BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	3,123,098	A→G	C340R (TGC→CGC)	glcF ←	glycolate oxidase iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	3,123,098	0	A	G	100.0%	436.2 / NA	110	C340R (TGC→CGC)	glcF	glycolate oxidase iron‑sulfur subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (49/61); total (49/61)

Only 100 of 121 total aligned reads displayed.

CGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTT  >  AC_000091/3123011‑3123184

cGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGcgca                                                                                      <  2:2155578/90‑1 (MQ=255)

  cTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGa                                                                                    <  2:1757336/90‑1 (MQ=255)

   tttCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGAt                                                                                   <  1:1123320/90‑1 (MQ=255)

   tttCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGAt                                                                                   <  2:1381641/90‑1 (MQ=255)

   tttCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGAt                                                                                   >  2:1953541/1‑90 (MQ=255)

    ttCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATg                                                                                  <  1:3337452/90‑1 (MQ=255)

    ttCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATg                                                                                  <  1:282900/90‑1 (MQ=255)

     tCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATgg                                                                                 >  1:2744605/1‑90 (MQ=255)

       cAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCt                                                                               >  1:1843279/1‑90 (MQ=255)

           gcATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCg                                                                           <  2:2188369/90‑1 (MQ=255)

           gcATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCg                                                                           <  1:796082/90‑1 (MQ=255)

              ttcattTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGa                                                                        >  1:173504/1‑90 (MQ=255)

               tcattTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGaa                                                                       <  1:129444/90‑1 (MQ=255)

               tcattTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGaa                                                                       <  2:739434/90‑1 (MQ=255)

                 attTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACg                                                                     >  1:2529979/1‑90 (MQ=255)

                 attTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACg                                                                     >  2:762261/1‑90 (MQ=255)

                 attTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCCGGAACg                                                                     <  2:440741/90‑1 (MQ=255)

                  ttttGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGt                                                                    >  1:2874777/1‑90 (MQ=255)

                  ttttGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGt                                                                    >  2:2740106/1‑90 (MQ=255)

                  ttttGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGt                                                                    <  2:2802120/90‑1 (MQ=255)

                     tGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCg                                                                 <  1:1998130/90‑1 (MQ=255)

                     tGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCg                                                                 >  2:1682262/1‑90 (MQ=255)

                     tGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCg                                                                 <  2:2436175/90‑1 (MQ=255)

                     tGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCg                                                                 >  2:2991805/1‑90 (MQ=255)

                      gTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGt                                                                <  1:731968/90‑1 (MQ=255)

                       ttATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGtt                                                               <  1:1324581/90‑1 (MQ=255)

                       ttATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGtt                                                               >  1:3445674/1‑90 (MQ=255)

                       ttATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGtt                                                               <  2:561350/90‑1 (MQ=255)

                        tATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGAAGCGCAGATGGCTGTCGGGAACGCCCGTTa                                                              >  2:2990521/1‑90 (MQ=255)

                        tATCCCGCAGCTGGCATGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTa                                                              <  1:3149673/90‑1 (MQ=255)

                         aTCCCGCAGCTGGCGTGCCAGATCGGCATGCGTTAACGCATATGTTCCCGCTCAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTaa                                                             <  1:1270088/90‑1 (MQ=255)

                          tCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACCTCCGTTAAg                                                            <  2:2001498/90‑1 (MQ=255)

                           cccGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAgg                                                           >  1:1878780/1‑90 (MQ=255)

                           cccGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAgg                                                           <  2:1414142/90‑1 (MQ=255)

                            ccGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGt                                                          >  1:2967478/1‑90 (MQ=255)

                            ccGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGt                                                          <  1:1508537/90‑1 (MQ=255)

                             cGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTa                                                         >  2:390965/1‑90 (MQ=255)

                             cGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTa                                                         <  2:3300736/90‑1 (MQ=255)

                               cAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTaaa                                                       >  2:14882/1‑90 (MQ=255)

                                 gCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATc                                                     >  2:2930577/1‑90 (MQ=255)

                                 gCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACAGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATc                                                     >  2:158666/1‑90 (MQ=255)

                                  cTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATcc                                                    <  1:2018542/90‑1 (MQ=255)

                                   tGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCa                                                   >  1:143938/1‑90 (MQ=255)

                                    ggCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCaa                                                  >  1:1207478/1‑90 (MQ=255)

                                      cGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGa                                                >  1:1303240/1‑90 (MQ=255)

                                      cGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGa                                                >  1:2482558/1‑90 (MQ=255)

                                       gTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGAc                                               >  2:957564/1‑90 (MQ=255)

                                       gTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGAc                                               >  2:579215/1‑90 (MQ=255)

                                       gTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGAc                                               <  1:2373224/90‑1 (MQ=255)

