BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,045,186	A→G	L347P (CTG→CCG)	yccZ ←	predicted exopolysaccharide export protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,045,186	0	A	G	100.0%	506.4 / NA	127	L347P (CTG→CCG)	yccZ	predicted exopolysaccharide export protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (56/71); total (56/71)

Only 100 of 131 total aligned reads displayed.

GTTTGAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTC  >  AC_000091/1045099‑1045272

 tttGAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGgcg                                                                                     >  1:594344/1‑90 (MQ=255)

  ttGAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCcggcgg                                                                                    >  1:638723/1‑90 (MQ=255)

    gAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGgcggcg                                                                                  <  1:2892981/90‑1 (MQ=255)

     aaTCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGgcgc                                                                                 >  1:2677815/1‑90 (MQ=255)

     aaTCCACCGTGAAGTCTCTATCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGgcgc                                                                                 <  1:46124/90‑1 (MQ=255)

       tCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCgg                                                                               >  2:1097761/1‑90 (MQ=255)

         cACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTa                                                                             <  2:32841/90‑1 (MQ=255)

         cACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTa                                                                             >  1:3255671/1‑90 (MQ=255)

         cACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTa                                                                             >  1:930505/1‑90 (MQ=255)

          aCCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAg                                                                            >  1:1332256/1‑90 (MQ=255)

           ccGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGt                                                                           <  2:1297510/90‑1 (MQ=255)

           ccGTGAAGTCGCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGt                                                                           <  2:850705/90‑1 (MQ=255)

            cGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTg                                                                          >  1:1434984/1‑90 (MQ=255)

             gTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGa                                                                         <  2:585502/90‑1 (MQ=255)

             gTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGa                                                                         <  2:2419148/90‑1 (MQ=255)

              tGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGAc                                                                        >  2:1003864/1‑90 (MQ=255)

              tGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGAc                                                                        <  1:2085068/90‑1 (MQ=255)

              tGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGAc                                                                        <  1:2073578/90‑1 (MQ=255)

               gAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACa                                                                       >  2:52070/1‑90 (MQ=255)

               gAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACa                                                                       <  1:1895653/90‑1 (MQ=255)

                aaGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTCCCAACGAGCCGGCGGCGCGGTAGTGACAt                                                                      <  2:1133361/90‑1 (MQ=255)

                 aGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATa                                                                     >  1:972618/1‑90 (MQ=255)

                  gTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATaa                                                                    <  1:946260/90‑1 (MQ=255)

                  gTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATaa                                                                    >  2:2310643/1‑90 (MQ=255)

                      ctGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACAAGCCGGCGGCGCGGTAGTGACATAAACaa                                                                >  1:1738529/1‑90 (MQ=255)

                       tGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAAt                                                               >  2:852965/1‑90 (MQ=255)

                        gTCAGGTCATGCACCCCGGAAATCGTCGGTCCCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATg                                                              <  2:3201355/90‑1 (MQ=255)

                        gTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATg                                                              <  1:1366789/90‑1 (MQ=255)

                        gTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATg                                                              <  1:1057291/90‑1 (MQ=255)

                          cAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTc                                                            <  2:1491133/90‑1 (MQ=255)

                          cAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTc                                                            >  1:1033432/1‑90 (MQ=255)

                           aGGTCATGCCCCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCg                                                           <  1:3254446/90‑1 (MQ=255)

                           aGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCg                                                           >  1:1331679/1‑90 (MQ=255)

                           aGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCg                                                           >  2:1154510/1‑90 (MQ=255)

                             gTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTa                                                         >  2:1003283/1‑90 (MQ=255)

                             gTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTa                                                         <  1:2831305/90‑1 (MQ=255)

                              tCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGATCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGaaa                                                        >  2:625873/1‑90 (MQ=255)

                               cATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAg                                                       >  1:3357095/1‑90 (MQ=255)

                                aTGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAgg                                                      >  1:2657279/1‑90 (MQ=255)

                                     ccccGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCaa                                                 <  1:1585092/90‑1 (MQ=255)

                                      cccGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAg                                                >  2:24370/1‑90 (MQ=255)

                                       ccGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGc                                               <  1:568692/90‑1 (MQ=255)

                                        cGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCt                                              <  2:1119457/90‑1 (MQ=255)

                                        cGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCt                                              <  1:2214077/90‑1 (MQ=255)

                                         ggAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTg                                             >  2:2002874/1‑90 (MQ=255)

                                          gAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTgg                                            <  1:731472/90‑1 (MQ=255)

                                              tcgtcgGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAtt                                        >  2:3213170/1‑90 (MQ=255)

                                              tcgtcgGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAtt                                        >  2:3416909/1‑90 (MQ=255)

