BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	49,463	C→T	T565T (ACC→ACT)	kefC →	potassium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	49,463	0	C	T	100.0%	488.9 / NA	120	T565T (ACC→ACT)	kefC	potassium:proton antiporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (49/71); total (49/71)

Only 100 of 120 total aligned reads displayed.

GCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCATTAATTCA  >  AC_000091/49376‑49549

gCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTaa                                                                                      >  1:2002660/1‑90 (MQ=255)

gCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTaa                                                                                      <  1:1962403/90‑1 (MQ=255)

 ccGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTaaa                                                                                     <  2:948189/90‑1 (MQ=255)

 ccGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTaaa                                                                                     <  1:1885407/90‑1 (MQ=255)

  cGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAg                                                                                    <  1:3179017/90‑1 (MQ=255)

  cGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAg                                                                                    >  2:3407936/1‑90 (MQ=255)

  cGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAg                                                                                    <  2:2828617/90‑1 (MQ=255)

   gTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGc                                                                                   >  1:1117951/1‑90 (MQ=255)

      tGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCcg                                                                                >  1:2148545/1‑90 (MQ=255)

      tGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCcg                                                                                <  2:2631805/90‑1 (MQ=255)

        aaGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCcgcg                                                                              <  2:3466082/90‑1 (MQ=255)

         aGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCcgcgc                                                                             >  2:1059749/1‑90 (MQ=255)

         aGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCcgcgc                                                                             <  2:1812345/90‑1 (MQ=255)

           cgcgAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCgcgg                                                                           >  2:1839811/1‑90 (MQ=255)

            gcgAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCgcggc                                                                          >  1:1049819/1‑90 (MQ=255)

             cgAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCgcggcg                                                                         <  1:288294/90‑1 (MQ=255)

              gagaACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGcggcgg                                                                        >  1:3091188/1‑90 (MQ=255)

              gagaACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGcggcgg                                                                        >  2:2599404/1‑90 (MQ=255)

              gagaACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGcggcgg                                                                        >  1:1422359/1‑90 (MQ=255)

              gagaACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGcggcgg                                                                        >  2:60209/1‑90 (MQ=255)

                gaACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTc                                                                      <  1:1666860/90‑1 (MQ=255)

                 aaCGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCt                                                                     <  2:1932672/90‑1 (MQ=255)

                 aaCGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCt                                                                     <  1:3296885/90‑1 (MQ=255)

                   cGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCtat                                                                   <  2:1842118/90‑1 (MQ=255)

                     tGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATaa                                                                 <  1:67753/90‑1 (MQ=255)

                       ccGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAAc                                                               >  1:2010388/1‑90 (MQ=255)

                       ccGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAAc                                                               <  1:3461979/90‑1 (MQ=255)

                         gATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGc                                                             >  2:3106720/1‑90 (MQ=255)

                          aTGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCa                                                            >  2:1417246/1‑90 (MQ=255)

                           tgtgTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCAc                                                           >  2:1919682/1‑90 (MQ=255)

                           tgtgTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCAc                                                           <  1:3005706/90‑1 (MQ=255)

                            gtgtTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCAcc                                                          >  1:1581029/1‑90 (MQ=255)

                               ttCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAgc                                                       >  2:3299228/1‑90 (MQ=255)

                               ttCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAgc                                                       <  2:832283/90‑1 (MQ=255)

                               ttCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAgc                                                       >  1:3437774/1‑90 (MQ=255)

                               ttCAGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAgc                                                       <  2:350155/90‑1 (MQ=255)

                                tCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAgcg                                                      >  1:1286110/1‑90 (MQ=255)

                                  cgccgcTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGcgcg                                                    <  2:2424684/90‑1 (MQ=255)

                                  cgccgcTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGcgcg                                                    >  1:3279281/1‑90 (MQ=255)

                                   gccgcTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGa                                                   <  1:1795901/90‑1 (MQ=255)

                                    ccgcTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGAt                                                  <  1:1778966/90‑1 (MQ=255)

                                    ccgcTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGAt                                                  <  1:3272968/90‑1 (MQ=255)

                                     cgcTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATg                                                 <  1:1159890/90‑1 (MQ=255)

                                     agcTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATg                                                 >  2:3245703/2‑90 (MQ=255)

                                      gcTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGt                                                >  2:877698/1‑90 (MQ=255)

                                       cTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGtt                                               <  1:1558059/90‑1 (MQ=255)

                                       cTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGtt                                               >  1:2960950/1‑90 (MQ=255)

                                       cTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGtt                                               <  1:1269552/90‑1 (MQ=255)

                                       cTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGtt                                               <  2:2157916/90‑1 (MQ=255)

