BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,292,378	T→C	V187A (GTG→GCG)	rssB →	response regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,292,378	0	T	C	100.0%	419.8 / NA	108	V187A (GTG→GCG)	rssB	response regulator of RpoS
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (50/58); total (50/58)

Only 100 of 111 total aligned reads displayed.

GGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTG  >  AC_000091/1292292‑1292464

ggAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCt                                                                                     >  1:222143/1‑90 (MQ=255)

  aaCTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTg                                                                                   >  1:1164597/1‑90 (MQ=255)

   aCTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGNNNTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGa                                                                                  <  2:810603/90‑1 (MQ=255)

   aCTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGa                                                                                  <  1:2350431/90‑1 (MQ=255)

    cgacacCCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGat                                                                                 <  1:1270139/88‑1 (MQ=255)

    cTACACCCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGat                                                                                 <  2:2180336/90‑1 (MQ=255)

    cTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGat                                                                                 <  1:1798773/90‑1 (MQ=255)

        caCCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTg                                                                             <  2:518339/89‑1 (MQ=255)

        aaCCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTg                                                                             >  2:245425/1‑90 (MQ=255)

        aaCCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTg                                                                             >  2:1213433/1‑90 (MQ=255)

        aaCCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTg                                                                             <  1:2240172/90‑1 (MQ=255)

         aCCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGc                                                                            <  2:1611191/90‑1 (MQ=255)

          ccgccgGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGcc                                                                           >  2:2951046/1‑90 (MQ=255)

            gccgGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGc                                                                         >  1:492143/1‑90 (MQ=255)

              cgGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCAc                                                                       <  1:1349152/90‑1 (MQ=255)

               ggTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTCTCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACt                                                                      >  2:1530121/1‑90 (MQ=255)

               ggTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACt                                                                      <  1:888850/90‑1 (MQ=255)

               ggTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACt                                                                      <  2:2978749/90‑1 (MQ=255)

               ggTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACt                                                                      <  2:1682722/90‑1 (MQ=255)

                 ttCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACttt                                                                    <  1:1565751/90‑1 (MQ=255)

                   cagcagGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCg                                                                  <  2:420989/90‑1 (MQ=255)

                   cagcagGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCg                                                                  <  1:1685668/90‑1 (MQ=255)

                     gcagGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGa                                                                <  1:1766853/90‑1 (MQ=255)

                     gcagGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGa                                                                <  1:2008723/90‑1 (MQ=255)

                      cagGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGaa                                                               >  2:904338/1‑90 (MQ=255)

                      cagGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGaa                                                               >  1:2019928/1‑90 (MQ=255)

                       agGTGATTTCCCATTGCCGGTTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGaaa                                                              <  1:179162/90‑1 (MQ=255)

                       agGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGaaa                                                              <  1:3159275/90‑1 (MQ=255)

                       agGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGaaa                                                              >  1:1537321/1‑90 (MQ=255)

                       agGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGaaa                                                              <  1:125059/90‑1 (MQ=255)

                        ggTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGaaaa                                                             >  2:2632664/1‑90 (MQ=255)

                          tGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACg                                                           >  2:2482336/1‑90 (MQ=255)

                           gATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGa                                                          >  1:2704334/1‑90 (MQ=255)

                            aTTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGAt                                                         >  2:787698/1‑90 (MQ=255)

                            aTTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGAt                                                         >  2:1209940/1‑90 (MQ=255)

                            aTTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGAt                                                         <  1:3322501/90‑1 (MQ=255)

                            aTTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGAt                                                         >  1:1770271/1‑90 (MQ=255)

                             tttCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATc                                                        >  1:3488353/1‑90 (MQ=255)

                              ttCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCt                                                       <  2:295534/90‑1 (MQ=255)

                               tCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTg                                                      <  1:245425/90‑1 (MQ=255)

                                cccATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTgg                                                     >  2:3189888/1‑90 (MQ=255)

                                 ccATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGc                                                    <  1:2964401/90‑1 (MQ=255)

                                 ccATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGc                                                    <  1:2559598/90‑1 (MQ=255)

                                 ccATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGc                                                    <  1:2812356/90‑1 (MQ=255)

                                  cATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCa                                                   <  1:2776436/90‑1 (MQ=255)

                                     tGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCAttt                                                <  1:2985065/90‑1 (MQ=255)

                                      gCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCAtttt                                               <  2:1450794/90‑1 (MQ=255)

                                      gCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCAtttt                                               >  1:570787/1‑90 (MQ=255)

                                        cGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTAt                                             >  1:1706921/1‑90 (MQ=255)

