BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	2,937,297	G→A	A402T (GCC→ACC)	fucK →	L‑fuculokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	2,937,297	0	G	A	99.2%	487.5 / NA	128	A402T (GCC→ACC)	fucK	L‑fuculokinase
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (52/74); minor base C (0/1); total (52/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

GGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCGGTAAA  >  AC_000091/2937210‑2937382

gggCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGAcc                                                                                     <  1:2589037/90‑1 (MQ=255)

 ggCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACct                                                                                    <  1:212678/90‑1 (MQ=255)

  gCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACctc                                                                                   <  1:2258079/90‑1 (MQ=255)

  gCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACctc                                                                                   <  1:1307986/90‑1 (MQ=255)

  gCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACctc                                                                                   >  1:1305007/1‑90 (MQ=255)

   cATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACctct                                                                                  <  1:2211928/90‑1 (MQ=255)

   cATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACctct                                                                                  <  1:600633/90‑1 (MQ=255)

     tttCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGa                                                                                <  2:2661011/90‑1 (MQ=255)

     tttCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGa                                                                                <  2:1665670/90‑1 (MQ=255)

         tATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTTCAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAatta                                                                            <  2:2040409/90‑1 (MQ=255)

         tATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAatta                                                                            >  2:1804961/1‑90 (MQ=255)

             gcgcgGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGt                                                                        <  2:2921207/90‑1 (MQ=255)

               gcggcgCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTa                                                                      >  1:1680654/1‑90 (MQ=255)

                    gcTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCgg                                                                 <  2:3464742/90‑1 (MQ=255)

                     cTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGt                                                                <  1:2927262/90‑1 (MQ=255)

                      tGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTg                                                               >  2:651697/1‑90 (MQ=255)

                      tGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTg                                                               >  1:2219653/1‑90 (MQ=255)

                       ggAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTgg                                                              >  2:2289233/1‑90 (MQ=255)

                       ggAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTgg                                                              <  1:2434381/90‑1 (MQ=255)

                       ggAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTgg                                                              >  2:298414/1‑90 (MQ=255)

                        gAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTgga                                                             >  2:1053144/1‑90 (MQ=255)

                        gAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTgga                                                             <  2:2931326/90‑1 (MQ=255)

                         aaGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTggag                                                            <  1:252435/90‑1 (MQ=255)

                          aGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTggagg                                                           >  1:1985858/1‑90 (MQ=255)

                            ggTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGCCCTCTGAATTATTGTTAGTCGGTGGAGGaa                                                         <  1:1476206/90‑1 (MQ=255)

                            ggTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGaa                                                         <  1:3374492/90‑1 (MQ=255)

                            ggTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGaa                                                         <  1:144961/90‑1 (MQ=255)

                             gTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAg                                                        >  2:768496/1‑90 (MQ=255)

                              ttAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGt                                                       >  1:2633927/1‑90 (MQ=255)

                              ttAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGt                                                       <  2:3057692/90‑1 (MQ=255)

                              ttAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGt                                                       <  1:3469865/90‑1 (MQ=255)

                               tAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTc                                                      >  2:1550255/1‑90 (MQ=255)

                                aaCTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCg                                                     >  2:1657210/1‑90 (MQ=255)

                                aaCTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCg                                                     <  2:2185942/90‑1 (MQ=255)

                                aaCTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCg                                                     >  2:1840959/1‑90 (MQ=255)

                                   tGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCaa                                                  <  2:2573880/90‑1 (MQ=255)

                                       cAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAAcaca                                              >  2:1072038/1‑90 (MQ=255)

                                       cAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAAcaca                                              >  2:1912904/1‑90 (MQ=255)

                                       cAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAAcaca                                              >  1:1513422/1‑90 (MQ=255)

                                        aaTTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACAt                                             >  2:2316627/1‑90 (MQ=255)

                                        aaTTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACAt                                             <  2:491518/90‑1 (MQ=255)

                                         aTTACAGCGCAATCTACAGATGCTGGAAAAAATCTGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACAtt                                            <  2:2114526/90‑1 (MQ=255)

                                         aTTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACAtt                                            <  2:2456594/90‑1 (MQ=255)

                                          ttACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATtg                                           <  1:2798616/90‑1 (MQ=255)

                                            aCAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATtgtg                                         <  1:2642487/90‑1 (MQ=255)

                                             cAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTAGAGGAAGTCGCAACACATTGTgg                                        >  2:912213/1‑90 (MQ=255)

                                              aGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGa                                       <  1:1153907/90‑1 (MQ=255)

                                               gcgcAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGaa                                      <  2:2499477/90‑1 (MQ=255)

