BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	2,528,475	G→A	G319G (GGC→GGT)	xapB ←	xanthosine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	2,528,475	0	G	A	99.2%	521.9 / NA	129	G319G (GGC→GGT)	xapB	xanthosine transporter
Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (64/63); minor base C (0/1); total (65/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCA  >  AC_000091/2528388‑2528561

gagCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAAcc                                                                                      >  2:81637/1‑90 (MQ=255)

  gCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCAt                                                                                    <  2:605946/90‑1 (MQ=255)

    cccTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATaa                                                                                  <  2:2781359/90‑1 (MQ=255)

     ccTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATaaa                                                                                 >  2:385666/1‑90 (MQ=255)

      cTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAAc                                                                                <  2:2932812/90‑1 (MQ=255)

           cgcTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAatca                                                                           >  1:1037693/1‑90 (MQ=255)

           cgcTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAatca                                                                           >  2:3365959/1‑90 (MQ=255)

            gcTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAatcat                                                                          <  2:2398193/90‑1 (MQ=255)

             cTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAatcatc                                                                         <  2:2310094/90‑1 (MQ=255)

             cTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAatcatc                                                                         >  2:3131432/1‑90 (MQ=255)

             cTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAatcatc                                                                         <  1:3039205/90‑1 (MQ=255)

              tGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCg                                                                        <  1:1784901/90‑1 (MQ=255)

              tGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCg                                                                        <  2:2079257/90‑1 (MQ=255)

              tGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCg                                                                        <  2:860959/90‑1 (MQ=255)

                gCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAc                                                                      <  1:2631911/90‑1 (MQ=255)

                 cACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAca                                                                     <  1:471981/90‑1 (MQ=255)

                 cACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAca                                                                     >  1:3154969/1‑90 (MQ=255)

                   cGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAcagc                                                                   >  1:1558838/1‑90 (MQ=255)

                   cGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAcagc                                                                   <  2:480337/90‑1 (MQ=255)

                   cGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAcagc                                                                   <  1:2211684/90‑1 (MQ=255)

                    gAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAcagca                                                                  >  1:2842825/1‑90 (MQ=255)

                    gAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAcagca                                                                  <  2:2481357/90‑1 (MQ=255)

                    gAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAcagca                                                                  >  2:2061650/1‑90 (MQ=255)

                     aaTGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAcagcag                                                                 <  1:1458627/90‑1 (MQ=255)

                      aTGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAcagcagc                                                                >  2:2863538/1‑90 (MQ=255)

                      aTGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAcagcagc                                                                <  2:1754118/90‑1 (MQ=255)

                       tGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCACCGAcagcagca                                                               <  2:1011936/90‑1 (MQ=255)

                        gCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGAcagcagcag                                                              <  2:3373741/90‑1 (MQ=255)

                         cTGGAATCAACTTCCTGTTGGACAAATGCCGAACCAGAAATAATGAAGAAATCGAATGCACAACCATAAACAATCATCGACagcagcagc                                                             >  2:1566343/1‑90 (MQ=255)

                         cTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACagcagcagc                                                             <  2:1158683/90‑1 (MQ=255)

                         cTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACagcagcagc                                                             >  1:466061/1‑90 (MQ=255)

                          tGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAgcagcagca                                                            <  2:1936587/90‑1 (MQ=255)

                            gAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCaaa                                                          <  2:1558246/90‑1 (MQ=255)

                             aaTCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCaaaa                                                         <  2:858956/90‑1 (MQ=255)

                             aaTCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCaaaa                                                         >  1:2839915/1‑90 (MQ=255)

                              aTCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAaaat                                                        <  2:922230/90‑1 (MQ=255)

                               tCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAaaata                                                       <  2:3390656/90‑1 (MQ=255)

                               tCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAaaata                                                       <  1:2751642/90‑1 (MQ=255)

                                cAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAaaataa                                                      >  2:2574703/1‑90 (MQ=255)

                                 aaCTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAaaataaa                                                     <  1:2190092/90‑1 (MQ=255)

                                  aCTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAaaataaat                                                    <  1:1526206/90‑1 (MQ=255)

                                    ttCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATcc                                                  <  1:3246197/90‑1 (MQ=255)

                                    ttCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATcc                                                  >  1:3200944/1‑90 (MQ=255)

                                      ccTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCgg                                                <  2:3104443/90‑1 (MQ=255)

                                       cTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgt                                               >  2:3130003/1‑90 (MQ=255)

                                       cTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgt                                               >  2:1280294/1‑90 (MQ=255)

                                        tGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgtt                                              <  2:710449/90‑1 (MQ=255)

                                        tGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgtt                                              >  1:877718/1‑90 (MQ=255)

                                         gTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgttg                                             >  1:3238404/1‑90 (MQ=255)

