BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	104,666	A→G	T229A (ACT→GCT)	ftsA →	ATP‑binding cell division protein involved in recruitment of FtsK to Z ring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	104,666	0	A	G	100.0%	528.2 / NA	135	T229A (ACT→GCT)	ftsA	ATP‑binding cell division protein involved in recruitment of FtsK to Z ring
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (62/73); total (62/73)

Only 100 of 136 total aligned reads displayed.

GATGAACGTGAACTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACACTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGCGAT  >  AC_000091/104579‑104751

gATGAACGTGAACTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCt                                                                                     >  1:540952/1‑90 (MQ=255)

 aTGAACGTGAACTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTa                                                                                    >  1:2321671/1‑90 (MQ=255)

   gAACGTGAACTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAg                                                                                  <  2:256096/90‑1 (MQ=255)

    aaCGTGAACTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAgg                                                                                 >  2:916310/1‑90 (MQ=255)

    aaCGTGAACTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAgg                                                                                 <  1:3370945/90‑1 (MQ=255)

      cGTGAACTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTa                                                                               <  1:521398/90‑1 (MQ=255)

       gTGAACTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTaa                                                                              >  1:57335/1‑90 (MQ=255)

       gTGAACTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTaa                                                                              >  2:1379372/1‑90 (MQ=255)

          aaCTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTc                                                                           >  1:3151288/1‑90 (MQ=255)

          aaCTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTc                                                                           <  2:1296420/90‑1 (MQ=255)

           aCTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTcc                                                                          <  2:2471601/90‑1 (MQ=255)

           aCTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTcc                                                                          <  1:1804850/90‑1 (MQ=255)

            cTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCt                                                                         >  1:563861/1‑90 (MQ=255)

             tGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCtt                                                                        >  2:2725966/1‑90 (MQ=255)

              gggTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTa                                                                       >  1:2812657/1‑90 (MQ=255)

               ggTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTAt                                                                      <  2:146468/90‑1 (MQ=255)

                 tgtCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGc                                                                    <  1:2214091/90‑1 (MQ=255)

                  gtCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCt                                                                   >  2:948382/1‑90 (MQ=255)

                   tCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTg                                                                  <  1:2048393/90‑1 (MQ=255)

                   tCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTg                                                                  <  1:2476207/90‑1 (MQ=255)

                     tGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATGCCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGc                                                                <  2:2861198/90‑1 (MQ=255)

                      gCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCa                                                               <  2:1917127/90‑1 (MQ=255)

                      gCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCa                                                               <  1:303450/90‑1 (MQ=255)

                      gCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCa                                                               <  1:1205798/90‑1 (MQ=255)

                      gCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGGGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCa                                                               <  2:483060/90‑1 (MQ=255)

                       cgtcgtCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCaa                                                              >  2:3154960/1‑90 (MQ=255)

                        gtcgtcGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAAt                                                             >  1:462250/1‑90 (MQ=255)

                        gtcgtcGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAAt                                                             >  2:1554032/1‑90 (MQ=255)

                        gtcgtcGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAAt                                                             <  2:2747495/90‑1 (MQ=255)

                         tcgtcgATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATg                                                            >  2:789574/1‑90 (MQ=255)

                           gtcgATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGNNTTNCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTc                                                          >  2:2088127/1‑90 (MQ=255)

                           gtcgATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTc                                                          >  2:2181464/1‑90 (MQ=255)

                           gtcgATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTc                                                          <  2:1542944/90‑1 (MQ=255)

                           gtcgATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTc                                                          >  1:1538130/1‑90 (MQ=255)

                            tcgATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCg                                                         <  2:2959486/90‑1 (MQ=255)

                              gATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTg                                                       >  1:2478975/1‑90 (MQ=255)

                              gATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTg                                                       >  2:869476/1‑90 (MQ=255)

                              gATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTg                                                       >  1:1999937/1‑90 (MQ=255)

                               atatCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGa                                                      <  1:651274/90‑1 (MQ=255)

                                 atCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGAcc                                                    <  2:1865541/90‑1 (MQ=255)

                                 atCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGAcc                                                    >  2:2262324/1‑90 (MQ=255)

                                  tCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCa                                                   >  2:80167/1‑90 (MQ=255)

                                  tCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCa                                                   >  1:633703/1‑90 (MQ=255)

                                     gttgtggtACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTg                                                <  2:2361257/87‑1 (MQ=255)

                                     gtggtggtACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTg                                                >  2:3186481/1‑90 (MQ=255)

                                      tggtggtACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGa                                               >  2:1302942/1‑90 (MQ=255)

                                      tggtggtACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGa                                               <  2:215844/90‑1 (MQ=255)

                                       ggtggtACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGat                                              >  1:2686547/1‑90 (MQ=255)

                                         tggtACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGatat                                            <  2:1699099/90‑1 (MQ=255)