                                           cAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAg                                           <  1:3200322/90‑1 (MQ=255)

                                            aGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGc                                          <  1:669692/90‑1 (MQ=255)

                                            aGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGc                                          <  2:2905521/90‑1 (MQ=255)

                                            aGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGc                                          <  1:229553/90‑1 (MQ=255)

                                             gATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCa                                         >  2:3088002/1‑90 (MQ=255)

                                             gATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCa                                         <  2:1115862/90‑1 (MQ=255)

                                              aTCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCaa                                        >  2:936890/1‑90 (MQ=255)

                                              aTCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCaa                                        <  2:1128662/90‑1 (MQ=255)

                                                cGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAaca                                      >  1:1423621/1‑90 (MQ=255)

                                                cGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAaca                                      <  1:3158272/90‑1 (MQ=255)

                                                 gggATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAacac                                     <  1:3433859/90‑1 (MQ=255)

                                                    aTGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACttt                                  <  1:1001874/90‑1 (MQ=255)

                                                     tGCGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACtttt                                 <  2:2609956/90‑1 (MQ=255)

                                                       cGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGATGAAGCAACACTTTTTc                               >  1:2704064/1‑90 (MQ=255)

                                                       cGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTc                               <  1:1022538/90‑1 (MQ=255)

                                                       cGTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTc                               <  2:1121888/90‑1 (MQ=255)

                                                        gTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGATGAAGCAACACTTTTTcc                              <  1:3135534/90‑1 (MQ=255)

                                                        gTTAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTcc                              <  1:1709863/90‑1 (MQ=255)

                                                         ttAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCa                             <  1:2985222/90‑1 (MQ=255)

                                                         ttAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCa                             <  1:3036835/90‑1 (MQ=255)

                                                          tAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCAc                            <  2:842634/90‑1 (MQ=255)

                                                          tAACGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCAc                            >  2:111951/1‑90 (MQ=255)

                                                             cGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTc                         >  2:3201309/1‑90 (MQ=255)

                                                             cGCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTc                         <  1:1020415/90‑1 (MQ=255)

                                                              gCATATGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCg                        >  1:1722395/1‑90 (MQ=255)

                                                                atatGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGcc                      <  1:2689681/90‑1 (MQ=255)

                                                                 tatGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCg                     >  2:437522/1‑90 (MQ=255)

                                                                 tatGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCg                     <  2:1944409/90‑1 (MQ=255)

                                                                 tatGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCg                     <  2:2862363/90‑1 (MQ=255)

                                                                   tGTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGtt                   >  2:3206870/1‑90 (MQ=255)

                                                                    gTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTc                  <  2:311127/90‑1 (MQ=255)

                                                                    gTTCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCCCCGTTc                  >  2:1060160/1‑90 (MQ=255)

                                                                     ttCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCa                 <  1:2691853/90‑1 (MQ=255)

                                                                     ttCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCa                 <  2:380553/90‑1 (MQ=255)

                                                                     ttCCCGCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCa                 <  2:2442597/90‑1 (MQ=255)

                                                                       cccGCTGAACCGCAGCGCAGATGGCTGGCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGc               >  2:254275/1‑90 (MQ=255)

                                                                          gCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCttt            >  1:2665574/1‑90 (MQ=255)

                                                                          gCTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCttt            >  2:869183/1‑90 (MQ=255)

                                                                           cTGAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCtttt           >  1:379445/1‑90 (MQ=255)

                                                                             gAACCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTgc         >  1:1144716/1‑90 (MQ=255)

                                                                              aaCCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTgcg        >  1:1246163/1‑90 (MQ=255)

                                                                               aCCGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTgcgc       <  2:453087/90‑1 (MQ=255)

                                                                                ccGCAGCGCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCa      <  1:1647658/90‑1 (MQ=255)

                                                                                 cgcagcgcagATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCAt     <  1:1408132/90‑1 (MQ=255)

                                                                                 cgcagcgcagATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCAt     <  1:1230805/90‑1 (MQ=255)

                                                                                 cgcagcgcagATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCAt     >  1:2694430/1‑90 (MQ=255)

                                                                                  gcagcgCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCAtg    >  1:92365/1‑90 (MQ=255)

                                                                                   cagcgCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCAtgt   <  2:733976/90‑1 (MQ=255)

                                                                                   cagcgCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCAtgt   >  1:2307639/1‑90 (MQ=255)

                                                                                    agcgCAGATGGCTGTCGGGAACGTCCGTTAAGGTAACTCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTCTTGCGCAtgtt  <  1:3333596/90‑1 (MQ=255)

                                                                                    agcgCAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCAtgtt  <  2:2637198/90‑1 (MQ=255)

CGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTT  >  AC_000091/3123011‑3123184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`