BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	2,804,247	T→C	D259D (GAT→GAC)	proV →	glycine betaine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	2,804,247	0	T	C	100.0%	665.9 / NA	166	D259D (GAT→GAC)	proV	glycine betaine transporter subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (72/94); total (72/94)

Only 100 of 168 total aligned reads displayed.

TATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAAT  >  AC_000091/2804160‑2804333

tATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTa                                                                                      >  2:2467466/1‑90 (MQ=255)

 aTTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTAt                                                                                     >  1:237060/1‑90 (MQ=255)

 aTTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTAt                                                                                     <  1:1359097/90‑1 (MQ=255)

 aTTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTAt                                                                                     <  2:1182872/90‑1 (MQ=255)

 aTTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTAt                                                                                     >  1:1591554/1‑90 (MQ=255)

 aTTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTAt                                                                                     >  2:1391908/1‑90 (MQ=255)

  ttGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATg                                                                                    >  1:2366987/1‑90 (MQ=255)

  ttGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATg                                                                                    >  1:321523/1‑90 (MQ=255)

   tGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGt                                                                                   <  2:774881/90‑1 (MQ=255)

    ggCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATgcc                                                                                  <  1:3126489/90‑3 (MQ=255)

    ggCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTc                                                                                  >  1:314825/1‑90 (MQ=255)

     gCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGAGAATGACTATGTcc                                                                                 >  2:2521062/1‑90 (MQ=255)

         ccGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTAc                                                                             >  2:230832/1‑90 (MQ=255)

          cGAATTGCCATTATGCAAAATGGTTAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTAcc                                                                            <  2:3370536/90‑1 (MQ=255)

          cGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTAcc                                                                            >  2:612626/1‑90 (MQ=255)

          cGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTAcc                                                                            >  1:1959693/1‑90 (MQ=255)

           gAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACct                                                                           >  1:555044/1‑90 (MQ=255)

           gAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACct                                                                           <  1:426259/90‑1 (MQ=255)

           gAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACct                                                                           >  1:1085262/1‑90 (MQ=255)

           gAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACct                                                                           <  2:3258258/90‑1 (MQ=255)

            aaTTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACctt                                                                          <  1:2854700/90‑1 (MQ=255)

            aaTTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACctt                                                                          <  1:523907/90‑1 (MQ=255)

            aaTTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACctt                                                                          <  1:475376/90‑1 (MQ=255)

            aaTTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACctt                                                                          <  2:2068486/90‑1 (MQ=255)

             aTTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGC‑‑ATGACTATGTCCGTACcttctt                                                                       <  1:2643510/90‑1 (MQ=255)

              ttGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACcttct                                                                        <  2:2903576/90‑1 (MQ=255)

                 ccATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTcc                                                                     >  2:2675818/1‑90 (MQ=255)

                 ccATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTcc                                                                     <  1:1550035/90‑1 (MQ=255)

                 ccATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTcc                                                                     <  2:656770/90‑1 (MQ=255)

                 ccATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTcc                                                                     <  2:2598890/90‑1 (MQ=255)

                  cATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCg                                                                    >  1:7892/1‑90 (MQ=255)

                  cATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCg                                                                    >  1:1149439/1‑90 (MQ=255)

                  cATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCg                                                                    <  2:494738/90‑1 (MQ=255)

                  cATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCg                                                                    >  1:986439/1‑90 (MQ=255)

                   aTTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGt                                                                   >  1:3314854/1‑90 (MQ=255)

                     tATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTgg                                                                 >  2:264572/1‑90 (MQ=255)

                      aTGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGc                                                                <  2:867886/90‑1 (MQ=255)

                      aTGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGc                                                                >  1:2176138/1‑90 (MQ=255)

                       tGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCg                                                               <  1:1403385/90‑1 (MQ=255)

                         cAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGtt                                                             >  2:1095709/1‑90 (MQ=255)

                         cAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGtt                                                             <  1:749972/90‑1 (MQ=255)

                         cAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGtt                                                             <  1:680064/90‑1 (MQ=255)

                           aaaTGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGa                                                           >  2:2231998/1‑90 (MQ=255)

                           aaaTGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGa                                                           >  2:2002882/1‑90 (MQ=255)

                           aaaTGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGa                                                           <  2:958821/90‑1 (MQ=255)

                           aaaTGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGa                                                           >  1:2212078/1‑90 (MQ=255)

                           aaaTGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGa                                                           >  1:2643800/1‑90 (MQ=255)

                            aaTGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGat                                                          >  1:1112465/1‑90 (MQ=255)

                              tGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGatat                                                        >  2:2579139/1‑90 (MQ=255)

