BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,091,749	C→T	S321S (TCG→TCA)	ycdS ←	predicted outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,091,749	0	C	T	100.0%	537.8 / NA	134	S321S (TCG→TCA)	ycdS	predicted outer membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (63/71); total (63/71)

Only 100 of 136 total aligned reads displayed.

TAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCCGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACG  >  AC_000091/1091662‑1091835

tAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGa                                                                                      <  1:1740043/90‑1 (MQ=255)

 aaaTCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGAt                                                                                     <  2:1826108/90‑1 (MQ=255)

    tCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCa                                                                                  >  1:2135305/1‑90 (MQ=255)

       ggggCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAAcc                                                                               >  1:3157442/1‑90 (MQ=255)

          gCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCaa                                                                            >  2:1590089/1‑90 (MQ=255)

            aaTGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAata                                                                          >  2:3276045/1‑90 (MQ=255)

            aaTGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAata                                                                          >  1:2018242/1‑90 (MQ=255)

            aaTGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAata                                                                          <  1:1049403/90‑1 (MQ=255)

             aTGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAatat                                                                         >  2:2296197/1‑90 (MQ=255)

              tGGTCTTCTTGTGATAAAAGAGCTCGGTTATTATTGACTGTGCTTTTTTCGGGTGATGATCTTTGTGATAAGCTGATGCAACCCAATAtt                                                                        <  2:3411792/90‑1 (MQ=255)

              tGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATAtt                                                                        <  2:3026476/90‑1 (MQ=255)

              tGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATAtt                                                                        <  1:902518/90‑1 (MQ=255)

                gTCTCCTTGTGATAAACGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGc                                                                      <  1:2530069/90‑1 (MQ=255)

                 tctcCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGcc                                                                     >  1:2155981/1‑90 (MQ=255)

                  ctcCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGccc                                                                    <  2:1416139/90‑1 (MQ=255)

                   tcCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGcccc                                                                   >  2:3366661/1‑90 (MQ=255)

                     cTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCa                                                                 <  1:2691799/90‑1 (MQ=255)

                      ttGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAg                                                                <  2:3360979/90‑1 (MQ=255)

                      ttGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAg                                                                >  2:1699457/1‑90 (MQ=255)

                       tgtgATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAgg                                                               >  2:78488/1‑90 (MQ=255)

                       tgtgATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAgg                                                               <  1:1276860/90‑1 (MQ=255)

                          gATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCg                                                            <  1:2682568/90‑1 (MQ=255)

                           aTAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCgg                                                           >  2:1696516/1‑90 (MQ=255)

                            tAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCNNNTGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGa                                                          <  2:1044254/90‑1 (MQ=255)

                            tAAAAAGGCCCGGTCATTA‑TGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGaa                                                         <  2:3385911/90‑1 (MQ=255)

                             aaaaGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGaa                                                         >  1:1168241/1‑90 (MQ=255)

                              aaaGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGaat                                                        >  1:2371827/1‑90 (MQ=255)

                               aaGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGaata                                                       <  2:1270476/90‑1 (MQ=255)

                               aaGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGaata                                                       >  1:721152/1‑90 (MQ=255)

                                agagCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGaataa                                                      >  1:1046036/1‑90 (MQ=255)

                                 gagCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGaataat                                                     >  1:1323422/1‑90 (MQ=255)

                                 gagCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGaataat                                                     >  1:2810865/1‑90 (MQ=255)

                                  agCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAAtt                                                    <  2:2361754/90‑1 (MQ=255)

                                   gCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAAttt                                                   >  2:1362517/1‑90 (MQ=255)

                                   gCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAAttt                                                   >  2:2060786/1‑90 (MQ=255)

                                    cTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTg                                                  >  1:2019358/1‑90 (MQ=255)

                                      cGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGcc                                                >  2:639421/1‑90 (MQ=255)

                                       ggTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGccc                                               >  2:2512276/1‑90 (MQ=255)

                                         tCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTTCCCCCAGGGCGGAATAATTTGCCCCg                                             <  1:3265236/90‑1 (MQ=255)

                                           attattGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTc                                           >  2:2637403/1‑90 (MQ=255)

                                            ttattGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCt                                          >  2:3181555/1‑90 (MQ=255)

                                             tattGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCtt                                         <  2:212185/90‑1 (MQ=255)

                                               ttGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCtttt                                       >  2:3415295/1‑90 (MQ=255)

                                               ttGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCtttt                                       <  1:1610047/90‑1 (MQ=255)

                                                tGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCttttt                                      <  1:399731/90‑1 (MQ=255)

                                                 gACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCtttttt                                     >  1:1428042/1‑90 (MQ=255)

                                                  aCTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTa                                    >  1:3319822/1‑90 (MQ=255)

