BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	2,153,956	T→C	E218E (GAA→GAG)	yegK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	2,153,956	0	T	C	100.0%	455.1 / NA	118	E218E (GAA→GAG)	yegK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (64/54); total (64/54)

Only 100 of 124 total aligned reads displayed.

TGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCG  >  AC_000091/2153869‑2154042

tGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTc                                                                                      <  2:2904324/90‑1 (MQ=255)

tGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTc                                                                                      >  1:1656537/1‑90 (MQ=255)

 gCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTcc                                                                                     <  2:2556159/90‑1 (MQ=255)

 gCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTcc                                                                                     >  1:3144085/1‑90 (MQ=255)

 gCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTcc                                                                                     >  2:2229508/1‑90 (MQ=255)

  ccAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCt                                                                                    <  2:2746929/90‑1 (MQ=255)

  ccAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCt                                                                                    <  1:1336828/90‑1 (MQ=255)

   cAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTg                                                                                   >  1:2509416/1‑90 (MQ=255)

    aaCGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGc                                                                                  >  2:2755078/1‑90 (MQ=255)

     aCGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCg                                                                                 >  1:1702301/1‑90 (MQ=255)

     aCGTTTTATCATCATCCGTACGCTCGGTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCg                                                                                 >  1:2047854/1‑90 (MQ=255)

      cGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGt                                                                                >  1:2737649/1‑90 (MQ=255)

       attttATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGtt                                                                               <  2:3353996/89‑1 (MQ=255)

         tttATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTtgc                                                                             >  1:286029/1‑90 (MQ=255)

          ttATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTtgct                                                                            <  2:2381332/90‑1 (MQ=255)

           tATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTtgctg                                                                           <  2:2320146/90‑1 (MQ=255)

             tcatcatcCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTCAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTgcc                                                                         <  2:2779113/90‑1 (MQ=255)

                 catcCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCa                                                                     <  1:2382872/90‑1 (MQ=255)

                   tcCGTACGCTCGTTTCCCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGt                                                                   <  2:725705/90‑1 (MQ=255)

                    ccGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTc                                                                  >  1:1828048/1‑90 (MQ=255)

                    ccGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTc                                                                  <  1:2129878/90‑1 (MQ=255)

                     cGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTcc                                                                 <  2:1106810/90‑1 (MQ=255)

                     cGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTcc                                                                 >  1:967506/1‑90 (MQ=255)

                      gTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCa                                                                >  2:1758090/1‑90 (MQ=255)

                      gTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCa                                                                <  1:1195409/90‑1 (MQ=255)

                       tACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCAt                                                               <  2:2277747/90‑1 (MQ=255)

                       tACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCAt                                                               <  2:2016433/90‑1 (MQ=255)

                       tACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCAt                                                               <  2:2253182/90‑1 (MQ=255)

                       tACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCAt                                                               >  2:3315684/1‑90 (MQ=255)

                          gCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGa                                                            >  1:2603635/1‑90 (MQ=255)

                           cTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGaa                                                           <  1:540628/90‑1 (MQ=255)

                               tttACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAAc                                                       <  2:2091441/90‑1 (MQ=255)

                                ttACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACg                                                      >  1:3430389/1‑90 (MQ=255)

                                ttACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACg                                                      >  2:2869682/1‑90 (MQ=255)

                                ttACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTCCGTTGCTGCCGCCAGTCCATTGAAAAACg                                                      <  2:120010/90‑1 (MQ=255)

                                  aCCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACggg                                                    <  2:1169404/90‑1 (MQ=255)

                                  aCCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACggg                                                    <  2:2986348/90‑1 (MQ=255)

                                  aCCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACggg                                                    <  2:1454671/90‑1 (MQ=255)

                                   ccgccgGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACgggg                                                   <  1:1631691/90‑1 (MQ=255)

                                   ccgccgGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACgggg                                                   <  1:870784/90‑1 (MQ=255)

                                    cgccgGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGt                                                  <  1:3099951/90‑1 (MQ=255)

                                    cgccgGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGt                                                  <  1:851270/90‑1 (MQ=255)

                                    cgccgGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGt                                                  <  1:241308/90‑1 (MQ=255)

                                       cgGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaa                                               >  1:1911295/1‑90 (MQ=255)

                                           cTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaa                                           <  1:729504/90‑1 (MQ=255)

                                           cTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaa                                           >  1:1412903/1‑90 (MQ=255)

                                           cTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaa                                           >  1:2266732/1‑90 (MQ=255)

                                            tGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaag                                          <  1:831815/90‑1 (MQ=255)

                                             ggACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAgg                                         >  1:3134091/1‑90 (MQ=255)

                                              gACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGc                                        >  1:3314957/1‑90 (MQ=255)