                                           tCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgttgtt                                           >  1:1488139/1‑90 (MQ=255)

                                           tCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGgttgtt                                           >  2:1137234/1‑90 (MQ=255)

                                             gACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGa                                         >  1:3435443/1‑90 (MQ=255)

                                             gACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGa                                         >  2:2512967/1‑90 (MQ=255)

                                              aCAAATTCCGAACCAGAAAGATTGAAGAAATCGAATGCACACCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGAc                                        <  2:1132184/90‑1 (MQ=255)

                                               cAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACg                                       >  1:1140706/1‑90 (MQ=255)

                                               cAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACg                                       <  2:1586808/90‑1 (MQ=255)

                                                aaaTACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACgg                                      >  2:1464918/1‑90 (MQ=255)

                                                aaaTACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACgg                                      <  2:506193/90‑1 (MQ=255)

                                                 aaTACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGa                                     >  2:586911/1‑90 (MQ=255)

                                                 aaTACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGa                                     <  2:2254665/90‑1 (MQ=255)

                                                   tACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATc                                   <  2:18010/90‑1 (MQ=255)

                                                   tACCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATc                                   >  1:1372367/1‑90 (MQ=255)

                                                    aCCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCg                                  <  1:2300853/90‑1 (MQ=255)

                                                    aCCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCg                                  >  2:2770022/1‑90 (MQ=255)

                                                    aCCGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCg                                  >  1:2890134/1‑90 (MQ=255)

                                                     ccGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGc                                 >  2:603076/1‑90 (MQ=255)

                                                      cGAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGcc                                >  1:2542501/1‑90 (MQ=255)

                                                       gAACCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCa                               <  1:1125837/90‑1 (MQ=255)

                                                         aCCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATa                             >  1:2874359/1‑90 (MQ=255)

                                                         aCCAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATa                             >  2:747676/1‑90 (MQ=255)

                                                          ccAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAg                            <  2:1712811/90‑1 (MQ=255)

                                                           cAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAgg                           <  1:1685214/90‑1 (MQ=255)

                                                           cAGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAgg                           >  2:1444412/1‑90 (MQ=255)

                                                            aGAAATATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGc                          >  2:1338720/1‑90 (MQ=255)

                                                               aaTATTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCgaa                       <  2:572349/90‑1 (MQ=255)

                                                                atatTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCgaag                      >  1:2011105/1‑90 (MQ=255)

                                                                 tatTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCgaaga                     >  2:2849736/1‑90 (MQ=255)

                                                                  atTGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCgaagaa                    >  2:2818786/1‑90 (MQ=255)

                                                                   ttGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGaagaag                   <  1:937344/90‑1 (MQ=255)

                                                                    tGAAGAAATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGc                  >  2:2032270/1‑90 (MQ=255)

                                                                       agaaATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCaa               >  1:2184337/1‑90 (MQ=255)

                                                                        gaaATCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCaaa              <  2:2734913/90‑1 (MQ=255)

                                                                         aaaTCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAg             <  1:3160656/90‑1 (MQ=255)

                                                                         aaaTCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAg             <  1:646715/90‑1 (MQ=255)

                                                                          aaTCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAgc            <  2:2608535/90‑1 (MQ=255)

                                                                           aTCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAgcg           <  1:3373038/90‑1 (MQ=255)

                                                                            tCGAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAACTAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAgcgc          <  2:1827931/90‑1 (MQ=255)

                                                                             cGAGTGCACAACCATAAACAATTATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCa         <  2:1210930/90‑1 (MQ=255)

                                                                             cGAATGCACAACTATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCa         >  2:3028967/1‑90 (MQ=255)

                                                                              gAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAg        <  1:1147205/90‑1 (MQ=255)

                                                                              gAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAg        >  1:329618/1‑90 (MQ=255)

                                                                              gAATGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAg        <  2:1462075/90‑1 (MQ=255)

                                                                               aaTGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGc       >  1:3247400/1‑90 (MQ=255)

                                                                                aTGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCg      >  1:1989920/1‑90 (MQ=255)

                                                                                 tGCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGt     >  1:3393385/1‑90 (MQ=255)

                                                                                  gCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTc    >  1:129742/1‑90 (MQ=255)

                                                                                  gCACAACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTGGACGGATCGCCATAGGCGAAGAAGCCAAAGCGTAGGGTc    >  2:1789574/1‑90 (MQ=255)

                                                                                   cacaACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTcc   <  1:3100522/90‑1 (MQ=255)

                                                                                   cacaACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTcc   >  1:380749/1‑90 (MQ=255)

                                                                                   cacaACCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTcc   <  2:1507794/90‑1 (MQ=255)

GAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCA  >  AC_000091/2528388‑2528561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`