Predicted mutation
evidence seq id position mutation annotation gene description
RA AC_000091 3,127,881 G→A R331C (CGC→TGC)  yghO ← predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000913,127,8810GA100.0% 517.2 / NA 129R331C (CGC→TGC) yghOpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (75/54);  total (75/54)

Only 100 of 131 total aligned reads displayed.

ATGACATCTCCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCGGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTT  >  AC_000091/3127794‑3127966
                                                                                       |                                                                                     
aTGACATCTCCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGc                                                                                     >  2:2825128/1‑90 (MQ=255)
aTGACATCTCCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGc                                                                                     <  1:1473090/90‑1 (MQ=255)
  gACATCTCCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTg                                                                                   >  2:150514/1‑90 (MQ=255)
     aTCTCCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGAGCAATCACCGGGCCGATGCAGCTGAAc                                                                                >  1:2690067/1‑90 (MQ=255)
      tctcCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACt                                                                               <  2:3434301/90‑1 (MQ=255)
      tctcCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACt                                                                               <  1:3458666/90‑1 (MQ=255)
      tctcCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACt                                                                               >  2:2847141/1‑90 (MQ=255)
      tctcCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACt                                                                               >  2:110085/1‑90 (MQ=255)
       ctcCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTg                                                                              >  1:3260676/1‑90 (MQ=255)
       ctcCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTg                                                                              >  1:3395765/1‑90 (MQ=255)
        tcCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGa                                                                             >  2:2180115/1‑90 (MQ=255)
          cAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATa                                                                           >  2:399427/1‑90 (MQ=255)
             cgcATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACtc                                                                        >  1:1383619/1‑90 (MQ=255)
              gcATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACtcg                                                                       >  2:395834/1‑90 (MQ=255)
              gcATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACtcg                                                                       >  1:1315797/1‑90 (MQ=255)
                aTCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACtcgtc                                                                     >  2:1800875/1‑90 (MQ=255)
                 ttgATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACtcgtcg                                                                    <  2:454079/88‑1 (MQ=255)
                 tCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACtcgtcg                                                                    >  2:725917/1‑90 (MQ=255)
                 tCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACtcgtcg                                                                    <  1:331379/90‑1 (MQ=255)
                  cGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTcgc                                                                   >  2:2195905/1‑90 (MQ=255)
                   gATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGGAATCACCGGGCCGATGCAGCTGAACTGATACTCGTcgcg                                                                  >  1:1092618/1‑90 (MQ=255)
                   gATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTcgcg                                                                  >  2:1236264/1‑90 (MQ=255)
                   gATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTcgcg                                                                  >  1:2781444/1‑90 (MQ=255)
                    aTCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCgcgc                                                                 <  2:1812219/90‑1 (MQ=255)
                     tCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCa                                                                <  1:2366622/90‑1 (MQ=255)
                      cTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCAc                                                               >  1:2891314/1‑90 (MQ=255)
                       ttCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACg                                                              <  2:2554254/90‑1 (MQ=255)
                        tCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGc                                                             <  1:628751/90‑1 (MQ=255)
                        tCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGc                                                             >  2:2041403/1‑90 (MQ=255)
                          cggcggGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGccc                                                           >  1:2771229/1‑90 (MQ=255)
                           ggcggGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCa                                                          >  2:3300135/1‑90 (MQ=255)
                           ggcggGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCa                                                          >  1:3142222/1‑90 (MQ=255)
                              gggCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCa                                                       >  2:1224161/1‑90 (MQ=255)
                               ggCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAg                                                      <  1:3259976/90‑1 (MQ=255)
                                 cAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCg                                                    >  1:1309013/1‑90 (MQ=255)
                                 cAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCg                                                    >  2:166586/1‑90 (MQ=255)
                                    aCGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTa                                                 <  2:2974263/90‑1 (MQ=255)
                                     cGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTAc                                                <  1:1232240/90‑1 (MQ=255)
                                      ggATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCNGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACt                                               >  2:1946422/1‑90 (MQ=255)
                                      ggATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACt                                               >  1:1527749/1‑90 (MQ=255)
                                       gATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTc                                              >  2:186732/1‑90 (MQ=255)
                                        aTCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTcg                                             <  2:1421370/90‑1 (MQ=255)
                                         tCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTcgc                                            <  1:3252598/90‑1 (MQ=255)
                                          cgcgTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCCTCAGCGGTACTcgcg                                           >  2:1298454/1‑90 (MQ=255)
                                          cgcgTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTcgcg                                           <  2:547491/90‑1 (MQ=255)
                                           gcgTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCgcgc                                          >  1:808604/1‑90 (MQ=255)
                                            cgTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCa                                         <  1:1066428/90‑1 (MQ=255)
                                            cgTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCa                                         >  1:279614/1‑90 (MQ=255)
                                              tAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGt                                       <  2:3051230/90‑1 (MQ=255)
                                              tAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGt                                       <  1:2884968/90‑1 (MQ=255)