                                              tcgtcgGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAtt                                        <  2:2724205/90‑1 (MQ=255)

                                               cgtcgGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAttc                                       >  1:3152374/1‑90 (MQ=255)

                                                gtcgGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAttcc                                      >  2:1596339/1‑90 (MQ=255)

                                                gtcgGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAttcc                                      <  1:947845/90‑1 (MQ=255)

                                                gtcgGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAttcc                                      <  2:2846405/90‑1 (MQ=255)

                                                 tcgGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAttccg                                     <  2:345304/90‑1 (MQ=255)

                                                   ggTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                   <  1:1187525/90‑1 (MQ=255)

                                                    gTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAttccgttc                                  >  2:2239766/1‑90 (MQ=255)

                                                    gTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAttccgttc                                  <  2:918852/90‑1 (MQ=255)

                                                    gTACCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAttccgttc                                  >  1:479734/1‑90 (MQ=255)

                                                      aCCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAttccgttccg                                >  2:1192891/1‑90 (MQ=255)

                                                      aCCAGCAGAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAAttccgttccg                                <  1:1250260/90‑1 (MQ=255)

                                                           cagAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTGGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTAt                           >  1:3056306/1‑90 (MQ=255)

                                                           cagAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTAt                           <  1:185039/90‑1 (MQ=255)

                                                           cagAGAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTAt                           >  2:1185739/1‑90 (MQ=255)

                                                            agagAAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATc                          >  1:1149313/1‑90 (MQ=255)

                                                              agaAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCAt                        <  1:3421195/90‑1 (MQ=255)

                                                              agaAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCAt                        <  1:266621/90‑1 (MQ=255)

                                                               gaAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCAtt                       <  1:2450020/90‑1 (MQ=255)

                                                               gaAATCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCAtt                       <  1:1691016/90‑1 (MQ=255)

                                                                aaaTCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTg                      <  2:2808981/90‑1 (MQ=255)

                                                                aaaTCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTg                      <  1:107137/90‑1 (MQ=255)

                                                                   tCACACGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCg                   >  2:2099819/1‑90 (MQ=255)

                                                                    cacaCGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGa                  <  1:1974800/90‑1 (MQ=255)

                                                                      cacGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATg                <  1:3081650/90‑1 (MQ=255)

                                                                      cacGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATg                >  1:2523947/1‑90 (MQ=255)

                                                                       acGGTTCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGc               <  2:29411/90‑1 (MQ=255)

                                                                           ttCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTcc           <  2:2505544/90‑1 (MQ=255)

                                                                           ttCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGGTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTcc           >  2:1695644/1‑90 (MQ=255)

                                                                            tCCAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCt          >  1:1047239/1‑90 (MQ=255)

                                                                             ccAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCtt         <  1:1207969/90‑1 (MQ=255)

                                                                             ccAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCtt         <  2:280514/90‑1 (MQ=255)

                                                                             ccAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCtt         <  2:2931876/90‑1 (MQ=255)

                                                                              cAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTc        <  2:707582/90‑1 (MQ=255)

                                                                              cAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTc        <  1:744109/90‑1 (MQ=255)

                                                                              cAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTc        <  1:3259313/90‑1 (MQ=255)

                                                                              cAACGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTc        >  1:1440538/1‑90 (MQ=255)

                                                                               aaCGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCg       >  2:2730472/1‑90 (MQ=255)

                                                                               aaCGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCg       >  1:837347/1‑90 (MQ=255)

                                                                               aaCGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCg       <  2:2664609/90‑1 (MQ=255)

                                                                               aaCGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCg       <  1:2260835/90‑1 (MQ=255)

                                                                               aaCGAGCCGGCGGCGCGGTAGTGACATAAACAATGGCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCg       >  1:2400400/1‑90 (MQ=255)

                                                                                 cGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCa     <  1:2768641/90‑1 (MQ=255)

                                                                                 cGAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCa     <  1:1379632/90‑1 (MQ=255)

                                                                                  gAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCAt    >  2:3473857/1‑90 (MQ=255)

                                                                                  gAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCAt    >  1:167097/1‑90 (MQ=255)

                                                                                  gAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCAt    >  1:1602032/1‑90 (MQ=255)

                                                                                  gAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCAt    >  2:3253640/1‑90 (MQ=255)

                                                                                  gAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCAt    <  2:2186875/90‑1 (MQ=255)

                                                                                  gAGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCAt    <  1:1376840/90‑1 (MQ=255)

                                                                                   aGCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCAtt   <  1:926350/90‑1 (MQ=255)

                                                                                    gCCGGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTc  <  2:231162/90‑1 (MQ=255)

GTTTGAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTC  >  AC_000091/1045099‑1045272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`