BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,369,160	A→G	V156A (GTC→GCC)	pspF ←	DNA‑binding transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,369,160	0	A	G	100.0%	451.1 / NA	116	V156A (GTC→GCC)	pspF	DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (79/37); total (79/37)

Only 100 of 116 total aligned reads displayed.

TCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGACCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAACGCGCTCCAGTTC  >  AC_000091/1369073‑1369245

tCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGcc                                                                                     >  2:2849042/1‑90 (MQ=255)

tCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGcc                                                                                     >  2:3145197/1‑90 (MQ=255)

tCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGcc                                                                                     >  1:1268602/1‑90 (MQ=255)

 cGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCa                                                                                    >  2:2016447/1‑90 (MQ=255)

  gCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCAt                                                                                   >  1:772368/1‑90 (MQ=255)

  gCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCAt                                                                                   >  1:173388/1‑90 (MQ=255)

    tttCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCg                                                                                 <  1:1176536/90‑1 (MQ=255)

     ttCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGc                                                                                >  1:3202695/1‑90 (MQ=255)

      tcgcgctcgcgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGcc                                                                               <  2:1752062/90‑1 (MQ=255)

      tcgcgctcgcgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGcc                                                                               >  2:3175139/1‑90 (MQ=255)

      tcgcgctcgcgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGcc                                                                               <  2:1326969/90‑1 (MQ=255)

         cgctcgcgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCggg                                                                            <  2:1160508/90‑1 (MQ=255)

         cgctcgcgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCggg                                                                            >  1:2028651/1‑90 (MQ=255)

          gctcgcgcAGTGGAGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGga                                                                           >  2:186661/1‑90 (MQ=255)

           ctcgcgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGgag                                                                          >  2:1569277/1‑90 (MQ=255)

            tcgcgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGgaga                                                                         >  2:1524504/1‑90 (MQ=255)

             cgcgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGAt                                                                        >  1:3284865/1‑90 (MQ=255)

              gcgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTGATTGGCCATCGCCGGGAGATc                                                                       >  2:2313437/1‑90 (MQ=255)

               cgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGga                                                                           >  2:2364605/1‑85 (MQ=255)

               cgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGga                                                                           <  1:2364605/85‑1 (MQ=255)

               cgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCg                                                                      <  2:3288806/90‑1 (MQ=255)

               cgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCg                                                                      <  2:656209/90‑1 (MQ=255)

               cgcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCg                                                                      >  2:811574/1‑90 (MQ=255)

                gcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCgg                                                                     >  2:3289190/1‑90 (MQ=255)

                gcAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCgg                                                                     >  1:3309113/1‑90 (MQ=255)

                   gtggtgGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCAt                                                                  >  2:1305843/1‑90 (MQ=255)

                    tggttgCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCAtt                                                                 <  2:1207757/90‑1 (MQ=255)

                    tggtggCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCAtt                                                                 <  2:2808472/90‑1 (MQ=255)

                    tggtggCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCAtt                                                                 >  1:1302653/1‑90 (MQ=255)

                    tggtggCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCAtt                                                                 >  1:2137987/1‑90 (MQ=255)

                     ggtggCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATtc                                                                <  1:651594/90‑1 (MQ=255)

                     ggtggCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATtc                                                                <  2:1326227/90‑1 (MQ=255)

                     ggtggCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATtc                                                                <  2:1361273/90‑1 (MQ=255)

                     ggtggCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATtc                                                                >  2:663682/1‑90 (MQ=255)

                      gtggCAGTTGTACAACATCAAAAGCCCGTCGGTCGAGCAGGCCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGCTCGGCATtcg                                                               >  2:2465240/1‑90 (MQ=255)

                      gtggCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATtcg                                                               >  1:1465984/1‑90 (MQ=255)

                       tggCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATtcgt                                                              >  2:3350445/1‑90 (MQ=255)

                        ggCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATtcgtc                                                             <  1:534486/90‑1 (MQ=255)

                        ggCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATtcgtc                                                             <  1:2335603/90‑1 (MQ=255)

                          cAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTcgc                                                           >  1:1377256/1‑90 (MQ=255)

                           aGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTcgcg                                                          >  1:1872197/1‑90 (MQ=255)

                             ttGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCa                                                        <  2:2935587/90‑1 (MQ=255)

                              tGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCAt                                                       <  2:2823169/90‑1 (MQ=255)

                                  caacaTCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATATCa                                                   >  1:278429/1‑90 (MQ=255)

                                   aacaTCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCaa                                                  >  2:3377365/1‑90 (MQ=255)

                                    acaTCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAAc                                                 <  1:3242777/90‑1 (MQ=255)

                                    acaTCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAAc                                                 >  2:2777356/1‑90 (MQ=255)

                                     caTCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAAcc                                                <  1:3272922/90‑1 (MQ=255)

                                     caTCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAAcc                                                >  1:1961444/1‑90 (MQ=255)

                                     caTCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAAcc                                                <  1:3152603/90‑1 (MQ=255)