                                         gggTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTAtt                                            >  2:2543466/1‑90 (MQ=255)

                                             tAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCt                                        <  1:2185482/90‑1 (MQ=255)

                                              aaTTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCtt                                       <  2:1039822/90‑1 (MQ=255)

                                               aTTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTg                                      <  2:1520063/90‑1 (MQ=255)

                                                ttATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGa                                     >  1:579054/1‑90 (MQ=255)

                                                 tATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGAt                                    >  2:2673905/1‑90 (MQ=255)

                                                 tATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGAt                                    >  1:673032/1‑90 (MQ=255)

                                                  aTCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATg                                   >  2:2911075/1‑90 (MQ=255)

                                                  aTCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATg                                   >  1:1738957/1‑90 (MQ=255)

                                                    cGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTc                                 >  1:3231802/1‑90 (MQ=255)

                                                    cGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTc                                 <  1:3065488/90‑1 (MQ=255)

                                                     gTCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCa                                <  2:218769/90‑1 (MQ=255)

                                                      tCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCAc                               <  1:1799920/90‑1 (MQ=255)

                                                      tCAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCAc                               >  2:2480070/1‑90 (MQ=255)

                                                       cAATTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCAcc                              <  2:3110375/90‑1 (MQ=255)

                                                        aaTTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCAccc                             >  2:1638259/1‑90 (MQ=255)

                                                        aaTTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCAccc                             >  1:3143489/1‑90 (MQ=255)

                                                         aTTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTTACCCg                            <  2:495394/90‑1 (MQ=255)

                                                         aTTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCg                            <  2:1985463/90‑1 (MQ=255)

                                                         aTTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCg                            >  2:1254319/1‑90 (MQ=255)

                                                         aTTGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCg                            <  1:2193942/90‑1 (MQ=255)

                                                          ttGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGa                           <  2:1956260/90‑1 (MQ=255)

                                                          ttGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGa                           <  1:1442240/90‑1 (MQ=255)

                                                           tGGTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAg                          >  2:1919452/1‑90 (MQ=255)

                                                            ggTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGc                         >  2:1053346/1‑90 (MQ=255)

                                                             gTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCt                        >  1:3350066/1‑90 (MQ=255)

                                                             gTTGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCt                        >  1:372026/1‑90 (MQ=255)

                                                               tGCCGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTgg                      >  1:1750246/1‑90 (MQ=255)

                                                                 ccGCGGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGAc                    <  1:193644/90‑1 (MQ=255)

                                                                   gcgGACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACAt                  >  1:433625/1‑90 (MQ=255)

                                                                     ggACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATaa                >  2:3082682/1‑90 (MQ=255)

                                                                      gACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATTTTACCCGAGCTGGACATAAt               <  2:3091853/90‑1 (MQ=255)

                                                                      gACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAAt               <  1:612187/90‑1 (MQ=255)

                                                                      gACAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAAt               <  2:1303445/90‑1 (MQ=255)

                                                                       aCAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATg              <  2:2493209/90‑1 (MQ=255)

                                                                       aCAAACCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATg              >  1:28264/1‑90 (MQ=255)

                                                                         aaaCCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGc            >  2:1032024/1‑90 (MQ=255)

                                                                         aaaCCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGc            <  2:730530/90‑1 (MQ=255)

                                                                         aaaCCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGc            >  2:3024300/1‑90 (MQ=255)

                                                                         aaaCCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGc            <  1:1538522/90‑1 (MQ=255)

                                                                         aaaCCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGc            >  2:3315016/1‑90 (MQ=255)

                                                                          aaCCCGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCg           >  1:2289398/1‑90 (MQ=255)

                                                                            cccGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTa         <  1:2542845/90‑1 (MQ=255)

                                                                            cccGGCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTa         >  1:278700/1‑90 (MQ=255)

                                                                               ggCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACtt      >  2:1332482/1‑90 (MQ=255)

                                                                               ggCCTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGATGGACATAATGGCGTACtt      >  1:1571221/1‑90 (MQ=255)

                                                                                 ccTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTtgc    <  2:752166/90‑1 (MQ=255)

                                                                                  cTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTtgct   <  1:2277910/90‑1 (MQ=255)

                                                                                  cTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTtgct   <  1:405390/90‑1 (MQ=255)

                                                                                  cTGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGAGTACTtgct   <  1:1886615/90‑1 (MQ=255)

                                                                                   tGGCGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCCCCCGAGCTGGACATAATGGCGTACTtgctg  <  1:1087242/90‑1 (MQ=255)

GGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTG  >  AC_000091/1292292‑1292464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`