                               ggTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATAtt                                                       <  1:194084/90‑1 (MQ=255)

                                gTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTa                                                      <  1:3123038/90‑1 (MQ=255)

                                 tGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAg                                                     <  1:786864/90‑1 (MQ=255)

                                   aaGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTc                                                   >  2:1527816/1‑90 (MQ=255)

                                    aGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCa                                                  >  2:454113/1‑90 (MQ=255)

                                     gTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAg                                                 <  1:1610668/90‑1 (MQ=255)

                                      tGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGGCTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAgg                                                >  1:1190027/1‑90 (MQ=255)

                                      tGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAgg                                                >  1:1762548/1‑90 (MQ=255)

                                       ggTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGt                                               <  1:1423735/90‑1 (MQ=255)

                                       ggTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGt                                               <  2:1463523/90‑1 (MQ=255)

                                       ggTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGt                                               >  1:1617400/1‑90 (MQ=255)

                                        gTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTa                                              <  1:851943/90‑1 (MQ=255)

                                        gTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTa                                              <  2:782077/90‑1 (MQ=255)

                                        gTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTa                                              <  1:2291288/90‑1 (MQ=255)

                                         tACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTAt                                             <  2:309340/90‑1 (MQ=255)

                                         tACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTAt                                             <  1:3438807/90‑1 (MQ=255)

                                          aCAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTAtt                                            <  2:23178/90‑1 (MQ=255)

                                          aCAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTAtt                                            >  2:1668702/1‑90 (MQ=255)

                                           cAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTc                                           <  2:1687543/90‑1 (MQ=255)

                                            aGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCa                                          >  2:1693126/1‑90 (MQ=255)

                                            aGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCa                                          >  1:337103/1‑90 (MQ=255)

                                               tCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTg                                       <  2:609796/90‑1 (MQ=255)

                                                cGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGc                                      <  1:2203094/90‑1 (MQ=255)

                                                 ggCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCg                                     <  1:2707072/90‑1 (MQ=255)

                                                 ggCACACCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCg                                     >  1:1271681/1‑90 (MQ=255)

                                                    acacCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGaaa                                  <  2:2102720/90‑1 (MQ=255)

                                                     cacCGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAg                                 >  1:1119944/1‑90 (MQ=255)

                                                       ccGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGat                               <  2:3460390/90‑1 (MQ=255)

                                                        cGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGata                              >  2:2509351/1‑90 (MQ=255)

                                                        cGGATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGata                              <  1:3175652/90‑1 (MQ=255)

                                                          gATGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATAtt                            <  2:2785849/90‑1 (MQ=255)

                                                           aTGAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTg                           >  2:2263198/1‑90 (MQ=255)

                                                             gAAATTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGcc                         >  1:600517/1‑90 (MQ=255)

                                                              aaaTTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGccc                        <  1:868353/90‑1 (MQ=255)

                                                               aaTTCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCccg                       <  2:3235709/90‑1 (MQ=255)

                                                                 ttCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCccgcc                     <  1:2021039/90‑1 (MQ=255)

                                                                 ttCTCAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCccgcc                     >  1:1262762/1‑90 (MQ=255)

                                                                  tctcAATAATCCGGCGAATTACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCccgccg                    <  1:2491969/90‑1 (MQ=255)

                                                                  tctcAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCccgccg                    >  1:3022094/1‑90 (MQ=255)

                                                                     cAATAATCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGac                 >  1:1756557/1‑90 (MQ=255)

                                                                      aataatCCGGCGAATGACTATGTCCGTACCTTCTTCNGTNNCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGaca                <  2:2628847/90‑1 (MQ=255)

                                                                      aataatCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGaca                <  2:2932929/90‑1 (MQ=255)

                                                                       ataatCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGacac               >  1:2810234/1‑90 (MQ=255)

                                                                         aatCCGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCg             <  2:1957901/90‑1 (MQ=255)

                                                                             cGGCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATg         <  1:628743/90‑1 (MQ=255)

                                                                              ggCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATgg        <  1:1758714/90‑1 (MQ=255)

                                                                              ggCGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATgg        <  2:1922547/90‑1 (MQ=255)

                                                                                cGAATGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAATATATTGCCCGCCGGACACCGAATGGCt      <  2:2059496/90‑1 (MQ=255)

                                                                                  aaTGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTa    <  1:821236/90‑1 (MQ=255)

                                                                                   aTGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTaa   <  2:1091282/90‑1 (MQ=255)

                                                                                    tGACTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAAt  <  1:1061317/90‑1 (MQ=255)

TATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAAT  >  AC_000091/2804160‑2804333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`