BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	3,215,778	T→C	I115T (ATC→ACC)	yqjI →	predicted transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	3,215,778	0	T	C	100.0%	509.5 / NA	129	I115T (ATC→ACC)	yqjI	predicted transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (74/55); total (74/55)

Only 100 of 132 total aligned reads displayed.

AGAATCTAACCCAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCATCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACCGCGAACAGGTGG  >  AC_000091/3215691‑3215861

agaATCTAACCCAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCAccc                                                                                   >  2:105629/1‑90 (MQ=255)

  aaTCTAACCCAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCcgc                                                                                 <  2:1258850/90‑1 (MQ=255)

  aaTCTAACCCAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCcgc                                                                                 >  2:1646272/1‑90 (MQ=255)

   aTCTAACCCAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCcgcg                                                                                <  1:1327904/90‑1 (MQ=255)

    tCTAACCCAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGa                                                                               >  2:2103853/1‑90 (MQ=255)

     cTAACCCAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGaa                                                                              >  2:1109562/1‑90 (MQ=255)

     cTAACCCAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGaa                                                                              <  1:1799355/90‑1 (MQ=255)

      tAACCCAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAag                                                                             >  1:92983/1‑90 (MQ=255)

       aaCCCAGGGGAATTACACCCCAAGCCCGGGCGGCATCGACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCGGATTACCAGCCGCGAaga                                                                            >  2:3314035/1‑90 (MQ=255)

         cccAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCTGTCGCTGATTACCACCCGCGAAGagg                                                                          <  2:2529939/90‑1 (MQ=255)

         cccAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGagg                                                                          >  2:2001671/1‑90 (MQ=255)

         cccAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGagg                                                                          >  1:2758043/1‑90 (MQ=255)

         cccAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGagg                                                                          <  1:210275/90‑1 (MQ=255)

         cccAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGagg                                                                          >  1:2977022/1‑90 (MQ=255)

          ccAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGagga                                                                         <  2:2771690/90‑1 (MQ=255)

          ccAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGagga                                                                         >  2:849550/1‑90 (MQ=255)

          ccAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGagga                                                                         <  1:1093836/90‑1 (MQ=255)

            aGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGaggaag                                                                       >  1:3040317/1‑90 (MQ=255)

            aGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGCCGCTGATTACCACCCGCGAAGaggaag                                                                       <  1:2012124/90‑1 (MQ=255)

              gggAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGaggaagga                                                                     <  2:3439231/90‑1 (MQ=255)

              gggAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGaggaagga                                                                     <  2:2158850/90‑1 (MQ=255)

               ggAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAaggag                                                                    >  1:1732715/1‑90 (MQ=255)

                 aaTTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGt                                                                  >  2:3405927/1‑90 (MQ=255)

                 aaTTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGt                                                                  >  1:1906920/1‑90 (MQ=255)

                  aTTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTa                                                                 >  1:1971442/1‑90 (MQ=255)

                  aTTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTa                                                                 <  1:3213851/90‑1 (MQ=255)

                  aTTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTa                                                                 >  2:2698765/1‑90 (MQ=255)

                   ttACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTaa                                                                >  1:3114957/1‑90 (MQ=255)

                   ttACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTaa                                                                <  1:3316938/90‑1 (MQ=255)

                    tACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTaag                                                               >  1:2185610/1‑90 (MQ=255)

                     acacCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTaaga                                                              <  1:179240/90‑1 (MQ=255)

                     acacCCCAAGCCCGGGCGGCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTaaga                                                              >  2:2658686/1‑90 (MQ=255)

                       acCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTaagaag                                                            >  2:2296011/1‑90 (MQ=255)

                       acCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTaagaag                                                            >  2:645911/1‑90 (MQ=255)

                       acCCCAAGCCCGGGCGTCATCTACAAAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTaagaag                                                            >  2:1347394/1‑90 (MQ=255)

                         cccAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCa                                                          >  2:3444356/1‑90 (MQ=255)

                          ccAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAg                                                         >  1:3371775/1‑90 (MQ=255)

                            aaGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGAt                                                       >  1:1022898/1‑90 (MQ=255)

                            aaGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGAt                                                       >  1:1809101/1‑90 (MQ=255)

                             aGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGAtt                                                      >  1:2644653/1‑90 (MQ=255)

                             aGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGAtt                                                      >  2:2454488/1‑90 (MQ=255)

                             aGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGAtt                                                      <  1:1864026/90‑1 (MQ=255)

                             aGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGAtt                                                      >  1:1017903/1‑90 (MQ=255)

                               cccGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTgc                                                    <  1:2720441/90‑1 (MQ=255)

                               cccGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTgc                                                    >  1:384485/1‑90 (MQ=255)

                               cccGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTgc                                                    <  1:2049020/90‑1 (MQ=255)

                                ccGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTgcg                                                   >  2:2168082/1‑90 (MQ=255)

                                ccGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTgcg                                                   >  2:2235503/1‑90 (MQ=255)

                                 cGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCCCCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTgcgc                                                  <  2:3009956/90‑1 (MQ=255)

                                  gggCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCt                                                 >  1:985379/1‑90 (MQ=255)

                                    gCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGa                                               <  1:1151715/90‑1 (MQ=255)

