BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	2,248,614	G→A	A497V (GCA→GTA)	cirA ←	ferric iron‑catecholate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	2,248,614	0	G	A	100.0%	516.0 / NA	133	A497V (GCA→GTA)	cirA	ferric iron‑catecholate outer membrane transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (62/71); total (62/71)

Only 100 of 135 total aligned reads displayed.

GCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCAACCCCTTCC  >  AC_000091/2248527‑2248700

  tttGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGc                                                                                    >  1:523533/1‑90 (MQ=255)

  tttGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGc                                                                                    >  2:866033/1‑90 (MQ=255)

  tttGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGc                                                                                    >  1:277141/1‑90 (MQ=255)

  tttGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGc                                                                                    <  2:1461119/90‑1 (MQ=255)

   ttGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCg                                                                                   >  2:2079216/1‑90 (MQ=255)

     gTTAACGTTGTAGTAGCTAAATCCCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGtt                                                                                 <  2:2169657/90‑1 (MQ=255)

     gTTAACGTTGTAGTAGCTAAATACCGGTATGCGTCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGtt                                                                                 >  2:1271679/1‑90 (MQ=255)

     gTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGtt                                                                                 <  1:1396887/90‑1 (MQ=255)

       tAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGa                                                                               >  2:3298321/1‑90 (MQ=255)

        aaCGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGAc                                                                              <  2:188229/90‑1 (MQ=255)

        aaCGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGAc                                                                              >  1:343820/1‑90 (MQ=255)

        aaCGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGAc                                                                              >  2:2484226/1‑90 (MQ=255)

         aCGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACg                                                                             <  1:1885510/90‑1 (MQ=255)

         aCGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACg                                                                             <  1:135044/90‑1 (MQ=255)

           gTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTc                                                                           <  1:2418952/90‑1 (MQ=255)

            ttGGAGTAGCTAAATGCCGGTATGGGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCa                                                                          <  2:14233/90‑1 (MQ=255)

             tGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAg                                                                         >  1:287634/1‑90 (MQ=255)

             tGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGAGTTGACGTCAg                                                                         >  2:2409950/1‑90 (MQ=255)

               tagtagCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAANNAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGAc                                                                       >  2:2710281/1‑90 (MQ=255)

               tagtagCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGAc                                                                       >  1:1029983/1‑90 (MQ=255)

               tagtagCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGAc                                                                       <  2:1367764/90‑1 (MQ=255)

                agtagCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACg                                                                      >  1:2239383/1‑90 (MQ=255)

                agtagCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACg                                                                      >  1:2915624/1‑90 (MQ=255)

                  tagCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTa                                                                    <  1:446241/90‑1 (MQ=255)

                  tagCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTa                                                                    <  1:3310394/90‑1 (MQ=255)

                   agCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTAc                                                                   <  1:1565156/90‑1 (MQ=255)

                    gCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACg                                                                  >  1:524005/1‑90 (MQ=255)

                    gCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACg                                                                  <  1:2653608/90‑1 (MQ=255)

                      tAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGgc                                                                >  2:2510587/1‑90 (MQ=255)

                      tAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGgc                                                                >  2:830918/1‑90 (MQ=255)

                        aaTACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGgctg                                                              >  2:3445507/1‑90 (MQ=255)

                          tACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGAACGgctgat                                                            >  2:2204301/1‑90 (MQ=255)

                           aCCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGgctgatg                                                           <  1:2598983/90‑1 (MQ=255)

                           aCCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGgctgatg                                                           >  1:2228438/1‑90 (MQ=255)

                             cGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGgctgatgct                                                         <  1:3047092/90‑1 (MQ=255)

                             cGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGgctgatgct                                                         >  2:1034058/1‑90 (MQ=255)

                              ggTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGgctgatgctg                                                        >  1:434656/1‑90 (MQ=255)

                               gTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGAAGCCCGGTACAGCGTTGACGTCAGACGTACGgctgatgctga                                                       >  1:145406/1‑90 (MQ=255)

                                tATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGgctgatgctgat                                                      <  2:3072864/90‑1 (MQ=255)

                                 aTGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATa                                                     >  1:1983515/1‑90 (MQ=255)

                                 aTGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATa                                                     <  1:3077013/90‑1 (MQ=255)

                                 aTGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATa                                                     >  2:2523726/1‑90 (MQ=255)

                                 aTGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATa                                                     >  2:635665/1‑90 (MQ=255)

                                  tGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATAc                                                    >  1:2691554/1‑90 (MQ=255)

                                   gcgcCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACg                                                   <  1:105155/90‑1 (MQ=255)

                                    cgcCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGa                                                  <  2:3323169/90‑1 (MQ=255)

                                     gcCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGAt                                                 <  2:2836182/90‑1 (MQ=255)

                                     gcCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGAt                                                 >  2:175283/1‑90 (MQ=255)

                                      ccgtccgtTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATc                                                <  1:3336023/90‑1 (MQ=255)

