BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,609,104	A→G	E15E (GAA→GAG)	tam →	trans‑aconitate methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,609,104	0	A	G	100.0%	431.8 / NA	110	E15E (GAA→GAG)	tam	trans‑aconitate methyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (52/58); total (52/58)

Only 100 of 114 total aligned reads displayed.

ATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAA  >  AC_000091/1609017‑1609190

aTTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCg                                                                                      >  2:2115092/1‑90 (MQ=255)

aTTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCg                                                                                      >  2:3380390/1‑90 (MQ=255)

 ttttATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGa                                                                                     >  2:2361394/1‑90 (MQ=255)

  tttATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTGCGCTTTTCCGCTGAGCGAt                                                                                    <  2:2455559/90‑1 (MQ=255)

  tttATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGAt                                                                                    <  2:3334359/90‑1 (MQ=255)

  tttATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGAt                                                                                    <  1:2052479/90‑1 (MQ=255)

   ttATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATc                                                                                   >  1:52337/1‑90 (MQ=255)

   ttATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATc                                                                                   <  1:1639130/90‑1 (MQ=255)

     aTCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGACCCCCTCTTTATATCTACACTTTTCCGCTGAGCGATcgc                                                                                 <  1:4289/90‑1 (MQ=255)

      tCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGCCTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATcgcg                                                                                <  2:535480/90‑1 (MQ=255)

      tCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATcgcg                                                                                >  2:3323316/1‑90 (MQ=255)

       cTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGt                                                                               >  2:164594/1‑90 (MQ=255)

        tACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTc                                                                              <  1:1209530/90‑1 (MQ=255)

        tACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTc                                                                              <  2:2786943/90‑1 (MQ=255)

         aCAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTcc                                                                             <  1:1352839/90‑1 (MQ=255)

          cAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcg                                                                            >  1:1966169/1‑90 (MQ=255)

          cAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcg                                                                            >  2:3096486/1‑90 (MQ=255)

          cAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcg                                                                            >  2:417551/1‑90 (MQ=255)

          cAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcg                                                                            <  1:1426084/90‑1 (MQ=255)

          cAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCACTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcg                                                                            <  2:2675477/90‑1 (MQ=255)

           aaTTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCNTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcgg                                                                           >  2:3248159/1‑90 (MQ=255)

           aaTTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcgg                                                                           <  1:1748196/90‑1 (MQ=255)

           aaTTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcgg                                                                           >  1:2201178/1‑90 (MQ=255)

           aaTTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcgg                                                                           >  2:1192317/1‑90 (MQ=255)

            aTTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcggc                                                                          >  1:1543200/1‑90 (MQ=255)

             ttGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcggcg                                                                         <  1:1311078/90‑1 (MQ=255)

             ttGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCcggcg                                                                         >  2:686923/1‑90 (MQ=255)

               ggggTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGt                                                                       <  1:3345611/90‑1 (MQ=255)

               ggggTAACGCGCTGACGGGAGGAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGt                                                                       >  2:2144842/1‑90 (MQ=255)

                gggTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTg                                                                      <  2:1095733/90‑1 (MQ=255)

                gggTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTg                                                                      >  1:961449/1‑90 (MQ=255)

                gggTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTg                                                                      <  1:897381/90‑1 (MQ=255)

                  gTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGa                                                                    <  2:2764135/90‑1 (MQ=255)

                   tAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAg                                                                   >  1:2926741/1‑90 (MQ=255)

                   tAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAg                                                                   <  1:953044/90‑1 (MQ=255)

                   tAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAg                                                                   >  1:5514/1‑90 (MQ=255)

                     aCGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAgct                                                                 <  1:1916531/90‑1 (MQ=255)

                      cgcgCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAgctg                                                                >  2:1078242/1‑90 (MQ=255)

                        cgcTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAgctgct                                                              >  1:1802601/1‑90 (MQ=255)

                        cgcTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAgctgct                                                              >  2:1830708/1‑90 (MQ=255)

                         gcTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCtt                                                             <  2:2567442/90‑1 (MQ=255)

                         gcTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCtt                                                             <  1:2478206/90‑1 (MQ=255)

                         gcTGACGGGAGTAAAAAAAGGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCtt                                                             <  1:313448/90‑1 (MQ=255)

                          cTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTg                                                            <  2:1111592/90‑1 (MQ=255)

                             aCGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCa                                                         >  1:1765342/1‑90 (MQ=255)

                                  aGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTg                                                    >  2:2862874/1‑90 (MQ=255)

                                    tAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGcc                                                  >  1:2445306/1‑90 (MQ=255)

                                         aaaTGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTgg                                             >  2:2692159/1‑90 (MQ=255)