                                      gTCATCTACCCATCGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGAcc                                             <  1:1768047/90‑1 (MQ=255)

                                       tCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCg                                            <  2:90415/90‑1 (MQ=255)

                                       tCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCg                                            >  1:1784503/1‑90 (MQ=255)

                                        cATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGa                                           >  1:436159/1‑90 (MQ=255)

                                        cATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGa                                           >  2:1487919/1‑90 (MQ=255)

                                         aTCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGaa                                          <  1:665349/90‑1 (MQ=255)

                                          tCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAAc                                         <  1:1077285/90‑1 (MQ=255)

                                           cTACCCCACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACa                                        >  2:751675/1‑90 (MQ=255)

                                             aCCCAACGCTGGATTTTCTGCAGGAGCACTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGCCCGAACAAg                                      <  2:2635369/90‑1 (MQ=255)

                                                   cGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGgcgcgc                                >  2:2340985/1‑90 (MQ=255)

                                                    gCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCa                               >  2:711376/1‑90 (MQ=255)

                                                     cTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAg                              <  2:3365797/90‑1 (MQ=255)

                                                     cTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAg                              >  2:3125561/1‑90 (MQ=255)

                                                     cTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAg                              <  1:2545819/90‑1 (MQ=255)

                                                     cTGGATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGCAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAg                              >  2:1523979/1‑90 (MQ=255)

                                                        gATTTTCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTgg                           >  1:2487409/1‑90 (MQ=255)

                                                          ttttCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCt                         <  1:3351146/90‑1 (MQ=255)

                                                          ttttCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCt                         <  2:1078377/90‑1 (MQ=255)

                                                           tttCTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTg                        >  1:3294914/1‑90 (MQ=255)

                                                              cTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGgaa                     <  2:1713163/90‑1 (MQ=255)

                                                              cTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGgaa                     <  2:1563247/90‑1 (MQ=255)

                                                              cTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGgaa                     >  1:3044120/1‑90 (MQ=255)

                                                              cTGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGgaa                     <  1:2688699/90‑1 (MQ=255)

                                                               tGCAGGAGCAGTCGCTGATTACCGCCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGgaag                    <  1:1153513/90‑1 (MQ=255)

                                                               tGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGgaag                    >  2:3213396/1‑90 (MQ=255)

                                                               tGCAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGgaag                    >  2:1386117/1‑90 (MQ=255)

                                                                 cAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGgaagaa                  >  2:398536/1‑90 (MQ=255)

                                                                 cAGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCCGACCGAACAAGGCGCGCAGTGGCTGgaagaa                  <  2:25464/90‑1 (MQ=255)

                                                                  aGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGaaa                 <  1:2946699/90‑1 (MQ=255)

                                                                  aGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGaaa                 >  1:1113853/1‑90 (MQ=255)

                                                                  aGGAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGaaa                 >  1:1296390/1‑90 (MQ=255)

                                                                   ggAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGaaaa                <  1:2944089/90‑1 (MQ=255)

                                                                   ggAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGaaaa                <  2:1894075/90‑1 (MQ=255)

                                                                   ggAGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGaaaa                >  2:1647207/1‑90 (MQ=255)

                                                                     aGCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAAcc              <  1:721281/90‑1 (MQ=255)

                                                                      gCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACcg             >  2:3232289/1‑90 (MQ=255)

                                                                      gCAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACcg             >  1:217307/1‑90 (MQ=255)

                                                                       cAGTCGCTGATTACCACCCGCGATGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACcgc            <  2:3398581/90‑1 (MQ=255)

                                                                       cAGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACcgc            >  1:767759/1‑90 (MQ=255)

                                                                        aGTCGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACcgcg           >  2:1654817/1‑90 (MQ=255)

                                                                           cGCTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACCGCGAAc        <  2:2641449/90‑1 (MQ=255)

                                                                             cTGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACCGCGAACAg      <  1:2658530/90‑1 (MQ=255)

                                                                              tGATTACCACCCGCGAAGAGGTAGGAGGGAAGAAGCAGATTGCGCTGACCGAACCAGGCGCGCAGTGGCTGAAAGAAAACCGCGAACAgg     >  2:90909/1‑90 (MQ=255)

                                                                              tGATTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACCGCGAACAgg     <  2:2266439/90‑1 (MQ=255)

                                                                                aTTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACCGCGAACAGGTg   >  2:2111395/1‑90 (MQ=255)

                                                                                aTTACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGATGGAAGAAAACCGCGAACAGGTg   >  2:1211776/1‑90 (MQ=255)

                                                                                 ttACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACCGCGAACAGGTgg  <  1:1234655/90‑1 (MQ=255)

                                                                                 ttACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACCGCGAACAGGTgg  <  1:3459225/90‑1 (MQ=255)

                                                                                 ttACCACCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAAACGCGAACAGGTgg  >  1:1451563/1‑90 (MQ=255)

AGAATCTAACCCAGGGGAATTACACCCCAAGCCCGGGCGTCATCTACCCAACGCTGGATTTTCTGCAGGAGCAGTCGCTGATTACCATCCGCGAAGAGGAAGGAGGTAAGAAGCAGATTGCGCTGACCGAACAAGGCGCGCAGTGGCTGGAAGAAAACCGCGAACAGGTGG  >  AC_000091/3215691‑3215861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`