BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,093,686	T→C	V130A (GTA→GCA)	ycdT →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,093,686	0	T	C	100.0%	499.6 / NA	126	V130A (GTA→GCA)	ycdT	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (60/66); total (60/66)

Only 100 of 126 total aligned reads displayed.

CGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTT  >  AC_000091/1093601‑1093772

cGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTg                                                                                    >  1:3211213/1‑90 (MQ=255)

cGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTg                                                                                    >  1:2487026/1‑90 (MQ=255)

   ttGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTg                                                                                 <  2:718818/90‑1 (MQ=255)

   ttGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTg                                                                                 >  2:485647/1‑90 (MQ=255)

       cAAGCAGTGTTGTCCAAAATTACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATa                                                                             <  2:326959/90‑1 (MQ=255)

       cAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATa                                                                             >  2:515006/1‑90 (MQ=255)

        aaGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATaa                                                                            >  1:645819/1‑90 (MQ=255)

        aaGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATaa                                                                            >  2:2200094/1‑90 (MQ=255)

        aaGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATaa                                                                            >  1:2143188/1‑90 (MQ=255)

         aGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATaaa                                                                           <  1:1079380/90‑1 (MQ=255)

          gCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAg                                                                          <  2:2118059/90‑1 (MQ=255)

           cAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGGGAATAAAGt                                                                         >  1:580003/1‑90 (MQ=255)

            aGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                                                        >  2:1537499/1‑90 (MQ=255)

            aGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                                                        >  2:798035/1‑90 (MQ=255)

             gtgtTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTa                                                                       <  2:3208460/90‑1 (MQ=255)

             gtgtTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTa                                                                       <  2:2359399/90‑1 (MQ=255)

              tgttgtCCAAAATGCCATTGCAATCTATTATTTGTTTAGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg                                                                      >  2:1549064/1‑90 (MQ=255)

              tgttgtCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGCGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg                                                                      <  2:3222336/90‑1 (MQ=255)

               gttgtCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGt                                                                     >  2:207514/1‑90 (MQ=255)

                 tgtCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa                                                                   <  1:1289245/90‑1 (MQ=255)

                  gtCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                                                  <  2:1111852/90‑1 (MQ=255)

                     cAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAac                                                               >  2:1738750/1‑90 (MQ=255)

                       aaaTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAacac                                                             <  2:1588061/90‑1 (MQ=255)

                       aaaTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAacac                                                             <  1:603451/90‑1 (MQ=255)

                        aaTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAAcaca                                                            >  1:1089628/1‑90 (MQ=255)

                        aaTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAAcaca                                                            <  1:3164916/90‑1 (MQ=255)

                         aTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                                                           <  1:1562356/90‑1 (MQ=255)

                         aTGACAGTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAAcacag                                                           >  2:2290306/1‑89 (MQ=255)

                          tGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaa                                                          >  2:1942448/1‑90 (MQ=255)

                           gACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa                                                         >  2:727630/1‑90 (MQ=255)

                           gACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa                                                         >  2:3410910/1‑90 (MQ=255)

                            aCATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAAc                                                        <  1:203993/90‑1 (MQ=255)

                             cATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACa                                                       <  1:2650512/90‑1 (MQ=255)

                             cATTGCAATCTATGATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGCAAACACAAAACa                                                       <  2:2287565/90‑1 (MQ=255)

                              aTTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAg                                                      <  1:2574792/90‑1 (MQ=255)

                               ttGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                                                     <  2:1648504/90‑1 (MQ=255)

                               ttGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                                                     <  2:916171/90‑1 (MQ=255)

                               ttGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                                                     <  1:1218675/90‑1 (MQ=255)

                               ttGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                                                     <  1:2874678/90‑1 (MQ=255)

                                tGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgcg                                                    >  1:2327066/1‑90 (MQ=255)

                                 gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgcgc                                                   <  2:27617/90‑1 (MQ=255)

                                  cAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCa                                                  <  2:2517917/90‑1 (MQ=255)

                                    aTCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAat                                                >  1:757632/1‑90 (MQ=255)

                                     tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatt                                               <  2:1194571/90‑1 (MQ=255)

                                      cTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt                                              >  1:1814266/1‑90 (MQ=255)

                                       tattatTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta                                             >  2:2689425/1‑90 (MQ=255)

                                       tattatTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta                                             >  1:187138/1‑90 (MQ=255)

                                       tattatTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta                                             <  1:1586109/90‑1 (MQ=255)

                                        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttat                                            >  1:2134532/1‑90 (MQ=255)

