BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	2,949,857	G→A	L421L (CTG→TTG)	recD ←	exonuclease V (RecBCD complex), alpha chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	2,949,857	0	G	A	100.0%	519.1 / NA	130	L421L (CTG→TTG)	recD	exonuclease V (RecBCD complex), alpha chain
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (64/66); total (64/66)

Only 100 of 134 total aligned reads displayed.

CAAACGGCCCTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAGATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGTTTTTCGATA  >  AC_000091/2949770‑2949942

cAAACGGCCCTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAAt                                                                                     <  2:2368393/90‑1 (MQ=255)

 aaaCGGCCCTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATc                                                                                    >  2:2442675/1‑90 (MQ=255)

  aaCGGCCCTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATcc                                                                                   <  2:2113444/90‑1 (MQ=255)

  aaCGGCCCTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATcc                                                                                   <  1:1846833/90‑1 (MQ=255)

   aCGGCCCTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCa                                                                                  <  2:1790041/90‑1 (MQ=255)

   aCGGCCCTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCa                                                                                  >  2:2223508/1‑90 (MQ=255)

     ggCCCTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGa                                                                                >  2:2813290/1‑90 (MQ=255)

      gCCCTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGAt                                                                               <  2:164148/90‑1 (MQ=255)

       cccTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATa                                                                              >  2:734398/1‑90 (MQ=255)

       cccTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATa                                                                              <  1:1941933/90‑1 (MQ=255)

          ttCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACg                                                                           >  1:1463326/1‑90 (MQ=255)

           tCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGt                                                                          <  1:1186872/90‑1 (MQ=255)

           tCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGt                                                                          <  1:1106220/90‑1 (MQ=255)

            cccGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTc                                                                         <  1:132871/90‑1 (MQ=255)

            cccGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTc                                                                         >  2:2359486/1‑90 (MQ=255)

             ccGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTcc                                                                        <  1:403385/90‑1 (MQ=255)

              cGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCg                                                                       <  1:28711/90‑1 (MQ=255)

              cGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCg                                                                       >  2:2491299/1‑90 (MQ=255)

              cGCAGGGCGCACAAAAGCTGGAACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCg                                                                       >  1:346503/1‑90 (MQ=255)

                 aGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTTCGTaa                                                                    >  2:1699190/1‑90 (MQ=255)

                 aGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTaa                                                                    <  2:2179978/90‑1 (MQ=255)

                 aGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTaa                                                                    <  2:3441028/90‑1 (MQ=255)

                    gcgcACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAAccc                                                                 <  1:3180533/90‑1 (MQ=255)

                    gcgcACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAAccc                                                                 >  1:301610/1‑90 (MQ=255)

                      gcACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGc                                                               >  2:2397374/1‑90 (MQ=255)

                        acaAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCaa                                                             >  2:1357567/1‑90 (MQ=255)

                         caAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAag                                                            >  1:605536/1‑90 (MQ=255)

                          aaaaGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAaga                                                           >  2:1802760/1‑90 (MQ=255)

                          aaaaGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAaga                                                           >  2:358064/1‑90 (MQ=255)

                          aaaaGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAaga                                                           <  2:2980435/90‑1 (MQ=255)

                           aaaGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAagag                                                          >  2:888168/1‑90 (MQ=255)

                            aaGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGc                                                         >  1:3202342/1‑90 (MQ=255)

                              gCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCtt                                                       >  2:782162/1‑90 (MQ=255)

                              gCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCtt                                                       >  2:955881/1‑90 (MQ=255)

                               cTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTc                                                      >  2:2524625/1‑90 (MQ=255)

                                 ggTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCt                                                    >  2:2848528/1‑90 (MQ=255)

                                  gTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTc                                                   <  2:2799515/90‑1 (MQ=255)

                                    aCTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCaa                                                 >  2:555797/1‑90 (MQ=255)

                                     cTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAg                                                >  2:2692897/1‑90 (MQ=255)

                                      tCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGc                                               >  1:2223415/1‑90 (MQ=255)

                                       cATTGAAGGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCa                                              >  2:2866094/1‑90 (MQ=255)

                                       cATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCa                                              >  1:1040432/1‑90 (MQ=255)

                                        aTTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCAt                                             >  1:1207415/1‑90 (MQ=255)

                                        aTTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCAt                                             <  2:849615/90‑1 (MQ=255)

                                        aTTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCAt                                             >  1:164424/1‑90 (MQ=255)

                                         ttGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATc                                            <  1:2599779/90‑1 (MQ=255)

                                         ttGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATc                                            <  1:1506123/90‑1 (MQ=255)

                                          tGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCGGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCg                                           >  1:1370687/1‑90 (MQ=255)

                                          tGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCg                                           <  1:1048211/90‑1 (MQ=255)

                                          tGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCg                                           <  1:2654670/90‑1 (MQ=255)

                                           gAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGc                                          >  1:2212913/1‑90 (MQ=255)

                                            aaCGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCa                                         >  1:1851648/1‑90 (MQ=255)

