BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	3,169,789	G→A	R217Q (CGG→CAG)	qseC →	sensory histidine kinase in two‑component regulatory system with QseB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	3,169,789	0	G	A	100.0%	505.3 / NA	126	R217Q (CGG→CAG)	qseC	sensory histidine kinase in two‑component regulatory system with QseB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (59/67); total (59/67)

Only 100 of 126 total aligned reads displayed.

CGCTGAACAAACTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCGGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGACGCAGCT  >  AC_000091/3169702‑3169874

 gcTGAACAAACTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGcc                                                                                    >  1:2456181/1‑90 (MQ=255)

      aCAAACTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTgg                                                                               >  1:1104010/1‑90 (MQ=255)

       cAAACTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGt                                                                              <  1:3035431/90‑1 (MQ=255)

        aaaCTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGtt                                                                             >  1:2132260/1‑90 (MQ=255)

        aaaCTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGtt                                                                             <  1:782961/90‑1 (MQ=255)

         aaCTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGATTGGCGTACCCAGCGAAGTGCAGCCACTGGTTg                                                                            <  2:488903/90‑1 (MQ=255)

          aCTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGa                                                                           <  1:1989238/90‑1 (MQ=255)

          aCTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGa                                                                           >  2:527121/1‑90 (MQ=255)

           cTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAANNGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAg                                                                          >  2:1846766/1‑90 (MQ=255)

           cTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAg                                                                          >  1:545052/1‑90 (MQ=255)

             ggCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTc                                                                        <  2:2093919/90‑1 (MQ=255)

             ggCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTc                                                                        <  2:2444447/90‑1 (MQ=255)

               cgcTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGc                                                                      >  1:3315465/1‑90 (MQ=255)

               cgcTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGc                                                                      >  1:509568/1‑90 (MQ=255)

                gcTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCt                                                                     <  2:2211315/90‑1 (MQ=255)

                 cTGGCACTACGTGTGCGTGCCCCTGACTCGGAAAACCCCCTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCAACTGGTTGAGTCGCTa                                                                    <  1:1288829/90‑1 (MQ=255)

                  tGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTaa                                                                   >  2:332456/1‑90 (MQ=255)

                   ggCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTaaa                                                                  <  1:2876025/90‑1 (MQ=255)

                    gCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAAt                                                                 <  1:296042/90‑1 (MQ=255)

                     cACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATc                                                                <  2:365557/90‑1 (MQ=255)

                     cACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATc                                                                <  1:150639/90‑1 (MQ=255)

                      actactTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCa                                                               <  2:3334960/90‑1 (MQ=255)

                      aCTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCa                                                               >  1:1789593/1‑90 (MQ=255)

                      aCTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCa                                                               >  2:2130425/1‑90 (MQ=255)

                         aCGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACt                                                            <  2:1967365/90‑1 (MQ=255)

                         aCGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACt                                                            <  1:2717594/90‑1 (MQ=255)

                         aCGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACt                                                            <  1:2774187/90‑1 (MQ=255)

                         aCGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACt                                                            <  2:845716/90‑1 (MQ=255)

                          cGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTg                                                           >  2:2334837/1‑90 (MQ=255)

                          cGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTg                                                           >  2:596411/1‑90 (MQ=255)

                          cGTATGCGTGACCCTGACTCGAAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTg                                                           <  1:2640885/90‑1 (MQ=255)

                           gTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGt                                                          <  2:2744904/90‑1 (MQ=255)

                           gTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGt                                                          >  2:284599/1‑90 (MQ=255)

                           gTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGt                                                          >  2:2303359/1‑90 (MQ=255)

                           gTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGt                                                          <  1:3275441/90‑1 (MQ=255)

                           gTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGt                                                          >  1:102454/1‑90 (MQ=255)

                           gTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGt                                                          <  1:2607931/90‑1 (MQ=255)

                           gTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGt                                                          <  1:2048495/90‑1 (MQ=255)

                             aTGCGTGACCCTGACTCGGAAAAACCACTAACCGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTc                                                        >  2:3378812/1‑90 (MQ=255)

                             aTGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTc                                                        <  2:2921403/90‑1 (MQ=255)

                             aTGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTc                                                        <  2:2356942/90‑1 (MQ=255)

                              tGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACAGGCGTACCCAGCGAAGTGCAGCCACAGGTTGAGTCGCTAAATCAACTGTTCg                                                       >  1:397528/1‑90 (MQ=255)

                               gCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGc                                                      >  1:3104110/1‑90 (MQ=255)

                                 gTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGccc                                                    <  1:1356479/90‑1 (MQ=255)

                                 gTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGccc                                                    >  2:1060503/1‑90 (MQ=255)

                                    aCCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGca                                                 <  1:2893664/90‑1 (MQ=255)

                                    aCCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGca                                                 <  2:3040884/90‑1 (MQ=255)

                                    aCCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGca                                                 <  2:2818584/90‑1 (MQ=255)

