BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,194,589	A→G	G254G (GGT→GGC)	trmU ←	tRNA (5‑methylaminomethyl‑2‑thiouridylate)‑ methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,194,589	0	A	G	100.0%	409.7 / NA	113	G254G (GGT→GGC)	trmU	tRNA (5‑methylaminomethyl‑2‑thiouridylate)‑ methyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (76/37); total (76/37)

Only 100 of 114 total aligned reads displayed.

CATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCC  >  AC_000091/1194502‑1194675

caTCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGcc                                                                                      >  1:2838664/1‑90 (MQ=255)

 aTCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCct                                                                                     <  2:785063/90‑1 (MQ=255)

  tCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCctt                                                                                    >  1:3290419/1‑90 (MQ=255)

  tCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCctt                                                                                    <  1:3065735/90‑1 (MQ=255)

      ccGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCttt                                                                                <  2:2711316/90‑1 (MQ=255)

       cgcgGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTg                                                                               <  2:1798755/90‑1 (MQ=255)

        gcgGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTgg                                                                              >  2:3320664/1‑90 (MQ=255)

        gcgGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTgg                                                                              <  2:1371839/90‑1 (MQ=255)

           ggTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGc                                                                           >  2:740874/1‑90 (MQ=255)

           ggTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGc                                                                           >  1:2514610/1‑90 (MQ=255)

             tgtTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCa                                                                         >  1:1560424/1‑90 (MQ=255)

             tgtTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCa                                                                         >  2:2145510/1‑90 (MQ=255)

               ttCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCAcc                                                                       >  1:1915892/1‑90 (MQ=255)

               ttCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCAcc                                                                       <  2:731065/90‑1 (MQ=255)

                tCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCg                                                                      >  2:3044477/1‑90 (MQ=255)

                tCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCg                                                                      >  1:2518069/1‑90 (MQ=255)

                 cATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGa                                                                     >  2:1654902/1‑90 (MQ=255)

                  aTGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGAt                                                                    >  2:15310/1‑90 (MQ=255)

                   tGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATa                                                                   >  2:335225/1‑90 (MQ=255)

                   tGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATa                                                                   >  1:2057737/1‑90 (MQ=255)

                     gCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATAcc                                                                 <  1:2845702/90‑1 (MQ=255)

                     gCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATAcc                                                                 >  1:735344/1‑90 (MQ=255)

                     gCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATAcc                                                                 >  2:1125597/1‑90 (MQ=255)

                     gCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATAcc                                                                 >  2:344310/1‑90 (MQ=255)

                      cccTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATAccc                                                                >  1:2411659/1‑90 (MQ=255)

                       ccTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCa                                                               >  2:1270322/1‑90 (MQ=255)

                        cTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGGGCCACCGATACCCAg                                                              >  1:873453/1‑90 (MQ=255)

                         tGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGa                                                             >  2:819949/1‑90 (MQ=255)

                          gAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGATGCCACCCATACCCAGAc                                                            <  2:3062952/90‑1 (MQ=255)

                           aGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGAcc                                                           >  2:622469/1‑90 (MQ=255)

                             cGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCtt                                                         >  1:1251672/1‑90 (MQ=255)

                              gACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCttt                                                        >  2:1856962/1‑90 (MQ=255)

                              gACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCttt                                                        >  1:1122923/1‑90 (MQ=255)

                               aCAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTa                                                       >  1:1916182/1‑90 (MQ=255)

                                 aaCCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACg                                                     >  2:1696226/1‑90 (MQ=255)

                                 aaCCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACg                                                     >  2:1764731/1‑90 (MQ=255)

                                   ccAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCt                                                   >  2:657559/1‑90 (MQ=255)

                                    cAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTg                                                  >  1:135078/1‑90 (MQ=255)

                                    cAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTg                                                  >  1:2402785/1‑90 (MQ=255)

                                    cAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTg                                                  <  2:126140/90‑1 (MQ=255)

                                     aGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGa                                                 <  2:3069540/90‑1 (MQ=255)

                                      gAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGAc                                                >  1:1658710/1‑90 (MQ=255)

                                      gAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGAc                                                <  1:2790959/90‑1 (MQ=255)

                                       aaTGTTGTTTTCGACGTCTTTGTCCCCCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGAcc                                               <  2:2213830/90‑1 (MQ=255)

                                        attttgttTTCGACGTCTTTGTCCACCACATACCCCGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCg                                              <  2:1753311/87‑1 (MQ=255)

                                        aTGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGATCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCg                                              <  2:1844885/90‑1 (MQ=255)

                                            tgttTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGagag                                          >  2:2233185/1‑90 (MQ=255)

