BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	2,265,849	T→C	K327K (AAA→AAG)	fruB ←	fused fructose‑specific PTS enzyme IIA component and HPr component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	2,265,849	0	T	C	100.0%	458.0 / NA	113	K327K (AAA→AAG)	fruB	fused fructose‑specific PTS enzyme IIA component and HPr component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (56/57); total (56/57)

Only 100 of 140 total aligned reads displayed.

ATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTGGACGAGCATG  >  AC_000091/2265762‑2265935

aTCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTCACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggctt                                                                                      <  2:433896/90‑1 (MQ=255)

aTCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggctt                                                                                      <  2:1877987/90‑1 (MQ=255)

aTCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggctt                                                                                      <  1:2398036/90‑1 (MQ=255)

aTCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggctt                                                                                      >  2:672800/1‑90 (MQ=255)

aTCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggctt                                                                                      <  1:940886/90‑1 (MQ=255)

 tCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggcttg                                                                                     <  1:1360920/90‑1 (MQ=255)

  cTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggcttgc                                                                                    <  1:1322461/90‑1 (MQ=255)

  cTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCAAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggcttgc                                                                                    >  2:1784314/1‑90 (MQ=255)

   tGCACCCTGGGCGGAAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggcttgcc                                                                                   <  1:994505/90‑1 (MQ=255)

    gCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggcttgccg                                                                                  >  1:1895236/1‑90 (MQ=255)

     cACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGtttgccggcttgccgg                                                                                 <  1:3446767/90‑1 (MQ=255)

      aCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTttgccggcttgccggt                                                                                >  1:320424/1‑90 (MQ=255)

      aCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTttgccggcttgccggt                                                                                >  1:1119150/1‑90 (MQ=255)

        ccTGGGCGCTAAAGCGTAGGCGATGACCTTTCTCAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCTGTAc                                                                              <  2:829175/90‑1 (MQ=255)

         cTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTAcc                                                                             <  1:593912/90‑1 (MQ=255)

          tGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCa                                                                            >  1:1998845/1‑90 (MQ=255)

           gggCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCAt                                                                           <  1:2187735/90‑1 (MQ=255)

              cGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCaa                                                                        >  2:1183062/1‑90 (MQ=255)

               ggTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAg                                                                       >  2:3268393/1‑90 (MQ=255)

                 tAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTTTCGGTACCATCAAgga                                                                     >  2:814349/1‑89 (MQ=255)

                  aaaGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAgggt                                                                    >  2:3198144/1‑88 (MQ=255)

                  aaaGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGtt                                                                    <  2:350730/90‑1 (MQ=255)

                  aaaGCGTAGGCGATGACCTTTCTGAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGtt                                                                    >  2:62701/1‑90 (MQ=255)

                    aGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTg                                                                  <  2:3448177/90‑1 (MQ=255)

                     gCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGt                                                                 <  2:3219942/90‑1 (MQ=255)

                     gCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGt                                                                 <  2:2550537/90‑1 (MQ=255)

                        tAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCAc                                                              >  1:945526/1‑90 (MQ=255)

                        tAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCAc                                                              >  2:3312405/1‑90 (MQ=255)

                          ggCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACgg                                                            >  2:594115/1‑90 (MQ=255)

                           gCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGt                                                           >  1:1217735/1‑90 (MQ=255)

                             gATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTaa                                                         >  1:1729293/1‑90 (MQ=255)

                              aTGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAat                                                        >  1:1040874/1‑90 (MQ=255)

                                gACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAatat                                                      >  2:2431107/1‑90 (MQ=255)

                                gACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAatat                                                      <  1:1734766/90‑1 (MQ=255)

                                gACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGGTTGTCACGGGAatat                                                      >  1:2323858/1‑90 (MQ=255)

                                 cccTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATc                                                     <  2:2174062/89‑1 (MQ=255)

                                 aCCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATc                                                     <  2:988780/90‑1 (MQ=255)

                                 aCCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATc                                                     >  1:1842330/1‑90 (MQ=255)

                                  ccTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGTTACCATCAAGGTTTGTCACGGTAAAATct                                                    >  1:147256/1‑89 (MQ=255)

                                  ccTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCa                                                    >  1:2879295/1‑90 (MQ=255)

                                  ccTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCa                                                    >  2:2834046/1‑90 (MQ=255)

                                   cTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCAc                                                   <  2:507392/90‑1 (MQ=255)

                                     ttCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTg                                                 >  2:2528167/1‑90 (MQ=255)

                                     ttCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTg                                                 >  2:584228/1‑90 (MQ=255)

                                     ttCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTg                                                 <  1:1411094/90‑1 (MQ=255)

                                      tCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGt                                                >  2:2345051/1‑90 (MQ=255)

                                      tCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGt                                                >  2:257583/1‑90 (MQ=255)

                                       cTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGtt                                               <  2:3089105/90‑1 (MQ=255)