                                      aTCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCg                                               <  1:2597668/90‑1 (MQ=255)

                                         aaaaGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGcac                                            <  2:2245034/90‑1 (MQ=255)

                                         aaaaGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGcac                                            >  2:3118037/1‑90 (MQ=255)

                                         aaaaGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGcac                                            >  2:1944752/1‑90 (MQ=255)

                                            aGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACAtt                                         <  1:2098536/90‑1 (MQ=255)

                                              ccAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCa                                       >  1:3094570/1‑90 (MQ=255)

                                               cAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCAc                                      >  1:1838804/1‑90 (MQ=255)

                                               cAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCAc                                      >  2:3472952/1‑90 (MQ=255)

                                               cAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCAc                                      >  1:1164679/1‑90 (MQ=255)

                                                aGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCAcc                                     >  1:1182024/1‑90 (MQ=255)

                                                 gtcggtcgAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCt                                    <  2:514448/90‑1 (MQ=255)

                                                  tcggtcgAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTg                                   <  2:2073787/90‑1 (MQ=255)

                                                   cggtcgAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGc                                  >  1:1397866/1‑90 (MQ=255)

                                                    ggtcgAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCa                                 >  2:2326772/1‑90 (MQ=255)

                                                    ggtcgAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCa                                 >  2:2128070/1‑90 (MQ=255)

                                                        gAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATgg                             >  2:236895/1‑90 (MQ=255)

                                                        gAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATgg                             >  1:1026162/1‑90 (MQ=255)

                                                        gAGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATgg                             >  2:2098206/1‑90 (MQ=255)

                                                         aGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGt                            <  1:131443/90‑1 (MQ=255)

                                                         aGCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGt                            >  2:2532129/1‑90 (MQ=255)

                                                          gCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGtt                           <  2:2054285/90‑1 (MQ=255)

                                                          gCAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGtt                           <  2:1820200/90‑1 (MQ=255)

                                                          gCAGGTCAGCGCGAAAAGCGCCTTCATTAGCCATCGCCGGGAGATCGGCATTCGTGGCGCATACCAACCGCACATTCACCTGCAATGGtt                           >  2:587229/1‑90 (MQ=255)

                                                           cAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCGTTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTg                          >  2:620281/1‑90 (MQ=255)

                                                           cAGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTg                          >  2:2792884/1‑90 (MQ=255)

                                                            aGGTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTgg                         >  2:2827917/1‑90 (MQ=255)

                                                             ggTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGc                        >  1:1681887/1‑90 (MQ=255)

                                                             ggTCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGc                        >  2:804972/1‑90 (MQ=255)

                                                               tCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTg                      <  2:2420927/90‑1 (MQ=255)

                                                               tCAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTg                      >  1:2291186/1‑90 (MQ=255)

                                                                cAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTgc                     >  1:1077824/1‑90 (MQ=255)

                                                                cAGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTgc                     <  1:3302991/90‑1 (MQ=255)

                                                                 aGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTgcc                    >  2:3287872/1‑90 (MQ=255)

                                                                 aGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTgcc                    >  2:1598158/1‑90 (MQ=255)

                                                                 aGCGCGAAAAGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTgcc                    <  1:2747249/90‑1 (MQ=255)

                                                                       aaaaGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAAc              >  2:1674341/1‑90 (MQ=255)

                                                                       aaaaGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAAc              >  2:1501880/1‑90 (MQ=255)

                                                                       aaaaGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAAc              >  1:705106/1‑90 (MQ=255)

                                                                       aaaaGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAAc              >  1:699095/1‑90 (MQ=255)

                                                                        aaaGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGGCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAAcg             >  1:596407/1‑90 (MQ=255)

                                                                         aaGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAAcgc            >  1:2725485/1‑90 (MQ=255)

                                                                          aGTGCCTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAAcgcg           >  2:1607794/1‑90 (MQ=255)

                                                                              ccTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAACGCGCTcc       >  2:2524344/1‑90 (MQ=255)

                                                                               cTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAACGCGCTCCa      >  1:114157/1‑90 (MQ=255)

                                                                               cTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAACGCGCTCCa      <  1:1127968/90‑1 (MQ=255)

                                                                               cTTCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAACGCGCTCCa      <  1:1656270/90‑1 (MQ=255)

                                                                                ttCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAACGCGCTCCAg     >  1:2685250/1‑90 (MQ=255)

                                                                                 tCATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAACGCGCTCCAGt    >  2:1948673/1‑90 (MQ=255)

                                                                                  cATTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAACGCGCTCCAGtt   >  2:382209/1‑90 (MQ=255)

                                                                                   aTTGGCCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAACGCGCTCCAGTTc  >  2:3400531/1‑90 (MQ=255)

TCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCAGCGCGAAAAGTGCCTTCATTGACCATCGCCGGGAGATCGGCATTCGTCGCGCATACCAACCGCACATTCACCTGCAATGGTTGGCTGCCGCCAACGCGCTCCAGTTC  >  AC_000091/1369073‑1369245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`