                                               aaGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGtt                                      <  2:635846/90‑1 (MQ=255)
                                               aaGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGtt                                      >  2:218276/1‑90 (MQ=255)
                                                aGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTc                                     >  1:1163110/1‑90 (MQ=255)
                                                 ggCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCg                                    <  2:2679849/90‑1 (MQ=255)
                                                 ggCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGATGAACTGATACTCGTCGCGCACGCCCATCAGCGGGACTCGCGCAGGTCg                                    >  2:2313756/1‑90 (MQ=255)
                                                   cTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCTCTTCCATCAGCGGTACTCGCGCAGTTCGca                                  <  1:2440994/90‑1 (MQ=255)
                                                   cTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGca                                  >  1:1684503/1‑90 (MQ=255)
                                                   cTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGca                                  >  1:306127/1‑90 (MQ=255)
                                                   cTTCAATCAATAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGca                                  <  2:3406800/90‑1 (MQ=255)
                                                      caatcaatAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCacac                               <  1:564275/90‑1 (MQ=255)
                                                       aatcaatAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACAcc                              >  1:2001198/1‑90 (MQ=255)
                                                        atcaatAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATTCTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCg                             <  2:1642286/90‑1 (MQ=255)
                                                        atcaatAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCg                             >  1:874682/1‑90 (MQ=255)
                                                        atcaatAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCg                             <  1:529466/90‑1 (MQ=255)
                                                         tcaatAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGc                            >  2:3284224/1‑90 (MQ=255)
                                                          caatAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCt                           <  2:2988187/90‑1 (MQ=255)
                                                          caatAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCt                           <  2:1477552/90‑1 (MQ=255)
                                                           aatAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTg                          <  1:3404838/90‑1 (MQ=255)
                                                            atAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGa                         >  1:2378435/1‑90 (MQ=255)
                                                            atAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGa                         <  1:2359231/90‑1 (MQ=255)
                                                             tAACAGCGCAATCACCGGGCCGATGCAGCTGAACTGATTCTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGAc                        <  1:3368634/90‑1 (MQ=255)
                                                                cAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACttt                     <  2:3446555/90‑1 (MQ=255)
                                                                cAGCGCAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACttt                     <  1:2998931/90‑1 (MQ=255)
                                                                    gcAATCACCGGGCCGGTGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCaaa                 >  2:2113799/1‑90 (MQ=255)
                                                                    gcAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCaaa                 >  2:3265991/1‑90 (MQ=255)
                                                                    gcAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCaaa                 >  2:1029390/1‑90 (MQ=255)
                                                                    gcAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCaaa                 >  2:2737706/1‑90 (MQ=255)
                                                                     cAATCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAAc                <  1:587016/90‑1 (MQ=255)
                                                                      aaTCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACg               <  2:2238796/90‑1 (MQ=255)
                                                                      aaTCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACg               >  1:193175/1‑90 (MQ=255)
                                                                       aTCACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGc              >  1:764058/1‑90 (MQ=255)
                                                                         cACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCca            <  2:2395481/90‑1 (MQ=255)
                                                                         cACCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCca            >  1:1913936/1‑90 (MQ=255)
                                                                          aCCGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCcac           >  2:2661659/1‑90 (MQ=255)
                                                                           ccGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCcaca          <  1:2123997/90‑1 (MQ=255)
                                                                           ccGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCACATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCcaca          >  2:1591079/1‑90 (MQ=255)
                                                                            cGGGCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTCCTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAg         <  1:867939/90‑1 (MQ=255)
                                                                             gggCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGAGGTACTCGTGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGc        >  1:2137036/1‑90 (MQ=255)
                                                                              ggCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCa       <  1:222532/90‑1 (MQ=255)
                                                                              ggCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCa       >  1:1738827/1‑90 (MQ=255)
                                                                              ggCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCa       <  1:1143300/90‑1 (MQ=255)
                                                                               gCCGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCaa      <  1:1819414/90‑1 (MQ=255)
                                                                                ccGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCAAt     >  2:2261638/1‑90 (MQ=255)
                                                                                 cGATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCAAtt    >  1:3136179/1‑90 (MQ=255)
                                                                                  gATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCAAttt   >  1:2209275/1‑90 (MQ=255)
                                                                                  gATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCAAttt   >  1:2390213/1‑90 (MQ=255)
                                                                                  gATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCAAttt   >  1:349548/1‑90 (MQ=255)
                                                                                  gATGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCAAttt   >  1:386239/1‑90 (MQ=255)
                                                                                   aTGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCAAtttt  <  2:2982168/90‑1 (MQ=255)
                                                                                   aTGCAGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCAAtttt  >  1:22671/1‑90 (MQ=255)
                                                                                       |                                                                                     
ATGACATCTCCAGCGCATCGATCTTCCGGCGGGCAAACGGATCGCGTAAGGCTTCAATCAATAACAGCGCAATCACCGGGCCGATGCGGCTGAACTGATACTCGTCGCGCACGCCCATCAGCGGTACTCGCGCAGTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTT  >  AC_000091/3127794‑3127966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