BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,873,445	C→T	T449T (ACC→ACT)	yeaI →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,873,445	0	C	T	100.0%	537.3 / NA	133	T449T (ACC→ACT)	yeaI	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (77/56); total (77/56)

Only 100 of 133 total aligned reads displayed.

GGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGC  >  AC_000091/1873358‑1873531

ggCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTAt                                                                                      <  1:389164/90‑1 (MQ=255)

ggCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTAt                                                                                      <  1:1872692/90‑1 (MQ=255)

 gCAAAAATCATTGGGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATc                                                                                     <  1:2752510/90‑1 (MQ=255)

 gCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATc                                                                                     >  2:467676/1‑90 (MQ=255)

  cAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCa                                                                                    >  1:672776/1‑90 (MQ=255)

     aaaTCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTa                                                                                 >  1:1109793/1‑90 (MQ=255)

      aaTCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTAt                                                                                >  2:2608568/1‑90 (MQ=255)

       aTCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTAtt                                                                               <  2:3481601/90‑1 (MQ=255)

        tCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTg                                                                              >  2:675161/1‑90 (MQ=255)

         cATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTgg                                                                             <  2:3083533/90‑1 (MQ=255)

         cATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTgg                                                                             >  1:3141148/1‑90 (MQ=255)

         cATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTgg                                                                             <  1:171365/90‑1 (MQ=255)

         cATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTgg                                                                             <  1:2234663/90‑1 (MQ=255)

          aTTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGc                                                                            >  2:706848/1‑90 (MQ=255)

           ttGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCa                                                                           <  1:1590862/90‑1 (MQ=255)

           ttGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCa                                                                           <  1:2171277/90‑1 (MQ=255)

              cGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACgg                                                                        >  1:234123/1‑90 (MQ=255)

                 aaCGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAGCAAATGACTATCAGTATTGGCACGGttt                                                                     >  2:844468/1‑90 (MQ=255)

                 aaCGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGttt                                                                     >  1:2958706/1‑90 (MQ=255)

                 aaCGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGttt                                                                     <  2:2377736/90‑1 (MQ=255)

                  aCGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGtttt                                                                    >  1:1113628/1‑90 (MQ=255)

                   cGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGttttt                                                                   <  2:2507775/90‑1 (MQ=255)

                   cGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGttttt                                                                   <  1:1110897/90‑1 (MQ=255)

                   cGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGttttt                                                                   <  2:2493966/90‑1 (MQ=255)

                    gTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGtttttt                                                                  <  2:1126328/90‑1 (MQ=255)

                    gTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGtttttt                                                                  >  2:1183177/1‑90 (MQ=255)

                    gTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGtttttt                                                                  >  1:828026/1‑90 (MQ=255)

                      aTGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCa                                                                >  1:565284/1‑90 (MQ=255)

                      aTGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCa                                                                <  2:2000603/90‑1 (MQ=255)

                        gCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAAc                                                              <  2:324965/90‑1 (MQ=255)

                         cGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACg                                                             <  2:2020558/90‑1 (MQ=255)

                         cGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACg                                                             >  2:2935697/1‑90 (MQ=255)

                            aaaaaTGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGt                                                          >  1:1684563/1‑90 (MQ=255)

                            aaaaaTGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGt                                                          >  2:1650141/1‑90 (MQ=255)

                            aaaaaTGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGt                                                          >  1:1628674/1‑90 (MQ=255)

                             aaaaTGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTg                                                         >  2:2684819/1‑90 (MQ=255)

                              aaaTGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGa                                                        <  1:481923/90‑1 (MQ=255)

                              aaaTGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGa                                                        >  2:3037370/1‑90 (MQ=255)

                               aaTGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGac                                                       <  1:50222/90‑1 (MQ=255)

                                 tGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGacac                                                     <  1:1526415/90‑1 (MQ=255)

                                  gTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACAcg                                                    <  2:2466625/90‑1 (MQ=255)

                                     gAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGt                                                 >  1:697029/1‑90 (MQ=255)

                                     gAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGt                                                 >  1:302607/1‑90 (MQ=255)

                                       aCTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTaa                                               >  2:1006879/1‑90 (MQ=255)

                                        cTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAat                                              >  2:739844/1‑90 (MQ=255)

                                        cTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAat                                              >  2:1063910/1‑90 (MQ=255)

                                         tCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAata                                             >  1:2675966/1‑90 (MQ=255)

                                          ccTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAatat                                            <  1:1510371/90‑1 (MQ=255)

                                            tGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATAtct                                          >  2:2321682/1‑90 (MQ=255)

                                            tGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATAtct                                          >  1:985084/1‑90 (MQ=255)