                                               aCAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGca                                       >  2:724250/1‑90 (MQ=255)

                                                cAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcac                                      <  2:3018708/90‑1 (MQ=255)

                                                 aaaaaCTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcaca                                     >  2:2585744/1‑90 (MQ=255)

                                                   aaaCTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATg                                   >  1:1029114/1‑90 (MQ=255)

                                                   aaaCTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATg                                   <  2:2046140/90‑1 (MQ=255)

                                                   aaaCTGCTTTAAAAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATg                                   <  2:2539846/90‑1 (MQ=255)

                                                    aaCTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATga                                  >  1:260182/1‑90 (MQ=255)

                                                    aaCTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATga                                  >  1:2226697/1‑90 (MQ=255)

                                                    aaCTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATga                                  <  2:2651886/90‑1 (MQ=255)

                                                    aaCTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATga                                  >  1:625429/1‑90 (MQ=255)

                                                    aaCTGCTTTAACAATTCAGGTAATAAATCGAGTTGCCCCTGCGTTGCTGCCGCCAGTCCATTGAAAATAGGGGTAAAGAAAGGCACATga                                  >  2:1955220/1‑90 (MQ=255)

                                                     aCTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                 >  1:3347905/1‑90 (MQ=255)

                                                      cTGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagg                                <  1:2079203/90‑1 (MQ=255)

                                                       tGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagga                               >  2:2958299/1‑90 (MQ=255)

                                                       tGCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagga                               >  1:2391065/1‑90 (MQ=255)

                                                        gCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgaggag                              >  1:809617/1‑90 (MQ=255)

                                                        gCTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgaggag                              <  2:2508438/90‑1 (MQ=255)

                                                         cTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGgaga                             >  2:1803847/1‑90 (MQ=255)

                                                         cTTTAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGgaga                             >  1:3165050/1‑90 (MQ=255)

                                                          tttAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGaa                            <  2:2483244/90‑1 (MQ=255)

                                                          tttAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGaa                            >  2:447090/1‑90 (MQ=255)

                                                           ttAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAAt                           >  1:1133987/1‑90 (MQ=255)

                                                            tAACAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAAtt                          >  1:1103848/1‑90 (MQ=255)

                                                             aaCAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTa                         >  1:1911558/1‑90 (MQ=255)

                                                              aCAATTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTAt                        <  2:2346661/90‑1 (MQ=255)

                                                                aaTTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGTAGAATTATcc                      >  1:2494055/1‑90 (MQ=255)

                                                                aaTTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATcc                      <  2:1721574/90‑1 (MQ=255)

                                                                aaTTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATcc                      >  2:1428276/1‑90 (MQ=255)

                                                                aaTTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATcc                      >  2:3006430/1‑90 (MQ=255)

                                                                aaTTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATcc                      >  1:2086858/1‑90 (MQ=255)

                                                                aaTTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATcc                      >  1:3277248/1‑90 (MQ=255)

                                                                 aTTCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCa                     <  1:2202527/90‑1 (MQ=255)

                                                                  ttCAGGTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAg                    >  1:885656/1‑90 (MQ=255)

                                                                      ggTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCata                <  1:2118100/90‑1 (MQ=255)

                                                                       gTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCatat               <  1:2437273/90‑1 (MQ=255)

                                                                       gTAATAAATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCatat               >  1:55832/1‑90 (MQ=255)

                                                                         aataaATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAttt             <  2:386859/90‑1 (MQ=255)

                                                                         aataaATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAttt             >  1:1589370/1‑90 (MQ=255)

                                                                         aataaATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAttt             <  1:28427/90‑1 (MQ=255)

                                                                         aataaATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAttt             >  1:2840680/1‑90 (MQ=255)

                                                                         aataaATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAttt             >  2:3190474/1‑90 (MQ=255)

                                                                          ataaATCGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTa            >  1:1078349/1‑90 (MQ=255)

                                                                             aaTCGAGTTGCTCCTGCGTTGCTGCCGCCCGTCCATTTAAAAACGGGGGAAAGAAAAGCACATGAGGAGAATTATCCAGCATATTTAACg         >  2:2873346/1‑90 (MQ=255)

                                                                                cGAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCa      <  1:3331669/90‑1 (MQ=255)

                                                                                 gAGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg     <  1:2347541/90‑1 (MQ=255)

                                                                                 gAGTTGCTCCAGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGCAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg     <  1:140681/90‑1 (MQ=255)

                                                                                  aGTTGCTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGt    <  2:3411760/90‑1 (MQ=255)

                                                                                   gttgctCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTc   >  1:1150337/1‑90 (MQ=255)

                                                                                    ttgctCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCg  <  2:342853/90‑1 (MQ=255)

                                                                                    ttgctCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCg  >  1:690269/1‑90 (MQ=255)

TGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCG  >  AC_000091/2153869‑2154042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`