BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,644,094	T→C	intergenic (+313/+109)	cspF → / ← ydfT	cold shock protein/predicted antitermination protein Q

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,644,094	0	T	C	100.0%	459.1 / NA	116	intergenic (+313/+109)	cspF/ydfT	cold shock protein/predicted antitermination protein Q
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (57/59); total (57/59)

Only 100 of 119 total aligned reads displayed.

AATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATG  >  AC_000091/1644007‑1644179

aataatAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGctt                                                                                     >  1:2666080/1‑90 (MQ=255)

aataatAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGctt                                                                                     <  1:1923008/90‑1 (MQ=255)

   aataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCt                                                                                  >  1:2409130/1‑90 (MQ=255)

   aataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCt                                                                                  >  1:1177603/1‑90 (MQ=255)

     taaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTAc                                                                                >  2:1888876/1‑90 (MQ=255)

      aaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACt                                                                               <  1:1447444/90‑1 (MQ=255)

       aGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACtt                                                                              >  1:3406694/1‑90 (MQ=255)

       aGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACtt                                                                              <  1:1403959/90‑1 (MQ=255)

          tAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACttttt                                                                           <  2:3011504/90‑1 (MQ=255)

             cAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCtt                                                                        <  1:3396509/90‑1 (MQ=255)

             cAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCtt                                                                        >  1:1895282/1‑90 (MQ=255)

              aGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTa                                                                       >  1:2969534/1‑90 (MQ=255)

              aGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTa                                                                       >  1:3453134/1‑90 (MQ=255)

              aGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTa                                                                       <  2:1377985/90‑1 (MQ=255)

               gACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTaa                                                                      <  2:1990458/90‑1 (MQ=255)

                  aTGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGt                                                                   <  1:1366910/90‑1 (MQ=255)

                  aTGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGt                                                                   >  1:1073491/1‑90 (MQ=255)

                   tGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTc                                                                  <  2:296994/90‑1 (MQ=255)

                   tGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTc                                                                  <  1:22045/90‑1 (MQ=255)

                    gAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCg                                                                 >  2:3406363/1‑90 (MQ=255)

                     aGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCgg                                                                >  2:3029552/1‑90 (MQ=255)

                       tCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGt                                                              <  1:2177518/90‑1 (MQ=255)

                       tCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGt                                                              >  2:1780908/1‑90 (MQ=255)

                        cccGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGta                                                             >  2:194962/1‑90 (MQ=255)

                         ccGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGtat                                                            <  2:3295572/90‑1 (MQ=255)

                         ccGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGtat                                                            <  2:780791/90‑1 (MQ=255)

                          cGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGtata                                                           <  1:2076073/90‑1 (MQ=255)

                            ggATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACa                                                         >  1:3411581/1‑90 (MQ=255)

                             gATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTAGTTTTTCTTAACGTCGGGTATACaa                                                        >  2:3404802/1‑90 (MQ=255)

                             gATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACaa                                                        <  1:2243532/90‑1 (MQ=255)

                              aTGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACaaa                                                       <  1:3070731/90‑1 (MQ=255)

                               tGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAg                                                      <  2:1204886/90‑1 (MQ=255)

                                gagaTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGc                                                     >  2:1416162/1‑90 (MQ=255)

                                 agaTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGcc                                                    >  2:2935581/1‑90 (MQ=255)

                                 agaTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGcc                                                    <  1:1806175/90‑1 (MQ=255)

                                   aTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCa                                                  <  1:3221277/90‑1 (MQ=255)

                                   aTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCa                                                  >  1:494860/1‑90 (MQ=255)

                                    ttCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCAt                                                 >  2:3120236/1‑90 (MQ=255)

                                    ttCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCAt                                                 >  2:351067/1‑90 (MQ=255)

                                     tCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATc                                                <  2:2404399/90‑1 (MQ=255)

                                     tCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATc                                                <  2:2499815/90‑1 (MQ=255)

                                     tCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATc                                                <  1:2902445/90‑1 (MQ=255)

                                     tCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATc                                                <  2:2249817/90‑1 (MQ=255)

                                      cAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCg                                               <  1:3083528/90‑1 (MQ=255)

                                       aaCATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATTCAAAGCCCATCgc                                              >  2:8148/1‑89 (MQ=255)

                                       aaCATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGt                                              >  2:266115/1‑90 (MQ=255)

                                       aaCATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGt                                              <  1:1879824/90‑1 (MQ=255)

                                        aCATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGtt                                             >  1:1766768/1‑90 (MQ=255)

                                         cATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTg                                            <  1:133900/90‑1 (MQ=255)

                                         cATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTg                                            <  2:2573653/90‑1 (MQ=255)