                                          tattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttattt                                          <  2:1925667/90‑1 (MQ=255)

                                          tattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttattt                                          <  2:1821176/90‑1 (MQ=255)

                                          tattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttattt                                          >  1:701330/1‑90 (MQ=255)

                                           attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTAtttt                                         >  2:3368316/1‑90 (MQ=255)

                                            tttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTc                                        <  2:2919192/90‑1 (MQ=255)

                                             ttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTcc                                       <  2:3294010/90‑1 (MQ=255)

                                              tGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCa                                      >  2:2065098/1‑90 (MQ=255)

                                               gTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaa                                     >  1:3458747/1‑90 (MQ=255)

                                                 ttCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaa                                   >  2:1585461/1‑90 (MQ=255)

                                                   cGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa                                 >  1:1300153/1‑90 (MQ=255)

                                                    gTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaaa                                <  1:2588968/90‑1 (MQ=255)

                                                     tCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt                               <  2:2344133/90‑1 (MQ=255)

                                                     tCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt                               <  1:287531/90‑1 (MQ=255)

                                                     tCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt                               <  1:1237539/90‑1 (MQ=255)

                                                     tCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt                               >  1:1856730/1‑90 (MQ=255)

                                                      cAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg                              <  1:53260/90‑1 (MQ=255)

                                                        gATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAGACACAAAACAGCGCAATATATTTTCCAAAAAAATGAc                            >  1:1705383/1‑90 (MQ=255)

                                                         aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACt                           <  2:682443/90‑1 (MQ=255)

                                                          tGAGTTTTTGCATATTTATATTTCTGGCATTGGTGAATAAAGTTTGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACtt                          >  2:1104380/1‑90 (MQ=255)

                                                             gTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtgt                       >  1:82177/1‑90 (MQ=255)

                                                             gTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtgt                       >  1:3198717/1‑90 (MQ=255)

                                                                tgtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCa                    >  2:2521903/1‑90 (MQ=255)

                                                                 gtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCAt                   <  2:718170/90‑1 (MQ=255)

                                                                  tgCATATTAATATCTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATa                  <  2:333140/90‑1 (MQ=255)

                                                                   gCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATaa                 >  2:814674/1‑90 (MQ=255)

                                                                    cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAg                <  2:1501726/90‑1 (MQ=255)

                                                                    cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAg                >  1:3000588/1‑90 (MQ=255)

                                                                     atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAgt               <  2:2462309/90‑1 (MQ=255)

                                                                     atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAgt               <  1:887308/90‑1 (MQ=255)

                                                                      tatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAgtt              <  2:3479261/90‑1 (MQ=255)

                                                                      tatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAgtt              <  1:413378/90‑1 (MQ=255)

                                                                       atTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAgttt             >  1:3409859/1‑90 (MQ=255)

                                                                       atTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAgttt             <  1:1738427/90‑1 (MQ=255)

                                                                        ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAgtttg            <  2:2101928/90‑1 (MQ=255)

                                                                         tAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAgtttgt           >  1:2615560/1‑90 (MQ=255)

                                                                         tAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAgtttgt           >  2:1046796/1‑90 (MQ=255)

                                                                          aaTATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAgtttgtt          >  1:801530/1‑90 (MQ=255)

                                                                           atatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAgtttgttt         <  2:3156231/90‑1 (MQ=255)

                                                                            tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgtttt        >  1:549972/1‑90 (MQ=255)

                                                                            tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgtttt        >  2:1124616/1‑90 (MQ=255)

                                                                            tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgtttt        >  1:1859828/1‑90 (MQ=255)

                                                                            tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgtttt        <  2:1659798/90‑1 (MQ=255)

                                                                             atTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgttttt       <  1:383321/90‑1 (MQ=255)

                                                                              tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgtttttt      <  2:1396154/90‑1 (MQ=255)

                                                                              tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgtttttt      <  1:1433755/90‑1 (MQ=255)

                                                                               ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAANANNNCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgttttttn     <  2:2359675/90‑2 (MQ=255)

                                                                                  tGGCATTGGTGAATAAAGTTAGTGAACACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgttttttgttt  <  1:1611922/90‑1 (MQ=255)

                                                                                  tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgttttttgttt  <  2:1242205/90‑1 (MQ=255)

                                                                                  tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgttttttgttt  <  1:498348/90‑1 (MQ=255)

                                                                                  tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgttttttgttt  >  2:1666419/1‑90 (MQ=255)

                                                                                  tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGtttgttttttgttt  >  2:1823478/1‑90 (MQ=255)

CGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTT  >  AC_000091/1093601‑1093772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`