BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	2,634,430	T→C	Q14R (CAG→CGG)	yfgJ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	2,634,430	0	T	C	100.0%	474.0 / NA	120	Q14R (CAG→CGG)	yfgJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (47/73); total (47/73)

Only 100 of 122 total aligned reads displayed.

ACCTGCAACGGTTGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCTGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAGGGAATG  >  AC_000091/2634343‑2634516

acCTGCAACGGTTGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGa                                                                                      <  1:2365153/90‑1 (MQ=255)

  cTGCAACGGTTGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATc                                                                                    <  2:1513125/90‑1 (MQ=255)

  cTGCAACGGTTGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATc                                                                                    >  1:3479372/1‑90 (MQ=255)

  cTGCAACGGTTGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATc                                                                                    <  1:3155463/90‑1 (MQ=255)

   tGCAACGGTTGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCTGCAACGCGCATGGCCGTTATCCCGATCa                                                                                   >  2:1753805/1‑90 (MQ=255)

    gCAACGGTTGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCaa                                                                                  <  2:1168790/90‑1 (MQ=255)

     cAACGGTTGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAg                                                                                 >  1:3306664/1‑90 (MQ=255)

        cGGTTGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGcac                                                                              <  2:3278608/90‑1 (MQ=255)

         ggTTGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGcaca                                                                             <  1:1163890/90‑1 (MQ=255)

           ttGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATg                                                                           <  1:3173676/90‑1 (MQ=255)

             gATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCt                                                                         >  1:1028705/1‑90 (MQ=255)

             gATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCt                                                                         <  2:3113541/90‑1 (MQ=255)

             gATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCt                                                                         <  2:3468799/90‑1 (MQ=255)

              aTGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTg                                                                        >  2:1044162/1‑90 (MQ=255)

              aTGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTg                                                                        <  2:2109480/90‑1 (MQ=255)

               tGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGa                                                                       >  1:1598705/1‑90 (MQ=255)

                 gCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACa                                                                     <  1:1505458/90‑1 (MQ=255)

                   aGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACAtt                                                                   >  1:3244120/1‑90 (MQ=255)

                   aGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACAtt                                                                   >  1:3155529/1‑90 (MQ=255)

                   aGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACAtt                                                                   >  2:2745455/1‑90 (MQ=255)

                    gTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATtg                                                                  >  2:1162888/1‑90 (MQ=255)

                    gTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATtg                                                                  <  1:2948198/90‑1 (MQ=255)

                    gTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATtg                                                                  <  1:2533699/90‑1 (MQ=255)

                     tCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATtgt                                                                 <  1:1436506/90‑1 (MQ=255)

                      cTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATtgtg                                                                >  2:150556/1‑90 (MQ=255)

                       tGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTgg                                                               >  2:302903/1‑90 (MQ=255)

                       tGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTgg                                                               <  2:1166653/90‑1 (MQ=255)

                       tGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTgg                                                               >  1:964937/1‑90 (MQ=255)

                           cAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACaa                                                           <  2:2342659/90‑1 (MQ=255)

                            aaaGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAAt                                                          >  1:1983191/1‑90 (MQ=255)

                             aaGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATg                                                         >  2:2198360/1‑90 (MQ=255)

                             aaGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATg                                                         <  2:694233/90‑1 (MQ=255)

                             aaGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATg                                                         >  2:860510/1‑90 (MQ=255)

                              agagCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGc                                                        >  1:913934/1‑90 (MQ=255)

                              agagCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGc                                                        <  2:619063/90‑1 (MQ=255)

                              agagCTTTCATTTCAATAAATCCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGc                                                        <  1:3248488/90‑1 (MQ=255)

                               gagCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCa                                                       <  1:3133574/90‑1 (MQ=255)

                                 gCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGt                                                     <  2:909454/90‑1 (MQ=255)

                                 gCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGt                                                     <  2:2526610/90‑1 (MQ=255)

                                  cTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGTCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGtt                                                    <  2:1951526/90‑1 (MQ=255)

                                  cTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGtt                                                    >  1:596263/1‑90 (MQ=255)

                                  cTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGtt                                                    <  2:2557801/90‑1 (MQ=255)

                                    ttcatttcaATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTcc                                                  <  2:480280/90‑1 (MQ=255)

                                    ttcatttcaATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTcc                                                  <  2:3279861/90‑1 (MQ=255)

                                    ttcatttcaATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTcc                                                  <  1:1963690/90‑1 (MQ=255)

                                     tcatttcaATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCa                                                 <  2:1001334/90‑1 (MQ=255)

                                        tttcaATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACTATGCTGTTCCatat                                              <  2:3194887/90‑1 (MQ=255)

                                        tttcaATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCatat                                              <  1:1121355/90‑1 (MQ=255)

                                          tcaATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGa                                            <  1:410845/90‑1 (MQ=255)

                                           caATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGaa                                           <  2:512899/90‑1 (MQ=255)

                                               aaaTTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATtcc                                       >  2:2980537/1‑90 (MQ=255)