                                      ccgtccgtTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATc                                                <  1:2890296/90‑1 (MQ=255)

                                       cgtccgtTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCt                                               <  1:553893/90‑1 (MQ=255)

                                       cgtccgtTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCt                                               <  2:3439977/90‑1 (MQ=255)

                                       cgtccgtTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCt                                               <  1:3411531/90‑1 (MQ=255)

                                        gtccgtTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCtt                                              <  2:163719/90‑1 (MQ=255)

                                        gtccgtTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCtt                                              >  1:1522192/1‑90 (MQ=255)

                                        gtccgtTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCtt                                              <  2:3378130/90‑1 (MQ=255)

                                          ccgtTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTc                                            <  2:2605993/90‑1 (MQ=255)

                                           cgtTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTAAGGCTGATGCTGATACGATCTTTca                                           >  1:2862594/1‑90 (MQ=255)

                                              tAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACAt                                        <  2:1518000/90‑1 (MQ=255)

                                              tAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACAt                                        <  1:446100/90‑1 (MQ=255)

                                              tAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACAt                                        <  2:2145632/90‑1 (MQ=255)

                                                   cccGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTa                                   <  2:2965293/90‑1 (MQ=255)

                                                    tcGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTACACATCGTTAc                                  <  1:555057/89‑1 (MQ=255)

                                                     cGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACg                                 >  1:3209816/1‑90 (MQ=255)

                                                         tcAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGaaa                             <  2:1098261/90‑1 (MQ=255)

                                                          cAAAACCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGaaaa                            >  1:2521636/1‑90 (MQ=255)

                                                           aaacCCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAAc                           >  2:1830378/1‑90 (MQ=255)

                                                           aaaaCCAACAAAGTTTTTGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAAc                           <  2:2870890/90‑1 (MQ=255)

                                                           aaaaCCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAAc                           >  2:909705/1‑90 (MQ=255)

                                                           aaaaCCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAAc                           <  2:2451877/90‑1 (MQ=255)

                                                            aaaCCAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACg                          >  2:171686/1‑90 (MQ=255)

                                                               ccAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTa                       >  1:1130008/1‑90 (MQ=255)

                                                               ccAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTa                       >  1:2035960/1‑90 (MQ=255)

                                                               ccAACAAAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTa                       <  2:3270115/90‑1 (MQ=255)

                                                                caacaaAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTaa                      <  2:2310267/90‑1 (MQ=255)

                                                                 aacaaAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAAc                     <  2:2904568/90‑1 (MQ=255)

                                                                  acaaAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACg                    <  1:61848/90‑1 (MQ=255)

                                                                   caaAGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGc                   <  2:1781419/90‑1 (MQ=255)

                                                                    aaaGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCt                  <  2:2718336/90‑1 (MQ=255)

                                                                    aaaGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCt                  >  2:776775/1‑90 (MQ=255)

                                                                    aaaGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCt                  <  2:828875/90‑1 (MQ=255)

                                                                    aaaGTTTTGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCt                  <  2:220747/90‑1 (MQ=255)

                                                                    aaaGTTTTGGTAGCCCGGTACAGCATTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCt                  <  1:1155677/90‑1 (MQ=255)

                                                                    aaaGTTTTGGGAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCt                  <  2:2779268/90‑1 (MQ=255)

                                                                        ttttGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGAt              >  1:1933469/1‑90 (MQ=255)

                                                                        ttttGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGAt              >  2:404407/1‑90 (MQ=255)

                                                                        ttttGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGAt              >  2:2682985/1‑90 (MQ=255)

                                                                         tttGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGAtt             <  1:2760187/90‑1 (MQ=255)

                                                                           tGGTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCa           <  1:2729301/90‑1 (MQ=255)

                                                                            ggTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCaa          >  1:9830/1‑90 (MQ=255)

                                                                            ggTAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCaa          >  2:397213/1‑90 (MQ=255)

                                                                              tAGCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCAAcc        >  1:52053/1‑90 (MQ=255)

                                                                                gCCCGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCAAcccc      <  1:2629486/90‑1 (MQ=255)

                                                                                 cccGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCAACCCCt     >  1:1394421/1‑90 (MQ=255)

                                                                                 cccGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCAACCCCt     <  1:2444857/90‑1 (MQ=255)

                                                                                 cccGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCAACCCCt     >  1:1601670/1‑90 (MQ=255)

                                                                                 cccGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCAACCCCt     <  1:2792449/90‑1 (MQ=255)

                                                                                  ccGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCAACCCCtt    <  2:3251356/90‑1 (MQ=255)

                                                                                   cGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCAACCCCTTc   >  2:3394403/1‑90 (MQ=255)

                                                                                   cGGTACAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCAACCCCTTc   <  1:1161318/90‑1 (MQ=255)

GCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTTTCACATCGTTACGGAAAACGGTAACGCTGGATTCAACCCCTTCC  >  AC_000091/2248527‑2248700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`