                                            aaCGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCa                                         <  2:1809791/90‑1 (MQ=255)

                                            aaCGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCAGCAAGAGCTTCCTCAAGCATCGCa                                         >  2:2801941/1‑90 (MQ=255)

                                             aCGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCaa                                        <  2:2456861/90‑1 (MQ=255)

                                              cGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAat                                       >  1:703814/1‑90 (MQ=255)

                                               gCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAata                                      <  1:1635887/90‑1 (MQ=255)

                                               gCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAata                                      <  1:620821/90‑1 (MQ=255)

                                                ccTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAatat                                     <  2:2502040/90‑1 (MQ=255)

                                                  tGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATaa                                   <  2:1690624/90‑1 (MQ=255)

                                                       gATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGCTAACGTCCGTCACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTc                              <  1:389772/90‑1 (MQ=255)

                                                       gATTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTc                              <  1:1872168/90‑1 (MQ=255)

                                                        aTTAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTcg                             >  1:225422/1‑90 (MQ=255)

                                                         ttAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTcgc                            <  1:1143546/90‑1 (MQ=255)

                                                         ttAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTcgc                            <  2:630252/90‑1 (MQ=255)

                                                          tAAATCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTcgcc                           >  2:3480754/1‑90 (MQ=255)

                                                           aaaTCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTcgccg                          <  1:1296908/90‑1 (MQ=255)

                                                           aaaTCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTcgccg                          <  2:507994/90‑1 (MQ=255)

                                                            aaTCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTcgccgc                         >  1:3074044/1‑90 (MQ=255)

                                                            aaTCCGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTcgccgc                         <  1:274457/90‑1 (MQ=255)

                                                              tCCGGCTCGGCGCGCGCTTGCAGCAAATCCAGATAACGTCCGTATCCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGctc                       <  2:951513/90‑1 (MQ=255)

                                                               ccGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGctct                      <  1:3127958/90‑1 (MQ=255)

                                                               ccGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGctct                      <  2:2818873/90‑1 (MQ=255)

                                                                cGGCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTg                     >  1:1959915/1‑90 (MQ=255)

                                                                 ggCTCGGCACGCGCTTGCAGCAAATCC‑‑ATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTaa                  >  1:663636/1‑90 (MQ=255)

                                                                  gCTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTa                   >  2:1178260/1‑90 (MQ=255)

                                                                   cTCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTaa                  <  1:3479414/90‑1 (MQ=255)

                                                                    tCGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTaaa                 >  1:2269973/1‑90 (MQ=255)

                                                                     cGGCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTaaaa                <  2:33657/90‑1 (MQ=255)

                                                                      ggCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAg               >  2:1640310/1‑90 (MQ=255)

                                                                       gCACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGc              <  1:20323/90‑1 (MQ=255)

                                                                        cACGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGcc             <  1:2438459/90‑1 (MQ=255)

                                                                        cACGCGCTTGCAGAAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGcc             <  1:984210/90‑1 (MQ=255)

                                                                         aCGCGCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCg            >  1:2684960/1‑90 (MQ=255)

                                                                          cgcgCTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGt           <  2:2030065/90‑1 (MQ=255)

                                                                           gcgcTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGtt          <  2:1095716/90‑1 (MQ=255)

                                                                            cgcTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGttt         <  2:1104341/90‑1 (MQ=255)

                                                                            cgcTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGttt         >  2:1709992/1‑90 (MQ=255)

                                                                            cgcTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGttt         >  1:282164/1‑90 (MQ=255)

                                                                             gcTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTTAAAGCCGtttt        <  2:1210912/90‑1 (MQ=255)

                                                                              cTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGttttt       >  2:1990192/1‑90 (MQ=255)

                                                                              cTTGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGttttt       <  1:1592233/90‑1 (MQ=255)

                                                                               ttGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGTTTTTc      >  2:191548/1‑90 (MQ=255)

                                                                               ttGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGTTTTTc      >  1:1309539/1‑90 (MQ=255)

                                                                               ttGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGTTTTTc      <  2:3363083/90‑1 (MQ=255)

                                                                                tGCAGCAAATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGTTTTTCg     >  2:515029/1‑90 (MQ=255)

                                                                                  cagcaaATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGTTTTTCGat   >  1:2318000/1‑90 (MQ=255)

                                                                                   agcaaATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGTTTTTCGata  >  2:1617744/1‑90 (MQ=255)

                                                                                   agcaaATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGTTTTTCGata  >  2:607529/1‑90 (MQ=255)

                                                                                   agcaaATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGTTTTTCGata  >  1:3204719/1‑90 (MQ=255)

CAAACGGCCCTTCCCGCAGGGCGCACAAAAGCTGGTACTCATTGAACGCCTGAATGATTAAATCCGGCTCGGCACGCGCTTGCAGCAGATCCAGATAACGTCCGTAACCCGCAAGAGCTTCCTCAAGCATCGCAATATAATCTTCGCCGCTCTGTAAAAGCCGTTTTTCGATA  >  AC_000091/2949770‑2949942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`