BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	55,008	C→T	G701E (GGG→GAG)	imp ←	exported protein required for envelope biosynthesis and integrity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	55,008	0	C	T	100.0%	482.9 / NA	122	G701E (GGG→GAG)	imp	exported protein required for envelope biosynthesis and integrity
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (54/68); total (54/68)

Only 100 of 127 total aligned reads displayed.

ACGCGAATTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCCCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTCAGCAGTGGAA  >  AC_000091/54921‑55094

aCGCGAATTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTca                                                                                      <  2:551105/90‑1 (MQ=255)

aCGCGAATTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTca                                                                                      <  1:686984/90‑1 (MQ=255)

 cgcgAATTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTcaa                                                                                     >  2:2786083/1‑90 (MQ=255)

 cgcgAATTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTcaa                                                                                     >  2:624229/1‑90 (MQ=255)

   cgAATTGCATAGCAGCAGGAGCTGTATTTCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTcaaca                                                                                   <  1:1409825/90‑1 (MQ=255)

    gAATTGCATAGGAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCCTGTTAGCATTGGTGTTGTAATAGGAGGCCTcaacaa                                                                                  >  1:1288320/1‑90 (MQ=255)

    gAATTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTcaacaa                                                                                  >  1:1816176/1‑90 (MQ=255)

    gAATTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTcaacaa                                                                                  >  1:341369/1‑90 (MQ=255)

     aaTTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAAt                                                                                 <  1:1987842/90‑1 (MQ=255)

     aaTTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAAt                                                                                 >  2:2309380/1‑90 (MQ=255)

     aaTTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAAt                                                                                 >  2:529834/1‑90 (MQ=255)

      aTTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATg                                                                                >  1:1044649/1‑90 (MQ=255)

        tGCATAGCAGCAGGAGCTGCATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGa                                                                              <  1:574808/90‑1 (MQ=255)

           aTAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCa                                                                           <  2:2714770/90‑1 (MQ=255)

           aTAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCa                                                                           <  2:1935277/90‑1 (MQ=255)

            tAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCaa                                                                          >  2:3220921/1‑90 (MQ=255)

             agcagcAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAAc                                                                         <  2:1503504/90‑1 (MQ=255)

              gcagcaGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACg                                                                        <  1:387872/90‑1 (MQ=255)

                agcagGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGAt                                                                      <  1:440619/90‑1 (MQ=255)

                agcagGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGAt                                                                      >  2:1926775/1‑90 (MQ=255)

                 gcagGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATc                                                                     <  2:908582/90‑1 (MQ=255)

                  cagGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTATTAGTAGGCCTCAACAATCGACCAACGATCg                                                                    <  2:1616565/90‑1 (MQ=255)

                  cagGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCg                                                                    <  1:2350479/90‑1 (MQ=255)

                  cagGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCg                                                                    <  1:348769/90‑1 (MQ=255)

                     gAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGAGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCa                                                                 >  2:1100558/1‑90 (MQ=255)

                      aGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTATGCCTCAACAATGGACCAACGATCGGCaa                                                                >  1:1029158/1‑90 (MQ=255)

                       gCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAAt                                                               >  1:770620/1‑90 (MQ=255)

                         tGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTg                                                             >  1:212365/1‑90 (MQ=255)

                         tGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTAAACAATGGACCAACGATCGGCAATTg                                                             <  1:1456506/90‑1 (MQ=255)

                          gTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTgg                                                            >  1:1568174/1‑90 (MQ=255)

                           tATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGc                                                           >  2:3274517/1‑90 (MQ=255)

                           tATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGc                                                           >  1:2812233/1‑90 (MQ=255)

                            aTTGCACCCCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGcc                                                          <  1:2321845/90‑1 (MQ=255)

                            aTTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGcc                                                          <  2:1166588/90‑1 (MQ=255)

                            aTTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGcc                                                          <  1:2494442/90‑1 (MQ=255)

                            aTTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGcc                                                          >  1:3124034/1‑90 (MQ=255)

                            aTTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGcc                                                          >  2:2012541/1‑90 (MQ=255)

                            aTTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGcc                                                          <  1:1926248/90‑1 (MQ=255)

                            aTTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAATAGTAGGCCTCAACAATGGACCAACGATCGGCAAtttgac                                                          >  2:2237125/1‑86 (MQ=255)

                               gCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGc                                                       <  2:39422/90‑1 (MQ=255)

                                cacaCCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCt                                                      <  1:1114880/90‑1 (MQ=255)

                                cacaCCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCt                                                      >  1:1312573/1‑90 (MQ=255)

                                 acacCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTg                                                     <  1:893378/90‑1 (MQ=255)

                                 acacCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTg                                                     <  2:3247464/90‑1 (MQ=255)

                                  cacCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTgg                                                    >  2:1915956/1‑90 (MQ=255)

                                    ccTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCg                                                  <  1:3133346/90‑1 (MQ=255)

                                    ccTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCg                                                  <  1:907338/90‑1 (MQ=255)

                                     cTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGa                                                 >  1:835057/1‑90 (MQ=255)

                                     cTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGa                                                 <  1:2773408/90‑1 (MQ=255)

                                      tAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGAc                                                <  1:853584/90‑1 (MQ=255)

