BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,303,712	A→G	S273G (AGC→GGC)	oppA →	oligopeptide transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,303,712	0	A	G	100.0%	522.3 / NA	133	S273G (AGC→GGC)	oppA	oligopeptide transporter subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (73/60); total (73/60)

Only 100 of 133 total aligned reads displayed.

TTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTAC  >  AC_000091/1303626‑1303798

ttaACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCa                                                                                     <  2:2270757/90‑1 (MQ=255)

 taACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGACATCGACATGACGAATAACGGCAt                                                                                    >  2:3461172/1‑90 (MQ=255)

 taACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCAt                                                                                    <  2:2133051/90‑1 (MQ=255)

  aaCCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATg                                                                                   <  2:2322550/90‑1 (MQ=255)

    ccAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGcc                                                                                 <  1:1237010/90‑1 (MQ=255)

      aGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGa                                                                               >  1:1179721/1‑90 (MQ=255)

      aGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGa                                                                               >  1:789946/1‑90 (MQ=255)

       ggTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGAt                                                                              >  1:1442675/1‑90 (MQ=255)

          aaCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGa                                                                           >  2:1322961/1‑90 (MQ=255)

          aaCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGa                                                                           >  1:867541/1‑90 (MQ=255)

            ccTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGCGAAATCGACATGACGAATAACGGCATGCCGATCGAAt                                                                         >  1:3279563/1‑90 (MQ=255)

             cTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAAtt                                                                        >  1:2338136/1‑90 (MQ=255)

             cTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAAtt                                                                        >  1:3434058/1‑90 (MQ=255)

             cTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAAtt                                                                        >  1:2414204/1‑90 (MQ=255)

             cTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAAtt                                                                        >  1:653164/1‑90 (MQ=255)

             cTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAAtt                                                                        <  2:1037211/90‑1 (MQ=255)

               aTTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGt                                                                      >  1:2915636/1‑90 (MQ=255)

               aTTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGt                                                                      >  2:1263604/1‑90 (MQ=255)

                tttGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGtt                                                                     >  1:2944151/1‑90 (MQ=255)

                 ttGCCGATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTc                                                                    <  2:2767312/90‑1 (MQ=255)

                  tGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTcc                                                                   <  1:2099275/90‑1 (MQ=255)

                  tGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTcc                                                                   >  1:1599254/1‑90 (MQ=255)

                   gCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCa                                                                  <  2:1757158/90‑1 (MQ=255)

                    ccTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAg                                                                 >  2:1878310/1‑90 (MQ=255)

                     cTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGa                                                                >  2:2602764/1‑90 (MQ=255)

                     cTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGa                                                                >  2:3115902/1‑90 (MQ=255)

                     cTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGa                                                                <  1:1003181/90‑1 (MQ=255)

                      tATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGaa                                                               <  2:488449/90‑1 (MQ=255)

                       aTTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAg                                                              <  2:1087973/90‑1 (MQ=255)

                       aTTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAg                                                              <  1:1993710/90‑1 (MQ=255)

                        ttGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGc                                                             <  1:2403931/90‑1 (MQ=255)

                         tGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCt                                                            >  2:1172182/1‑90 (MQ=255)

                         tGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCt                                                            >  2:3371941/1‑90 (MQ=255)

                          gCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTg                                                           <  2:3168520/90‑1 (MQ=255)

                           cTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGa                                                          >  2:653349/1‑90 (MQ=255)

                             tCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGaaa                                                        >  1:1889853/1‑90 (MQ=255)

                              cTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGaaaa                                                       >  1:3162547/1‑90 (MQ=255)

                               tGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGaaaaa                                                      >  1:840246/1‑90 (MQ=255)

                                 aaGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAag                                                    >  2:2492309/1‑90 (MQ=255)

                                 aaGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAag                                                    >  1:2002256/1‑90 (MQ=255)

                                  aGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAaga                                                   <  2:1354607/90‑1 (MQ=255)

                                  aGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAaga                                                   >  1:2686529/1‑90 (MQ=255)

                                   gTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAagag                                                  >  1:937349/1‑90 (MQ=255)

                                    ttACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAgaga                                                 >  2:783767/1‑90 (MQ=255)

                                     ttccGAGGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGAt                                                <  2:2471359/88‑1 (MQ=255)

                                     tACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGAt                                                >  1:354750/1‑90 (MQ=255)

                                     tACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGAt                                                >  2:2970979/1‑90 (MQ=255)

                                     tACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGAt                                                <  2:1026697/90‑1 (MQ=255)

                                        cGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATccc                                             <  1:1506506/90‑1 (MQ=255)