                                          aTTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGa                                           <  1:1179140/90‑1 (MQ=255)

                                           ttACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaa                                          <  2:2870063/90‑1 (MQ=255)

                                            tACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaa                                         <  2:3155519/90‑1 (MQ=255)

                                             aCCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaa                                        <  2:2545533/90‑1 (MQ=255)

                                             aCCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaa                                        >  2:3217447/1‑90 (MQ=255)

                                              ccATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaaa                                       >  2:1176346/1‑90 (MQ=255)

                                              ccATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaaa                                       >  1:2327480/1‑90 (MQ=255)

                                                  tGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAAttt                                   >  2:1322368/1‑90 (MQ=255)

                                                   gCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAAtttt                                  <  2:1786024/90‑1 (MQ=255)

                                                    ccccATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTa                                 >  1:2371349/1‑90 (MQ=255)

                                                    ccccATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTa                                 >  2:593249/1‑90 (MQ=255)

                                                     cccATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTAt                                <  1:3108051/90‑1 (MQ=255)

                                                      ccATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATc                               <  1:1813518/90‑1 (MQ=255)

                                                       cATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATcc                              <  1:1422884/90‑1 (MQ=255)

                                                       cATTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATcc                              >  1:1041564/1‑90 (MQ=255)

                                                        aTTTAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCa                             <  2:2259517/90‑1 (MQ=255)

                                                          ttAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCata                           <  2:2896777/90‑1 (MQ=255)

                                                          ttAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCata                           <  2:2777036/90‑1 (MQ=255)

                                                          ttAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGGATACAAAGCCCATCGTTGAAAAAATTTTATCCata                           >  2:218754/1‑90 (MQ=255)

                                                           tAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCatat                          <  1:3360021/90‑1 (MQ=255)

                                                           tAAAGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCatat                          <  2:2536703/90‑1 (MQ=255)

                                                            aaaGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtt                         <  1:2647471/90‑1 (MQ=255)

                                                             aaGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAttt                        <  1:113229/90‑1 (MQ=255)

                                                             aaGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAttt                        <  1:3241274/90‑1 (MQ=255)

                                                             aaGCACAAAACCCGCTCATCAGCGGGCTTTCTACTTATTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAttt                        <  2:2313104/90‑1 (MQ=255)

                                                               gCACAAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAttttt                      <  2:1865968/90‑1 (MQ=255)

                                                                 acaAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTg                    <  2:605561/90‑1 (MQ=255)

                                                                 acaAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTg                    >  2:233996/1‑90 (MQ=255)

                                                                 acaAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTg                    >  2:2786464/1‑90 (MQ=255)

                                                                 acaAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGGATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTg                    >  2:2301653/1‑90 (MQ=255)

                                                                  caAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGa                   >  1:281324/1‑90 (MQ=255)

                                                                  caAAACCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGGTGAAAAAATTTTATCCATATTTTTTGa                   >  1:1619726/1‑90 (MQ=255)

                                                                   aaaaCCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGaa                  >  2:374964/1‑90 (MQ=255)

                                                                    aaaCCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGaaa                 <  1:1928418/90‑1 (MQ=255)

                                                                     aaCCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGGAAAAATTTTATCCATATTTTTTGaaaa                <  2:1810004/90‑1 (MQ=255)

                                                                     aaCCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGaaaa                >  2:1534689/1‑90 (MQ=255)

                                                                     aaCCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGaaaa                >  1:2581119/1‑90 (MQ=255)

                                                                      aCCCGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGaaaaa               >  2:1353714/1‑90 (MQ=255)

                                                                       cccGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAAt              >  1:769738/1‑90 (MQ=255)

                                                                        ccGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATg             >  2:1512691/1‑90 (MQ=255)

                                                                        ccGCTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATg             <  2:793382/90‑1 (MQ=255)

                                                                           cTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCaa          >  2:531112/1‑90 (MQ=255)

                                                                           cTCATCAGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCaa          >  1:2454538/1‑90 (MQ=255)

                                                                            tcatcaGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCaaa         >  2:32200/1‑90 (MQ=255)

                                                                            tcatcaGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCaaa         <  1:3338375/90‑1 (MQ=255)

                                                                              atcaGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAca       >  1:3022828/1‑90 (MQ=255)

                                                                                caGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatc     <  1:629077/90‑1 (MQ=255)

                                                                                 aGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatca    >  1:2759508/1‑90 (MQ=255)

                                                                                 aGCGGGCTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatca    <  1:2496867/90‑1 (MQ=255)

                                                                                  gCGGGCTTTCTAATTTTTCTTAACGTCGGGAATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatcat   >  2:1019534/1‑90 (MQ=255)

AATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATG  >  AC_000091/1644007‑1644179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`