BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	124,632	A→G	Y539C (TAC→TGC)	aceE →	pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	124,632	0	A	G	100.0%	557.5 / NA	144	Y539C (TAC→TGC)	aceE	pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (75/69); total (75/69)

Only 100 of 145 total aligned reads displayed.

GTCGATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGG  >  AC_000091/124546‑124718

gTCGATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAg                                                                                     <  1:2178861/90‑1 (MQ=255)

  cGATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGcc                                                                                   <  1:2982174/90‑1 (MQ=255)

   gATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGccc                                                                                  <  2:1929360/90‑1 (MQ=255)

   gATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGccc                                                                                  <  2:2982986/90‑1 (MQ=255)

   gATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGccc                                                                                  <  2:2143353/90‑1 (MQ=255)

   gATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGGATGGAAGGGCTGTTCCGGCAGATTGGTATTTGCAGccc                                                                                  >  1:763744/1‑90 (MQ=255)

      cAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGaa                                                                               <  1:1114795/90‑1 (MQ=255)

      cAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGaa                                                                               >  2:2298317/1‑90 (MQ=255)

          gATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGt                                                                           >  2:873581/1‑90 (MQ=255)

            tCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTca                                                                         >  2:2677215/1‑90 (MQ=255)

             cGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTcag                                                                        >  2:947178/1‑90 (MQ=255)

             cGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTcag                                                                        >  1:2620395/1‑90 (MQ=255)

              gTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTcagc                                                                       >  1:1519636/1‑90 (MQ=255)

                  ggTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTa                                                                   <  2:2101680/90‑1 (MQ=255)

                  ggTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTa                                                                   <  2:3086608/90‑1 (MQ=255)

                   gTACCGATCATCGCCGACGAAGCGCGTGCTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTac                                                                  <  2:1475803/90‑1 (MQ=255)

                   gTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTac                                                                  >  1:1784329/1‑90 (MQ=255)

                   gTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTac                                                                  <  1:3386313/90‑1 (MQ=255)

                   gTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTac                                                                  <  2:496936/90‑1 (MQ=255)

                   gTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGCTATTTGCAGCCCGAACGGTCAGCAGTac                                                                  >  2:2386472/1‑90 (MQ=255)

                    tACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTaca                                                                 >  1:3319522/1‑90 (MQ=255)

                    tACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTaca                                                                 >  2:2014509/1‑90 (MQ=255)

                     aCCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTacac                                                                >  2:351899/1‑90 (MQ=255)

                      ccGATCATCGCCGACGAAGTGCGTACTTTCGGTATGGCAGGTCTATTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACAcc                                                               <  1:993163/90‑1 (MQ=255)

                       cGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGGCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACAccc                                                              >  2:265523/1‑90 (MQ=255)

                        gATCATCGCCGACGATGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCCGTACAcccc                                                             <  2:1257265/90‑1 (MQ=255)

                        gATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACAcccc                                                             <  2:2975878/90‑1 (MQ=255)

                        gATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACAcccc                                                             <  1:1281823/90‑1 (MQ=255)

                           catcGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCa                                                          <  1:2615281/90‑1 (MQ=255)

                            atcGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAg                                                         <  1:1155602/90‑1 (MQ=255)

                            atcGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAg                                                         <  1:3224801/90‑1 (MQ=255)

                            atcGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAg                                                         >  1:607329/1‑90 (MQ=255)

                            atcGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAg                                                         >  2:2687183/1‑90 (MQ=255)

                             tcGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAgg                                                        >  2:2889057/1‑90 (MQ=255)

                             tcGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAgg                                                        <  2:374265/90‑1 (MQ=255)

                              cGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGa                                                       <  2:2080862/90‑1 (MQ=255)

                               gCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGAc                                                      >  1:3119703/1‑90 (MQ=255)

                                  gacgaAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACcgc                                                   >  1:1327181/1‑90 (MQ=255)

                                   acgaAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCCGGACcgcg                                                  >  1:1834645/1‑90 (MQ=255)

                                   acgaAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACcgcg                                                  >  2:1619706/1‑90 (MQ=255)

                                    cgaAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGa                                                 <  1:2165365/90‑1 (MQ=255)

                                    cgaAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGa                                                 >  2:1383343/1‑90 (MQ=255)

                                     gaAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAg                                                >  1:1305489/1‑90 (MQ=255)

                                     gaAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAg                                                >  1:831172/1‑90 (MQ=255)

                                      aaGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGc                                               >  2:786124/1‑90 (MQ=255)

                                      aaGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGc                                               >  1:849766/1‑90 (MQ=255)

                                      aaGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGc                                               <  2:3424309/90‑1 (MQ=255)

                                      aaGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATCTGCAGCTCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGc                                               <  2:1016696/90‑1 (MQ=255)