                                            tACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGc                                         >  1:1872974/1‑90 (MQ=255)

                                            tACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCAACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGc                                         >  2:3153066/1‑90 (MQ=255)

                                              cAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCtt                                       <  1:2072784/90‑1 (MQ=255)

                                              cAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCtt                                       <  1:1026615/90‑1 (MQ=255)

                                              cAATGGATATCGCCGTTTAGACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCtt                                       <  1:1115226/90‑1 (MQ=255)

                                               aaTGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTa                                      <  1:3447881/90‑1 (MQ=255)

                                               aaTGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTa                                      >  1:2083022/1‑90 (MQ=255)

                                                aTGGATATCGCCGTTTATGCCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTAc                                     <  2:2531705/90‑1 (MQ=255)

                                                aTGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTAc                                     >  2:2555546/1‑90 (MQ=255)

                                                aTGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTAc                                     >  1:1041155/1‑90 (MQ=255)

                                                  ggATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGc                                   <  1:3209128/90‑1 (MQ=255)

                                                  ggATATCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGc                                   >  1:3410234/1‑90 (MQ=255)

                                                     tatCGGCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCtt                                >  2:1780059/1‑90 (MQ=255)

                                                     tatCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCtt                                <  2:393661/90‑1 (MQ=255)

                                                     tatCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCtt                                >  2:2717557/1‑90 (MQ=255)

                                                       tCGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTg                              <  2:2976770/90‑1 (MQ=255)

                                                        cGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTgg                             >  1:2261718/1‑90 (MQ=255)

                                                        cGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTgg                             <  1:1397673/90‑1 (MQ=255)

                                                        cGCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTgg                             <  1:2500425/90‑1 (MQ=255)

                                                         gCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGc                            >  1:3447455/1‑90 (MQ=255)

                                                         gCCGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGc                            <  1:2279986/90‑1 (MQ=255)

                                                          ccGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCa                           >  1:1231087/1‑90 (MQ=255)

                                                          ccGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCa                           >  1:1989409/1‑90 (MQ=255)

                                                           cGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCAc                          >  2:127606/1‑90 (MQ=255)

                                                           cGTTTATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCAc                          <  2:1395689/90‑1 (MQ=255)

                                                              ttATACCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCAcgcc                       <  1:1990195/90‑1 (MQ=255)

                                                                ataCCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCAcgccgc                     >  2:110504/1‑90 (MQ=255)

                                                                 taCCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACgccgcc                    >  1:880483/1‑90 (MQ=255)

                                                                 taCCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACgccgcc                    >  1:1817423/1‑90 (MQ=255)

                                                                  aCCGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCa                   <  2:3354463/90‑1 (MQ=255)

                                                                   ccGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCaa                  >  1:1287261/1‑90 (MQ=255)

                                                                    cGGTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAg                 <  2:2506527/90‑1 (MQ=255)

                                                                     ggTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGc                <  2:3326557/90‑1 (MQ=255)

                                                                      gtagggTATTGCGCCACGCTAAGGGAATTCCTTATTCTGGCAATGTCGAGACCAGTGATATCGTTTACGCCTTTGGCACGCCGCCAAGCg               >  2:834878/4‑90 (MQ=255)

                                                                      gTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCg               <  2:851004/90‑1 (MQ=255)

                                                                      gTGGGGCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCg               <  2:2979946/90‑1 (MQ=255)

                                                                        ggggCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGAc             <  2:1573546/90‑1 (MQ=255)

                                                                        ggggCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGAc             <  2:2605990/90‑1 (MQ=255)

                                                                        ggggCATTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGAc             >  1:1693432/1‑90 (MQ=255)

                                                                             aTTGCGCCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGa        <  1:225940/90‑1 (MQ=255)

                                                                                gcgcCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGc     <  2:946568/90‑1 (MQ=255)

                                                                                gcgcCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGc     >  1:740411/1‑90 (MQ=255)

                                                                                gcgcCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGc     <  2:368874/90‑1 (MQ=255)

                                                                                 cgcCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGCg    <  1:1410458/90‑1 (MQ=255)

                                                                                 cgcCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGCg    >  2:2244813/1‑90 (MQ=255)

                                                                                  gcCACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGCGa   >  2:1097913/1‑90 (MQ=255)

                                                                                   ccACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGCGAt  <  1:1841606/90‑1 (MQ=255)

                                                                                   ccACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGCGAt  <  1:3427418/90‑1 (MQ=255)

                                                                                   ccACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGCGAt  >  2:1971812/1‑90 (MQ=255)

                                                                                   ccACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGCGAt  <  2:287157/90‑1 (MQ=255)

                                                                                   ccACGCTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGCGAt  >  1:3137153/1‑90 (MQ=255)

GATGAACGTGAACTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTTTATACCGGTGGGGCATTGCGCCACACTAAGGTAATTCCTTATGCTGGCAATGTCGTGACCAGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGCGAT  >  AC_000091/104579‑104751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`