                                       cTTAACACCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGtt                                               <  2:1808814/90‑1 (MQ=255)

                                        ttAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTa                                              <  1:3212390/90‑1 (MQ=255)

                                        ttAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTa                                              <  1:448094/90‑1 (MQ=255)

                                        ttAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTa                                              <  2:308637/90‑1 (MQ=255)

                                         tAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTaa                                             >  2:2105537/1‑90 (MQ=255)

                                           aCGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAAt                                           <  1:163024/90‑1 (MQ=255)

                                           aCGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAAt                                           >  1:3448125/1‑90 (MQ=255)

                                            cGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAAtt                                          >  2:799086/1‑90 (MQ=255)

                                            cGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGGAATATCACTGTTAAAtt                                          >  1:2556692/1‑90 (MQ=255)

                                              ccAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGt                                        <  1:2132821/90‑1 (MQ=255)

                                              ccAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGt                                        <  1:1058366/90‑1 (MQ=255)

                                               cAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGtt                                       <  1:2752405/90‑1 (MQ=255)

                                               cAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGtt                                       >  1:1315304/1‑90 (MQ=255)

                                               cAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGtt                                       <  1:664969/90‑1 (MQ=255)

                                                 aGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTa                                     >  2:1916357/1‑90 (MQ=255)

                                                 aGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTa                                     >  1:1942441/1‑90 (MQ=255)

                                                  gTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTaa                                    <  1:2427549/90‑1 (MQ=255)

                                                  gTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTaa                                    >  2:222542/1‑90 (MQ=255)

                                                      cacaACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGt                                <  1:2433317/90‑1 (MQ=255)

                                                      cacaACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGt                                >  2:1398510/1‑90 (MQ=255)

                                                       acaACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTa                               >  2:1170129/1‑90 (MQ=255)

                                                        caACTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTAt                              <  1:1049888/90‑1 (MQ=255)

                                                         aaCTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTAtt                             >  2:39964/1‑90 (MQ=255)

                                                          aCTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTg                            <  2:1490655/90‑1 (MQ=255)

                                                          aCTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTg                            <  1:2764037/90‑1 (MQ=255)

                                                           cTTTCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGa                           <  2:1368288/90‑1 (MQ=255)

                                                            tttCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGAc                          <  1:2240835/90‑1 (MQ=255)

                                                             ttCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGAcc                         <  2:2476422/90‑1 (MQ=255)

                                                             ttCATCAGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGAcc                         >  1:2158845/1‑90 (MQ=255)

                                                              tcatcaGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCa                        <  2:928575/90‑1 (MQ=255)

                                                               catcaGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCNNGGNAATATCACTGTTAAATTGTTTAATGGTATTGACCAg                       >  2:1560187/1‑90 (MQ=255)

                                                               catcaGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAg                       <  1:1573727/90‑1 (MQ=255)

                                                               catcaGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAg                       <  2:1617757/90‑1 (MQ=255)

                                                               catcaGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAg                       >  2:1577109/1‑90 (MQ=255)

                                                                 tcaGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCa                     >  1:3046335/1‑90 (MQ=255)

                                                                  caGACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCAt                    <  2:2708991/90‑1 (MQ=255)

                                                                    gACTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATgg                  <  1:2840346/90‑1 (MQ=255)

                                                                     aCTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGt                 >  1:2400039/1‑90 (MQ=255)

                                                                     aCTACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGt                 <  2:396455/90‑1 (MQ=255)

                                                                       tACGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTAc               <  2:2255617/90‑1 (MQ=255)

                                                                        aCGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTAcc              >  1:768968/1‑90 (MQ=255)

                                                                         cGTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCt             <  1:1508621/90‑1 (MQ=255)

                                                                          gTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTg            <  1:761440/90‑1 (MQ=255)

                                                                          gTCCGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTg            >  2:2953495/1‑90 (MQ=255)

                                                                            ccGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTGGa          >  1:2738573/1‑90 (MQ=255)

                                                                            ccGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGGAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTGGa          >  2:532448/1‑90 (MQ=255)

                                                                             cGTTTGCCGGCTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTGGAc         >  2:2028282/1‑90 (MQ=255)

                                                                              gtttgccggcttgccgGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTGGACg        >  2:3306539/1‑90 (MQ=255)

                                                                              gtttgccggcttgccgGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTGGACg        >  2:2152923/1‑90 (MQ=255)

                                                                               tttgccggcttgccggTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTGGACGa       <  2:346682/90‑1 (MQ=255)

                                                                                ttgccggcttgccggtACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTGGACGAg      >  2:2179412/1‑90 (MQ=255)

                                                                                  gccggcttgccggtACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTGGACGAGCa    >  1:1834534/1‑90 (MQ=255)

ATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCAGCATGGTACCTGGACGAGCATG  >  AC_000091/2265762‑2265935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`