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTg                                                             <  2:3067664/90‑1 (MQ=255)

                         ttCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTg                                                             <  1:3340823/90‑1 (MQ=255)

                           ccTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGa                                                           <  1:2199081/90‑1 (MQ=255)

                           ccTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGa                                                           >  2:89173/1‑90 (MQ=255)

                            cTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGAt                                                          >  2:2440507/1‑90 (MQ=255)

                               ccAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAg                                                       >  1:1587391/1‑90 (MQ=255)

                               ccAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAg                                                       >  2:413915/1‑90 (MQ=255)

                                cAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGa                                                      >  2:2354848/1‑90 (MQ=255)

                                cAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGa                                                      >  1:1941415/1‑90 (MQ=255)

                                cAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGa                                                      >  1:1795090/1‑90 (MQ=255)

                                 aGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGaa                                                     >  2:2206170/1‑90 (MQ=255)

                                 aGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGaa                                                     <  1:161315/90‑1 (MQ=255)

                                  ggTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAg                                                    <  1:2143046/90‑1 (MQ=255)

                                   gTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAgc                                                   <  1:2400081/90‑1 (MQ=255)

                                   gTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAgc                                                   <  2:1781888/90‑1 (MQ=255)

                                    ttCTCTGGTAGACGTCCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAgcg                                                  <  2:2861551/90‑1 (MQ=255)

                                     tctcTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAgcgc                                                 >  2:46449/1‑90 (MQ=255)

                                      ctctGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGca                                                <  1:1981769/90‑1 (MQ=255)

                                       tctGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGcaa                                               <  1:1105627/90‑1 (MQ=255)

                                        ctGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGcaac                                              <  1:377610/90‑1 (MQ=255)

                                            tAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACg                                          >  2:2152/1‑90 (MQ=255)

                                              gACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgtt                                        <  1:2467578/90‑1 (MQ=255)

                                                 gTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgtt                                     <  1:2361893/90‑1 (MQ=255)

                                                  ttCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgttg                                    <  1:2885717/90‑1 (MQ=255)

                                                   tCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgttgt                                   <  1:2125130/90‑1 (MQ=255)

                                                     gTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGttt                                 <  1:627167/90‑1 (MQ=255)

                                                     gTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGttt                                 >  2:1045887/1‑90 (MQ=255)

                                                     gTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGttt                                 <  1:2227834/90‑1 (MQ=255)

                                                      tCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtc                                <  2:1360824/90‑1 (MQ=255)

                                                      tCCGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtc                                >  1:790670/1‑90 (MQ=255)

                                                        cGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtctc                              >  2:364134/1‑90 (MQ=255)

                                                        cGGTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtctc                              >  1:374884/1‑90 (MQ=255)

                                                          gTGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCtcgt                            <  1:3203811/90‑1 (MQ=255)

                                                           tGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACATCGTTGTTGTTTCtcgtc                           <  2:3316757/90‑1 (MQ=255)

                                                           tGCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCtcgtc                           <  2:1593351/90‑1 (MQ=255)

                                                            gCGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCtcgtcg                          >  2:2656628/1‑90 (MQ=255)

                                                             cGTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTcgtcgt                         <  2:2046706/90‑1 (MQ=255)

                                                              gTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTg                        >  1:3378626/1‑90 (MQ=255)

                                                              gTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTg                        <  1:2689380/90‑1 (MQ=255)

                                                              gTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTg                        <  2:1058931/90‑1 (MQ=255)

                                                              gTGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTg                        >  1:2361775/1‑90 (MQ=255)

                                                               tGACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGc                       <  2:1884582/90‑1 (MQ=255)

                                                                gACACTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGcc                      >  1:2350008/1‑90 (MQ=255)

                                                                 acacTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCg                     <  1:2472160/90‑1 (MQ=255)

                                                                  cacTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGt                    <  1:2220995/90‑1 (MQ=255)

                                                                   acTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGtt                   <  2:1596974/90‑1 (MQ=255)

                                                                   acTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGtt                   <  1:2008855/90‑1 (MQ=255)

                                                                   acTCTGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGtt                   <  1:1622048/90‑1 (MQ=255)

                                                                    ctctGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTa                  <  1:2696508/90‑1 (MQ=255)

                                                                    ctctGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTa                  >  2:2910850/1‑90 (MQ=255)

                                                                      ctGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATc                <  1:2162240/90‑1 (MQ=255)

                                                                      ctGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATc                <  2:469890/90‑1 (MQ=255)

                                                                       tGCACCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCg               >  1:88182/1‑90 (MQ=255)

                                                                          aCCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAAt            >  1:3296094/1‑90 (MQ=255)

                                                                          aCCTGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAAt            >  1:2112460/1‑90 (MQ=255)

                                                                             tGGAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAATCCg         >  1:498608/1‑90 (MQ=255)

                                                                              ggAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAATCCGa        <  1:1914907/90‑1 (MQ=255)

                                                                               gAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAATCCGaa       <  1:3130199/90‑1 (MQ=255)

                                                                               gAAGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTATCGTCTTGCCGTTATCGAATCCGaa       >  1:1853776/1‑90 (MQ=255)

                                                                                 aGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAATCCGaaaa     >  1:3226972/1‑90 (MQ=255)

                                                                                 aGGCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAATCCGaaaa     >  1:1011532/1‑90 (MQ=255)

                                                                                  ggCAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAATCCGAAAAc    >  2:389102/1‑90 (MQ=255)

                                                                                    cAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAATCCGAAAACAg  <  1:3115577/90‑1 (MQ=255)

                                                                                    cAAGGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAATCCGAAAACAg  <  1:451751/90‑1 (MQ=255)

TGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTCGTGCCGTTATCGAATCCGAAAACAG  >  AC_000091/962779‑962952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`