                                          tAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAg                                            >  2:249223/1‑90 (MQ=255)

                                          tAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAg                                            <  2:1100436/90‑1 (MQ=255)

                                           aaTATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGt                                           >  2:1362230/1‑90 (MQ=255)

                                             tatTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTga                                         <  1:2711503/90‑1 (MQ=255)

                                             tatTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTga                                         <  2:644215/90‑1 (MQ=255)

                                               ttCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTgaga                                       >  2:2678171/1‑90 (MQ=255)

                                                tCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGAt                                      <  1:513472/90‑1 (MQ=255)

                                                tCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGAt                                      <  1:2023504/90‑1 (MQ=255)

                                                 cAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATc                                     <  2:523865/90‑1 (MQ=255)

                                                 cAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATc                                     <  1:2846173/90‑1 (MQ=255)

                                                  aGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCa                                    >  1:1100869/1‑90 (MQ=255)

                                                  aGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCa                                    >  1:1085343/1‑90 (MQ=255)

                                                   gATGGTGGAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCAt                                   >  1:2692731/1‑90 (MQ=255)

                                                     tggtggAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTa                                 <  1:1300837/90‑1 (MQ=255)

                                                      ggtggAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTAc                                >  1:574756/1‑90 (MQ=255)

                                                      ggtggAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTAc                                <  2:3431819/90‑1 (MQ=255)

                                                       gtggAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTAcc                               <  2:3438463/90‑1 (MQ=255)

                                                       gtggAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTAcc                               <  1:3116628/90‑1 (MQ=255)

                                                       gtggAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTAcc                               <  1:1602428/90‑1 (MQ=255)

                                                        tggAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCg                              <  1:2783292/90‑1 (MQ=255)

                                                        tggAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCg                              >  1:2662331/1‑90 (MQ=255)

                                                         ggAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGa                             <  1:2634380/90‑1 (MQ=255)

                                                         ggAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGa                             <  1:3236540/90‑1 (MQ=255)

                                                          gAAGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAg                            >  2:1658257/1‑90 (MQ=255)

                                                           aaGAGATGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAgg                           <  1:1863754/90‑1 (MQ=255)

                                                              agaTGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGAcc                        >  1:981074/1‑90 (MQ=255)

                                                               gaTGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACcg                       >  1:251832/1‑90 (MQ=255)

                                                                aTGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACcgc                      <  2:1996326/90‑1 (MQ=255)

                                                                 tGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACcgcg                     <  2:794521/90‑1 (MQ=255)

                                                                 tGGCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACcgcg                     <  1:3355376/90‑1 (MQ=255)

                                                                   gCAATGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGaa                   <  1:2815995/90‑1 (MQ=255)

                                                                      aTGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACAt                <  2:2041703/90‑1 (MQ=255)

                                                                      aTGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACAt                >  1:2428243/1‑90 (MQ=255)

                                                                      aTGGTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACAt                <  1:2315663/90‑1 (MQ=255)

                                                                        ggTTGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCt              <  2:301297/90‑1 (MQ=255)

                                                                          ttGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGt            <  1:2223631/90‑1 (MQ=255)

                                                                          ttGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGt            <  1:2040313/90‑1 (MQ=255)

                                                                          ttGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGt            >  2:2679750/1‑90 (MQ=255)

                                                                           tGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTc           <  2:321728/90‑1 (MQ=255)

                                                                           tGAGAACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTc           <  1:1484914/90‑1 (MQ=255)

                                                                              gaACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCAtt        >  2:2084084/1‑90 (MQ=255)

                                                                              gaACGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCAtt        <  1:1314442/90‑1 (MQ=255)

                                                                               aaCGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCATTa       >  2:490687/1‑90 (MQ=255)

                                                                               aaCGACACTAAAGCCCGCGCGGCGGGCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCATTa       <  2:2312631/90‑1 (MQ=255)

                                                                                aCGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGTCCGCGAACATCTGTCATTaa      <  2:2431702/90‑1 (MQ=255)

                                                                                aCGACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCATTaa      <  1:157042/90‑1 (MQ=255)

                                                                                  gACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCATTAAtt    >  1:2627688/1‑90 (MQ=255)

                                                                                  gACACTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCATTAAtt    <  2:2350343/90‑1 (MQ=255)

                                                                                   acacTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCATTAATTc   <  2:434886/90‑1 (MQ=255)

                                                                                    cacTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCATTAATTCa  <  1:2593598/90‑1 (MQ=255)

                                                                                    cacTAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCATTAATTCa  <  2:73988/90‑1 (MQ=255)

GCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCATTAATTCA  >  AC_000091/49376‑49549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`