BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,065,700	C→T	R415C (CGT→TGT)	yccE →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,065,700	0	C	T	100.0%	604.7 / NA	144	R415C (CGT→TGT)	yccE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (76/68); total (76/68)

Only 100 of 144 total aligned reads displayed.

TATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGTAT  >  AC_000091/1065613‑1065786

tATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGt                                                                                      <  1:1876477/90‑1 (MQ=255)

 aTGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGtt                                                                                     >  2:906201/1‑90 (MQ=255)

 aTGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGtt                                                                                     <  1:974218/90‑1 (MQ=255)

 aTGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGtt                                                                                     >  1:2424149/1‑90 (MQ=255)

   gACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTAt                                                                                   <  2:1147120/90‑1 (MQ=255)

    aCGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATg                                                                                  <  2:3233074/90‑1 (MQ=255)

        ttACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAAt                                                                              <  1:216940/90‑1 (MQ=255)

        ttACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAAt                                                                              >  2:483747/1‑90 (MQ=255)

        ttACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAAt                                                                              <  2:1892443/90‑1 (MQ=255)

        ttACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAAt                                                                              <  2:1528522/90‑1 (MQ=255)

         tACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAAtt                                                                             <  2:1420246/90‑1 (MQ=255)

          aCCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAAttt                                                                            >  1:1716285/1‑90 (MQ=255)

          aCCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAAttt                                                                            <  2:1960771/90‑1 (MQ=255)

          aCCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAAttt                                                                            <  1:3004943/90‑1 (MQ=255)

           cctccctcCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTa                                                                           >  1:515842/1‑90 (MQ=255)

           cctccctcCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAAGTTa                                                                           >  2:3010879/1‑90 (MQ=255)

              ccctccTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATg                                                                        <  1:2904745/90‑1 (MQ=255)

               cctcctGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATgg                                                                       >  1:3482123/1‑90 (MQ=255)

                  cctGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCaa                                                                    >  2:2198132/1‑90 (MQ=255)

                   ctGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCaaa                                                                   >  1:2110689/1‑90 (MQ=255)

                   ctGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCaaa                                                                   >  2:804733/1‑90 (MQ=255)

                    tGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAg                                                                  <  1:3144584/90‑1 (MQ=255)

                     gAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAgg                                                                 <  2:754512/90‑1 (MQ=255)

                     gAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAgg                                                                 <  1:2941924/90‑1 (MQ=255)

                       aCTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTACTGGCAAAGGCa                                                               <  1:3199690/90‑1 (MQ=255)

                        cTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCat                                                              <  1:1069718/90‑1 (MQ=255)

                          ttttGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCatat                                                            <  2:221538/90‑1 (MQ=255)

                           tttGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATg                                                           >  1:507906/1‑90 (MQ=255)

                              gCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTa                                                        >  1:3462201/1‑90 (MQ=255)

                                tCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTaaa                                                      >  1:2868318/1‑90 (MQ=255)

                                tCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTaaa                                                      >  2:861963/1‑90 (MQ=255)

                                tCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTaaa                                                      <  1:2028071/90‑1 (MQ=255)

                                 cAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTaaaa                                                     >  2:2936035/1‑90 (MQ=255)

                                  aGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTaaaaa                                                    <  2:951828/90‑1 (MQ=255)

                                        aCAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTg                                              >  2:1497460/1‑90 (MQ=255)

                                          aaGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAACCCATTGtt                                            <  1:757109/90‑1 (MQ=255)

                                          aaGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGtt                                            <  2:106015/90‑1 (MQ=255)

                                          aaGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGtt                                            >  1:1494048/1‑90 (MQ=255)

                                           aGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGtta                                           >  1:2135966/1‑90 (MQ=255)

                                            gTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGttat                                          <  2:1662951/90‑1 (MQ=255)

                                             ttAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGttatt                                         <  2:3357542/90‑1 (MQ=255)

                                               aaaCAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAg                                       >  2:311838/1‑90 (MQ=255)

                                                 aCAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGtc                                     <  2:34272/90‑1 (MQ=255)

                                                 aCAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGtc                                     >  1:3302012/1‑90 (MQ=255)

                                                 aCAATAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGtc                                     >  2:2038246/1‑90 (MQ=255)

                                                   aaTAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGtctc                                   >  2:2764319/1‑90 (MQ=255)

                                                    aTAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTca                                  <  1:1198536/90‑1 (MQ=255)

                                                    aTAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTca                                  <  2:1076882/90‑1 (MQ=255)

                                                    aTAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTca                                  >  1:786555/1‑90 (MQ=255)