                                        cGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATccc                                             >  1:1955056/1‑90 (MQ=255)

                                         gATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCg                                            <  2:1888095/90‑1 (MQ=255)

                                          aTGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCgg                                           >  1:1082847/1‑90 (MQ=255)

                                          aTGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCgg                                           >  1:17068/1‑90 (MQ=255)

                                            gTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGAc                                         <  2:3164309/90‑1 (MQ=255)

                                             tCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCTAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACg                                        <  2:694442/90‑1 (MQ=255)

                                             tCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACg                                        <  2:2287174/90‑1 (MQ=255)

                                              cAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGa                                       >  2:815354/1‑90 (MQ=255)

                                               aaCCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGaa                                      <  2:866507/90‑1 (MQ=255)

                                               aaCCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGaa                                      >  1:2547729/1‑90 (MQ=255)

                                                 ccGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGt                                    <  2:2448815/90‑1 (MQ=255)

                                                   gCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGACGCTGAAAAAAGAGATCCCGGACGAAGTTc                                  <  2:1315225/90‑1 (MQ=255)

                                                    cTACCTTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCa                                 >  2:1470653/1‑90 (MQ=255)

                                                    cTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCa                                 >  2:2892005/1‑90 (MQ=255)

                                                     tACCGTAGTGGTGAAATCGACCTGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCAc                                <  2:2213491/90‑1 (MQ=255)

                                                       ccGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGt                              >  1:3403462/1‑90 (MQ=255)

                                                       ccGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGNNCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGt                              >  2:560852/1‑90 (MQ=255)

                                                        cGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGtt                             >  2:412590/1‑90 (MQ=255)

                                                        cGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGtt                             >  1:1667358/1‑90 (MQ=255)

                                                        cGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGtt                             <  2:1042003/90‑1 (MQ=255)

                                                         gTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTg                            >  2:299876/1‑90 (MQ=255)

                                                         gTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTg                            <  2:1540606/90‑1 (MQ=255)

                                                          tAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGa                           <  1:2281084/90‑1 (MQ=255)

                                                           aGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGAt                          >  1:3336459/1‑90 (MQ=255)

                                                           aGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGAt                          >  2:1371369/1‑90 (MQ=255)

                                                           aGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGAt                          >  2:3033182/1‑90 (MQ=255)

                                                             tggtgAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATcc                        <  1:975105/90‑1 (MQ=255)

                                                              ggtgAAATCGACATGACGAATAACGGCATGCCGATCNANNNGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCa                       <  2:2084358/90‑1 (MQ=255)

                                                                 gAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAc                    <  1:3056000/90‑1 (MQ=255)

                                                                  aaaTCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                   >  1:1902863/1‑90 (MQ=255)

                                                                  aaaTCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                   >  1:3187171/1‑90 (MQ=255)

                                                                   aaTCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCt                  <  2:1143159/90‑1 (MQ=255)

                                                                     tCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtgt                <  2:527544/90‑1 (MQ=255)

                                                                     tCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtgt                <  2:627997/90‑1 (MQ=255)

                                                                     tCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtgt                >  1:178509/1‑90 (MQ=255)

                                                                      cGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtgtg               >  1:754346/1‑90 (MQ=255)

                                                                       gACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGc              <  1:1163649/90‑1 (MQ=255)

                                                                       gACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGc              >  1:902365/1‑90 (MQ=255)

                                                                         cATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc            >  1:3367594/1‑90 (MQ=255)

                                                                         cATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc            >  2:3450438/1‑90 (MQ=255)

                                                                         cATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc            >  1:1001913/1‑90 (MQ=255)

                                                                         cATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc            <  2:37656/90‑1 (MQ=255)

                                                                          aTGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACt           >  1:747336/1‑90 (MQ=255)

                                                                          aTGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACt           <  1:1546647/90‑1 (MQ=255)

                                                                            gACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAGAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTa         >  2:450751/1‑90 (MQ=255)

                                                                            gACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTa         <  1:1996523/90‑1 (MQ=255)

                                                                            gACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTa         <  1:941727/90‑1 (MQ=255)

                                                                             aCGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTAc        <  2:1534124/90‑1 (MQ=255)

                                                                                aaTAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTAt     >  1:3405840/1‑90 (MQ=255)

                                                                                  tAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTa   <  1:2074304/90‑1 (MQ=255)

                                                                                   aaCGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTAACGTTGATCCATACCTAGGCACTTACTATTAc  >  2:1390135/1‑90 (MQ=255)

TTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTAC  >  AC_000091/1303626‑1303798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`