                                                cgcAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAAt                                     >  2:2594721/1‑90 (MQ=255)

                                                cgcAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAAt                                     <  2:101762/90‑1 (MQ=255)

                                                 gcACTCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATc                                    <  2:667456/90‑1 (MQ=255)

                                                 gcAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATc                                    >  1:1491845/1‑90 (MQ=255)

                                                  cAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCa                                   <  1:1106427/90‑1 (MQ=255)

                                                  cAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCa                                   <  1:740994/90‑1 (MQ=255)

                                                    aTCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGa                                 >  2:326901/1‑90 (MQ=255)

                                                    aTCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGa                                 >  1:582078/1‑90 (MQ=255)

                                                     tCTACAGATGCTGGAAAAAATCGGGGACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGAt                                >  2:1131089/1‑90 (MQ=255)

                                                     tCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGAt                                <  1:818648/90‑1 (MQ=255)

                                                     tCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGAt                                <  2:163188/90‑1 (MQ=255)

                                                     tCTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGAt                                <  1:214011/90‑1 (MQ=255)

                                                      cTACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGAtt                               >  1:2923653/1‑90 (MQ=255)

                                                       tACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTa                              >  1:1273911/1‑90 (MQ=255)

                                                       tACAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTa                              <  2:2308224/90‑1 (MQ=255)

                                                        aCAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTaa                             <  1:1331408/90‑1 (MQ=255)

                                                        aCAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTaa                             >  1:1511230/1‑90 (MQ=255)

                                                         cAGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTaaa                            <  1:1029765/90‑1 (MQ=255)

                                                          aGATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAg                           <  1:1170332/90‑1 (MQ=255)

                                                           gATGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGc                          >  1:3377577/1‑90 (MQ=255)

                                                            aTGCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGcc                         >  2:2257555/1‑90 (MQ=255)

                                                              gCTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCaa                       >  2:1721285/1‑90 (MQ=255)

                                                               cTGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAat                      <  2:3467620/90‑1 (MQ=255)

                                                                tGGAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAata                     >  1:1359579/1‑90 (MQ=255)

                                                                 ggAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAatat                    >  1:3149136/1‑90 (MQ=255)

                                                                 ggAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAatat                    <  1:3256050/90‑1 (MQ=255)

                                                                  gAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATg                   <  1:1593237/90‑1 (MQ=255)

                                                                  gAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATg                   >  1:1148875/1‑90 (MQ=255)

                                                                  gAAAAAATCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATg                   <  1:1407587/90‑1 (MQ=255)

                                                                    aaaaaTCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCt                 >  2:25302/1‑90 (MQ=255)

                                                                    aaaaaTCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCt                 <  1:2112670/90‑1 (MQ=255)

                                                                       aaTCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGa              >  1:155920/1‑90 (MQ=255)

                                                                       aaTCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGa              <  1:1011711/90‑1 (MQ=255)

                                                                        aTCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGat             >  1:523912/1‑90 (MQ=255)

                                                                        aTCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGat             <  1:759854/90‑1 (MQ=255)

                                                                        aTCGGGCACCTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGat             <  2:2984326/90‑1 (MQ=255)

                                                                         tCGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGata            >  2:675300/1‑90 (MQ=255)

                                                                          cGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGatat           >  1:3196696/1‑90 (MQ=255)

                                                                          cGGGCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGatat           <  2:517/90‑1 (MQ=255)

                                                                           gggCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATAtt          >  2:1417857/1‑90 (MQ=255)

                                                                           gggCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATAtt          >  1:2862270/1‑90 (MQ=255)

                                                                             gCACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTcc        >  1:1013747/1‑90 (MQ=255)

                                                                              cACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCg       <  2:2279725/90‑1 (MQ=255)

                                                                              cACTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCg       >  1:2386647/1‑90 (MQ=255)

                                                                               aCTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCgg      <  2:3036010/90‑1 (MQ=255)

                                                                               aCTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCgg      <  1:2644886/90‑1 (MQ=255)

                                                                                cTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCGGt     <  2:2424952/90‑1 (MQ=255)

                                                                                cTTTAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCGGt     <  2:2058355/90‑1 (MQ=255)

                                                                                 tttAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCGGTa    <  2:1578470/90‑1 (MQ=255)

                                                                                 tttAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCGGTa    <  1:2454986/90‑1 (MQ=255)

                                                                                  ttAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCGGTaa   <  2:920853/90‑1 (MQ=255)

                                                                                   tAAGACCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCGGTaaa  <  2:2837106/90‑1 (MQ=255)

GGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCGGTAAA  >  AC_000091/2937210‑2937382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`