BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,341,506	A→G	T155A (ACG→GCG)	pgpB →	phosphatidylglycerophosphate phosphatase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,341,506	0	A	G	100.0%	464.4 / NA	120	T155A (ACG→GCG)	pgpB	phosphatidylglycerophosphate phosphatase B
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (77/43); total (77/43)

Only 100 of 123 total aligned reads displayed.

CGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGC  >  AC_000091/1341419‑1341590

cGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCg                                                                                    <  1:1339465/90‑1 (MQ=255)

 gAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCgg                                                                                   <  2:2256545/90‑1 (MQ=255)

 gAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCgg                                                                                   >  1:2058334/1‑90 (MQ=255)

  aGCAGAACGCCGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCggg                                                                                  <  2:2255131/90‑1 (MQ=255)

    cAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGt                                                                                >  1:3435080/1‑90 (MQ=255)

    cAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGt                                                                                >  2:3027449/1‑90 (MQ=255)

    cAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGt                                                                                >  1:175445/1‑90 (MQ=255)

     aGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGtt                                                                               <  2:78069/90‑1 (MQ=255)

      gAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGttt                                                                              <  2:1446280/90‑1 (MQ=255)

       aaCGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTg                                                                             <  1:1582800/90‑1 (MQ=255)

       aaCGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTg                                                                             >  1:3453195/1‑90 (MQ=255)

         cgcgGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGcc                                                                           >  1:899428/1‑90 (MQ=255)

         cgcgGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGATTGcc                                                                           >  1:1459185/1‑90 (MQ=255)

          gcgGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCt                                                                          <  2:2901193/90‑1 (MQ=255)

           cgGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCtt                                                                         <  1:1406996/90‑1 (MQ=255)

           cgGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCtt                                                                         >  2:310447/1‑90 (MQ=255)

              aaaTCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTttcc                                                                      <  2:2433185/90‑1 (MQ=255)

              aaaTCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGGTTGCCTttcc                                                                      >  1:1060068/1‑90 (MQ=255)

              aaaTCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGGTTGCCTttcc                                                                      >  2:2237008/1‑90 (MQ=255)

               aaTCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTAGCCTttcct                                                                     >  2:1931070/1‑90 (MQ=255)

               aaTCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGGTTGCCTttcct                                                                     >  1:2520490/1‑90 (MQ=255)

                aTCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTttcctt                                                                    >  1:1406279/1‑90 (MQ=255)

                aTCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTttcctt                                                                    <  1:2908723/90‑1 (MQ=255)

                 tCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTttccttc                                                                   <  1:1117596/90‑1 (MQ=255)

                  cTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTttccttcc                                                                  <  2:2239876/90‑1 (MQ=255)

                   tAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCCCAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCg                                                                 <  1:294629/90‑1 (MQ=255)

                   tAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCg                                                                 >  2:608607/1‑90 (MQ=255)

                     gTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGt                                                               >  1:2238647/1‑90 (MQ=255)

                     gTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGt                                                               >  1:2154282/1‑90 (MQ=255)

                     gTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGt                                                               <  1:1206311/90‑1 (MQ=255)

                       gAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTca                                                             >  2:439649/1‑90 (MQ=255)

                         aaGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTcaca                                                           >  2:1355869/1‑90 (MQ=255)

                         aaGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTcaca                                                           >  2:1066877/1‑90 (MQ=255)

                          aGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCacac                                                          <  2:1623197/90‑1 (MQ=255)

                           gAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACg                                                         <  1:2938582/90‑1 (MQ=255)

                           gAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGGTTGCCTTTTCTTCCGGTCACACg                                                         >  2:403851/1‑90 (MQ=255)

                            aaCAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGa                                                        >  2:1325420/1‑90 (MQ=255)

                             aCAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGAt                                                       >  2:678576/1‑90 (MQ=255)

                             aCAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGAt                                                       <  1:687743/90‑1 (MQ=255)

                               aGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGt                                                     >  1:555246/1‑90 (MQ=255)

                                gTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGtt                                                    >  1:2816720/1‑90 (MQ=255)

                                gTTGGCGGAAGAGAAAAATGTCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGAGGtt                                                    >  2:2138141/1‑90 (MQ=255)

                                  tGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCTCACTGGCAGAAAGAGGCGGGGGTTGCCTTTCCTTCCGGTCACACGATGGTtg                                                  >  2:689444/1‑90 (MQ=255)

                                  tGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTtg                                                  >  2:1818226/1‑90 (MQ=255)

                                    gCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTtgct                                                >  1:2634370/1‑90 (MQ=255)

                                      tGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTtgctgc                                              >  1:355195/1‑90 (MQ=255)

                                      tGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTtgctgc                                              >  1:2294739/1‑90 (MQ=255)

                                       gAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGcc                                             >  2:1813189/1‑90 (MQ=255)

                                       gAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGGCACACGATGTTTGCTGcc                                             >  1:1226869/1‑90 (MQ=255)