                                         aaaTGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTgg                                             >  1:457583/1‑90 (MQ=255)

                                          aaTGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGa                                            >  1:2264967/1‑90 (MQ=255)

                                          aaTGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGa                                            >  2:324292/1‑90 (MQ=255)

                                          aaTGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGa                                            <  2:2945263/90‑1 (MQ=255)

                                             gTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGaaaa                                         <  1:2867496/90‑1 (MQ=255)

                                                tGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGt                                      >  2:1452890/1‑90 (MQ=255)

                                                 gACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTc                                     <  2:2684325/90‑1 (MQ=255)

                                                  aCTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCg                                    <  2:107277/90‑1 (MQ=255)

                                                    tGGAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGaa                                  >  1:1313008/1‑90 (MQ=255)

                                                    tGGAACCCCTCTTTATATCTACACATTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGaa                                  <  1:3287172/90‑1 (MQ=255)

                                                     ggAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAat                                 >  1:2216562/1‑90 (MQ=255)

                                                      gAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAata                                >  1:2620007/1‑90 (MQ=255)

                                                      gAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAata                                <  1:1255013/90‑1 (MQ=255)

                                                      gAACCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAata                                >  2:3445002/1‑90 (MQ=255)

                                                       aaCCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAatat                               <  1:2410521/90‑1 (MQ=255)

                                                       aaCCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAatat                               <  2:229467/90‑1 (MQ=255)

                                                        aCCCCTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATg                              <  2:3319028/90‑1 (MQ=255)

                                                          cccTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGtt                            <  2:721923/90‑1 (MQ=255)

                                                          cccTCTTTACATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGtt                            <  2:3037517/90‑1 (MQ=255)

                                                           ccTCTTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTg                           <  2:1140151/90‑1 (MQ=255)

                                                            ctctTTATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGc                          <  1:2999354/90‑1 (MQ=255)

                                                              ctTTATATCTACACTTTTCCGCTGAGCGAGCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCg                        <  2:507591/90‑1 (MQ=255)

                                                                ttATATCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGAt                      >  1:796743/1‑90 (MQ=255)

                                                                 tataTCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATc                     <  2:2171689/90‑1 (MQ=255)

                                                                  atatCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATca                    <  2:212612/90‑2 (MQ=255)

                                                                   tatCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCtt                   >  1:3072583/1‑90 (MQ=255)

                                                                    atCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTg                  >  1:131632/1‑90 (MQ=255)

                                                                    atCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTg                  <  2:1651217/90‑1 (MQ=255)

                                                                     tCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTgg                 >  1:442892/1‑90 (MQ=255)

                                                                     tCTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTgg                 <  1:2426198/90‑1 (MQ=255)

                                                                      cTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGc                >  1:3053306/1‑90 (MQ=255)

                                                                      cTACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGc                >  2:380116/1‑90 (MQ=255)

                                                                       tACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCt               <  2:1382128/90‑1 (MQ=255)

                                                                       tACACTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCt               <  1:995771/90‑1 (MQ=255)

                                                                        acacTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCtg              >  2:1491271/1‑90 (MQ=255)

                                                                        acacTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCtg              <  2:3071341/90‑1 (MQ=255)

                                                                        acacTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTACCGATCTTGGCtg              <  2:2310329/90‑1 (MQ=255)

                                                                         cacTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCtgt             >  2:3005642/1‑90 (MQ=255)

                                                                         cacTTTTCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCtgt             <  2:1122540/90‑1 (MQ=255)

                                                                            ttttCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGc          <  2:1444609/90‑1 (MQ=255)

                                                                            ttttCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGc          >  1:1532983/1‑90 (MQ=255)

                                                                             tttCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGcc         >  1:1249186/1‑90 (MQ=255)

                                                                              ttCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGAAAAATGTCGAATATGTTACCGATCTTAGGTATGGccc        >  1:2273898/1‑90 (MQ=255)

                                                                               tCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCa       >  1:575099/1‑90 (MQ=255)

                                                                               tCCGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCa       >  1:1755767/1‑90 (MQ=255)

                                                                                ccGCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAg      <  1:744915/90‑1 (MQ=255)

                                                                                  gCTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCAAGGt    <  1:922791/90‑1 (MQ=255)

                                                                                   cTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTa   >  2:412459/1‑90 (MQ=255)

                                                                                   cTGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTa   <  1:2419428/90‑1 (MQ=255)

                                                                                    tGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTaa  <  2:264256/90‑1 (MQ=255)

                                                                                    tGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTaa  <  2:1068272/90‑1 (MQ=255)

                                                                                    tGAGCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTaa  >  1:687214/1‑90 (MQ=255)

ATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAA  >  AC_000091/1609017‑1609190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`