                                            tGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATAtct                                          >  1:593428/1‑90 (MQ=255)

                                            tGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATAtct                                          >  2:2103690/1‑90 (MQ=255)

                                             gACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATAtctc                                         <  2:57093/90‑1 (MQ=255)

                                               ccGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGc                                       <  2:2382337/90‑1 (MQ=255)

                                                   cTTTAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGt                                   <  2:2470066/90‑1 (MQ=255)

                                                     ttAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCa                                 >  2:3484969/1‑90 (MQ=255)

                                                     ttAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCa                                 >  1:1907277/1‑90 (MQ=255)

                                                      tAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCAt                                >  2:415881/1‑90 (MQ=255)

                                                      tAGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCAt                                <  1:1248220/90‑1 (MQ=255)

                                                       aGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATg                               <  1:1549652/90‑1 (MQ=255)

                                                       aGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATg                               <  2:3336890/90‑1 (MQ=255)

                                                       aGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATg                               <  2:101375/90‑1 (MQ=255)

                                                       aGTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATg                               >  1:31533/1‑90 (MQ=255)

                                                        gTAACAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGa                              <  2:2157424/90‑1 (MQ=255)

                                                          aaCAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACg                            >  1:1755504/1‑90 (MQ=255)

                                                           aCAGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACgg                           <  1:2970581/90‑1 (MQ=255)

                                                             aGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCCCGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGaa                         >  2:2568740/1‑90 (MQ=255)

                                                             aGATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGaa                         >  1:1205470/1‑90 (MQ=255)

                                                              gATATGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAg                        <  2:1921476/90‑1 (MQ=255)

                                                               atatGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGc                       <  2:191597/90‑1 (MQ=255)

                                                               atatGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGc                       >  2:2463949/1‑90 (MQ=255)

                                                               atatGATGTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGc                       <  2:736735/90‑1 (MQ=255)

                                                                 atgatgTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAg                     <  2:1700823/90‑1 (MQ=255)

                                                                  tgatgTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGa                    <  2:2027159/90‑1 (MQ=255)

                                                                  tgatgTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGa                    >  1:2649049/1‑90 (MQ=255)

                                                                   gatgTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGAt                   <  2:1894753/90‑1 (MQ=255)

                                                                   gatgTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGAt                   >  2:2621071/1‑90 (MQ=255)

                                                                    atgTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATa                  <  1:2723604/90‑1 (MQ=255)

                                                                     tgTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATaa                 >  2:635658/1‑90 (MQ=255)

                                                                      gTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATTTCGCTTGTCATGACGGAAGCAGATaaa                >  2:2869156/1‑90 (MQ=255)

                                                                      gTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATaaa                <  2:1542458/90‑1 (MQ=255)

                                                                      gTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATaaa                >  2:1082311/1‑90 (MQ=255)

                                                                      gTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATaaa                >  2:860165/1‑90 (MQ=255)

                                                                      gTTCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATaaa                >  1:2093697/1‑90 (MQ=255)

                                                                       ttCCTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAg               >  2:1427413/1‑90 (MQ=255)

                                                                          cTGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCt            <  2:3442043/90‑1 (MQ=255)

                                                                           tGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACTGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCtt           <  2:317673/90‑1 (MQ=255)

                                                                           tGAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCtt           >  2:1652505/1‑90 (MQ=255)

                                                                            gAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGTTTGTCATGACGGAAGCAGATAAAGCCTTa          <  2:880456/90‑1 (MQ=255)

                                                                            gAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTa          <  2:2117068/90‑1 (MQ=255)

                                                                            gAACAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCATTa          >  2:3026284/1‑90 (MQ=255)

                                                                             aaCAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTAc         <  1:167425/90‑1 (MQ=255)

                                                                              aCAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTAcg        <  1:1220598/90‑1 (MQ=255)

                                                                               cAAATGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTAcgc       >  1:2943330/1‑90 (MQ=255)

                                                                                  aTGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACgcgaa    >  1:2114710/1‑90 (MQ=255)

                                                                                  aTGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTCGTCATGACGGAAGCAGATAAAGCCTTACgcgaa    >  2:399743/1‑90 (MQ=255)

                                                                                   tGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACgcgaag   >  1:1049071/1‑90 (MQ=255)

                                                                                   tGACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACgcgaag   >  1:3171309/1‑90 (MQ=255)

                                                                                    gACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACgcgaagc  >  2:285136/1‑90 (MQ=255)

                                                                                    gACTATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACgcgaagc  <  1:360686/90‑1 (MQ=255)

GGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGC  >  AC_000091/1873358‑1873531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`