                                                     tAAGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTcac                                 >  2:1595844/1‑90 (MQ=255)

                                                      aaGGAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTcaca                                >  1:452462/1‑90 (MQ=255)

                                                        ggAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACt                              <  1:674764/90‑1 (MQ=255)

                                                        ggAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACt                              <  2:1922627/90‑1 (MQ=255)

                                                         gAAATGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACtt                             >  2:838845/1‑90 (MQ=255)

                                                          aaaTGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACttt                            <  2:955544/90‑1 (MQ=255)

                                                          aaaTGAATGCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACttt                            >  2:1205121/1‑90 (MQ=255)

                                                           aatgaatgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACtttt                           <  1:2667850/90‑1 (MQ=255)

                                                            atgaatgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACttttt                          <  2:1531631/90‑1 (MQ=255)

                                                             tgaatgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACtttttt                         >  1:546809/1‑90 (MQ=255)

                                                             tgaatgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACtttttt                         >  1:2808152/1‑90 (MQ=255)

                                                             tgaatgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACtttttt                         <  1:3129926/90‑1 (MQ=255)

                                                             tgaatgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACtttttt                         <  1:3263365/90‑1 (MQ=255)

                                                              gaatgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTa                        >  1:2449178/1‑90 (MQ=255)

                                                                atgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTAtt                      >  1:1163735/1‑90 (MQ=255)

                                                                atgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTAtt                      <  1:1221943/90‑1 (MQ=255)

                                                                atgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTAtt                      <  1:2820852/90‑1 (MQ=255)

                                                                atgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTAtt                      <  2:1912834/90‑1 (MQ=255)

                                                                 tgCGCAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTg                     <  1:1372700/90‑1 (MQ=255)

                                                                  gcgcAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTgg                    >  1:188691/1‑90 (MQ=255)

                                                                   cgcAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGt                   >  2:2079367/1‑90 (MQ=255)

                                                                    gcAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTa                  >  1:1517933/1‑90 (MQ=255)

                                                                     cAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTaa                 >  2:402538/1‑90 (MQ=255)

                                                                     cAACTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTaa                 >  2:2669185/1‑90 (MQ=255)

                                                                      aaCTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTaaa                <  1:430916/90‑1 (MQ=255)

                                                                      aaCTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTaaa                >  1:1647618/1‑90 (MQ=255)

                                                                       aCTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAat               >  2:1973711/1‑90 (MQ=255)

                                                                       aCTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAat               >  1:1584454/1‑90 (MQ=255)

                                                                        cTCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAata              <  2:2779906/90‑1 (MQ=255)

                                                                         tCGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAatat             <  1:2409062/90‑1 (MQ=255)

                                                                          cGACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACCTTTTTATTGGTAAATAtt            >  2:910295/1‑90 (MQ=255)

                                                                           gACTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTg           <  1:1487644/90‑1 (MQ=255)

                                                                            aCTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGt          >  2:2071259/1‑90 (MQ=255)

                                                                            aCTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGt          <  1:2216174/90‑1 (MQ=255)

                                                                            aCTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGt          <  2:2445121/90‑1 (MQ=255)

                                                                            aCTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGt          >  1:3185257/1‑90 (MQ=255)

                                                                             cTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGtc         >  2:614276/1‑90 (MQ=255)

                                                                             cTATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGtc         <  2:2439180/90‑1 (MQ=255)

                                                                              tATTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGtct        <  2:1073767/90‑1 (MQ=255)

                                                                               aTTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGtgtc       >  2:1875227/1‑90 (MQ=255)

                                                                               aTTTAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGtctc       <  1:1741027/90‑1 (MQ=255)

                                                                                  tAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGt    <  2:3444174/90‑1 (MQ=255)

                                                                                  tAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGt    >  1:1229125/1‑90 (MQ=255)

                                                                                  tAAATTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGt    <  1:997582/90‑1 (MQ=255)

                                                                                   aaaTTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGTa   >  2:1407083/1‑90 (MQ=255)

                                                                                   aaaTTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGTa   <  1:1383504/90‑1 (MQ=255)

                                                                                   aaaTTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGTa   >  2:327588/1‑90 (MQ=255)

                                                                                    aaTTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAAGCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGTAt  >  2:3439502/1‑90 (MQ=255)

                                                                                    aaTTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGTAt  >  2:2406053/1‑90 (MQ=255)

                                                                                    aaTTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGTAt  <  1:69822/90‑1 (MQ=255)

                                                                                    aaTTGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGTAt  >  2:2489335/1‑90 (MQ=255)

TATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTATTGGTAAATATTGTCTCTGTAT  >  AC_000091/1065613‑1065786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`