                                       aGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCa                                              >  2:1559230/1‑90 (MQ=255)

                                        gcgcGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAg                                             <  1:898668/90‑1 (MQ=255)

                                          gcgTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGt                                           >  1:1369714/1‑90 (MQ=255)

                                           cgTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGtt                                          >  1:896294/1‑90 (MQ=255)

                                           cgTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGtt                                          <  1:2605826/90‑1 (MQ=255)

                                            gTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTg                                         >  2:635608/1‑90 (MQ=255)

                                            gTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTg                                         <  1:1563906/90‑1 (MQ=255)

                                            gTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTg                                         >  2:2786936/1‑90 (MQ=255)

                                            gTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTg                                         <  2:1861209/90‑1 (MQ=255)

                                              aCTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCt                                       >  1:2262033/1‑90 (MQ=255)

                                               cTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCtt                                      <  2:218478/90‑1 (MQ=255)

                                               cTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCtt                                      <  2:2327015/90‑1 (MQ=255)

                                                  tCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACt                                   >  1:666626/1‑90 (MQ=255)

                                                  tCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACt                                   >  2:3134661/1‑90 (MQ=255)

                                                  tCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACt                                   >  2:3226212/1‑90 (MQ=255)

                                                    ggTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACtat                                 <  2:334026/90‑1 (MQ=255)

                                                    ggTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACtat                                 >  1:2775584/1‑90 (MQ=255)

                                                     gTATGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACtata                                >  2:1804223/1‑90 (MQ=255)

                                                        tGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAaag                             >  2:453282/1‑90 (MQ=255)

                                                        tGGAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAaag                             <  1:3289930/90‑1 (MQ=255)

                                                         ggAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAaaga                            <  2:1122905/90‑1 (MQ=255)

                                                         ggAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAaaga                            >  2:220977/1‑90 (MQ=255)

                                                          gAAGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAaagaa                           <  1:926822/90‑1 (MQ=255)

                                                           aaGGTCTGTTCCGGCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAaagaag                          <  2:1917488/90‑1 (MQ=255)

                                                            aGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAagaaga                         <  1:1187240/90‑1 (MQ=255)

                                                            aGGTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAagaaga                         <  1:3116040/90‑1 (MQ=255)

                                                             ggTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACAGCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGAc                        <  1:486037/90‑1 (MQ=255)

                                                              gTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACg                       >  1:2922442/1‑90 (MQ=255)

                                                              gTCTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACg                       <  1:2853221/90‑1 (MQ=255)

                                                                cTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACgag                     >  1:1781822/1‑90 (MQ=255)

                                                                cTGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACgag                     >  1:250252/1‑90 (MQ=255)

                                                                 tGTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACgaga                    <  2:2783016/90‑1 (MQ=255)

                                                                  gTTCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGaa                   <  2:1049784/90‑1 (MQ=255)

                                                                   ttCCGTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGaaa                  <  2:22175/90‑1 (MQ=255)

                                                                       gTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTc              <  1:785338/90‑1 (MQ=255)

                                                                       gTCAGATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTc              >  2:3232986/1‑90 (MQ=255)

                                                                        tccgATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCa             <  2:1902221/87‑1 (MQ=255)

                                                                         cAGATTGGAATTTGCAACCCGCACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTCGCTAACCATAAAGAAGACGAGAAAGGTCAg            >  2:608539/1‑90 (MQ=255)

                                                                           gATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGAt          <  1:2468168/90‑1 (MQ=255)

                                                                           gATTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGAt          >  1:964556/1‑90 (MQ=255)

                                                                            aTTGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGAtt         >  1:2368155/1‑90 (MQ=255)

                                                                             ttGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTc        >  2:2641889/1‑90 (MQ=255)

                                                                              tGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCt       <  2:3347213/90‑1 (MQ=255)

                                                                              tGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCt       >  1:2772126/1‑90 (MQ=255)

                                                                              tGGTATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCt       >  1:3435400/1‑90 (MQ=255)

                                                                              tGGTATTTGCAGCCCGAACGGTCAGCAGGACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCt       >  2:2013330/1‑90 (MQ=255)

                                                                                 tATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCa    <  1:805386/90‑1 (MQ=255)

                                                                                 tATTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCa    >  2:957835/1‑90 (MQ=255)

                                                                                  aTTTGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAg   >  1:2519712/1‑90 (MQ=255)

                                                                                   tttGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAgg  >  1:2259099/1‑90 (MQ=255)

                                                                                   tttGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAgg  <  1:4705/90‑1 (MQ=255)

                                                                                   tttGCAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGACAAAGGTCAGATTCTGCAgg  <  1:3021169/90‑1 (MQ=255)

GTCGATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTCCGTCAGATTGGTATTTACAGCCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGG  >  AC_000091/124546‑124718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`