                                                  aCTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTa                                    >  1:490500/1‑90 (MQ=255)

                                                   cTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTaa                                   >  1:1202256/1‑90 (MQ=255)

                                                   cTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTaa                                   >  1:2015733/1‑90 (MQ=255)

                                                    tgtgCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAAt                                  <  1:3012080/90‑1 (MQ=255)

                                                    tgtgCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAAt                                  <  2:1531936/90‑1 (MQ=255)

                                                     gtgCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATc                                 <  1:1255310/90‑1 (MQ=255)

                                                      tgCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATct                                >  2:1278130/1‑89 (MQ=255)

                                                      tgCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCg                                <  2:1237871/90‑1 (MQ=255)

                                                       gCTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGc                               <  1:3281081/90‑1 (MQ=255)

                                                        cTTTTTTCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCt                              >  1:3373883/1‑90 (MQ=255)

                                                         ttttttCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTg                             >  2:574868/1‑90 (MQ=255)

                                                         ttttttCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTg                             <  2:2737932/90‑1 (MQ=255)

                                                         ttttttCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTg                             >  1:2559305/1‑90 (MQ=255)

                                                             ttCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAg                         <  1:934226/90‑1 (MQ=255)

                                                             ttCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAg                         <  2:3415389/90‑1 (MQ=255)

                                                              tCGGCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGt                        <  2:2928272/90‑1 (MQ=255)

                                                                ggCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTga                      <  2:2514215/90‑1 (MQ=255)

                                                                 gCTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTgag                     <  2:1776311/90‑1 (MQ=255)

                                                                  cTGATGATCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTgaga                    >  2:1983794/1‑90 (MQ=255)

                                                                   tgatgaTCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGaa                   >  2:3175578/1‑90 (MQ=255)

                                                                   tgatgaTCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGaa                   >  1:2491806/1‑90 (MQ=255)

                                                                    gatgatCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGaaa                  <  1:3366661/90‑1 (MQ=255)

                                                                     atgatCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAAt                 <  1:2258064/90‑1 (MQ=255)

                                                                      tgatCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATa                <  2:2300804/90‑1 (MQ=255)

                                                                       gatCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATaa               >  2:2730856/1‑90 (MQ=255)

                                                                        atCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAAc              <  2:2821037/90‑1 (MQ=255)

                                                                        atCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAAc              <  1:2338003/90‑1 (MQ=255)

                                                                        atCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAAc              >  2:3067741/1‑90 (MQ=255)

                                                                        atCTTTGAGATAAGCTCATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAAc              <  1:850957/90‑1 (MQ=255)

                                                                         tCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACg             <  2:2156168/90‑1 (MQ=255)

                                                                         tCTTTGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACg             <  1:2934319/90‑1 (MQ=255)

                                                                           tttGAGATGAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTc           >  2:2851077/1‑90 (MQ=255)

                                                                           tttGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTc           <  2:1719248/90‑1 (MQ=255)

                                                                           tttGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTc           >  1:1111808/1‑90 (MQ=255)

                                                                           tttGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTc           <  1:183879/90‑1 (MQ=255)

                                                                           tttGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTc           <  2:3300112/90‑1 (MQ=255)

                                                                           tttGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTc           <  1:2695774/90‑1 (MQ=255)

                                                                            ttGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCt          >  1:155742/1‑90 (MQ=255)

                                                                            ttGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCt          >  1:2849045/1‑90 (MQ=255)

                                                                            ttGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCt          >  2:2115859/1‑90 (MQ=255)

                                                                             tGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCtt         >  2:1992355/1‑90 (MQ=255)

                                                                             tGAGATAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCtt         >  2:2356083/1‑90 (MQ=255)

                                                                                gaTAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTtat      <  2:1318823/90‑1 (MQ=255)

                                                                                 aTAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCCTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTtata     <  2:154447/90‑1 (MQ=255)

                                                                                  tAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATaa    <  1:1836295/90‑1 (MQ=255)

                                                                                  tAAGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATaa    <  1:1440506/90‑1 (MQ=255)

                                                                                   aaGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAAc   <  2:749611/90‑1 (MQ=255)

                                                                                   aaGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAAc   <  1:1286625/90‑1 (MQ=255)

                                                                                   aaGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAAc   <  1:799047/90‑1 (MQ=255)

                                                                                   aaGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAAc   >  1:2838952/1‑90 (MQ=255)

                                                                                    aGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACg  <  2:548716/90‑1 (MQ=255)

                                                                                    aGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACg  <  1:1395680/90‑1 (MQ=255)

                                                                                    aGCTGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACg  >  2:1864604/1‑90 (MQ=255)

TAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGGCTGATGATCTTTGAGATAAGCCGATGCAACCCAATATTGCCCCCAGGGCGGAATAATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACG  >  AC_000091/1091662‑1091835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`