                                        aCATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAg                                              <  1:806518/90‑1 (MQ=255)

                                        aCATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAg                                              <  2:2016464/90‑1 (MQ=255)

                                         cATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGc                                             >  1:160972/1‑90 (MQ=255)

                                          aTAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCa                                            >  2:2773140/1‑90 (MQ=255)

                                           tAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGTCCAACGATCGGCAATTGGCCAGCTGGCGACAGCac                                           <  2:1252310/90‑1 (MQ=255)

                                            agagTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCacc                                          >  2:1553142/1‑90 (MQ=255)

                                             gagTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCacct                                         >  2:287108/1‑90 (MQ=255)

                                             gagTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCacct                                         <  2:2440322/90‑1 (MQ=255)

                                             gagTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCacct                                         <  2:2241191/90‑1 (MQ=255)

                                             gagTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCacct                                         <  2:1558843/90‑1 (MQ=255)

                                              agTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCaccta                                        >  1:702239/1‑90 (MQ=255)

                                                 cGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCacctacct                                     <  1:340265/90‑1 (MQ=255)

                                                   gcttgcttGTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGc                                   >  1:2145613/1‑90 (MQ=255)

                                                    cttgcttgTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCg                                  >  1:2323200/1‑90 (MQ=255)

                                                    cttgcttgTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCg                                  <  2:116450/90‑1 (MQ=255)

                                                    cttgcttgTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCg                                  <  1:1175056/90‑1 (MQ=255)

                                                     ttgcttgTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGa                                 >  2:1597692/1‑90 (MQ=255)

                                                     ttgcttgTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGa                                 >  2:2493286/1‑90 (MQ=255)

                                                       gcttgTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGaaa                               <  1:3183158/90‑1 (MQ=255)

                                                        cttgTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAAt                              >  1:2502838/1‑90 (MQ=255)

                                                          tgTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATAc                            >  1:3455250/1‑90 (MQ=255)

                                                          tgTTAGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATAc                            <  1:337287/90‑1 (MQ=255)

                                                              aGCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCAtt                        <  1:1835792/90‑1 (MQ=255)

                                                               gCATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTc                       <  1:2794458/90‑1 (MQ=255)

                                                                cATTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCt                      >  1:2955110/1‑90 (MQ=255)

                                                                 aTTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCtt                     <  2:2202789/90‑1 (MQ=255)

                                                                 aTTGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCtt                     <  1:1069639/90‑1 (MQ=255)

                                                                  ttGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTTCCTGCGAAATACCATTCTta                    <  2:688365/90‑1 (MQ=255)

                                                                  ttGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTta                    >  2:2608025/1‑90 (MQ=255)

                                                                  ttGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTta                    <  2:920327/90‑1 (MQ=255)

                                                                  ttGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTta                    <  1:3113346/90‑1 (MQ=255)

                                                                  ttGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCAGGCGACAGCACCTACCTGCGAAATACCATTCTta                    <  2:1686097/90‑1 (MQ=255)

                                                                   tGGTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTtat                   <  1:927759/90‑1 (MQ=255)

                                                                    ggTGTCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTtata                  <  2:2908410/90‑1 (MQ=255)

                                                                      tgtCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATAtt                <  2:1873730/90‑1 (MQ=255)

                                                                      tgtCGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATAtt                <  2:1932945/90‑1 (MQ=255)

                                                                         cGTAGTAGTAGGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCt             <  1:2672448/90‑1 (MQ=255)

                                                                            agtagtagGTCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTcag          >  2:33545/1‑90 (MQ=255)

                                                                             gtagtagGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTcagc         <  2:2074279/90‑1 (MQ=255)

                                                                              tagttgGCCTCAACAATGGTCCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATTCCATTCTTATATTGCTcagca        <  2:2872094/90‑1 (MQ=255)

                                                                               agtagGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTcagcag       <  1:1828932/90‑1 (MQ=255)

                                                                               agtagGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTcagcag       >  1:1189678/1‑90 (MQ=255)

                                                                               agtagGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTcagcag       >  1:1739494/1‑90 (MQ=255)

                                                                               agtagGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTcagcag       >  1:1873466/1‑90 (MQ=255)

                                                                                 tagGCCTCAACAGTGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTCAGCAGTg     >  2:235101/1‑90 (MQ=255)

                                                                                 tagGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTCAGCAGTg     >  2:2463537/1‑90 (MQ=255)

                                                                                  agGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTCAGCAGTgg    >  1:1479925/1‑90 (MQ=255)

                                                                                  agGCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTCAGCAGTgg    <  1:2546110/90‑1 (MQ=255)

                                                                                    gCCTCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTCAGCAGTGGaa  <  1:1000048/90‑1 (MQ=255)

                                                                                    gCCACAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTCAGCAGTGGaa  <  1:3477724/90‑1 (MQ=255)

ACGCGAATTGCATAGCAGCAGGAGCTGTATTGCACACCTAACATAGAGTCGGCTTGCTTGTTAGCATTGGTGTCGTAGTAGTAGGCCCCAACAATGGACCAACGATCGGCAATTGGCCAGCTGGCGACAGCACCTACCTGCGAAATACCATTCTTATATTGCTCAGCAGTGGAA  >  AC_000091/54921‑55094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`