                                               aaaTTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATtcc                                       <  1:2346329/90‑1 (MQ=255)

                                                aaTTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATtcct                                      <  2:1736292/90‑1 (MQ=255)

                                                aaTTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATtcct                                      >  1:2673930/1‑90 (MQ=255)

                                                aaTTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATCGTGGACAATGCAGTTCCATATGAATtcct                                      <  2:3388655/90‑1 (MQ=255)

                                                  ttCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATtcctcc                                    >  1:2933046/1‑90 (MQ=255)

                                                  ttCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATtcctcc                                    >  1:1743131/1‑90 (MQ=255)

                                                  ttCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATtcctcc                                    <  2:1096842/90‑1 (MQ=255)

                                                   tCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCg                                   <  1:3333754/90‑1 (MQ=255)

                                                      ccGCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTa                                >  1:3090423/1‑90 (MQ=255)

                                                        gCAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGc                              <  1:709185/90‑1 (MQ=255)

                                                         cAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGcc                             >  2:2965210/1‑90 (MQ=255)

                                                         cAGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGcc                             <  1:1061532/90‑1 (MQ=255)

                                                          aGGATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCa                            <  1:1178285/90‑1 (MQ=255)

                                                           ggATCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCAt                           >  2:1921335/1‑90 (MQ=255)

                                                             aTCGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTc                         <  1:928186/90‑1 (MQ=255)

                                                               cGGCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTccc                       >  1:1723175/1‑90 (MQ=255)

                                                                ggCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCct                      >  1:1901957/1‑90 (MQ=255)

                                                                ggCAACGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCct                      >  1:1332437/1‑90 (MQ=255)

                                                                   aaCGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTa                   <  1:1918453/90‑1 (MQ=255)

                                                                   aaCGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTa                   >  1:2558562/1‑90 (MQ=255)

                                                                   aaCGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTa                   <  1:430261/90‑1 (MQ=255)

                                                                   aaCGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTa                   <  2:253292/90‑1 (MQ=255)

                                                                    aCGCGCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTAc                  >  1:1283380/1‑90 (MQ=255)

                                                                     cgcgCATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACa                 <  2:1512429/90‑1 (MQ=255)

                                                                      gcgcATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACAt                <  2:2798858/90‑1 (MQ=255)

                                                                      gcgcATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACAt                <  2:3357344/90‑1 (MQ=255)

                                                                        gcATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACAtta              <  2:354711/90‑2 (MQ=255)

                                                                        gcATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTc              >  1:1724472/1‑90 (MQ=255)

                                                                        gcATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTc              >  2:3285218/1‑90 (MQ=255)

                                                                         cATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCa             <  2:2024055/90‑1 (MQ=255)

                                                                         cATGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCa             >  2:1086513/1‑90 (MQ=255)

                                                                          aTGGCCGTTATCCCGATCAAGCCCATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCAt            <  2:95100/90‑1 (MQ=255)

                                                                          aTGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGGCAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCAt            >  2:2639981/1‑90 (MQ=255)

                                                                           tGGCCTTTATCCCGATCAAGCACATGCTGACATTGTTGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATa           <  2:2308378/90‑1 (MQ=255)

                                                                           tGGCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATa           >  1:2471581/1‑90 (MQ=255)

                                                                             gCCGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATaga         <  1:54903/90‑1 (MQ=255)

                                                                              ccGTTATCCCGATCAAGCACATGCTGACATTGTGGCCAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATacag        >  2:625643/1‑90 (MQ=255)

                                                                               cGTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAgg       <  1:2451165/90‑1 (MQ=255)

                                                                                gTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAggg      >  1:376098/1‑90 (MQ=255)

                                                                                gTTATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAggg      <  1:835033/90‑1 (MQ=255)

                                                                                 ttATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAGGGa     >  1:2231584/1‑90 (MQ=255)

                                                                                 ttATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAGGGa     <  1:2603806/90‑1 (MQ=255)

                                                                                 ttATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAGGGa     <  1:424876/90‑1 (MQ=255)

                                                                                  tATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAGGGaa    >  1:1184431/1‑90 (MQ=255)

                                                                                  tATCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAGGGaa    <  2:941201/90‑1 (MQ=255)

                                                                                   aTCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAGGGAAt   >  1:2046127/1‑90 (MQ=255)

                                                                                   aTCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAGGGAAt   >  1:2519894/1‑90 (MQ=255)

                                                                                    tCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAGGGAATg  <  2:1684130/90‑1 (MQ=255)

                                                                                    tCCCGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAGGGAATg  >  1:217553/1‑90 (MQ=255)

ACCTGCAACGGTTGATGGCAGTCTGGACAAAGAGCTTTCATTTCAATAAATTCCCCGCAGGATCGGCAACGCGCATGGCCGTTATCCTGATCAAGCACATGCTGACATTGTGGACAATGCAGTTCCATATGAATTCCTCCGGTAGCCATTCCCTCTACATTCATAGAGGGAATG  >  AC_000091/2634343‑2634516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`