                                            tgttTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGagag                                          >  2:3230173/1‑90 (MQ=255)

                                             gttTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAgt                                         <  2:1834255/90‑1 (MQ=255)

                                             gttTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAgt                                         >  1:532383/1‑90 (MQ=255)

                                                ttCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGtgtg                                      >  2:1511148/1‑90 (MQ=255)

                                                 tCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGa                                     >  2:2216957/1‑90 (MQ=255)

                                                   gACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATa                                   <  2:1369504/90‑1 (MQ=255)

                                                   gACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATa                                   >  2:216751/1‑90 (MQ=255)

                                                    aCGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATAc                                  <  2:800673/90‑1 (MQ=255)

                                                     cGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACa                                 >  1:2676136/1‑90 (MQ=255)

                                                     cGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACa                                 >  1:191615/1‑90 (MQ=255)

                                                      gTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACAt                                >  1:970498/1‑90 (MQ=255)

                                                      gTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACAt                                <  1:2316478/90‑1 (MQ=255)

                                                       tCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTTGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATc                               <  1:1844987/90‑1 (MQ=255)

                                                       tCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATc                               >  2:2302231/1‑90 (MQ=255)

                                                        cTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCa                              >  1:3226905/1‑90 (MQ=255)

                                                         tttGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAg                             >  1:2035701/1‑90 (MQ=255)

                                                           tGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGcc                           <  1:511764/90‑1 (MQ=255)

                                                            gTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGccc                          <  2:428690/90‑1 (MQ=255)

                                                             tCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGcccc                         >  2:3209698/1‑90 (MQ=255)

                                                                accacATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTgg                      >  1:12644/1‑90 (MQ=255)

                                                                 ccacATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGt                     >  2:2518418/1‑90 (MQ=255)

                                                                 ccacATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGt                     >  1:2234852/1‑90 (MQ=255)

                                                                  cacaTACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTg                    <  2:1863488/90‑1 (MQ=255)

                                                                  cacaTACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTg                    >  2:772317/1‑90 (MQ=255)

                                                                   acaTACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGc                   >  2:2423576/1‑90 (MQ=255)

                                                                      tACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCa                <  2:1202402/90‑1 (MQ=255)

                                                                       aCCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCAc               >  1:397953/1‑90 (MQ=255)

                                                                       aCCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCAc               >  1:1330335/1‑90 (MQ=255)

                                                                       aCCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGAGACATCAGCCCCTGGTGCTCAc               >  2:971565/1‑90 (MQ=255)

                                                                        ccACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCAcc              >  2:2768085/1‑90 (MQ=255)

                                                                         ctcGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCa             <  2:501838/88‑1 (MQ=255)

                                                                         cACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCa             <  1:853309/90‑1 (MQ=255)

                                                                           cGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAAt           >  1:2712191/1‑90 (MQ=255)

                                                                           cGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAAt           >  2:3103316/1‑90 (MQ=255)

                                                                           cGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAAt           <  1:2386081/90‑1 (MQ=255)

                                                                           cGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAAt           <  1:2862566/90‑1 (MQ=255)

                                                                            ggTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAAtt          <  2:2728903/90‑1 (MQ=255)

                                                                            ggTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAAtt          >  1:2714429/1‑90 (MQ=255)

                                                                            ggTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAAtt          >  2:1925859/1‑90 (MQ=255)

                                                                             gTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACNNTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAAttt         <  2:1875239/90‑1 (MQ=255)

                                                                               tcttcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCa       >  2:1269747/1‑90 (MQ=255)

                                                                               tcttcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCa       >  1:857743/1‑90 (MQ=255)

                                                                                cttcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCAt      <  1:911944/90‑1 (MQ=255)

                                                                                cttcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCAt      <  2:2741240/90‑1 (MQ=255)

                                                                                cttcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCAt      >  2:1740641/1‑90 (MQ=255)

                                                                                cttcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCAt      <  2:1745192/90‑1 (MQ=255)

                                                                                cttcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCAt      >  2:2101573/1‑90 (MQ=255)

                                                                                 ttcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATc     >  1:3337969/1‑90 (MQ=255)

                                                                                 ttcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATc     >  2:2244469/1‑90 (MQ=255)

                                                                                  tcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCg    >  2:1769899/1‑90 (MQ=255)

                                                                                   cGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGc   >  2:3054050/1‑90 (MQ=255)

                                                                                   cGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGc   <  1:1025553/90‑1 (MQ=255)

                                                                                    ggTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGcc  <  1:1534311/90‑1 (MQ=255)

CATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCC  >  AC_000091/1194502‑1194675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`