                                     cccTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGcac                                                >  1:839824/1‑90 (MQ=255)

                                      ccTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGcaca                                               <  1:994791/90‑1 (MQ=255)

                                      ccTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGcaca                                               <  2:1241516/90‑1 (MQ=255)

                                       cTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGcacac                                              >  2:2494376/1‑90 (MQ=255)

                                        tGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGcacaca                                             >  2:1917998/1‑90 (MQ=255)

                                         gACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACAt                                            <  1:1590560/90‑1 (MQ=255)

                                          aCTCGGAAAACCCACTAACCGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATg                                           <  1:3393175/90‑1 (MQ=255)

                                          aCTCGGAAAAACCCCTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATg                                           <  1:1025877/90‑1 (MQ=255)

                                           cTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGc                                          <  2:2690131/90‑1 (MQ=255)

                                           cTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGc                                          <  2:2481869/90‑1 (MQ=255)

                                           cTCGGAAAAACCACAAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGc                                          <  1:2958200/90‑1 (MQ=255)

                                            tCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCg                                         >  2:759194/1‑90 (MQ=255)

                                             cGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCga                                        >  1:100905/1‑90 (MQ=255)

                                                aaaaaCCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCgatga                                     <  1:2726071/90‑1 (MQ=255)

                                                aaaaaCCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCgatga                                     >  1:226650/1‑90 (MQ=255)

                                                 aaaaCCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCgatgat                                    <  1:525506/90‑1 (MQ=255)

                                                   aaCCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATgg                                  <  1:2460709/90‑1 (MQ=255)

                                                    aCCACTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGt                                 <  2:378444/90‑1 (MQ=255)

                                                        cTAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGt                             >  2:379444/1‑90 (MQ=255)

                                                         tAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTg                            >  1:2503330/1‑90 (MQ=255)

                                                         tAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTg                            <  2:3060415/90‑1 (MQ=255)

                                                         tAAACGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTg                            <  1:215929/90‑1 (MQ=255)

                                                          aaaCGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGa                           <  2:723357/90‑1 (MQ=255)

                                                            aCGCGACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAac                         <  1:2023073/90‑1 (MQ=255)

                                                             cgcgACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAacg                        <  1:1156207/90‑1 (MQ=255)

                                                               cgACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAacgac                      <  2:220281/90‑1 (MQ=255)

                                                               cgACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAacgac                      >  1:288489/1‑90 (MQ=255)

                                                               cgACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAacgac                      <  1:1381042/90‑1 (MQ=255)

                                                                gACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAacgacg                     >  2:2991836/1‑90 (MQ=255)

                                                                gACTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGATTCGTGAacgacg                     >  1:739944/1‑90 (MQ=255)

                                                                 aCTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGc                    >  2:1458523/1‑90 (MQ=255)

                                                                 aCTGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGc                    <  2:2642811/90‑1 (MQ=255)

                                                                   tGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCtt                  >  1:1353584/1‑90 (MQ=255)

                                                                   tGGCGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCtt                  <  1:1209844/90‑1 (MQ=255)

                                                                      cGTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTAc               <  1:1550929/90‑1 (MQ=255)

                                                                       gTACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTAcc              >  2:2812387/1‑90 (MQ=255)

                                                                        tACCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCt             >  2:561850/1‑90 (MQ=255)

                                                                         aCCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTc            >  2:1065143/1‑90 (MQ=255)

                                                                         aCCCAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTc            <  1:1302058/90‑1 (MQ=255)

                                                                          cccAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTcc           <  2:281284/90‑1 (MQ=255)

                                                                          cccAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTcc           <  2:2361824/90‑1 (MQ=255)

                                                                          cccAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTcc           <  1:1356683/90‑1 (MQ=255)

                                                                            cAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGa         >  2:2086153/1‑90 (MQ=255)

                                                                            cAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGa         <  2:2529053/90‑1 (MQ=255)

                                                                            cAGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGa         <  1:3295376/90‑1 (MQ=255)

                                                                             aGCGAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGAc        >  1:958951/1‑90 (MQ=255)

                                                                                gAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGACGCa     >  1:2109178/1‑90 (MQ=255)

                                                                                gAAGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGACGCa     <  1:1531969/90‑1 (MQ=255)

                                                                                 aaGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGACGCAg    >  2:3389671/1‑90 (MQ=255)

                                                                                 aaGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGACGCAg    >  2:452615/1‑90 (MQ=255)

                                                                                  aGTGCAGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGACGCAGc   >  1:728113/1‑90 (MQ=255)

                                                                                   gTGCAGCCCCTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGACGCAGCt  <  2:1006322/90‑1 (MQ=255)

CGCTGAACAAACTGGCGCTGGCACTACGTATGCGTGACCCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCGGCCACTGGTTGAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTTACCTCCGACGCAGCT  >  AC_000091/3169702‑3169874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`