                                        aaGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAATGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCa                                            >  2:2673273/1‑90 (MQ=255)

                                        aaGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCa                                            >  2:2949171/1‑90 (MQ=255)

                                         agagAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAg                                           >  2:3275475/1‑90 (MQ=255)

                                          gagaAAAATATCCCACAATATTTGCATTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGt                                          >  1:860645/1‑90 (MQ=255)

                                           agaAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGtt                                         <  2:2146089/90‑1 (MQ=255)

                                           agaAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGtt                                         >  1:1947586/1‑90 (MQ=255)

                                           agaAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGtt                                         >  2:2875310/1‑90 (MQ=255)

                                              aaaaTATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGTGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTggg                                      >  1:2611787/1‑90 (MQ=255)

                                               aaaTATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGc                                     >  2:2284138/1‑90 (MQ=255)

                                               aaaTATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGGCACACGCTGTTTGCTGCCAGTTggga                                     >  2:1210086/1‑89 (MQ=255)

                                                 atatCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCAc                                   >  1:2477421/1‑90 (MQ=255)

                                                 atatCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGCCAc                                   <  2:2387740/90‑1 (MQ=255)

                                                 atatCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGGTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCAc                                   >  2:1279390/1‑90 (MQ=255)

                                                  tatCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCAct                                  <  2:735544/90‑1 (MQ=255)

                                                   atCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCActg                                 >  1:176783/1‑90 (MQ=255)

                                                    tCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCActgc                                >  2:1575972/1‑90 (MQ=255)

                                                    tCCCACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGGCACACGATGTTTGCCGCCAGTTGGGCActgc                                >  1:186087/1‑90 (MQ=255)

                                                      ccACAATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCActgctg                              >  1:279632/1‑90 (MQ=255)

                                                       cacaATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTgg                             >  2:132764/1‑90 (MQ=255)

                                                        acaATATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGc                            >  1:891525/1‑90 (MQ=255)

                                                          aaTATTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCg                          >  2:1194161/1‑90 (MQ=255)

                                                            tatTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGtt                        >  2:147280/1‑90 (MQ=255)

                                                            tatTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGtt                        <  2:1838017/90‑1 (MQ=255)

                                                             atTTGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTg                       >  1:663780/1‑90 (MQ=255)

                                                              tttGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGGTTGCTTCCAGTTGGGCACTGCTGGCCGTTgg                      >  2:2193631/1‑90 (MQ=255)

                                                              tttGCGTTCACACTGGCAGAAAGAGGCGGGGGTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTgg                      >  2:1264917/1‑90 (MQ=255)

                                                               ttGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGt                     <  1:539449/90‑1 (MQ=255)

                                                                tGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTTCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGtt                    >  1:3390053/1‑90 (MQ=255)

                                                                tGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGtt                    >  2:3315458/1‑90 (MQ=255)

                                                                tGCGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGtt                    <  2:656878/90‑1 (MQ=255)

                                                                  cGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTg                  >  1:2825453/1‑90 (MQ=255)

                                                                  cGTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTg                  <  2:3149268/90‑1 (MQ=255)

                                                                   gTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGc                 >  1:2453726/1‑90 (MQ=255)

                                                                   gTTCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGc                 >  2:1439488/1‑90 (MQ=255)

                                                                     tCACACTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCtg               >  1:2933840/1‑90 (MQ=255)

                                                                      cacaCTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCtgt              >  2:212400/1‑90 (MQ=255)

                                                                       acacTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCtgtg             <  1:2504410/90‑1 (MQ=255)

                                                                         acTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGc           >  1:45550/1‑90 (MQ=255)

                                                                         acTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGc           >  1:116864/1‑90 (MQ=255)

                                                                          cTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGcc          <  1:350864/90‑1 (MQ=255)

                                                                          cTGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGcc          <  1:617604/90‑1 (MQ=255)

                                                                           tGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCcg         <  2:1321250/90‑1 (MQ=255)

                                                                           tGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCcg         <  1:3212454/90‑1 (MQ=255)

                                                                           tGGCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCcg         >  1:463191/1‑90 (MQ=255)

                                                                           aggCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCcg         >  2:907097/2‑90 (MQ=255)

                                                                            ggCAGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCcgc        >  1:990495/1‑90 (MQ=255)

                                                                               aGAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTc     <  2:107585/90‑1 (MQ=255)

                                                                                gAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCg    <  1:1667319/90‑1 (MQ=255)

                                                                                gAAAGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCg    <  2:957571/90‑1 (MQ=255)

                                                                                 aaaGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCgg   >  1:1892165/1‑90 (MQ=255)

                                                                                  aaGAGGCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGc  <  1:1401791/90‑1 (MQ=255)

CGAGCAGAACGCGGAAATCTAGTGAAAGAACAGTTGGCTGAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGC  >  AC_000091/1341419‑1341590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`